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Effect of active ingredient of Stephaniajaponica(Thunb.) Mierson lipopolysaccharide-induced activation of microglia

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中国药理学与毒理学杂志 2019年第10期 844-845页

作者： hao ting-yu YANG Yan-qiu XU Ji-kai LIU Jing-yu CHE Yue LI Ning HOU Yue College of Life and Health Sciences Northeastern University School of Traditional Chinese Materia Medica Key Laboratory of StructureBased Drug Design and Discovery Shenyang Pharmaceutical University

OBJECTIVE Microglia were considered to be the main immune cells in the central nervous system, which could maintain the homeostasis of the *** pathological conditions, microglia undergo significant changes in morphology and release a large number of pro-inflammatory mediators. Numerous studies have shown that neuroinflammatory responses mediated by microglial over-activation are involved in the pathogenesis of multiple neurodegenerative diseases. Therefore, finding a safe and effective drug that can inhibit microglialoveractivation becomes one of the significant strategies to prevent and treat neurodegenerative diseases. In our previous studies, we found that the dichloromethane（CH;Cl;） fraction of the 80% EtOH extraction of Stephaniajaponica, could inhibit nitric oxide（NO） release in microglial cel line induced by LPS. The compound QJT-14,obtained from the fraction, exhibited good activity. The purpose of this study is to explore the potential mechanism of QJT-14 suppressing microglia *** Cell viability and NO production were detected by MTT and Griess assay, respectively. The mRNA expression of IL-1β, IL-6, TNF-α were measured by real-time PCR. The production of IL-6, TNF-α were tested by *** protein expression of NF-κB P65 were tested by Western blotting. NF-κB P65 nuclear translocation was detected by immunofluorescence assay. RESULTS JT-14 could significantly suppress NO production in LPSinduced microglia cell line, and did not affect the cell viability within the concentration range. QJT-14 attenuated pro-inflammatory factors expression at m RNA and protein levels in LPS-induced microglia. QJT-14 could significantly inhibit NF-κB P65 protein expression induced by *** QJT-14 could inhibit microglia activation by inhibiting NF-κB pathway. Thesere sults indicated that QJT-14 has a potential therapeutic effect on neurodegenerative diseases.

关键词： Stephaniajaponica neuroinflammation microglia NF-κB

Effects of oxygen concentration and irradiation defects on the oxidation corrosion of body-centered-cubic iron surfaces:A first-principles study

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Chinese Physics B 2022年第8期31卷 566-577页

作者： Zhiqiang Ye Yawei Lei Jingdan Zhang Yange Zhang Xiangyan Li Yichun Xu Xuebang Wu C.S.Liu ting hao Zhiguang Wang Key Laboratory of Materials Physics Institute of Solid State PhysicsHFIPSChinese Academy of SciencesHefei 230031China University of Science and Technology of China Hefei 230026China School of Mechanical Engineering Suzhou University of Science and TechnologySuzhou 215009China Institute of Modern physics Chinese Academy of SciencesLanzhou 730000China

Oxidation corrosion of steels usually occurs in contact with the oxygen-contained environment, which is accelerated by high oxygen concentration and irradiation. The oxidation mechanism of steels is investigated by the adsorption/solution of oxygen atoms on/under body-centered-cubic(bcc) iron surfaces, and diffusion of oxygen atoms on the surface and in the near-surface region. Energetic results indicate that oxygen atoms prefer to adsorb at hollow and long-bridge positions on the Fe(100) and(110) surfaces, respectively. As the coverage of oxygen atoms increases, oxygen atoms would repel each other and gradually dissolve in the near-surface and bulk region. As vacancies exist, oxygen atoms are attracted by vacancies, especially in the near-surface and bulk region. Dynamic results indicate that the diffusion of O atoms on surfaces is easier than that into near-surface, which is affected by oxygen coverage and vacancies. Moreover, the effects of oxygen concentration and irradiation on oxygen density in the near-surface and bulk region are estimated by the Mc Lean’s model with a simple hypothesis.

关键词： oxidation corrosion oxygen concentration irradiation defects bcc-Fe surfaces first-principles study

Genetic diversity and phylogenetic analysis of EG95 sequences of Echinococcus granulosus:Implications for EG95 vaccine application

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Asian Pacific Journal of Tropical Medicine 2017年第5期10卷 497-500页

作者： Wei Pan De-Sheng Chen Yun-Juan Lu Hui-Wen Xu Wen-ting hao Ya-Wen Zhang Su-Ping Qin Kui-Yang Zheng Ren-Xian Tang Jiangsu Key Laboratory of Immunity and Metabolism Department of Pathogenic Biology and ImmunologyLaboratory of Infection and ImmunityXuzhou Medical University Department of Clinical Medicine Xuzhou Medical University

Objective:To analyse the genetic variability of EG95 sequences and provide guidance for EG95 vaccine application against Echinococcus granulosus(E. granulosus). Methods:We analysed EG95 polymorphism by collecting total 97 different E. granulosus isolates from 12 different host species that originated from 10 different countries. Multiple sequence alignments and the homology were performed by Lasergene 1(DNASTAR Inc.,Madison,WI),and the phylogenetic analysis was performed by using MEGA5.1(CEMI,Tempe,AZ,USA). In addition,linear and conformational epitopes were analysed,including secondary structure,NXT/S glycosylation,fibronectin type ecoⅢ(Fnndary Ⅲ) domain and glycosylphosphatidylinositol anchor signal(GPIanchor). The s structure was predicted by PSIPRED method. Results:Our results indicated that most isolates overall shared 72.6-100% identity in EG95 gene sequence with the published standard EG95 sequence,X90928. However,EG95 gene indeed has polymorphism in different isolates. Phylogenetic analysis showed that different isolates could be divided into three subgroups. Subgroup 1 contained 87 isolates while Subgroup 2 and Subgroup 3 consisted of 3 and 7 isolates,respectively. Four sequences cloned from oncosphere shared a high identity with the parental sequence of the current vaccine,X90928,and they belonged to Subgroup 1. However,in comparison to X90928,several amino acid mutations occurred in most isolates besides oncosphere,which potentially altered the immunodominant linear epitopes,glycosylation sites and secondary structures in EG95 genes. All these variations might change their previous antigenicity and thereby affecting the efficacy of current EG95 vaccine. Conclusions:This study reveals the genetic variability of EG95 sequences in different E. granulosus isolates,and proposed that more vaccination trials would be needed to test the effectiveness of current EG95 vaccine against distinct isolates in different countries.

关键词： Echinococcus granulosus Cystic echinococcosis EG95 Vaccine Genetic diversity

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基于广义位势理论的接触面弹塑性本构模型

岩土工程学报 2008年第10期30卷 1532-1536页

作者：周爱兆卢廷浩岩土力学与堤坝工程教育部重点实验室江苏南京210098 河海大学岩土工程科学研究所江苏南京210098

基于广义位势理论,把土与结构的接触面问题看作(σn,τ)空间上的二维问题,用两个线性无关的势函数梯度矢量表示两个塑性应变增量的分量dεnp,dγp组成的矢量,并用塑性状态方程取代传统的屈服面,建立了双重势面接触面弹塑性新模型。结合... 详细信息

基于广义位势理论,把土与结构的接触面问题看作(σn,τ)空间上的二维问题,用两个线性无关的势函数梯度矢量表示两个塑性应变增量的分量dεnp,dγp组成的矢量,并用塑性状态方程取代传统的屈服面,建立了双重势面接触面弹塑性新模型。结合含砾粗砂与钢板接触面单剪试验以及单向压缩试验资料,说明模型参数的确定方法,其中采用抛物线和一阶指数衰减曲线拟合接触面先剪缩后剪胀的试验曲线,曲线参数有物理意义且拟合精度高,参数完全可以通过试验确定。结果表明,模型数学原理清晰,物理假设少,且具有一般性,能够反映接触面的剪胀特性以及准确模拟接触面的变形特性。

关键词：广义位势理论塑性状态方程接触面本构模型

Systems-Based Interactome Analysis for Hematopoiesis Effect of Angelicae sinensis Radix: Regulated Network of Cell Proliferation towards Hemopoiesis

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Chinese Journal of Integrative Medicine 2019年第12期25卷 939-947页

作者： ZHENG Guang ZHANG He YANG Yun SUN Ying-li ZHANG Yan-jing CHEN Ju-ping hao ting LU Cheng GUO Hong-tao ZHANG Ge FAN Dan-ping HE Xiao-juan LU Ai-ping Information Science and Engineering School Lanzhou UniversityLanzhou(730000)China School of Biology Lanzhou UniversityLanzhou(730000)China Institute of Basic Research in Chinese Medicine China Academy of Chinese Medical SciencesBeijing(100700)China Department of Rheumatism the First Affiliated Hospital of Henan University of Traditional Chinese MedicineZhengzhou(450000)China School of Chinese Medicine Hong Kong Baptist UniversityKowloon TongKowloonHong Kong SARChina

Objective: To explore the molecular-level mechanism on the hematopoiesis effect of Angelicae sinensis Radix(ASR) with systems-based interactome analysis. Methods: This systems-based interactome analysis was designed to enforce the workflow of "ASR(herb)→compound→target protein→internal protein actions→ending regulated protein for hematopoiesis". This workflow was deployed with restrictions on regulated proteins expresses in bone marrow and anemia disease and futher validated with experiments. Results: The hematopoiesis mechanism of ASR might be accomplished through regulating pathways of cell proliferation towards hemopoiesis with cross-talking agents of spleen tyrosine kinase(SYK), Janus kinase 2(JAK2), and interleukin-2-inducible T-cell kinase(ITK). The hematopoietic function of ASR was also validated by colonyforming assay performed on mice bone marrow cells. As a result, SYK, JAK2 and ITK were activated. Conclusion: This study provides a new approach to systematically study and predict the therapeutic mechanism for ASR based on interactome analysis towards biological process with experimental validations.

关键词： Angelicae sinensis Radix interactome cell proliferation hemopoiesis regulation cross-talking agents

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论陇东窑洞的文化艺术价值

兰州大学学报（社会科学版） 2005年第3期33卷 147-150页

作者：徐浩艇陇东学院美术系甘肃西峰745000

陇东窑洞是原始洞穴居住形式的延续和发展,以“陶复”、“陶穴”为主要类型。陇东窑洞的经济实用,体现了朴素自然的美学意义,涉及到民俗文化、周易文化、天人合一。面对趋向消亡的陇东窑洞,必须做好能体现陇东文化特点的窑洞的修缮和保... 详细信息

陇东窑洞是原始洞穴居住形式的延续和发展,以“陶复”、“陶穴”为主要类型。陇东窑洞的经济实用,体现了朴素自然的美学意义,涉及到民俗文化、周易文化、天人合一。面对趋向消亡的陇东窑洞,必须做好能体现陇东文化特点的窑洞的修缮和保护工作。

关键词：陇东窑洞原始居住形式

研发投入、纯技术效率与企业价值研究——来自中国医药制造业上市公司的经验证据

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中国科技论坛 2016年第2期 60-66页

作者：郝婷赵息天津大学管理与经济学部天津300072 内蒙古科技大学经济与管理学院内蒙古包头014010

本文采用2009—2013年沪深A股上市的132家医药制造业公司411个混合截面数据,对研发投入、纯技术效率与企业价值之间的关系进行实证检验。通过相关分析和层次回归后的实证结果显示:研发投入以及纯技术效率对企业价值均产生正向影响;研发... 详细信息

本文采用2009—2013年沪深A股上市的132家医药制造业公司411个混合截面数据,对研发投入、纯技术效率与企业价值之间的关系进行实证检验。通过相关分析和层次回归后的实证结果显示:研发投入以及纯技术效率对企业价值均产生正向影响;研发投入对企业价值的影响具有滞后效应,滞后期为一期;纯技术效率对研发投入与企业价值之间的关系产生正向调节作用。

关键词：研发投入企业价值纯技术效率层次回归

Mendelian randomization analysis:the causal relationship between statins and eye diseases

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Medical Data Mining 2024年第4期7卷 34-40页

作者： Lian-Tai Song Jia-Meng Wei hao-ting Jia Feng-Yi Zhang Qing-Fang Deng Yi-Xiao Wang Huai-Zhi Qin Qian Xu Basic Medical College Chengde Medical UniversityChengde 067000China Department of Biochemistry Chengde Medical UniversityChengde 067000China

Background:The specific role of statins in the field of ophthalmology is not *** have the advantages of pleiotropic,relatively safety and low cost,and are a promising choice for the prevention and management of eye ***,there is a divergence of findings regarding the correlation between statin treatment and ocular ***,our intention is to investigate the impact of statins on eye conditions through the utilization of Mendelian randomization(MR).Methods:The UK Biobank provided data on five statins,while the FinnGen database provided data on six eye diseases,including age-related macular degeneration,glaucoma,diabetic retinopathy,senile cataract,drug-induced cataract,and other *** exploration involved the utilization of various methods including inverse variance weighted(IVW),weighted median,weighted multivariate(weighted mode),and MR-Egger *** assess the reliability of the findings,funnel analysis,MR-Egger regression,leave-one-out method,and Cochran’s Q test were ***,reverse MR analysis was performed to evaluate the potential for reverse causality between statin use and eye ***:Based on IVW analysis,there were three pairs of positive results with significant(P<0.05)causal relationship,including atorvastatin and drug-induced cataract(odds ratio(OR)=1.65E-05,95%confidence interval(CI):2.24E-09–0.12;P_(IVW)=0.02),rosuvastatin and drug-induced cataract(OR=2.77E-18,95%CI:7.53E-35–0.1;P_(IVW)=0.04)and fluvastatin with senile cataract(OR=0.5,95%CI:0.25–0.99;P_(IVW)=0.05).No significant causal relationship was observed between other types of statins and eye *** analysis found that the results were *** MR analysis indicated no evidence of reverse causality between statin use and the examined eye ***:Our study finally verified the strong causal relationship between three drugs and two diseases(atorvastatin and rosuvastatin and drug cataract

关键词： Mendel randomization statins eye diseases

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微反应器内制备三水碳酸镁工艺研究

高校化学工程学报 2018年第5期32卷 1140-1146页

作者：赵畅马学虎兰忠郝婷婷大连理工大学化学工程研究所辽宁省化工资源清洁利用重点实验室辽宁大连116024

基于数值模拟不同形状微通道内的混合特性,以此获得实验流道设计依据,并确定了截面1 mm?1 mm、长度为3000 mm的弯曲微通道反应器。以浓度为0.2 mol?L-1的Na2CO3和MgCl2溶液为原料在微反应器内制备三水碳酸镁晶体,以XRD和SEM为表征手段,... 详细信息

基于数值模拟不同形状微通道内的混合特性,以此获得实验流道设计依据,并确定了截面1 mm?1 mm、长度为3000 mm的弯曲微通道反应器。以浓度为0.2 mol?L-1的Na2CO3和MgCl2溶液为原料在微反应器内制备三水碳酸镁晶体,以XRD和SEM为表征手段,考察物料流量和停留时间对晶体质量的影响。结果表明,物料流量过低或过高都会降低晶核表面积或反应液的过饱和度,进而影响晶体的生长。实验范围内物料流量为4 mL?min-1时产物结晶度高且晶型完整;随着物料在通道内停留时间的增长,产物质量会存在先降低后升高的趋势。在流量4mL?min-1、停留时间9 s条件下得到最佳晶体,晶胞畸变率小,晶棒尺寸分布较均匀(平均粒径为150μm),符合正态分布。

关键词：弯曲通道微反应器数值模拟三水碳酸镁晶型