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High-throughput multiplexed fluorescence-activated droplet sorting

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microsystems & Nanoengineering 2018年第1期4卷 95-104页

作者： Ouriel Caen Simon Schütz m.S.Suryateja Jammalamadaka Jérémy Vrignon Philippe Nizard tobias m.schneider Jean-Christophe Baret Valérie Taly INSERm UmR-S1147 CNRS SNC5014Paris Descartes UniversityEquipe labellisée Ligue Nationale contre le cancerParisFrance Emergent Complexity in Physical Systems Laboratory(ECPS) Ecole Polytechnique Fédérale de Lausanne1015 LausanneSwitzerland CNRS University BordeauxCRPPUMR 5031115 Avenue Schweitzer33600 PessacFrance

Fluorescence-activated droplet sorting(FADS)is one of the most important features provided by droplet-based ***,to date,it does not allow to compete with the high-throughput multiplexed sorting capabilities offered by flow ***,we demonstrate the use of a dielectrophoretic-based FADS,allowing to sort up to five different droplet populations *** system provides means to select droplets of different phenotypes in a single experimental run to separate initially heterogeneous *** experimental results are rationalized with the help of a numerical model of the actuation of droplets in electric fields providing guidelines for the prediction of sorting designs for upscaled or downscaled microsystems.

关键词： rational initially sorting

APPROXImATING THE STATIONARY BELLmAN EQUATION BY HIERARCHICAL TENSOR PRODUCTS

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Journal of Computational mathematics 2024年第3期42卷 638-661页

作者： mathias Oster Leon Sallandt Reinhold schneider Technische Universität Berlin Strasse des 17.Juni 13510623 BerlinGermany

We treat infinite horizon optimal control problems by solving the associated stationary Bellman equation numerically to compute the value function and an optimal feedback *** dynamical systems under consideration are spatial discretizations of non linear parabolic partial differential equations(PDE),which means that the Bellman equation suffers from the curse of *** non linearity is handled by the Policy Iteration algorithm,where the problem is reduced to a sequence of linear equations,which remain the computational bottleneck due to their high *** reformulate the linearized Bellman equations via the Koopman operator into an operator equation,that is solved using a minimal residual *** the Koopman operator we identify a preconditioner for operator equation,which deems essential in our numerical *** overcome computational infeasability we use low rank hierarchical tensor product approximation/tree-based tensor formats,in particular tensor trains(TT tensors)and multi-polynomials,together with high-dimensional quadrature,*** controlling a destabilized version of viscous Burgers and a diffusion equation with unstable reaction term numerical evidence is given.

关键词： Feedback control Dynamic programming Hamilton-Jacobi-Bellman Tensor product approximation Variational monte-Carlo

modeling displacement flow inside a full-length casing string for well cementing

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Petroleum Research 2024年第1期9卷 1-16页

作者： Hu Dai Ali Eslami Jason schneider Gefei Liu Fred Schwering Pegasus Vertex Inc.USA Laval University Canada Sanjel Energy Services Canada Athabasca Oil Corporation Canada

While computer modeling of annular displacement efficiency is widely applied in cementing engineering,modeling the displacement flow inside a casing or drill string for cementing operations has received less *** predicting displacement efficiency inside a full-length pipe is desired by cementing engineers,the attempt of developing a model with both efficiency and accuracy faces *** to computer simulators for this purpose is *** with annular flow,the displacement flow inside pipe,although within a simpler geometry and without eccentricity effect,is not simpler in physics,modelling strategy and predictability,because a variety of flow patterns and flow instabilities can develop to create complicated fluid *** this paper,we present an integrated numerical model developed to simulate displacement flows inside a full-length pipe,which connects an existing annulus model to enable complete displacement simulations of cementing *** model uses three-dimensional grid to solve fluid concentrations with degrees of mixing,and incorporates flow instability detection and flow regime *** in cementing,the model accounts for effects of pumping rate,well inclination,pipe rotation,fluid densities,rheological parameters and *** computationally efficient model does not rely on high-resolution mesh as often required by conventional Computational Fluid Dynamics models,thus it is suitable to be implemented in a cementing software for daily use by well cementing *** methodology of the model is discussed in detail in this *** validate the model,we examine simulation results against experimental results obtained in our laboratory tests and CFD simulations;acceptable agreement is found under different testing *** also presented two case studies of real cementing jobs with cement evaluation logs compared to simulation results,showing that the model can predict consistent displacement efficie

关键词： Inside pipe displacement Displacement efficiency Numerical model Primary cementing Displacement flow

Two-photon emission from a superlattice-based superconducting light-emitting structure

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Light(Science & Applications) 2024年第7期13卷 1331-1340页

作者： Shlomi Bouscher Dmitry Panna Ronen Jacovi Fauzia Jabeen Christian schneider Sven Höfling Alex Hayat Department of Electrical Engineering TechnionHaifa 32000Israel. Technische Physik Universität WürzburgAm HublandD-97074 WürzburgGermany. Institute of Physics Carl von Ossietzky Universität OldenburgD-26111 OldenburgGermany

Superconductor-semiconductor hybrid devices can bridge the gap between solid-state-based and photonics-based quantum systems,enabling new hybrid computing schemes,offering increased scalability and *** example for a hybrid device is the superconducting light-emitting diode(SLED).SLEDs have been theoretically shown to emit polarization-entangled photon pairs by utilizing radiative recombination of Cooper ***,the twophoton nature of the emission has not been shown experimentally *** demonstrate two-photon emission in a GaAs/AlGaAs *** electroluminescence spectra reveal unique two-photon superconducting features below the critical temperature(Tc),while temperature-dependent photon-pair correlation experiments(g(2)(τ,T))demonstrate temperature-dependent time coincidences below Tc between photons emitted from the *** results pave the way for compact and efficient superconducting quantum light sources and open new directions in light-matter interaction studies.

关键词： photon quantum utilizing

Hyper spectral resolution stimulated Raman spectroscopy with amplified fs pulse bursts

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Light(Science & Applications) 2024年第3期13卷 566-576页

作者： Hongtao Hu tobias Flöry Vinzenz Stummer Audrius Pugzlys markus Zeiler Xinhua Xie Aleksei Zheltikov Andrius Baltuška Photonics Institute Technische Universität WienGußhausstraße 27-29Vienna A-1040Austria SwissFEL Paul Scherrer InstituteVilligen PSI 5232Switzerland Institute for Quantum Science and Engineering Department of Physics and AstronomyTexas A&M UniversityCollege StationTX 77843USA

We present a novel approach for Stimulated Raman Scattering(SRS)spectroscopy in which a hyper spectral resolution and high-speed spectral acquisition are achieved by employing amplified offset-phase controlled fs-pulse *** investigate the method by solving the coupled non-linear Schrödinger equations and validate it by numerically characterizing SRS in molecular nitrogen as a model *** spectral resolution of the method is found to be determined by the inverse product of the number of pulses in the burst and the intraburst pulse *** SRS spectrum is obtained through a motion-free scanning of the offset phase that results in a sweep of the Raman-shift *** to high spectral resolution and fast motion-free scanning the technique is beneficial for a number SRS-based applications such as gas sensing and chemical analysis.

关键词： compound spectral resolution

Femoral Access with Ultrasound-Guided Puncture and Z-Stitch Hemostasis for Adults with Congenital Heart Diseases Undergoing Electrophysiological Procedures

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Congenital Heart Disease 2024年第1期19卷 85-92页

作者： Fu Guan matthias Gass Florian Berger Heiko schneider Firat Duru Thomas Wolber Arrhythmia and Electrophysiology Division Department of CardiologyUniversity Heart Center ZurichZurichSwitzerland Children’s Research Center Zurich University Children’s HospitalZurichSwitzerland Center for Integrative Human Physiology University of ZurichZurichSwitzerland

Aims:Although the application of ultrasound-guided vascular puncture and Z-stitch hemostasis to manage femoral access has been widely utilized,there is limited data on this combined application in adult congenital heart disease(ACHD)patients undergoing electrophysiological(EP)*** sought to evaluate the safety and efﬁcacy of ultrasound-guided puncture and postprocedural Z-stitch hemostasis for ACHD patients under-going EP *** and Results:The population of ACHD patients undergoing transfemoral EP pro-cedures at the University of Zurich Heart Center between January 2019 and December 2022 was observed and *** the study period,femoral access(left/right,arterial/venous)was performed under real-time ultrasound *** the end of the procedure,a single Z-stitch was performed at the puncture *** eval-uated the incidence of in-hospital complications associated with femoral access puncture in this *** 101 patients who had a total of 147 previous ipsilateral vascular punctures(mean 1.5 per person),100 patients underwent successful femoral vascular access for EP *** median age of the patients was 47±15 years and 34(34%)were male.Z-stitches were performed after the procedure in 100 patients with 303 femoral vascular accesses(mean 3 punctures per person).No patient developed vascular puncture relevant inguinal hematoma,pseudo aneurysm,arteriovenousﬁstula,venous or arterial ***:In ACHD patients undergoing EP procedures,optimal femoral access management can be achieved with ultra-sound-guided puncture and postprocedural Z-stitch hemostasis.

关键词： Congenital heart disease cardiac electrophysiology cardiac catheterization femoral access hemostasis

Development of an active-site titrant for SARS-CoV-2 main protease as an indispensable tool for evaluating enzyme kinetics

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Acta Pharmaceutica Sinica B 2024年第5期14卷 2349-2357页

作者： Rabea Voget Julian Breidenbach tobias Claff Alexandra Hingst Katharina Sylvester Christian Steinebach Lan Phuong Vu Renato H.Weiße Ulrike Bartz Norbert Sträter Christa E.müller michael Gütschow Pharmaceutical Institute Pharmaceutical&Medicinal ChemistryUniversity of BonnBonn 53121Germany Institute of Bioanalytical Chemistry Center for Biotechnology and BiomedicineLeipzig UniversityLeipzig 04103Germany Department of Natural Sciences University of Applied Sciences Bonn-Rhein-SiegRheinbach 53359Germany

A titrant for the SARS-CoV-2 main protease(m^(pro))was developed that enables,for the first time,the exact determination of the concentration of the enzymatically active m^(pro) by active-site *** covalent binding mode of the tetrapeptidic titrant was elucidated by the determination of the crystal structure of the enzyme–titrant *** fluorogenic substrates of m^(pro),including a prototypical,internally quenched Dabcyl-EDANS peptide,were compared in terms of solubility under typical assay *** exploiting the new titrant,key kinetic parameters for the m^(pro)-catalyzed cleavage of these substrates were determined.

关键词： COVID-19 SARS-CoV-2 main protease Peptide nitriles Fluorogenic substrates Active-site titration X-ray crystallography Inner filter effect

Exome sequencing identifies a likely causative variant in 53%of families with ciliopathy-related features on renal ultrasound after excluding NPHP1 deletions

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Genes & Diseases 2024年第5期11卷 40-43页

作者： Konstantin Deutsch Verena Klambt Thomas m.Kitzler Tilman Jobst-Schwan Ronen schneider Florian Buerger Steve Seltzsam Sherif El Desoky Jameela A.Kari Farkhanda Hafeez maria Szczepanska Loai A.Eid Hazem S.Awad muna Al-Saffar Neveen A.Soliman Velibor Tasic Camille Nicolas-Frank Kirollos Yousef Luca m.Schierbaum Sophia schneider Abdul Halawi Izzeldin Elmubarak Katharina Lemberg Shirlee Shril Shrikant m.mane Nancy Rodig Friedhelm Hildebrandt Division of Nephrology Department of PediatricsBoston Children’s HospitalHarvard Medical SchoolBostonMA 02215USA Department of Pediatric Gastroenterology Nephrology and Metabolic DiseasesCharite Universitatsmedizin BerlinBerlin 13353Germany Berlin Institute of Health at Charitee Universitatsmedizin Berlin BIH Biomedical Innovation AcademyBIH Charite Clinician Scientist ProgramBerlin 10178Germany Department of Nephrology and Hypertension FriedrichAlexander-Universitat Erlangen-NurnbergErlangen 91054Germany Department of Pediatrics Faculty of Medicine King Abdulaziz UniversityPediatric Nephrology Center of ExcellenceKing Abdulaziz University HospitalJeddah 21589Saudi Arabia Department of Pediatric Nephrology The Children’s Hospital and Institute of Child HealthLahore 54000Pakistan Department of Pediatrics Faculty of Medical Sciences in ZabrzeMedical University of Silesia in KatowiceKatowice 40-752Poland Dubai Hospital and Al-Jalila Children’s Specialty Hospital Kidney Center of ExcellenceDubai 4545United Arab Emirates Department of Genetics and Genomics UAE UniversityAbu Dhabi 15551United Arab Emirates Department of Pediatrics Division of Genetics and GenomicsBoston Children’s HospitalHarvard Medical SchoolBostonMA 02215USA Department of Pediatrics Center of Pediatric Nephrology and TransplantationKasr Al Ainy School of MedicineCairo UniversityCairo 11562Egypt Egypt Center for Research and Regenerative medicine(ECRRm) Cairo 11511Egypt medical Faculty Skopje University Children’s HospitalSkopje 1000North Macedonia Department of Genetics Yale University School of MedicineNew HavenCT 06520USA

Nephronophthisis-related ciliopathies(NPHP-RC)represent one of the most common causes of chronic kidney disease in the first three decades of life and are characterized by a broad genetic and clinical heterogeneity.1 To date,more than 90 genes have been identified that cause autosomalrecessive NPHP-RC if mutated,accounting for up to 60%of cases.1 Among these,homozygous deletions of NPHP1 are the most common *** genes localize to primary cilia,basal bodies,or the centrosome and lead to a primary ciliary disruption if mutated,thereby causing a broad phenotypical spectrum.1 Patients that suffer from NPHP-RC can either have an isolated renal phenotype,such as polyuria,polydipsia,decreased urinary concentration ability,and secondary enuresis due to loss of tubular function,or present with extrarenal symptoms including retinal degeneration,cerebellar vermis hypoplasia,or hepatic *** are often diagnosed in early adolescence,reaching end-stage renal disease before 25 years of age,but early onset and rapidly progressive forms of NPHP-RC also exist.1 Renal ultrasound indicates kidneys of normal or reduced renal length with increased echogenicity and corticomedullary cysts.

关键词： degeneration urinary likely

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部分饱和岩石中流体斑块演化的定量建模

部分饱和岩石中流体斑块演化的定量建模