检索结果-南通市图书馆

Journal of Environmental Sciences 2024年第2期136卷 270-278页

作者： tingting ku Jindong Hu Mengmeng Zhou Yuanyuan Xie Yutong Liu Xin Tan Lin Guo Guangke Li Nan Sang College of Environmental Science and Resources Research Center of Environment and HealthShanxi UniversityTaiyuan 030006China

Tebuconazole exposure has been described as an increasing hazard to human health.An increasing number of recent studies have shown a positive association between tebuconazole exposure and cardiovascular disease risk,which is characterized by the reduction of adenosine triphosphate(ATP)synthesis.However,researches on the damage of tebuconazole exposure to energy metabolism and the related molecular mechanisms are limited.In the present study,male C57BL/6 mice were treated with tebuconazole at different low concentrations for 4 weeks.The results indicated that tebuconazole could accumulate in the heart and further induce the decrease of ATP content in the mouse heart.Importantly,tebuconazole induced an obvious shift in substrate utilization of fatty acid and glucose by disrupting their corresponding transporters(GLUT1,GLUT4,CD36,FABP3 and FATP1)expression,and significantly repressed the expression of mitochondrial biogenesis(Gabpa and Tfam)and oxidative phosphorylation(CS,Ndufa4,Sdhb,Cox5a and Atp5b)related genes in a dosedependent manner.Further investigation revealed that these alterations were related to the IRS1/AKT and PPARγ/RXRαpathways.These findings contribute to a better understanding of triazole fungicide-induced cardiovascular disease by revealing the key indicators associated with this phenomenon.

关键词： Tebuconazole Cardiotoxicity Energy metabolic disorders Energy substrate Mitochondrial damage

Perfluorinated iodine alkanes promote the differentiation of mouse embryonic stem cells by regulating estrogen receptor signaling

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2024年第3期137卷 443-454页

作者： Zhihua Ren Xiaoxi Yang tingting ku Qian S.Liu Jiefeng Liang Qunfang Zhou Francesco Faiola Guibin Jiang State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China College of Resources and Environment University of Chinese Academy of SciencesBeijing 100049China College of Environment and Resource Research Center of Environment and HealthShanxi UniversityTaiyuan 030006China College of Sino-Danish University of Chinese Academy of SciencesBeijing 100049China

Investigating the development toxicity of perfuorinated iodine alkanes(PFIs)is critical,given their estrogenic effects through binding with estrogen receptors(ERs).In the present study,two PFIs,including dodecafuoro-1,6-diiodohexane(PFHx DI)and tridecafuorohexyl iodide(PFHx I),with binding preference to ERαand ERβ,respectively,were selected to evaluate their effects on proliferation and differentiation of the mouse embryonic stem cells(m ESCs).The results revealed that,similar to E_(2),50μmol/L PFHx DI accelerated the cell proliferation of the m ESCs.The PFI stimulation at the exposure concentrations of 2–50μmol/L promoted the differentiation of the m ESCs as characterized by the upregulation of differentiation-related biomarkers(i.e.,Otx2 and Dnmt3β)and downregulation of pluripotency genes(i.e.,Oct4,Nanog,Sox2,Prdm14 and Rex1).Comparatively,PFHx DI exhibited higher induction effect on the differentiation of the m ESCs than did PFHx I.The tests on ER signaling indicated that both PFI compounds induced exposure concentration-dependent expressions of ER signaling-related biomarkers(i.e.,ERα,ERβand Caveolin-1)in the m ESCs,and the downstream ER responsive genes(i.e.,c-fos,c-myc and c-jun)well responded to PFHx I stimulation.The role of ER in PFI-induced effects on the m ESCs was further validated by the antagonistic experiments using an ER inhibitor(ICI).The findings demonstrated that PFIs triggered ER signaling,and perturbed the differentiation program of the m ESCs,causing the potential health risk during early stage of development.

关键词： Perfuorinated iodine alkanes(PFIs) Embryonic stem cells(ESCs) Developmental toxicity Cell differentiation Estrogen receptor-related signaling

PM2.5 exposure induces age-dependent hepatic lipid metabolism disorder in female mice

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2020年第3期32卷 227-237页

作者： Ruifeng Yan tingting ku Huifeng Yue Guangke Li Nan Sang College of Environment and Resource Research Center of Environment and HealthShanxi UniversityTaiyuanShanxi 030006China

Particulate matter exposure has been described to elevate the risk of lung and cardiovascular diseases.An increasing number of recent studies have indicated positive correlations between PM2.5(the fraction of airborne particles with an aerodynamic diameter less than2.5 μm) exposure and the risk of liver diseases.However,research on the effects of PM2.5exposure on liver fat synthesis,secretion,and clearance mechanisms under normal diet conditions is limited,and whether these effects are age-dependent is largely unknown.Female C57BL/6 mice at different ages(4 weeks(4 w),4 months(4 m),and 10 months(10 m))were treated with 3 mg/kg body weight of PM2.5 every other day for 4 weeks.Subsequently,the ultrastructural changes of liver,the expression of genes involved in oxidative damage and lipid metabolism in the liver were examined.Observation of hepatic ultrastructure showed more and larger lipid droplets in the livers of 4-week-old and 10-month-old mice exposed to PM2.5.Further analysis showed that PM2.5 exposure increased the expression of genes related to lipid synthesis,but decreased the expression of genes involved in lipid transport and catabolism in the livers of 10-month-old mice.Our findings suggest that exposure to PM2.5 disrupts the normal metabolism of liver lipids and induces lipid accumulation in the liver of female mice in an age-dependent manner,with older mice being more susceptible to PM2.5.

关键词： Fine particulate matter Liver Lipid metabolism Different ages

Abnormal energy metabolism and tau phosphorylation in the brains of middle-aged mice in response to atmospheric PM2.5 exposure

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2017年第12期29卷 145-153页

作者： Rui Gao tingting ku Xiaotong Ji Yingying Zhang Guangke Li Nan Sang College of Environment and Resource Research Center of Environment and HealthShanxi UniversityTaiyuan 030006China

In light of the accelerated aging of the global population and the deterioration of the atmosphere pollution, we sought to clarify the potential mechanisms by which fine particulate matter（PM_（2.5）） can cause cognitive impairment and neurodegeneration through the alteration of mitochondrial structure and function. The results indicate that PM_（2.5） inhalation reduces ATP production by disrupting the aerobic tricarboxylic acid cycle and oxidative phosphorylation, thereby causing the hypophosphorylation of tau in the cortices of middle-aged mice. Furthermore, excessive reactive oxygen species generation was involved in the impairment. Interestingly, these alterations were partially reversed after exposure to PM_（2.5） ended. These findings clarify the mechanism involved in mitochondrial abnormality-related neuropathological dysfunction in response to atmospheric PM_（2.5） inhalation and provide an optimistic sight for alleviating the adverse health outcomes in polluted areas.

关键词： Fine particulate matter（PM_（2.5）） Mitochondrial structure and function Energy metabolism Reactive oxygen species（ROS） Tau phosphorylation

High-throughput discovery of highly selective reversible hMAO-B inhibitors based on at-line nanofractionation

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2024年第4期14卷 1772-1786页

作者： Yu Fan Jincai Wang Jingyi Jian Yalei Wen Jiahao Li Hao Tian Jacques Crommen Wei Bi tingting Zhang Zhengjin Jiang Institute of Pharmaceutical Analysis/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research/International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education(MOE)of China/College of Pharmacy Jinan UniversityGuangzhou 510632China ku Leuven-University of Leuven Pharmaceutical AnalysisDepartment of Pharmaceutical and Pharmacological SciencesLeuven 3000Belgium Laboratory of Analytical Pharmaceutical Chemistry Department of Pharmaceutical SciencesCIRMUniversity of LiegeLiege B-4000Belgium Department of Neurology the First Affiliated Hospital of Jinan University/Clinical Neuroscience Institutethe First Affiliated Hospital of Jinan UniversityGuangzhou 510630China

Human monoamine oxidase B(hMAO-B)has emerged as a pivotal therapeutic target for Parkinson's disease.Due to adverse effects and shortage of commercial drugs,there is a need for novel,highly selective,and reversible hMAO-B inhibitors with good blood-brain barrier permeability.In this study,a high-throughput at-line nanofractionation screening platform was established with extracts from Chuanxiong Rhizoma,which resulted in the discovery of 75 active compounds,including phenolic acids,volatile oils,and phthalides,two of which were highly selective novel natural phthalide hMAO-B inhibitors that were potent,selective,reversible and had good blood‒brain permeability.Molecular docking and molecular dynamics simulations elucidated the inhibition mechanism.Sedanolide(IC_(50)=103 nmol/L;SI=645)and neocnidilide(IC_(50)=131 nmol/L;SI=207)demonstrated their excellent potential as hMAO-B inhibitors.They offset the limitations of deactivating enzymes associated with irreversible hMAO-B inhibitors such as rasagiline.In SH-SY5Y cell assays,sedanolide(EC_(50)=0.962μmol/L)and neocnidilide(EC_(50)=1.161μmol/L)exhibited significant neuroprotective effects,comparable to the positive drugs rasagiline(EC_(50)=0.896μmol/L)and safinamide(EC_(50)=1.079μmol/L).These findings underscore the potential of sedanolide as a novel natural hMAO-B inhibitor that warrants further development as a promising drug candidate.

关键词： Human monoamine oxidase B inhibitors High-throughput screening At-linenanofractionation Parkinson's disease Cellular neuroprotection Traditional Chinese medicines(TCMs)

Growing Knowledge of Stem Cells as a Novel Experimental Model in Developmental Toxicological Studies

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2023年第3期39卷 342-360页

作者： REN Zhihua ku tingting REN Mengyao LIANG Jiefeng NING Xia XU Hanqing REN Danqin ZHOU Qunfang SANG Nan College of Environment and Resource Research Center of Environment and HealthShanxi UniversityTaiyuan030006P.R.China State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing100085P.R.China Shandong Key Laboratory of Environmental Processes and Health School of Environmental Science and EngineeringShandong UniversityQingdaoQingdao266237P.R.China National and Local Joint Engineering Research Center of Ecological Treatment Technology for Urban Water Pollution Wenzhou UniversityWenzhou325035P.R.China

The increasing production and extensive application of chemicals have led to their unintended release and contamination in the environment,posing a hazardous threat to wildlife and human health.Numerous studies have demonstrated that developmental toxicity could be induced by various emerging chemicals,causing abnormal embryonic and placental development,adverse pregnancy outcomes,obesity,and dysfunction of lipid metabolism in neonates.Given the currently-available experimental technology for developmental toxicological studies,an in vitro model based on stem cells showed promising performance in high-throughput screening of the early-stage developmental toxicity of emerging chemicals.In this review,the deleterious effects of environmental pollutants on stem cells were systemically assorted from the aspects of cytological dysfunction,self-renewal impairment,perturbation in embryoid body(EB)formation,and disruption of committed lineage differentiation.The toxicological data on the molecular level,including the altered expressions of gene and protein biomarkers,epigenomic regulation,and enhanced oxidative stress,were collected and summarized to provide the mechanism explanation for the link between environmental pollutant exposure and unfavorable phenotypes in stem cells.The advantage of the stem cell model in developmental toxicological studies was specifically emphasized.And the perspectives for stem cells were ultimately highlighted in the research field of environmental toxicology,especially developmental toxicology during the early stage of life.

关键词： Environmental pollutant Developmental toxicology Stem cell Early-stage life Health effect

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

猪CD1d蛋白多克隆抗体的制备及应用

中国农业科学 2024年第8期57卷 1620-1628页

作者：刘传霞陈欣王晓李雪雯李婷婷翁长江郑君中国农业科学院哈尔滨兽医研究所/动物疫病预防控制国家重点实验室/国家非洲猪瘟专业实验室/基础免疫创新团队

【目的】制备猪源CD1d的多克隆抗体，为探究猪CD1d蛋白在非洲猪瘟病毒（African swine fever virus,ASFV）感染过程中的功能奠定基础。【方法】利用PCR方法扩增了猪CD1d基因，并将其同源重组至pGEX-6p1载体中，构建了原核重组表达质粒pG... 详细信息

【目的】制备猪源CD1d的多克隆抗体，为探究猪CD1d蛋白在非洲猪瘟病毒（African swine fever virus,ASFV）感染过程中的功能奠定基础。【方法】利用PCR方法扩增了猪CD1d基因，并将其同源重组至pGEX-6p1载体中，构建了原核重组表达质粒pGEX-6p1-CD1d。将重组质粒转化E.coli BL21(DE3)并用IPTG进行诱导表达，表达的GST-CD1d重组蛋白经SDS-PAGE和Western blot(WB)方法鉴定，SDS-PAGE结果显示约50 ku处有一条明显的条带，该蛋白以包涵体形式表达。然后利用谷胱甘肽琼脂糖亲和层析方法进行蛋白纯化，将纯化的GST-CD1d蛋白与等体积的弗氏完全佐剂混合乳化后，将纯化的蛋白免疫新西兰大白兔，采用颈背部多点皮下注射，免疫剂量为200μg/只，首免后第3周和第5周分别进行二免和三免，均采用弗氏不完全佐剂乳化，方法和剂量与首免相同。三免后第7天，通过耳缘静脉采血分离血清。首免后第7周进行第四次免疫，一周后心脏采血。该抗体经Protein G亲和层析纯化后冻存于-80℃冰箱。通过WB和间接免疫荧光（IFA）鉴定瞬时转染表达的外源CD1d蛋白和猪原代巨噬细胞（PAMs）表达的内源CD1d蛋白的表达与细胞定位情况。同样，制备的CD1d抗体可以将外源瞬时转染表达的CD1d通过IP拉下。为了探究CD1d在ASFV感染早期的情况，将ASFV接种于PAMs，制备ASFV感染0、15、30、60 min的样品，以CD1d为一抗，通过WB检测了CD1d蛋白的表达情况。在HEK293T细胞共转pCAGGS-HA-CD1d和pCAGGS-Flag-CD2v质粒，24 h后收取细胞裂解，加入Flag beads过夜结合蛋白，通过WB检测互作情况染，同时，质粒共转染于共聚焦小皿中的HEK293T细胞，用标签抗体对其进行孵育，选择相应的荧光二抗，通过激光共聚焦显微镜观察CD1d与CD2v在细胞中共定位情况。采用Co-IP验证CD1d与ASFV外囊膜蛋白CD2v的相互作用。【结果】原核表达的GST-CD1d蛋白以包涵体形式表达在感受态细胞中，分子质量约为35 ku；实验兔4次免疫CD1d重组蛋白后采血并分离血清，纯化的抗体经SDS-PAGE检测在45和25 ku处各出现一条特异性条带，分别为CD1d抗体的重链与轻链。以纯化的CD1d蛋白作为免疫原制备的兔抗CD1d抗体包含重链和轻链，且具有较好纯度；该抗体能够通过WB和IFA鉴定瞬时转染的外源以及PAMs内源CD1d蛋白的表达和细胞定位。进一步检测结果显示，ASFV感染PAMs后，CD1d蛋白表达水平明显增加，并且WB和IFA结果显示CD1d与ASFV编码的外囊膜蛋白CD2v存在相互作用和共定位。【结论】通过原核表达技术制备了CD1d的抗体，为进一步探究CD1d蛋白在ASFV感染过程中的生物学功能打下了基础。

关键词： CD1d蛋白原核表达多克隆抗体非洲猪瘟病毒 CD2v蛋白

PARP-1在染料木素作用小鼠卵泡发生进程中的表达特征

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

江西农业大学学报 2024年第2期46卷 438-446页

作者：郭卓倩王金卢凤兰苟廷廷孟秋仙尧燕环吴华东吴国云江西农业大学动物科学技术学院/南昌市动物健康与安全生产重点实验室江西南昌330045 江西省兴国县农业技术推广中心江西赣州342400

【目的】旨在探究PARP-1信号及其介导的PAR化修饰水平在染料木素促进卵泡发育和抑制卵泡闭锁进程中的相关性,丰富染料木素等类雌激素物质作用于雌性哺乳动物卵巢卵泡发生的作用机制,以期为种畜高产日粮配方的研制提供科学指导。【方法】... 详细信息

【目的】旨在探究PARP-1信号及其介导的PAR化修饰水平在染料木素促进卵泡发育和抑制卵泡闭锁进程中的相关性,丰富染料木素等类雌激素物质作用于雌性哺乳动物卵巢卵泡发生的作用机制,以期为种畜高产日粮配方的研制提供科学指导。【方法】以21 d雌性昆明小鼠为试验动物,采用腹腔注射25,50,100 mg/kg不同剂量染料木素,每日定点注射1次,连续7 d,采集卵巢组织和血清,采用免疫组织化学法检测PARP-1蛋白在卵巢组织内的表达特征,并运用Western blot对卵巢组织内PARP-1蛋白及pADPr进行定量。结合凋亡指示蛋白Cleaved-caspase3、雌二醇(E2)水平、类固醇合成酶CYP19A1和HSD17B1,综合分析PARP-1信号及其介导的PAR化修饰信号是如何参与染料木素抑制卵泡闭锁、促进卵泡发育进程。【结果】免疫组织化学染色显示,PARP-1阳染主要发生在原始卵泡、初级卵泡、次级卵泡、有腔卵泡中的健康颗粒细胞核和卵母细胞,卵泡膜细胞次之,但在Cleaved-caspase3阳染的凋亡细胞中PARP-1不表达。Western blot结果显示,50 mg/kg染料木素处理组PARP-1解离片段(89 ku)的表达量较对照组显著降低(Pku)表达量在各剂量组无显著差异(P>0.05)。其次,50 mg/kg染料木素处理组的pADPr的表达量在15 ku以下、25 ku以及35~45 ku这3个区域内均显著低于对照组、25 mg/kg和100 mg/kg剂量组(Pku区域处表达量较对照组和25 mg/kg剂量组显著降低(P0.05)。【结论】PARP-1广泛存在于哺乳动物卵巢内健康的颗粒细胞、卵泡细胞和膜细胞中,可能通过催化pADPr的合成,介导卵巢内的能量代谢、细胞凋亡以及类固醇激素合成等胞内活动,参与染料木素等类雌激素物质干扰卵巢卵泡发生进程。

关键词：小鼠染料木素卵泡发生 PARP-1 PAR化

维普期刊数据库博看期刊

An Equivalent Substitute Strategy for Constructing 3D Ordered Porous Carbon Foams and Their Electromagnetic Attenuation Mechanism

在线全文

学校读者我要写书评

暂无评论

Nano-Micro Letters 2022年第10期14卷 15-35页

作者： Meng Zhang Hailong Ling Ting Wang Yingjing Jiang Guanying Song Wen Zhao Laibin Zhao tingting Cheng Yuxin Xie Yuying Guo Wenxin Zhao Liying Yuan Alan Meng Zhenjiang Li College of Materials Science and Engineering College of Electromechanical EngineeringQingdao University of Science and TechnologyQingdao 266061People’s Republic of China State Key Laboratory Base of Eco-Chemical Engineering College of Chemistry and Molecular EngineeringCollege of Chemical Engineering in Gaomi CampusQingdao University of Science and TechnologyQingdao 266042People’s Republic of China

Three-dimensional(3D)ordered porous carbon is generally believed to be a promising electromagnetic wave(EMW)absorbing material.However,most research works targeted performance improvement of 3D ordered porous carbon,and the specific attenuation mechanism is still ambiguous.Therefore,in this work,a novel ultra-light egg-derived porous carbon foam(EDCF)structure has been successfully constructed by a simple carbonization combined with the silica microsphere template-etching process.Based on an equivalent substitute strategy,the influence of pore volume and specific surface area on the electromagnetic parameters and EMW absorption properties of the EDCF products was confirmed respectively by adjusting the addition content and diameter of silica microspheres.As a primary attenuation mode,the dielectric loss originates from the comprehensive effect of conduction loss and polarization loss in S-band and C band,and the value is dominated by polarization loss in X band and ku band,which is obviously greater than that of conduction loss.Furthermore,in all samples,the largest effective absorption bandwidth of EDCF-3 is 7.12 GHz under the thickness of 2.13 mm with the filling content of approximately 5 wt%,covering the whole ku band.Meanwhile,the EDCF-7 sample with optimized pore volume and specific surface area achieves minimum reflection loss(RL_(min))of−58.08 dB at 16.86 GHz while the thickness is 1.27 mm.The outstanding research results not only provide a novel insight into enhancement of EMW absorption properties but also clarify the dominant dissipation mechanism for the porous carbon-based absorber from the perspective of objective experiments.

关键词： Porous carbon foam Electromagnetic wave absorption Adjustable pore structure Polarization loss Attenuation mechanism

Enhancing electromagnetic wave absorption in carbon fiber using FeS_(2)nanoparticles

在线全文

学校读者我要写书评

暂无评论

Nano Research 2023年第7期16卷 9591-9601页

作者： Yuying Guo Meng Zhang tingting Cheng Yuxin Xie Laibin Zhao Liang Jiang Wenxin Zhao Liying Yuan Alan Meng Jian Zhang Ting Wang Zhenjiang Li College of Electromechanical Engineering Key Laboratory of Polymer Material Advanced Manufacturing’s Technology of Shandong provinceCollege of Materials Science and EngineeringQingdao University of Science and TechnologyQingdao 266061China College of Chemical Engineering Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life ScienceMOEShandong Key Laboratory of Biochemical AnalysisCollege of Chemistry and Molecular EngineeringQingdao University of Science and TechnologyQingdao 266042China College of Textiles and Clothing Qingdao UniversityQingdao 266071China

Carbon-based electromagnetic wave absorbing materials(absorbers)adhered with metallic sulfide nanoparticles of good electrical conductivity attract increasing researchers’attention.In this study,on the basis of carbon fiber(C_(f))@Fe_(3)O_(4) nanocomposites obtained by the electrostatic spinning and reflow method,C_(f)@FeS_(2)nanocomposite was successfully prepared during a further hydrothermal process.The products exhibit excellent electromagnetic wave absorption performances with a minimum reflection loss(RLmin)of-54.11 dB at 2.13 mm matching thickness.At the same time,the optimal effective absorption bandwidth(EAB)value of 6.04 GHz at a thickness of 1.98 mm covers the whole ku band,suggesting its excellent electromagnetic wave absorption performances.In addition,the interlaced network structure constructed by carbon fiber,outstanding conductivity of FeS_(2)nanoparticles,and interfacial polarization from hetero-structure play significant parts in enhancing the electromagnetic parameters and absorption performances.All these results suggest that the C_(f)@FeS_(2)nanocomposites can be taken as a new electromagnetic wave-absorbing material under their low density,simple craft,and strong absorption characteristics.

关键词： constructed network structure carbon fiber(C_(f))@FeS_(2)nanocomposite enhanced dielectric loss microwave attenuation mechanism