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Maternal Murine Cytomegalovirus Infection during Pregnancy Up-regulates the Gene Expression of Toll-like Receptor 2 and 4 in Placenta

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Current Medical Science 2018年第4期38卷 632-639页

作者： Yi LIAO Ya-nan ZHANG xing-lou LIU Yuan-yuan LU Lin-lin ZHANG ting xi Sai-nan SHU Feng FANG Department of Pediatrics Tongji Hospital Tongji Medical College Huazhong University of Science and TechnologyWuhan 430032 China

Increasing evidence has revealed that maternal cytomegalovirus （CMV） infection may be associated with neurodevelopmental disorders in offspring. Potential relevance between the placental inflammation and CMV-related autism has been reported by clinical observation. Meanwhile, abnormal expression of Toll-like receptor 2 （TLR2） and TLR4 in placenta of patients with chorioamnionitis was observed in multiple studies. IL-6 and IL- 10 are two important maternal inflammatory mediators involved in neurodevelopmental disorders. To investigate whether murine CMV （MCMV） infection causes alterations in placental IL-6/10 and TLR2/4 levels, we analyzed the dynamic changes in gene expression of TLR2/4 and IL-6/10 in placentas following acute MCMV infection. Mouse model of acute MCMV infection during pregnancy was created, and pre-pregnant MCMV infected, lipopolysaccharide （LPS）-treated and uninfected mice were used as controls. At E13.5, E 14.5 and E 18.5, placentas and fetal brains were harvested and mRNA expression levels of placental TLR2/4 and IL-6/10 were analyzed. The results showed that after acute MCMV infection, the expression levels of placental TLR2/4 and IL-6 were elevated at E13.5, accompanied by obvious placental inflammation and reduction of placenta and fetal brain weights. However, LPS 50 ktg/kg could decrease the IL-6 expression at E13.5 and E14.5. This suggests that acute MCMV infection during pregnancy could up-regulate the gene expression of TLR2/4 in placental trophoblasts and activate them to produce more pro- inflammatory cytokine IL-6. High dose of LPS stimulation （50 gg/kg） during pregnancy can lead to down-regulation of IL-6 levels in the late stage. Imbalance of IL-6 expression in placenta might be associated with the neurodevelopmental disorders in progeny.

关键词： murine cytomegalovirus maternal immune activation placenta TLR2 TLR4 IL-6

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城镇污水厂各工序段对微塑料去除率差异特性分析

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中国环境科学 2024年第6期44卷 3122-3131页

作者：米家辉卢俊平刘廷玺刘禹张琦石震宇刘莹慧内蒙古农业大学水利与土木建筑工程学院内蒙古呼和浩特010018 内蒙古自治区水资源保护与利用重点实验室内蒙古呼和浩特010018

污水处理厂被认为是微塑料的主要排放与储存场所.为探究城镇污水处理厂不同处理工序段对污水中微塑料的去除效率差异特性,以内蒙古某污水处理厂为研究对象,通过采用体式显微镜观察,并借助LAS X软件统计分析的方法展开研究,结果表明:污... 详细信息

污水处理厂被认为是微塑料的主要排放与储存场所.为探究城镇污水处理厂不同处理工序段对污水中微塑料的去除效率差异特性,以内蒙古某污水处理厂为研究对象,通过采用体式显微镜观察,并借助LAS X软件统计分析的方法展开研究,结果表明:污水厂中微塑料的主要存在形态以纤维状为主(61.8%),其次为碎片状与薄膜状,微塑料的尺寸主要集中在0.25-0.50mm(32.9%).一级处理工序中对微塑料去除效果最好的构筑物分别是:粗格栅、细格栅和初沉池,尤其对尺寸小于0.25mm微塑料颗粒的去除效果最好.旋流沉砂池对纤维、碎片和薄膜状微塑料均没有起到积极的去除效果,二级处理工序中的二沉池对微塑料的去除起到了关键作用,其次为纤维生物转盘,其对尺寸介于0.50~1.00mm的微塑料去除效率最高.一级处理工序对3种形状微塑料的去除效率均为正值,且去除效率与微塑料尺寸呈负相关关系.一级处理工序中的构筑物对小尺寸微塑料的去除效率比较稳定,而对大尺寸微塑料的去除效率波动较大,对不同形状微塑料的去除率随污水在各处理构筑物流动方向沿程呈下降趋势.二级处理工序对3种形状微塑料的去除率均呈现为负值,而仅对尺寸小于0.50mm的微塑料去除率为正值.本研究旨在为污水厂提升微塑料去除率的运行管理提供理论指导作用.

关键词：微塑料污水处理厂去除效果赋存形态去除贡献率

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Short-lived AIM2 Inflammasome Activation Relates to Chronic MCMV Infection in BALB/c Mice

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Current Medical Science 2019年第6期39卷 899-905页

作者： Yuan-yuan LU xing-lou LIU Yuan HUANG Yi LIAO ting xi Ya-nan ZHANG Lin-lin ZHANG Sai-nan SHU Feng FANG Department of Pediatrics Tongji Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Absent in melanoma 2(AIM2)inflammasome is a crucial link bridging the innate host defense and the subsequent adaptive immunity when activated by exogenous double stranded DNA(dsDNA).Through establishing models of disseminated murine cytomegalovirus(MCMV)infection in BALB/c and C57BL/6 mice,we evaluated dynamic expression of AIM2 inflammasome components and its relationship with pathological damage and viral replication,trying tofigure out whether AIM2 inflammasome is related to the chronic mechanism of MCMV.BALB/c and C57BL/6 mice were sacrificed on day 0,1,3,7,14 and 28 post infection.Expression levels of AIM2,pro-caspase-1,caspase-1 p20,pro-IL1β and mature IL1β in primary peritoneal macrophages(PMs)and spleens were detected by Western blotting.Contents of IL18 in the serum were detected by ELISA.Pathological examinations of livers were performed,and mRNA levels of MCMV glycoprotein B(gB)in salivary glands also assessed.Results showed that expression levels of AIM2 in PMs and spleens of C57BL/6 mice increased on day 3,even continued to day 28;caspase-1 p20 and mature IL1β increased on day 7,14 and 28;the persistently high expression of IL1β in the serum started on day 1,showing a double peak curve.As for BALB/c mice,expression of AIM2 in PMs increased on day 1 and day 7,while contents of AIM2 in spleens increased on day 1 and day 3;caspase-1 p20 and mature ILip merely increased 7 days fter infection.Thereafter,expression levels of AIM2,caspase-1 p20,mature IL1β and IL18 were limited;the duration of AIM2 inflammasome activation in BALB/c mice was much shorter than that in C57BL/6 mice.The severer pathological damage and more viral replications in BALB/c mice further proved the deficient antiviral immunity to MCMV.In conclusion,the activation of AIM2 inflammasome in BALB/c mice was short-lived,which is quite possibly related to the chronicity of MCMV infection.

关键词： AIM2 murine cytomegalovirus BALB/c mice C57BL/6 mice macrophages

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西北煤电集聚区不同水体水化学特征及氟成因

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中国环境科学 2024年第7期44卷 3810-3822页

作者：韩萱黄磊刘廷玺侯泽明李艳娜许江内蒙古农业大学水利与土木建筑工程学院内蒙古自治区水资源保护与利用重点实验室内蒙古呼和浩特010018 黄河流域内蒙段水资源与水环境综合治理自治区协同创新中心内蒙古呼和浩特010018

为探明西北煤电集聚区不同水体水化学特征以及氟离子分布成因,以鄂尔多斯市补连塔矿区为研究对象,采用Piper三线图、主成分分析、相关性分析等方法,对采集的55个水样进行测试,结合非致癌健康评价模型对地下水中氟离子进行健康风险评估.... 详细信息

为探明西北煤电集聚区不同水体水化学特征以及氟离子分布成因,以鄂尔多斯市补连塔矿区为研究对象,采用Piper三线图、主成分分析、相关性分析等方法,对采集的55个水样进行测试,结合非致癌健康评价模型对地下水中氟离子进行健康风险评估.结果表明:所有水样均呈现出弱碱性;Na^(+)、SO_(4)^(2-)是地表水以及深层地下水的优势离子,浅层地下水的优势离子为Ca2+和HCO_(3)-;地表水的水化学类型主要以SO_(4)·Cl-Na和HCO_(3)·SO_(4)-Na型为主,浅层地下水的化学类型较多,主要以SO_(4)·Cl-Na·Ca、HCO_(3)-Na·Mg、HCO_(3)-Mg和SO_(4)·Cl-Ca型为主,深层地下水则以HCO_(3)·SO_(4)-Ca和SO_(4)-Na型为主;其水化学特征主要受硅酸盐风化溶解作用影响,同时采煤活动促进了不同水体之间水化学转化;地表水、浅层地下水与深层地下水中F变化范围分别在0.16~4.82,0~0.69,0.08~6.26mg/L,地下水中F有19.35%的风险指数超过HQ=1,推测该地区地下水的高氟现象对人类健康产生一定的影响.

关键词：地下水地表水水化学特征主成分分析氟离子

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科尔沁沙地沙丘生态系统水热通量特征及影响驱动因子

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中国环境科学 2024年第2期44卷 1041-1052页

作者：张思敏郝丽娜童新贾天宇张亦然马扩伦硕刘廷玺内蒙古农业大学水利与土木建筑工程学院内蒙古呼和浩特010018 内蒙古自治区水资源保护与利用重点实验室内蒙古呼和浩特010018 黄河流域内蒙段水资源与水环境综合治理协同创新中心内蒙古呼和浩特010018 内蒙古水利水电勘测设计院内蒙古呼和浩特010018

以科尔沁沙地的典型沙丘区为研究对象,利用涡动相关系统(EC)持续监测沙丘生态系统2013~2022年的表面能量通量数据,采用普通最小二乘线性回归法(OLS)和表面能量平衡比率(EBR)两个指标评估能量平衡闭合度,对沙丘生态系统生长季(4~10月)能... 详细信息

以科尔沁沙地的典型沙丘区为研究对象,利用涡动相关系统(EC)持续监测沙丘生态系统2013~2022年的表面能量通量数据,采用普通最小二乘线性回归法(OLS)和表面能量平衡比率(EBR)两个指标评估能量平衡闭合度,对沙丘生态系统生长季(4~10月)能量通量的日变化、季节变化以及年际变化进行分析,并计算波文比(β)和能量平衡比,探讨了影响潜热通量(LE)、感热通量(H)和土壤热通量(G)变化的环境因素.结果表明:多年平均年净辐射(R_(g))为1592.77MJ/m^(2),其中H、LE和G分别为614.60,636.22和117.76MJ/m^(2),科尔沁沙地沙丘生态系统年能量的主要组成部分为潜热通量,占总能量的40%.β日动态曲线呈单峰变化,波动相对较平缓.各年年均表面能量平衡比率(EBR)介于0.75~0.90之间,多年年均EBR为0.87,说明能量平衡闭合度的残差为13%.研究表明饱和水汽压差(VPD)是影响能量通量的主要因素,其次是空气温度(T_a)和土壤温度(T_s),均与能量通量呈显著正相关;而植被对能量通量的影响表现在LE与叶面积指数(LAI)呈正相关,而H相反.

关键词：科尔沁沙地涡动相关系统水热通量波文比能量平衡比影响因素

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西农特色培养模式下行业导师发挥作用的路径探讨及实践

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黑龙江教育（高教研究与评估） 2024年第9期 34-38页

作者：齐西婷李征李建明西北农林科技大学陕西咸阳712100

文章通过对西农特色培养模式(“示范站—专家大院—科技小院”)和行业导师制度的深入分析,总结了行业导师在培养模式中的重要作用,并提出了具体的实践路径。研究结果表明,经过近3年的探索和实践,西农特色培养模式下行业导师指导作用成... 详细信息

文章通过对西农特色培养模式(“示范站—专家大院—科技小院”)和行业导师制度的深入分析,总结了行业导师在培养模式中的重要作用,并提出了具体的实践路径。研究结果表明,经过近3年的探索和实践,西农特色培养模式下行业导师指导作用成效显著。行业导师不仅在学生专业培养和实践能力提升方面发挥着至关重要的作用,而且在职业规划、人际交往和创新意识等方面也起到了积极影响。

关键词：西农特色培养模式行业导师专业学位研究生

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齿轮泵内部间隙泄漏的研究综述

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液压气动与密封 2024年第5期44卷 49-57页

作者：陈云鹏张中波陈亭西中国民用航空飞行学院航空工程学院四川德阳618307

在高压状态下,齿轮泵内部间隙增大会加剧泵内泄漏,严重影响其容积效率。首先介绍轴向间隙和径向间隙是造成泵内泄漏的2条主要途径,分析2种间隙内液体泄漏量与间隙值的数学模型。接着介绍轴向间隙的主要影响因素包括:齿轮轴挠度变形、齿... 详细信息

在高压状态下,齿轮泵内部间隙增大会加剧泵内泄漏,严重影响其容积效率。首先介绍轴向间隙和径向间隙是造成泵内泄漏的2条主要途径,分析2种间隙内液体泄漏量与间隙值的数学模型。接着介绍轴向间隙的主要影响因素包括:齿轮轴挠度变形、齿轮轴偏心和泵体磨损与结构变形;而径向间隙主要被齿轮泵端盖变形和多物理场耦合作用所影响。为了控制泵内间隙泄漏量,总结了优化和控制内部间隙的研究内容。最后指出了齿轮泵间隙的局部大小变化,对研究泵的流量特性和内部结构设计有重要的参考意义。

关键词：齿轮泵轴向泄漏径向泄漏间隙优化间隙补偿

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SERPINH1 promoted the proliferation and metastasis of colorectal cancer by activating PI3K/Akt/mTOR signaling pathway

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World Journal of Gastrointestinal Oncology 2024年第5期16卷 1890-1907页

作者： xiao-Sheng Jin Lu-xi Chen ting-ting Ji Rong-Zhou Li Department of Gastroenterology The Third Affiliated Hospital of Wenzhou Medical UniversityRuian 325000Zhejiang ProvinceChina

BACKGROUND Serpin peptidase inhibitor clade H member 1(SERPINH1)was initially recognized as an oncogene implicated in various human malignancies.Nevertheless,the clinical relevance and functional implications of SERPINH1 in colorectal cancer(CRC)remain largely elusive.AIM To investigate the effects of SERPINH1 on CRC cells and its specific mechanism.METHODS Quantitative real-time polymerase chain reaction,western blotting analysis,The Cancer Genome Atlas data mining and immunohistochemistry were employed to examine SERPINH1 expression in CRC cell lines and tissues.A series of in-vitro assays were performed to demonstrate the function of SERPINH1 and its possible mechanisms in CRC.RESULTS SERPINH1 demonstrated elevated expression levels in both CRC cells and tissues,manifested at both mRNA and protein tiers.Elevated SERPINH1 levels correlated closely with advanced T stage,lymph node involvement,and distant metastasis,exhibiting a significant association with poorer overall survival among CRC patients.Subsequent investigations unveiled that SERPINH1 overexpression notably bolstered CRC cell proliferation,invasion,and migration in vitro,while conversely,SERPINH1 knockdown elicited the opposite effects.Gene set enrichment analysis underscored a correlation between SERPINH1 upregulation and genes associated with cell cycle regulation.Our findings underscored the capacity of heightened SERPINH1 levels to expedite G1/S phase cell cycle progression via phosphatidylinositol 3-kinase/AKT/mechanistic target of rapamycin pathway activation,thereby facilitating CRC cell invasion and migration.CONCLUSION These findings imply a crucial involvement of SERPINH1 in the advancement and escalation of CRC,potentially positioning it as a novel candidate for prognostic assessment and therapeutic intervention in CRC management.

关键词： Serpin peptidase inhibitor clade H member 1 Colorectal cancer Proliferation Cell cycle Phosphatidylinositol 3-kinase/AKT/mechanistic target of rapamycin

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Inhibitory effect on subretinal fibrosis by anti-placental growth factor treatment in a laser-induced choroidal neovascularization model in mice

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International Journal of Ophthalmology(English edition) 2022年第2期15卷 189-196页

作者： Yi Zhang Ding-Ying Liao Jian-Ming Wang Li-Jun Wang xi-ting Yang Ai-Yi Zhou Department of Ophthalmology the Second Affiliated Hospital of Xi’an Jiaotong University

AIM:To investigate whether anti-placental growth factor(PGF) can inhibit subretinal fibrosis and whether this effect is mediated by the inhibitory effect of PGF on epithelial-mesenchymal transition(EMT) of retinal pigment epithelial(RPE) cells.METHODS:Subretinal fibrosis model was established in laser induced choroidal neovascularization(CNV) mice on day 21 after laser photocoagulation.Immunofluorescence staining(IFS) of cryosections and enzyme-linked immunosorbent assay(ELISA) were used to detect the expression of PGF.IFS of whole choroidal flat-mounts was used to detect the degree of subretinal fibrosis.IFS of cryosections and ELISA were used to detect the expression of EMT related indicators in subretinal fibrosis lesions.RESULTS:The expression of PGF protein in subretinal fibrosis lesions was significantly up-regulated(P<0.05),and mainly co-stained with pan-cytokeratin labeled RPE cells.Intravitreal injection of anti-PGF neutralizing antibody reduced the area of subretinal fibrosis and the ratio of fibrotic/angiogenic area significantly at the concentrations of 0.25,0.5,1.0,and 2.0 μg/μL(all P<0.05).The expression of E-cadherin in the local RPE cells decreased,while α-SMA increased significantly in subretinal fibrosis lesions,and the application of anti-PGF neutralizing antibody could reverse these changes(P<0.05).CONCLUSION:The expression of PGF is up-regulated in the lesion site of subretinal fibrosis and mainly expressed in RPE cells.Intravitreal injection of anti-PGF neutralizing antibody can significantly inhibit the degree of subretinal fibrosis in CNV mice,and this effect may be mediated by the inhibition of PGF on EMT of RPE cells.

关键词： placental growth factor subretinal fibrosis epithelial mesenchymal transformation choroidal neovascularization

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Pancreatic cancer incidence and mortality patterns in 2006-2015 and prediction of the epidemiological trend to 2025 in China

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World Journal of Clinical Cases 2022年第14期10卷 4404-4413页

作者： Min-Yue Yin Li-ting xi Lu Liu Jin-Zhou Zhu Li-Juan Qian Chun-Fang Xu Department of Gastroenterology The First Affiliated Hospital of Soochow UniversitySuzhou 215006Jiangsu ProvinceChina

BACKGROUND Due to dietary patterns,the aging population,and other high-risk factors,the occurrence of pancreatic cancer(PC)has been rapidly increasing in China.AIM To present the epidemiological trends of PC in China over the past decade and the estimated trend in 2025 and to compare the international differences in PC morbidity and mortality.METHODS This study used a series of nationally representative data from the National Central Cancer Registry of China(NCCR),the International Agency for Research on Cancer and the Institute for Health Metrics and Evaluation databases.Agestandardized data of the PC incidence and mortality from 2006 to 2015 in China were extracted from the NCCR database.Linear regression models were used to estimate the incidence and mortality rates of PC in 2025.RESULTS The age-standardized rates of PC in China increased from 3.65 per 100000 in 2006 to 4.31 per 100000 in 2015 and were estimated to reach up to 5.52 per 100000 in 2025.The mortality went from 3.35 per 100000 in 2006 to 3.78 per 100000 in 2015,estimated to reach up to 4.6 per 100000 in 2025.The number of new cases and deaths was low before 45 years and the peak age of onset was 85-89 years.The incidence and mortality rates in men were higher than those in women regardless of the region in China.In addition,the incidence and mortality rates in China were higher than the average level around the world.Likewise,disabilityadjusted life years attributed to PC in China were 197.22 years per 100000,above the average level around the world.CONCLUSION This study presented an increasing trend of PC in China and differences in morbidity,mortality and disability-adjusted life years between Chinese and global populations.Efforts need to be made to decrease the PC incidence and improve patient outcomes.

关键词： Pancreatic cancer China Epidemiology Incidence Mortality

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