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ZmMS1/ZmLBD30-orchestrated transcriptional regulatory networks precisely control pollen exine development

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Molecular Plant 2023年第8期16卷 1321-1338页

作者： Quancan Hou Xueli An Biao Ma Suowei Wu Xun Wei tingwei yan yan Zhou Taotao Zhu Ke Xie Danfeng Zhang Ziwen Li Lina Zhao Canfang Niu yan Long Chang Liu WeiZhao FeiNi Jinping Li Daolin Fu Zhong-Nanyang Xiangyuan Wan Research Institute of Biology and Agriculture University of Science and Technology BeijingBeijing 100083China Beijing Engineering Laboratory of Main Crop Bio-Tech Breeding Zhongzhi International Institute of Agricultural BiosciencesBeijing 100192China State Key Laboratory of Crop Biology College of AgronomyShandong Agricultural UniversityTaianShandong 271018China Shanghai Key Laboratory of Plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 200234China

Because of its significance for plant male fertility and,hence,direct impact on crop yield,pollen exine development has inspired decades of scientific ***,the molecularmechanismunderlying exine formation and thickness remains *** this study,we identified that a previously unrecognized repressor,ZmMS1/ZmLBD30,controls proper pollen exine development in *** an ms1 mutant with aberrantly thickened exine,we cloned a male-sterility gene,ZmMs1,which encodes a tapetum-specific lateral organ boundary domain transcription factor,*** thatZmMs1/ZmLBD30 is initially turned on by a transcriptional activation cascade of ZmbHLH51-ZmMYB84-ZmMS7,and then it serves as a repressor to shut down this cascade via feedback repression to ensure timely tapetal degeneration and proper level of *** activation-feedback repression loop regulating male fertility is conserved in maize and sorghum,and similar regulatory mechanism may also exist in other flowering plants such as rice and ***,these findings reveal a novel regulatory mechanism of pollen exine development by which a long-sought master repressor of upstream activators prevents excessive exine formation.

关键词： ZmMs1/ZmLBD30 LBD transcription factor feedback repression exine male sterility maize

ZmMs30 Encoding a Novel GDSL Lipase Is Essential for Male Fertility and Valuable for Hybrid Breeding in Maize

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Molecular Plant 2019年第3期12卷 343-359页

作者： Xueli An Zhenying Dong Youhui Tian Ke Xie Suowei Wu Taotao Zhu Danfeng Zhang Van Zhou Canfang Niu Biao Ma Quancan Hou Jianxi Bao Simiao Zhang Ziwen Li yanbo Wang tingwei yan Xiaojing Sun Yuwen Zhang Jinping Li Xiangyuan Wan Biology and Agriculture Research Center University of Science and Technology BeijingBeijing 100024China Beijing Engineering Laboratory of Main Crop Bio-Tech Breeding Beijing International Science and Technology Cooperation Base of Bio-Tech BreedingBeijing Solidwill Sci-Tech Co.Ltd.Beijing 100192China

Genic male sterility (GMS) is very useful for hybrid vigor utilization and hybrid seed production. Although a large number of GMS genes have been identified in plants, little is known about the roles of GDSL lipase members in anther and pollen development. Here, we report a maize GMS gene, ZmMs30, which encodes a novel type of GDSL lipase with diverged catalytic residues. Enzyme kinetics and activity assays show that ZmMs30 has lipase activity and prefers to substrates with a short carbon chain. ZmMs30 is specifically expressed in maize anthers during stages 7-9. Loss of ZmMs30 function resulted in defective anther cuticle, irregular foot layer of pollen exine, and complete male sterility. Cytological and lipidomics analyses demonstrate that ZmMs30 is crucial for the aliphatic metabolic pathway required for pollen exine formation and anther cuticle development. Furthermore, we found that male sterility caused by loss of ZmMs30 function was stable in various inbred lines with different genetic background, and that it didn't show any negative effect on maize heterosis and production, suggesting that ZmMs30 is valuable for crossbreeding and hybrid seed production. We then developed a new multi-control sterility system using ZmMs30 and its mutant line, and demonstrated it is feasible for generating desirable GMS lines and valu. able for hybrid maize seed production. Taken together, our study sheds new light on the mechanisms of anther and pollen development, and provides a valuable male-sterility system for hybrid breeding maize.

关键词： ZmMs30f GDSL lipase multi-control sterility (MCS) system hybrid seed production maize

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探析网络直播未来发展新可能

传媒论坛 2021年第1期4卷 41-42页

作者：闫婷玮华南理工大学广东广州510006

近年来,网络直播凭仗其便捷性、及时性、趣味性等诸多优点一经推出倍受网友青睐且迅速蹿红,如今已然发展成为具备强大经济魔力的新兴产业。突发的繁荣景象是一把双刃剑,大量的直播内容触及淫秽色情、诈骗传销、低俗谣言等底线,不仅违背... 详细信息

近年来,网络直播凭仗其便捷性、及时性、趣味性等诸多优点一经推出倍受网友青睐且迅速蹿红,如今已然发展成为具备强大经济魔力的新兴产业。突发的繁荣景象是一把双刃剑,大量的直播内容触及淫秽色情、诈骗传销、低俗谣言等底线,不仅违背了社会公德甚至触犯了法律。此行业如果想要经久不衰、枝繁叶茂地发展下去,优化网络直播大环境刻不容缓,网络直播的"绿水青山"新时代众人期盼。

关键词：网络直播传播效应网络主播直播平台

蒿芩清胆汤治疗社区获得性肺炎(湿热内郁型)的疗效及对血清炎症因子、血气指标的影响

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四川中医 2021年第5期39卷 81-84页

作者：严廷巍吕华西宁市中医院青海西宁810003 青海省中医院青海西宁810000

目的:观察蒿芩清胆汤对社区获得性肺炎(湿热内郁型)患者疗效及对血清炎症因子、血气指标的影响。方法:选择2018年1月~2019年2月我院社区获得性肺炎(辨证为湿热内郁型)患者98例,随机分为2组,各49例,对照组采用左氧氟沙星治疗;观察组采用... 详细信息

目的:观察蒿芩清胆汤对社区获得性肺炎(湿热内郁型)患者疗效及对血清炎症因子、血气指标的影响。方法:选择2018年1月~2019年2月我院社区获得性肺炎(辨证为湿热内郁型)患者98例,随机分为2组,各49例,对照组采用左氧氟沙星治疗;观察组采用蒿芩清胆汤治疗,每日1剂,2周为1疗程。比较两组间的临床疗效、血清降钙素原(Procalcitonin, PCT)、白介素-1β(Interleukin-1β,IL-1β)、肿瘤坏死因子α(Tumor Necrosis Factor-α,TNF-α)及动脉血二氧化碳分压(Arterial Partial Pressure of Carbon Dioxide, PaCO2)、动脉血氧分压(Arterial Partial Pressure of Oxygen, PaO2)及血氧饱和度(Oxygen Saturation of Blood, SpO2)水平。结果:对照组有效率79.59%低于观察组93.88%,差异有统计学意义(P<0.05)。与对照组比,观察组治疗后血清PCT、IL-1β、TNF-α水平较低,PaCO2较低,PaO2、SpO2较高,差异有统计学意义(P<0.05)。结论:蒿芩清胆汤对社区获得性肺炎(湿热内郁型)患者的疗效显著,可以降低炎症因子,改善血气指标,且安全性高,值得推广。

关键词：蒿芩清胆汤社区获得性肺炎湿热内郁型炎症因子血气指标

Genetic analysis of transcription factors in dopaminergic neuronal development in Parkinson’s disease

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Chinese Medical Journal 2024年第4期137卷 450-456页

作者： Yuwen Zhao Lixia Qin Hongxu Pan tingwei Song Yige Wang Xiaoxia Zhou Yaqin Xiang Jinchen Li Zhenhua Liu Qiying Sun Jifeng Guo Xinxiang yan Beisha Tang Qian Xu Department of Neurology Xiangya HospitalCentral South UniversityChangshaHunan 410008China Department of Neurology The Second Xiangya HospitalCentral South UniversityChangshaHunan 410011China Bioinformatics Center National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan 410008China Department of Geriatrics Xiangya HospitalCentral South UniversityChangshaHunan 410008China Centre for Medical Genetics and Hunan Key Laboratory of Medical Genetics School of Life SciencesCentral South UniversityChangshaHunan 410008China Key Laboratory of Hunan Province in Neurodegenerative Disorders Central South UniversityChangshaHunan 410008China

Background:Genetic variants of dopaminergic transcription factor-encoding genes are suggested to be Parkinson’s disease(PD)risk factors;however,no comprehensive analyses of these genes in patients with PD have been ***,we aimed to genetically analyze 16 dopaminergic transcription factor genes in Chinese patients with ***:Whole-exome sequencing(WES)was performed using a Chinese cohort comprising 1917 unrelated patients with familial or sporadic early-onset PD and 1652 ***,whole-genome sequencing(WGS)was performed using another Chinese cohort comprising 1962 unrelated patients with sporadic late-onset PD and 1279 ***:We detected 308 rare and 208 rare protein-altering variants in the WES and WGS cohorts,***-based association analyses of rare variants suggested that MSX1 is enriched in sporadic late-onset ***,the significance did not pass the Bonferroni ***,72 and 1730 common variants were found in the WES and WGS cohorts,***,single-variant logistic association analyses did not identify significant associations between common variants and ***:Variants of 16 typical dopaminergic transcription factors might not be major genetic risk factors for PD in Chinese ***,we highlight the complexity of PD and the need for extensive research elucidating its etiology.

关键词： Parkinson’s disease Transcription factors Dopaminergic neurons Genetic Variants

ZmMs25 Encodes an Active Fatty Acyl Reductase Tequired for Anther Lipid Biosynthesis and Male Fertility in Maize

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ZmMs25 Encodes an Active Fatty Acyl Reductase Tequired for A...

媒介融合背景下非物质文化遗产产业创新策略研究——以粤剧及其衍生文化为例

第十九届中国作物学会学术年会

作者： Simiao Zhang Suowei Wu Canfang Niu Dongcheng Liu tingwei yan Ke Xie Ziwen Li yanbo Wang Wei Zhao Zhenying Dong Biao Ma Xueli An Jinping Li Xiangyuan Wan Zhongzhi International Institute of agricultural Biosciences Biology and Agriculture Research Center University of Science and Technology Beijing

【Background】Fatty acyl reductases(FARs) catalyze the reduction of fatty acyl-coenzyme A or-acyl carrier protein substrates to primary fatty alcohols, which plays essential roles in plant lipid metabolism. However, molecular mechanism of FARs involved in male development is poorly defined. Here, we found that two maize allelic mutants, ms25-6065 and ms25-6057, displayed defective anther development, impaired pollen formation and complete male sterility. 【 Method 】 Cytological observation and microscopy, genetic analysis and map-based cloning, protein alignment and phylogenetic analysis, subcellular localization, microsynteny analysis, prokaryotic expression and activity assay, RNA extraction and expression analysis.【Results】Ms25 mutation affects anther cuticle and Ubisch body formation as well as microspore development. ZmMs25 and its orthologs may arise from an ancient FAR appearing before the differential evolution of monocot and dicot species. Some of maize paralogous FARs may participate in silk and anther development, and their roles in anther development may depend on the normal function of ZmMs25. ZmMs25 encoded a plastid-localized FAR with catalytic activities to multiple acyl-coenzyme A substrates in vitro, and all four conserved residues(G101, G104, Y327 and K331) in two motifs of ZmMs25 protein were critical for its enzyme activity. Moreover, loss of ZmMs25 function had significant effects on the expression patterns of its two paralogous genes and seven cloned lipid metabolic male-sterility genes in maize during anther development. Therefore, ZmMs25 plays key roles in male fertility possibly through participating in multiple lipid metabolic pathways essential for anther and pollen development. 【Conclusion】First, ZmMs25 functions as an active FAR with multiple substrates and all four conserved amino acid residues are indispensable for its catalytic activity. Second, we proposed a work model of Zm Ms25 and investigated its effects on the expressions of other

关键词： anther and pollen development fatty acyl reductase genic male sterility lipid biosynthesis maize ZmMs25

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艺术科技 2021年第6期34卷 136-138页

作者：张金梅闫婷玮华南理工大学设计学院广东广州510641

作为享有活化石美誉的文化遗产,粤剧演变发展的历史脉络与演员和观众的审美意蕴交互传承,传播具有乡土题材的俗世之美与本土地域的独特风格的文化。本文从地域的思想观念、历史文化入手,在媒介融合视角下从传承保护等多方面对粤剧及其... 详细信息

作为享有活化石美誉的文化遗产,粤剧演变发展的历史脉络与演员和观众的审美意蕴交互传承,传播具有乡土题材的俗世之美与本土地域的独特风格的文化。本文从地域的思想观念、历史文化入手,在媒介融合视角下从传承保护等多方面对粤剧及其衍生文化加以研究,以求对粤剧文化产业创新性发展研究提供助力。

关键词：粤剧文化数字媒体媒介融合

基于单孔包层与薄膜组成FP干涉仪腔的新型光纤应力传感器研究

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原子能科学技术 2022年第S1期56卷 243-248页

作者：许昌张兴虎涂玮陈柏众邹跃丁小康牟学峰闫亭伟同方中科超光科技有限公司北京102200 中国科学院理化技术研究所北京100190

本文研究了一种新型基于单孔包层与薄膜组成Fabry-Perot(FP)干涉仪腔的光纤应力传感器,单孔的一个表面为45°以使光线传输从横向转到纵向,从而使一半的包层与有机薄膜组成FP干涉仪腔。采用频率多路复用的方法获得了11个传感器复用在一... 详细信息

本文研究了一种新型基于单孔包层与薄膜组成Fabry-Perot(FP)干涉仪腔的光纤应力传感器,单孔的一个表面为45°以使光线传输从横向转到纵向,从而使一半的包层与有机薄膜组成FP干涉仪腔。采用频率多路复用的方法获得了11个传感器复用在一个系统的频谱。在采用合适的不同有机薄膜厚度的情况下,系统能集成更多的光纤传感器。研究结果表明该新型的基于单孔的光纤传感器在核工业领域实时监测方面具有潜在应用。

关键词：光纤传感器 Fabry-Perot 干涉仪多路复用应力

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基于***的网上购物系统的模块化设计与实现

电子技术（上海） 2015年第11期42卷 41-44页

作者：李茹王增圻颜廷伟曲阜师范大学工学院山东省日照市276826

文章在***平台上采用B/S结构进行设计,运用c#开发语言以及后台数据库SQL Server等关键技术模拟网上购物系统。系统采用模块化的设计原则,将该模拟系统分解成一系列功能模块,每个模块能够独立的开发和测试。这种设计原则使系统程序结构... 详细信息

文章在***平台上采用B/S结构进行设计,运用c#开发语言以及后台数据库SQL Server等关键技术模拟网上购物系统。系统采用模块化的设计原则,将该模拟系统分解成一系列功能模块,每个模块能够独立的开发和测试。这种设计原则使系统程序结构层次更清晰,同时也更方便测试和改进。据功能设计要求,本购物系统总体上分为前台销售模块与后台管理模块,其中前台是购买者购物平台,后台是商家管理平台。本文主要的创新点是整个系统中关键技术的使用、网上购物的具体功能模块的实现以及网站界面的设计。

关键词： ***平台 SQL server数据库网上购物模块化设计