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Preparation of （2E）-3-（4＇-Halophenyl）prop-2-enoyl Sulfachlorpyridazine Sodium Salts and Their Interaction with Bovine Serum Albumin by Fluorescence Spectroscopy

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Chemical Research in Chinese Universities 2013年第5期29卷 854-860页

作者： DU Chuan-rong LUO Xuan WEI Jin-rui HE ting-ting ZHENG xiao-yu LIN Cui-wu School of Chemistry and Chemical Engineering Guangxi University Nanning 530004 P. R. China

Three （2E）-3-（4＇-halophenyl）prop-2-enoyl sulfachlorpyridazine sodium salts（XPSCA） were synthesized. Their chemical structures were confirmed by IH NMR and 13C NMR, electrospray ionization mass spectrometry （ESI-MS）, and infrared（IR） spectroscopy. The interactions between XPSCA and bovine serum albumin（BSA） were investigated under imitated physiological condition by fluorescence quenching technique and UV-Vis absorption spectroscopy according to the Stern-Volmer equation. The results from the emission quenching at different tempera- tures indicate that the quenching mechanism of serum albumin by XPSCA was static quenching mechanism at low XPSCA concentrations or a combined quenching（static and dynamic） mechanism at higher XPSCA concentrations. At different temperatures, the binding constant and the binding sites of XPSCA with BSA were investigated, and the distances were evaluated according to F6rster non-radiative resonance energy transfer theory. The thermodynamic parameters were calculated according to van＇t Hoff equation, which implies that both van der Waals interaction and hydrogen bond played major roles in stabilizing the XPSCA-BSA complexes, whereas hydrophobic interactions were secondary. Moreover, the conformational changes in BSA were analyzed by synchronous fluorescence spectra.

关键词： Sulfachlorpyridazine sodium Bovine serum albumin Halo-cinnamic acid Fluorescence spectroscopy

Meta-analysis of risk factors associated with atherosclerosis in patients with Kawasaki disease

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World Journal of Pediatrics 2016年第3期12卷 308-313页

作者： Han Zhang Ming-Guo Xu Li-Jian Xie Min Huang Jie Shen ting-ting xiao Department of Cardiology Shanghai Children's HospitalShanghai Jiaotong UniversityShanghaiChina Department of Cardiovascular Center Shenzhen Children's HospitalShenzhenChina

Background:Kawasaki disease(KD)has now become the leading cause of acquired heart disease among children in developed *** study investigated whether patients with KD have an increased risk of ***:Electronic databases,including PubMed,Embase and Springer link,were searched through June 1,2015,for eligible *** were included when they met the following criteria:1)an observational study focusing on evaluating the risk factors for atherosclerosis in patients with KD;2)KD was diagnosed clinically according to the Japan Kawasaki Disease Research Committee or American Heart Association's diagnostic criteria;3)the study subjects were KD patients without coronary heart disease or related cardiovascular disease(KD group)and non-KD patients as control(control group),and 4)investigation of important atherosclerosis risk factors,total cholesterol(TC),low-density lipoprotein cholesterol(LDL),triglycerides(TG),systolic blood pressure(SBP),and fl owmediated dilatation(FMD).The methodological quality of the included studies was evaluated using the Newcastle-Ottawa *** difference(MD)and relative risk(RR)and corresponding 95%confidence intervals(CI)were used to calculate the pooled ***:Sixteen studies were included with a total of 870 patients,including 421 KD patients and 449 non-KD *** in TG and SBP between KD patients and controls were not signifi cant;in contrast,TC and LDL levels were significantly higher in KD patients than the controls,whereas FMD in the KD patients was signifi cantly ***:KD patients may have an increased risk of developing atherosclerosis.

关键词： atherosclerosis fl ow-mediated dilatation Kawasaki disease meta-analysis total cholesterol

Installing the neurospora carotenoid pathway in plants enables cytosolic formation of provitamin A and its sequestration in lipid droplets

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Molecular Plant 2023年第6期16卷 1066-1081页

作者： Xiongjie Zheng Yasha Zhang Aparna Balakrishna Kit Xi Liew Hendrik N.J.Kuijer ting ting xiao Ikram Blilou Salim Al-Babili The BioActives Lab Center for Desert Agriculture(CDA)Biological and Environmental Science and Engineering(BESE)King Abdullah University of Science and Technology(KAUST)Thuwal 23955-6900Saudi Arabia The Laboratory of Plant Cell and Developmental Biology(LPCDB) Center for Desert Agriculture(CDA)Biological and Environmental Science and Engineering(BESE)King Abdullah University of Science and Technology(KAUST)Thuwal 23955-6900Saudi Arabia Present address:College of Plant Science and Technology Bejing Key Laboratory for Agricultural Application and New TechniqueBeijing University of AgricultureBeijing 102206China

Vitamin A deficiency remains a severe global health issue,which creates a need to biofortify crops with provitamin A carotenoids(PACs).Expanding plant cell capacity for synthesis and storing of PACs outside the plastids is a promising biofortification strategy that has been little ***,we engineered PAC formation and sequestration in the cytosol of Nicotiana benthamiana leaves,Arabidopsis seeds,and citrus callus cells,using a fungal(Neurospora crassa)carotenoid pathway that consists of only three enzymes converting C5 isopentenyl building blocks formed from mevalonic acid into PACs,including β-*** strategy led to the accumulation of significant amounts of phytoene and γ-and β-carotene,in addition to fungal,health-promoting carotenes with 13 conjugated double bonds,such as the PAC torulene,in the *** the isopentenyl diphosphate pool by adding a truncated Arabidopsis hydroxymethylglutaryl-coenzyme A reductase substantially increased cytosolic carotene *** carotenes accumulate in cytosolic lipid droplets(CLDs),which represent a novel sequestering sink for storing these pigments in plant ***,β-carotene accumulated in the cytosol of citrus callus cells was more light stable compared to compared with plastidialβ-***,engineering cytosolic carotene formation increased the number of large-sized CLDs and the levels of β-apocarotenoids,including retinal,the aldehyde corresponding to vitamin ***,our study opens up the possibility of exploiting the high-flux mevalonic acid pathway for PAC biosynthesis and enhancing carotenoid sink capacity in green and non-green plant tissues,especially in lipid-storing seeds,and thus paves the way for further optimization of carotenoid biofortification in crops.

关键词： Neurospora crassa carotenoid biosynthesis provitamin A metabolic engineering synthetic metabolism cytosolic lipid droplets carotenoid sequestration carotenoid stability

Successful Agrobacterium-mediated transformation of Populus tomentosa with apple SPDS gene

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Forestry Studies in China 2008年第3期10卷 153-157页

作者： LIU ting-ting PANG xiao-ming LONG Cui ZHANG Zhi-yi Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education Beijing Forestry University Beijing 100083 P. R. China

The problem of salinized soils has become one of the most serious constraints to agricultural and forest productivity. With the purpose of enhancing salt stress tolerance of Populus tomentosa, we transformed this tree species with spermidine synthase （SPDS） genes derived from an apple by an Agrobacterium-mediated method. Four transgenic clones were confu＇med by PCR and Southern blot analysis. As well, the expression of introduced SPDS genes was analyzed by real-time quantitative PCR.

关键词： salt tolerance spermidine synthase gene transformation Populus tomentosa

A Deep-Ecological Reading of William Wordsworth＇s Poetry

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Sino-US English Teaching 2014年第3期11卷 188-195页

作者： YANG ting-ting xiao Bin Beijing Information Science & Technology University Beijing China

From the 1960s, the increasingly serious ecological crisis gave rise to ecocriticism. William Wordsworth （1770-1850）, a representative of romantic poets, composes substantial poems to eulogize nature and to encourage equality and harmony between nature and human beings. From the angle of deep ecology, a branch of ecological study, this paper aims to analyze the ecological consciousness conveyed in Wordsworth＇s poetry. It analyzes the adverse impacts of industrialization on nature and man in Wordsworth＇s poetry. It elaborates further on ecological thoughts in Wordsworth＇s poetry. And finally, it summarizes how Wordsworth criticizes industrialization in his poetry and how he expresses his ecological thoughts--ecological equilibrium, social equality and harmony, and nature preservation

关键词： deep ecology ecological equilibrium social equality nature preservation

Down-regulation of peroxisome proliferator-activated receptor γ coactivator-1α expression in fatty acid-induced pancreatic betacell apoptosis involves nuclear factor-κB pathway

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Chinese Medical Journal 2011年第22期124卷 3657-3663页

作者： HE ting-ting CAO xiao-pei CHEN Ru-zhu ZHU xiao-nan WANG Xue-lan LI Yan-bing xiao Hai-peng Department of Endocrinology First Affiliated Hospital of Sun Yat-sen University Guangzhou Guangdong 510080 China

Background Pancreatic beta-cell apoptosis induced by lipotoxicity, to a large extent, contributes to the progression of type 2 diabetes. To investigate the mechanism of free fatty acid induced apoptosis, we aimed to study the effects of palmitic acid （PA） on the apoptosis and peroxisome proliferator-activated receptor y coactivator-1α （PGC-1α） expression in βTC3 cells as well as the possible role of nuclear factor-KB （NF-KB） in this process. Methods Hoechst 33258 was used to detect βTC3 cell apoptosis, which was induced by PA stimulation for 12 hours. PGC-1α expression was analyzed by reverse transcription polymerase chain reaction, IκB kinase β （IKKβ）, IκBα NF-KB-inducing kinase （NIK） and ReI-B expressions were analyzed by Western blotting. MGβ2 was employed to block the endogenous IκBαdegradation before PA administration, and then its effect on PA-inducing cell apoptosis and PGC-1α mRNA expression was analyzed. Results Significant increased cell apoptosis was found at the concentration of 0.5 mmol/L and 1.0 mmol/L PA administration. PA （0.5 mmol/L） could extensively reduced the expression of PGC-1α mRNA. After exposing βTC3 cells to 0.5 mmol/L PA for different time periods （0, 4, 6, 8, 10 and 12 hours）, IKKβ protein expression increased while IκBα NIK and ReI-B protein expression declined in a time-dependent manner. Pretreatment with MGβ2 to inhibit the degradation of IκBα partially prevented the down-regulation of PGC-1α mRNA expression after 12-hour PA treatment in accordance with the decrease of PA induced apoptosis. Conclusions NF-KB canonical pathway was activated in PA-mediated βTC3 cell apoptosis, whereas non-canonical pathway was inhibited. Reduced PGC-1α expression by PA in βTC3 cells could involve the activation of canonical NF-KB pathway, so as to deteriorate the PA induced apoptosis.

关键词： beta-cell apoptosis peroxisome proliferator-activated receptor 7 coactivator-1α nuclear factor-κB

Generalized Product of Iteration Function System

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Computer Aided Drafting,Design and Manufacturing 2005年第2期15卷 44-49页

作者： ZHAO ting-ting WEI xiao-peng XIA Jun-tian SANG Lin University Key Lab of Information Science ＆ Engineering Dalian University Dalian 116622 China

The definition of generalized product of fractal is first put forward for the research on the relations between original fractal and its product of fractal when the transformations of iteration function system （IFS） are incomplete. Then the representations of generalized product of IFS are discussed based on the theory of the product of fractal. Furthermore, the dimensional relations between the product of fractal and its semi-product are obtained. The dimensional relations of self-similar set are discussed. Finally, the examples for rendering fractal graphs are given. These results posses potentials in image compression and pattern recognition.

关键词： fractal iteration function system product of fractal semi-product of fractal generalized product of fractal

Long-term outcomes of combined chemotherapy in chronic refractory idiopathic thrombocytopenic purpura

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Chinese Medical Journal 2007年第18期120卷 1643-1646页

作者： TAO Jie HUANG Ying LI Hong-qiang WANG ting-ting WANG xiao-yan JI Lin-xiang YANG Ren-chi State Key Laboratory of Experimental Hematology Institute ofHematology and Hospital of Blood Diseases Chinese Academy ofMedical Sciences and Peking Union Medical College Tianjin300020 China

Adult idiopathic thrombocytopenic purpura （ITP） is a .chronic acquired organ-specific autoimmune hemorrhagic disease characterized by the production of auto-antibodies against antigens on the membranes of platelet, resulting in enhanced Fc-mediated destruction of the platelets by macrophages in the reticuloendothelial 1-3 system. Splenectomy remains the best treatment of chronic ITP, but approximately 30% of patients do not respond or relapse after surgery;4-6 these patients are defined as patients with chronic refractory ITE There is no consensus on an appropriate sequence of treatments for patients with chronic refractory ITE79 Management of these patients remains a dilemma. We have treated 31 patients with chronic refractory ITP using low dose combination chemotherapy between 1994 and 2005, and the results are presented as follows.

关键词： purpura, idiopathic drug therapy, combination outcome assessment, health care

Atomically precise nickel-sulfur clusters for efficient electrochemical 2,5-hydroxymethylfurfural oxidation

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Nano Research 2025年第1期18卷 140-148页

作者： Fen Wang Lu-Yu Liu Sha-Sha Cui ting-ting Li xiao-Meng Yang Zhijuan Liu Yanyong Wang Henan Key Laboratory of Crystalline Molecular Functional Materials Henan International Joint Laboratory of Tumor Theranostical Cluster MaterialsGreen Catalysis Centerand College of ChemistryZhengzhou UniversityZhengzhou 450001China State Key Laboratory of Chem/Bio-sensing and Chemometrics College of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082China

Nickel sulfide exhibits excellent catalytic activity in the electrochemical 2,5-hydroxymethylfurfural oxidation reaction(HMFOR).However,due to the polydispersity of nanoparticles,it is difficult to establish a clear structure-activity relationship at the atomic *** this work,we have successfully synthesized atomically precise Ni_(6)(PET)_(12)and Ni_(4)(PET)_(8)clusters(PET:2-phenylethanethiol)for ***^(2+)and S_(2)-with atomic ratio of 1:2 was mainly existed in Ni_(6)(PET)_(12)and Ni_(4)(PET)_(8)to form Ni-S *** electrochemical test results have suggested both Ni_(6)(PET)_(12)and Ni_(4)(PET)_(8)displayed outstanding electrocatalytic ability for *** Ni_(6)(PET)_(12)exhibited better electrocatalytic ability than Ni_(4)(PET)_(8)with higher current density,lower overpotential and faster reaction *** superior electrochemical ability of Ni_(6)(PET)_(12)may be due to the enhanced adsorption towards HMF molecule with strong interaction towards hydroxyl group and furan ***,it found that the Ni^(2+)species in Ni_(6)(PET)_(12)could rapidly oxidized into Ni^(3+)species,which could spontaneously capture electron and proton from HMF for *** theoretical calculation demonstrated that the Ni_(6)(PET)_(12)process lower free energy barrier than Ni_(4)(PET)_(8)to display excellent electrocatalytic *** work is of great significance for designing efficient electrocatalysts for HMFOR.

关键词： atomically precise nickel nanoclusters 2 5-hydroxymethylfurfural electro-oxidation Ni3+species