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Dual role of lipids for genome stability and pluripotency facilitates full potency of mouse embryonic stem cells

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蛋白质与细胞:英文版 2023年第8期14卷 591-602页

作者： Liangwen Zhong Miriam Gordillo Xingyi Wang Yiren Qin Yuanyuan Huang Alexey Soshnev Ritu Kumar Gouri Nanjangud Daylon James C.David Allis todd Evans Bryce Carey Duancheng Wen Department of Reproductive Medicine Ronald O.Perelman and Claudia Cohen Center for Reproductive MedicineWeill Cornell MedicineNew YorkNY 10065USA Department of Surgery Weill Cornell Medical College1300 York AvenueNew YorkNY 10065USA Laboratory of Chromatin Biology and Epigenetics The Rockefeller UniversityNew YorkNY 10065USA Cold Spring Harbor Laboratory Cold Spring HarborNY 11724USA Molecular Cytogenetics Core.Memorial Sloan Kettering Cancer Center New YorkNY 10065USA Department of Neuroscience Developmental and Regenerative BiologyUniversity of Texas at San AntonioOne UTSA CircleSan AntonioTX 78249USA Gladstone Institutes 1650 Owens StSan FranciscoCA94158USA

While Mek1/2 and Gsk3βinhibition("2i")supports the maintenance of murine embryonic stem cells(EsCs)in a homogenous naive state,prolonged culture in 2i results in aneuploidy and DNA hypomethylation that impairs developmental ***,2i fails to support derivation and culture of fully potent female *** we find that mouse ESCs cultured in 2i/LIF supplemented with lipid-rich albumin(AlbuMAx)undergo pluripotency transition yet maintain genomic stability and full potency over long-term ***,lipids in AlbuMAx impact intracellular metabolism including nucleotide biosynthesis,lipid biogenesis,and TCA cycle intermediates,with enhanced expression of DNMT3s that prevent DNA *** induce a formative-like pluripotent state through direct stimulation of Erk2 phosphorylation,which also alleviates X chromosome loss in female ***,both male and female"all-ESc"mice can be generated from de novo derived ESCs using AlbuMAXbased *** findings underscore the importance of lipids to pluripotency and link nutrient cues to genome integrity in early development.

关键词： mouse pluripotent stem cells lipids pluripotency transition genomic stability developmental potency nucleotide pool depletion 2i medium X chromosome loss femaleall-EsC mice

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The Troupe(Excerpt)

Frontiers of Literary Studies in China-Selected Publications from Chinese Universities 2018年第4期12卷 645-673页

作者： WANG Anyi todd Foley 不详 Department of East Asian Studies & Comparative Literature New York UniversityNew YorkNY 10003USA

*** OperaIn its previous life,this regional Cultural Workers' Troupe of ours had been a Liuzi opera *** being displaced by the 'new culture,' Liuzi opera seemed to be in full decline—an ancient and rare dramatic form that had become *** had never seen it ourselves,or even heard a line of it *** said it had its origins as palace entertainment,possessing a subtle richness and lofty style that made it a truly elite form,though too elite to gain the popularity needed to withstand the test of *** explanation always sounded a little overblown.

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Effect of longwall-induced subsurface deformations on shale gas well casing stability under deep covers

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International Journal of Mining Science and Technology 2019年第1期29卷 3-8页

作者： Daniel W.H.Su Peter Zhang Mark Van Dyke todd Minoski Center for Disease Control and Prevention(CDC) National Institute for Occupational Safety and Health(NIOSH)Pittsburgh Mining Research Division(PMRD)

This paper presents the results of a 2017 study conducted by the National Institute for Occupational Safety and Health(NIOSH), Pittsburgh Mining Research Division(PMRD), to evaluate the effects of longwall-induced subsurface deformations within a longwall abutment pillar under deep cover. The 2017 study was conducted in a southwestern Pennsylvania coal mine, which extracts 457 m-wide longwall panels under 361 m of cover. One 198 m-deep, in-place inclinometer monitoring well was drilled and installed over a 45 m by 84 m center abutment pillar. In addition to the monitoring well, surface subsidence measurements and underground coal pillar pressure measurements were conducted as the 457 m-wide longwall panel on the south side of the abutment pillar was being mined. Prior to the first longwall excavation, a number of simulations using FLAC3D^(TM) were conducted to estimate surface subsidence, increases in underground coal pillar pressure, and subsurface horizontal displacements in the monitoring well. Comparisons of the pre-mining FLAC3D simulation results and the surface, subsurface,and underground instrumentation results show that the measured in-place inclinometer casing deformations are in reasonable agreement with those predicted by the 3D finite difference models. The measured surface subsidence and pillar pressure are in excellent agreement with those predicted by the 3D *** from this 2017 research clearly indicate that, under deep cover, the measured horizontal displacements within the abutment pillar are approximately one order of magnitude smaller than those measured in a 2014 study under medium cover.

关键词： Longwall Deformations Shale gas wells Casing Safety and health

ERCP for the treatment of bile leak after partial hepatectomy and fenestration for symptomatic polycystic liver disease

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World Journal of Gastroenterology 2012年第28期18卷 3705-3709页

作者： Nayantara Coelho-Prabhu David M Nagorney todd H Baron Mayo Clinic College of Medicine Rochester MN 55905 United States

AIM: To describe endoscopic treatment of bile leaks in these patients and to identify risk factors in these patients which can predict the development of bile leaks. METHODS: Retrospective case-control study examining consecutive patients who underwent partial hepatectomy for polycystic liver disease (PLD) and developed a postoperative bile leak managed endoscopically over a ten year period. Each case was matched with two controls with PLD who did not develop a postoperative bile leak. RESULTS: Ten cases underwent partial hepatectomy with fenestration for symptoms including abdominal distention, pain and nausea. Endoscopic retrograde cholangiopancreatography (ERCP) showed anatomic abnormalities in 1 case. A biliary sphincterotomy was performed in 4 cases. A plastic biliary stent was placed with the proximal end at the site of the leak in 9 cases; in 1 case two stents were placed. The overall success rate of ERCP to manage the leak was 90%. There were no significant differences in age, gender, comorbidities, duration of symptoms, history of previous surgery or type of surgery performed between cases and controls. CONCLUSION: ERCP with stent placement is safe and effective for management of post-hepatectomy bile leak in patients with PLD.

关键词：切除术症状治疗开窗肝持续时间 PLD 危险因素

Analysis of Oxides Formed on the Surface of the Alloy 690 in Hydrogenated Supercritical Water

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Acta Metallurgica Sinica(English Letters) 2016年第8期29卷 774-781页

作者： Jin Gao Fa-rong Wan Guo-ping Cao Kumar Sridharan todd Allen School of Materials Science and Engineering University of Science and Technology Beijing Department of Materials Science and Engineering University of Wisconsin-Madison

The oxides formed on the surface of the alloy 690 in hydrogenated supercritical water at 400℃ for 1000 h were investigated using scanning electron microscopy,transmission electron microscopy,scanning transmission electron microscopy and energy-dispersive X-ray *** oxides on me surface of the alloy 690 exhibited multi-layer structure：an outer layer consisted of granular crystallites（NiO and NiFe_2O_4） and a compact inner layer（spinel and Cr_2O_3）.Chemical analysis indicated that the outer layer was enriched in nickel but depleted in chromium,whereas the inner layer was enriched in chromium and iron but depleted in *** inner layer was also characterized as layered structure by Fe-rich spinel on top of continuous Cr_2O_3 ***,Cr_2O_3 nodules were readily observed at the oxides/alloy interface.

关键词： Alloy 690 Supercritical water Oxides Corrosion

Influence of varying bedding thickness of underclay on floor stability

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International Journal of Mining Science and Technology 2017年第3期27卷 517-523页

作者： Jessu Kashi Vishwanath Kostecki todd Ray Spearing Anthony John Spencer Zhang Jixiong Western Australia School of Mines Curtin University Kalgoorlie 6430 Australia Southern Illinois University Carhondale 62901 USA The State Key Laboratory of Coal Resources and Safe Mining China University of Mining & Technology Xuzhou 221116 China

The variation in bedding thickness of the weak immediate floor has long been a challenge in the Illinois basin coal mines when it comes to floor stability. The vertical thickness of the immediate floor is not constant throughout the mines and can vary over short horizontal distances. The biggest misconception from a design standpoint is to use the maximum or average thickness found from core logs taken from various locations on the mine property. The result of this practice is oversized pillars in the areas where the weak immediate floor has thinned vertically. This over-design leaves coal in situ which could have otherwise been extracted. This paper presents a plane strain numerical model to illustrate the effect of a change in bedding thickness of a weak immediate floor across one or two coal pillars. The floor bearing capacity of the variable floor below each pillar where then compared to the consistent floor. The results show that the varying bedding thickness of weak underclay has an impact on the bearing capacity of the *** with the decrease in bedding thickness for constant pillar width, the B/H ratio increases exponentially. The influence of varying bedding thickness on the floor bearing capacity is apparent at higher B/H ratios. The floor bearing capacity under a single pillar is in variable floor model if the average thickness remains constant. For single pillar, the average of the bedding thickness can be considered and for pillars in a panel, and a safety factor has been proposed to take into account this change in bedding thickness.

关键词： Bedding thickness Underclay Fireclay Illinois Basin Floor bearing capacity Floor safety factor

FunnelCloud:a cloud-based system for exploring tornado events

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International Journal of Digital Earth 2017年第10期10卷 1030-1054页

作者： Jie Lian Michael P.McGuire todd W.Moore Department of Computer and Information Sciences Towson UniversityTowsonMDUSA Department of Geography and Environmental Planning Towson UniversityTowsonMDUSA

Recent research has shown an increase in the number of extreme tornado outbreaks per *** characterization of the spatio-temporal pattern of tornado events is therefore a critical task in the analysis of meteorological ***,there are a large number of available meteorological datasets that can be used for such ***,much of these data are distributed across multiple websites and are not accessible in a central *** poses a significant challenge for a scientist who is interested in exploring meteorological patterns associated with tornado *** paper presents a novel system which uses cloud-based technology for integrating,storing,exploring,analyzing,and visualizing meteorological data associated with tornado *** system employs a novel NoSQL database schema and web services architecture for data integration and provides a user friendly interface that allows scientists to explore the spatio-temporal pattern of tornado ***,scientists can use this interface to analyze the relationship between different meteorological variables and properties of tornado outbreaks using a number of spatio-temporal statistical and data mining *** efficacy of the system is demonstrated on a use case centered on the analysis of climatic indicators of large spatio-temporally clustered tornado outbreaks.

关键词： tornado data warehouse cloud-based system NoSQL data integration spatio-temporal clustering web-mapping

Successful endoscopic treatment of colonic gallstone ileus using electrohydraulic lithotripsy

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World Journal of Gastroenterology 2010年第12期16卷 1533-1536页

作者： Martin D Zielinski Lincoln E Ferreira todd H Baron Division of Trauma Critical Care and General Surgery Department of Medicine Federal University of Juiz de Fora Department of Gastroenterology and Hepatology

The surgical management of gallstone ileus is complex and potentially highly *** management requires enterolithotomy and is generally followed by fistula resection at a later *** have been reports of gallstone extraction using various endoscopic modalities to relieve the obstruction,however,to date,there has never been a published case of endoscopic stone extraction from the colon using electrohydraulic *** this report,we present the technique employed to successfully perform an electrohydraulic lithotripsy for removal of a large gallstone impacted in the sigmoid colon.A cavity was excavated in an obstructing 4.1 cm lamellated stone in the sigmoid colon using electrohydraulic lithotripsy.A screw stent retractor and stent extractor bored a larger lumen which allowed for guidewire advancement and stone fracture via serial pneumatic balloon *** stone fragments were *** lithotripsy is a safe and effective method to treat colonic obstruction in the setting of gallstone ileus.

关键词： Gallstone ileus Endoscopy Electrohydraulic lithotripsy Bowel obstruction Colon

Exploring Chemovar-Specific Cannabis Extracts Quantification and Evaluation of Cytotoxic Compounds for Targeting Glioblastoma Multiforme

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Journal of Biosciences and Medicines 2023年第8期11卷 192-207页

作者： Ashraf Duzan Mufeed Basti Travis Cesarone Waldemar Debinski Daniel todd Wingate University School of Pharmacy Wingate University Wingate NC USA Applied Science and Technology Department North Carolina State University of Agriculture and Technology Greensboro NC USA Department of Pharmaceutical Sciences College of Pharmacy Health Professions Division Nova Southeastern University Fort Lauderdale USA Uprooted Concepts British Columbia Victoria Canada Brain Tumor Center of Excellence Wake Forest School of Medicine Medical Center Blvd Winston Salem NC USA Department of Chemistry and Biochemistry The University of North Carolina at Greensboro Greensboro NC USA

Glioblastoma Multiforme (GBM) represents one of the most aggressive and metastatic brain tumors, with a dismal success rate of less than three percent after five years, particularly in tumors with active immune checkpoints. This necessitates the development of targeted endogenous agents for precise GBM treatment. Previous experiments utilizing Chemovar Specific Cannabis Extractions (CSCEs), fractionated with polar solvents and quantified using Liquid and Gas Column Chromatography combined with Mass Spectrometry (LC/GCMS), have shown reduced viability and motility in human GBM cell lines. However, the complexity of the botanical substance has hindered the personalization of standard cannabis medicines for GBM due to unknown synergistic effects of multiple compounds. To address this limitation, our study focuses on exposing AM251 cells to chemovar fractions extracted using a non-polar solvent, thereby isolating a broader spectrum of constituents. By employing LC/GCMS in conjunction with Nuclear Magnetic Resonance (NMR), we have identified and quantified nine* compounds present in the non-polar CSCE that exhibit significant efficacy (0.1 μM) in inducing cytotoxicity* in GBM tumor cells. Conversely, the polar fraction in our experiment did not demonstrate efficacy against UM251 cells. The quantification of individual compounds within a cannabis extraction that selectively induces cell death in brain tumors holds promise for guiding future research and facilitating the development of a standardized CSCE for GBM therapy.

关键词： Cannabinoids Liquid Chromatography Mass Spectrometry (LC-MS) Glioblastoma Multiforme (GBM) Total Correlation Spectroscopy (TOCSY) Gas Chromatography-Mass Spectrometry (GC-MS)