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安农1687抗条锈病候选基因TraesCS2A01G070700鉴定与分析

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华北农学报 2024年第1期39卷 150-155页

作者：李凯丽耿明状王胜郝维浩卢杰陈璨司红起安徽农业大学农学院安徽合肥230036 安徽省农业科学院水稻研究所安徽合肥230036

为了探索安农1687对于条锈病抗性的遗传机理,加快安农1687小麦品种的推广,为小麦新品种的遗传改良提供参考,减少小麦条锈病对于我国小麦生产安全的危害。以安农1687(安农1687是由安农1106和西农822杂交选育的,对生理小种CYR32具有抗性... 详细信息

为了探索安农1687对于条锈病抗性的遗传机理,加快安农1687小麦品种的推广,为小麦新品种的遗传改良提供参考,减少小麦条锈病对于我国小麦生产安全的危害。以安农1687(安农1687是由安农1106和西农822杂交选育的,对生理小种CYR32具有抗性的半冬性小麦品种)及其姊妹系和亲本为材料,利用小麦55K SNP芯片对安农1687及其双亲和姊妹系进行全基因组扫描,同时利用生理小种CYR32对安农1687及其姊妹系进行接种和鉴定,并在成株期统计其抗条锈病等级,综合接种及田间表型对小麦品系抗条锈病进行评价。鉴定结果显示,安农1687和5个姊妹系(系24、系26、系28、系30、系140)为中抗条锈病,2个姊妹系(系89和系105)为中感条锈病。利用安农1687及其姊妹系间的基因组差异信息,发现差异SNP位点富集在2A染色体短臂的31~37 Mb区间上,说明2A染色体可能存在某个或某些基因导致了这样的结果。为了排除其他抗条锈病基因的干扰,对亲本及2A染色体上携带的已知抗条锈病基因进行验证,结果表明,安农1687的2A染色体短臂的31~37 Mb区间可能存在一个新抗条锈病基因。通过试验和生物信息学分析发现,该区段内的TraesCS2A01G070700基因在抗感品系的NBS结构域上存在差异,感病品系中NBS结构域有3个错义突变,推测TraesCS2A01G070700为安农1687抗条锈病候选基因。

关键词：小麦条锈病姊妹系 SNP

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ABO blood group is a predictor of survival in patients with laryngeal cancer

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Chinese Journal of Cancer 2016年第12期35卷 706-712页

作者： ting Jin Pei-Jing Li Xiao-Zhong chen Wei-Han Hu Key Laboratory of Radiation Oncology in Zhejiang Province Hang-zhou 310022ZhejiangP.R.China. Department of Radiation Oncology Zhejiang Cancer Hospital38 Guang Ji RoadHangzhou 310022ZhejiangP.R.China. State Key Laboratory of Oncology in South China Department of Radiation OncologyCollaborative Innovation Center of Cancer MedicineSun Yat-sen University Cancer CenterNo.651 Dongfeng Road EastGuang-zhou 510060GuangdongP.R.China.

Background: Whether the ABO blood group is associated with the survival of patients with laryngeal cancer remains unknown. The purpose of this study was to investigate the association between the ABO blood group and clinico?pathologic characteristics of patients with laryngeal cancer and assess whether the ABO blood group was associated with ***: We analyzed the records of 1260 patients with laryngeal cancer who underwent curative treatment at Sun Yat?sen University Cancer Center between January 1993 and December 2009. The Chi?square test was used to assess the relationship between the ABO blood group and clinicopathologic characteristics. The Kaplan–Meier method was used to estimate 3?, 5?, and 10?year overall survival(OS) rates. The Cox proportional hazards model was used in univariate and multivariate analyses of ***: No signiicant association was found between the ABO blood group and clinicopathologic characteristics except for primary tumor site. The median OS for patients with blood groups A, B, AB, and O were 87.0, 80.0, 90.0, and 72.5 months, respectively. The 3?, 5?, and 10?year OS rates were 82.4%, 76.0%, and 67.5% for patients with blood group A; 77.4%, 69.8%, and 58.4% for patients with blood group B; 82.2%, 73.1%, and 65.6% for patients with blood group AB; and 71.7%, 66.4%, and 55.5% for patients with blood group O, respectively. Univariate and multivariate analyses showed that the ABO blood group had signiicant efects on prognosis in patients with laryngeal ***: The ABO blood group is associated with survival in patients with laryngeal cancer. Patients with blood group O had signiicantly shorter OS than patients with other ABO blood groups.

关键词： The ABO blood group Laryngeal cancer Prognosis Survival

红螯螯虾不同养殖群体和选育群体遗传多样性及遗传结构分析

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农业生物技术学报 2024年第9期32卷 2112-2123页

作者：欧阳苗峰陈红林刘峰周焕楼宝钱豪杰钱伦湖州师范学院生命科学学院浙江省农业科学院水生生物研究所上海海洋大学水产与生命学院海宁市鸿海养殖有限公司海宁市水产技术服务站

红螯螯虾(Cherax quadricarinatus)是一种世界性养殖的淡水经济虾类，目前在我国许多地区均有养殖。为了解我国红螯螯虾不同地区及人工选育群体遗传多样性和遗传结构，本研究分别利用15对和20对微卫星标记对10个不同地区红螯螯虾养殖群... 详细信息

红螯螯虾(Cherax quadricarinatus)是一种世界性养殖的淡水经济虾类，目前在我国许多地区均有养殖。为了解我国红螯螯虾不同地区及人工选育群体遗传多样性和遗传结构，本研究分别利用15对和20对微卫星标记对10个不同地区红螯螯虾养殖群体和3个世代选育群体进行遗传多样性分析，结果显示，10个养殖群体的平均等位基因数(number of alleles, Na)为5.867～6.867，平均有效等位基因数(effective number of alleles, Ne)为3.090～3.857，香农指数(shannon's diversity index, H')均大于1，平均观测杂合度(observed heterozygosity, Ho)和平均期望杂合度(expected heterozygosity, He)均大于0.5，平均多态信息含量(polymorphism information content, PIC)为0.531～0.595。此外，10个不同地区红螯螯虾养殖群体的基因流(number of migrants per generation, Nm)为11.343～32.526，遗传分化指数(genetic differentiation coefficient, Fst)为0.008～0.027，遗传距离(genetic distance, D)为0.024～0.090，遗传相似系数(genetic similarity index, I)为0.914～0.977。基于D构建的聚类图显示，潮汕与南通、珠海、台湾和平湖群体聚为一个分支，无锡与淮安、海南、诸暨群体聚为一个分支，而汕尾群体单独聚为一个分支。分析3个世代的选育群体发现，其遗传多样性参数发生明显下降，其中，Ho从0.663下降到0.638,H'从1.449下降到1.372,Na从3.509下降到3.303,PIC从0.640下降到0.635，说明通过人工选育导致群体上述各项指标均有所降低。同时，随着选育世代数增加，I从0.961逐渐降低到0.931、D从0.044逐渐升高至0.07，而相邻世代间的I从0.957升高至0.960、Nm从26.262升高至29.895,Fst在渐次变小，其中，选育1代与选育2代间Fst值为0.009，而选育2代与选育3代群体间Fst值降为0.008，进一步说明累代人工选育导致世代间遗传相似性降低，遗传距离增加，世代间的遗传分化逐渐增大。以上研究结果表明，我国不同地区红螯螯虾群体无明显遗传分化，各地区红螯螯虾遗传多样性水平都较高，都可作为良种选育的基础群体；红螯螯虾选育群体经过3代选育后，遗传多样性水平有所降低，但仍处于较高水平，遗传变异与遗传分化水平较低，仍旧具有较强的选育潜力。本研究为红螯螯虾良种选育提供重要的理论依据。

关键词：红螯螯虾养殖群体选育群体遗传多样性遗传结构

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高强海工钢厚板中氧化铝夹杂演变行为模拟

塑性工程学报 2024年第7期31卷 202-211页

作者：张泽飞张兴红刘奕江陈振业魏浩吕知清燕山大学先进锻压成形技术与科学教育部重点实验室河北秦皇岛066004 河钢集团钢研总院河北石家庄050000

为研究轧制工艺对高强海工钢中夹杂物破碎情况的影响规律,对含夹杂物厚板进行了有限元分析,并采用热压缩变形实验对夹杂物演变进行了物理模拟。结果表明:Al_(2)O_(3)夹杂物的破碎是由与基体相接触的表面逐渐传导至心部,并在中间位置发... 详细信息

为研究轧制工艺对高强海工钢中夹杂物破碎情况的影响规律,对含夹杂物厚板进行了有限元分析,并采用热压缩变形实验对夹杂物演变进行了物理模拟。结果表明:Al_(2)O_(3)夹杂物的破碎是由与基体相接触的表面逐渐传导至心部,并在中间位置发生断裂;夹杂物尺寸越大,越容易破碎,当尺寸达到一定值时,尺寸对夹杂物破碎行为的影响会越来越小;相较于相同压下率下的等温轧制,差温轧制厚度方向变形更为均匀,更容易达到心部位置处夹杂物的破碎条件。热变形结果显示,大尺寸(Φ80~Φ100μm)夹杂物发生严重破碎,小尺寸(≤Φ5μm)夹杂物未发生明显破碎,与有限元模拟结果具有一致性。

关键词：差温轧制 Al_(2)O_(3)夹杂物有限元子模型技术内聚力模型

基于CRISPR/Cas9技术的SIRT3基因敲除猪肺泡巨噬细胞系的构建与鉴定

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江西农业大学学报 2024年第4期46卷 991-1000页

作者：王宝鑫张文华董霞郑好张晶陈洪波周傲武汉轻工大学动物科学与营养工程学院/动物遗传繁育与精准养殖实验室湖北武汉430023 湖北省家畜种业技术创新中心湖北武汉430023

【目的】旨在构建稳定敲除SIRT3基因的猪肺泡巨噬细胞系,分析SIRT3敲除对病毒诱导的炎症反应的调控作用,为探究SIRT3基因在宿主抗病毒感染中的作用奠定试验基础。【方法】研究利用CRISPR/Cas9基因编辑技术,针对猪SIRT3基因第2号外显子设... 详细信息

【目的】旨在构建稳定敲除SIRT3基因的猪肺泡巨噬细胞系,分析SIRT3敲除对病毒诱导的炎症反应的调控作用,为探究SIRT3基因在宿主抗病毒感染中的作用奠定试验基础。【方法】研究利用CRISPR/Cas9基因编辑技术,针对猪SIRT3基因第2号外显子设计sgRNA,连接pb-U6-puro-BFP质粒后转染3D4/21细胞,筛选单克隆细胞,结合Sanger测序、qPCR和Western blot技术对获得的单克隆细胞进行鉴定,进而获得SIRT3基因敲除的猪肺泡巨噬细胞系(3D4/21-SIRT3-KO),并以流感病毒A/WSN/33为研究对象,感染野生型和3D4/21-SIRT3-KO猪肺泡巨噬细胞,利用qPCR方法检测炎症相关因子IL-6、IL-8的表达水平,初步分析SIRT3基因在流感病毒感染诱导炎症反应的影响。【结果】Sanger测序结果显示,在挑选获得的敲除SIRT3基因的猪肺泡巨噬细胞克隆中,SIRT3基因第2号外显子位点产生了碱基缺失,并导致移码突变;同时,利用qPCR和Western blot检测猪肺泡巨噬细胞中SIRT3 mRNA和蛋白水平的表达,结果显示与野生型细胞相比,3D4/21-SIRT3-KO细胞中SIRT3 mRNA和SIRT3蛋白均不表达;流感病毒感染SIRT3基因敲除的猪肺泡巨噬细胞时发现,敲除SIRT3能显著加剧流感病毒感染诱导的炎症反应。【结论】研究利用CRISPR/Cas9基因编辑技术成功构建了SIRT3基因敲除的猪肺泡巨噬细胞系,并初步分析了SIRT3在流感病毒感染中作用。

关键词：猪肺泡巨噬细胞系 SIRT3 CRISPR/Cas9 炎症反应抗病育种基因编辑

Using Statistical Learning Algorithms in Regional Landslide Susceptibility Zonation with Limited Landslide Field Data

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Journal of Mountain Science 2015年第2期12卷 268-288页

作者： WANG Yi-ting SEIJMONSBERGEN Arie Christoffel BOUTEN Willem chen Qing-tao State Key Laboratory of Remote Sensing Science School of Geography Beijing Normal University National Marine Data & Information Service Institute for Biodiversity and Ecosystem Dynamics University of Amsterdam Institute of Remote Sensing and GIS Chengdu University of Technology

Regional Landslide Susceptibility Zonation(LSZ) is always challenged by the available amount of field data, especially in southwestern China where large mountainous areas and limited field information coincide. Statistical learning algorithms are believed to be superior to traditional statistical algorithms for their data adaptability. The aim of the paper is to evaluate how statistical learning algorithms perform on regional LSZ with limited field data. The focus is on three statistical learning algorithms, Logistic Regression(LR), Artificial Neural Networks(ANN) and Support Vector Machine(SVM). Hanzhong city, a landslide prone area in southwestern China is taken as a study case. Nine environmental factors are selected as inputs. The accuracies of the resulting LSZ maps are evaluated through landslide density analysis(LDA), receiver operating characteristic(ROC) curves and Kappa index statistics. The dependence of the algorithm on the size of field samples is examined by varying the sizes of the training set. The SVM has proven to be the most accurate and the most stable algorithm at small training set sizes and on all known landslide sizes. The accuracy of SVM shows a steadilyincreasing trend and reaches a high level at a small size of the training set, while accuracies of LR and ANN algorithms show distinct fluctuations. The geomorphological interpretations confirm the strength of SVM on all landslide sizes. Our results show that the strengths of SVM in generalization capability and model robustness make it an appropriate and efficient tool for regional LSZ with limited landslide field samples.

关键词： Landslide Susceptibility Zonation(LSZ) Logistic Regression(LR) Artificial Neural Network(ANN) Support Vector Machine(SVM) Regional scale Southwest China

利用均匀密度等离子体通道加速质子的辐射压力增强方案

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物理学报 2024年第11期73卷 218-230页

作者：杨露王晓南陈鑫陈鹏帆夏倩雯熊力龙昊雨李林洋毛小保周海龙张玮炜兰小飞何阳帆西华师范大学物理与天文学院南充637001 攀枝花第三高级中学校攀枝花617099

提出了辐射压力加速与激光尾波场加速相结合的高能质子加速方案.该方案中在临界密度薄靶后端增加一个均匀密度等离子体通道获得了峰值能量为22.2 GeV、截止能量为36.4 GeV和电荷量为0.67 nC的高能质子束,与质子在纯辐射压力加速中相比,... 详细信息

提出了辐射压力加速与激光尾波场加速相结合的高能质子加速方案.该方案中在临界密度薄靶后端增加一个均匀密度等离子体通道获得了峰值能量为22.2 GeV、截止能量为36.4 GeV和电荷量为0.67 nC的高能质子束,与质子在纯辐射压力加速中相比,其高能质子的截止能量可以提升2个数量级.结果证实了通过在近临界密度薄靶后面连接一个均匀密度等离子体通道,等离子体通道中激光激发的尾波场可以捕获经辐射压力过程预加速的质子并维持长时间的加速,最终获得高能质子,本文还研究了不同均匀密度等离子体通道中质子的加速情况,发现密度越高,则被加速质子的峰值能量和截止能量越高,电荷量也越大.该组合加速方案对高能质子束的产生与应用具有一定的指导意义.

关键词：辐射压力加速激光尾波场加速高能质子束等离子体通道

Robustly Tuning Bandgaps in Two-Dimensional Soft Phononic Crystals with Criss-Crossed Elliptical Holes

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Acta Mechanica Solida Sinica 2018年第5期31卷 573-588页

作者： Nan Gao Yi-lan Huang Rong-hao Bao Wei-qiu chen Department of Engineering Mechanics Zhejiang University Hangzhou 310027 China State Key Lab of CAD & CG Zhejiang University Hangzhou 310058 China Key Laboratory of Soft Machines and Smart Devices of Zhejiang Province Zhejiang University Hangzhou 310027 China Soft Matter Research Center Zhejiang University Hangzhou 310027 China

Tuning band gaps in soft materials by post-buckling deformation is becoming an appealing means to manipulate elastic waves. As one of the most favorable topologies, two- dimensional soft structures with circular holes have been extensively studied. Based on the contrarian thinking, this paper starts from the two-dimensional soft structures with criss-crossed elliptical holes, which is close to the post-buckling configuration of soft structures with circular holes, and then proposes to tune the band gaps through elongating or stretching rather than compressing. Influences of the loading magnitude and loading pattern （i.e., uniaxial and biaxial elongations） on the band gaps are studied via the nonlinear finite element simulations. Effects of the geometric parameters （the major-to-minor half-axis ratio and the porosity of the structure） are also discussed. It is shown that, compared with the traditional circular hole case, the band gaps of the unloaded structure with criss-crossed elliptical holes are much richer, and they could be reversely and continuously tuned by tensile loadings. In particular, the deformation is very robust and is insensitive to small geometric imperfections, which is always necessary for triggering the post-buckling deformations. The present work provides a useful reference to the manipula- tion of elastic waves in periodic structures as well as the design of soft phononic crystals/acoustic devices.

关键词： Soft phononic crystal Criss-crossed elliptical holes Band gap Tunability

Emerging directions in the study of the ecology and evolution of plant-animal mutualistic networks: a review

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Zoological Research 2015年第2期36卷 65-71页

作者： hao GU Eben GOODALE Jin chen Key Laboratory of Tropical Forest Ecology Xishuangbanna Tropical Botanical Garden Chinese Academy of Sciences Graduate School of Chinese Academy of Sciences

The study of mutualistic plant and animal networks is an emerging field of ecological research. We reviewed progress in this field over the past 30 years. While earlier studies mostly focused on network structure, stability, and biodiversity maintenance, recent studies have investigated the conservation implications of mutualistic networks, specifically the influence of invasive species and how networks respond to habitat loss. Current research has also focused on evolutionary questions including phylogenetic signal in networks, impact of networks on the coevolution of interacting partners, and network influences on the evolution of interacting species. We outline some directions for future research, particularly the evolution of specialization in mutualistic networks, and provide concrete recommendations for environmental managers.

关键词： Mutualistic networks Coevolution Speciation Phylogenetic signal Specialization