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Covalent functionalization of decellularized tissues accelerates endothelialization

Bioactive Materials 2021年第11期6卷 3851-3864页

作者： Eleonora Dal Sasso Annj Zamuner Andrea Filippi Filippo Romanato tiziana palmosi Luca Vedovelli Dario Gregori Jose Luís Gòmez Ribelles Teresa Russo Antonio Gloria Laura Iop Gino Gerosa Monica Dettin Department of Cardiac Thoracic and Vascular Sciences and Venetian Institute of Molecular MedicinePaduaItaly Department of Industrial Engineering University of PaduaPaduaItaly LIFELAB Program Consorzio per la Ricerca SanitariaCORISVeneto RegionItaly Department of Physics and Astronomy“G.Galilei” University of PaduaPaduaItaly Fondazione Bruno Kessler TrentoItaly Institute of Pediatric Research Citt`a della Speranza PaduaItaly Center for Biomaterials and Tissue Engineering CBITUniversitat Polit`ecnica de Val`enciaVal`enciaSpain Biomedical Research Networking Center on Bioengineering Biomaterials and Nanomedicine(CIBER-BBN)ValenciaSpain Institute of Polymers Composites and BiomaterialsNational Research Council of ItalyNaplesItaly

In the field of tissue regeneration,the lack of a stable endothelial lining may affect the hemocompatibility of both synthetic and biological *** drawbacks might be prevented by specific biomaterial functionalization to induce selective endothelial cell(EC)*** bovine pericardia and porcine aortas were selectively functionalized with a REDV tetrapeptide at 10^(-5)M and 10^(-6)M working *** scaffold-bound peptide was quantified and REDV potential EC adhesion enhancement was evaluated in vitro by static seeding of human umbilical vein *** viable cells and MTS production were statistically higher in functionalized tissues than in *** histoarchitecture,geometrical features,and mechanical properties were unaffected by peptide *** selective immobilization of REDV was effective in accelerating ECs adhesion while promoting proliferation in functionalized decellularized tissues intended for blood-contacting applications.

关键词： Decellularized pericardium Decellularized aorta Covalent functionalization Endothelialization REDV Mechanical analysis

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