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Food Science and Human Wellness 2024年

作者： Alex Xiong Gao Tracy Chenxi Xia Maggie Suisui Guo Gary Kawing Yuen Kevin Qiyun Wu tina tingxia dong Karl Wah-Keung Tsim Shenzhen Key Laboratory of Edible and Medicinal Bioresources HKUST Shenzhen Research Institute Division of Life Science and Center for Chinese Medicine The Hong Kong University of Science and Technology

This study aims to uncover the hormetic process of luteolin， a common dietary flavone， in neuronal cells through its role in inducing mitochondrial stress. In rat pheochromocytoma PC12 cells， luteolin at low concentrations caused a mild and reversible loss of mitochondrial membrane potential (MMP)， while high concentrations of luteolin triggered intense and sustained depolarization of MMP. The MMP disturbance was shown to have a close association with the trophic and/or toxic effects triggered by luteolin; because the common mitochondrial uncouplers shared similar bi-phase dose-response on cell viability， as that of luteolin. Along with the induced MMP pertubation， luteolin triggered the development of autophagy and mitophagy， as determined by mCherry-GFP-LC3B tandem protein， and by the co-localization of mitochondrial/lysosomal staining. Subsequent application of autophagy inhibitors in the cultures blocked the luteolin-induced neurotrophic activities and sensitized the cells to be less resistant to luteolin-mediated cytotoxicity. Other flavonoids also displayed similar properties in the cultures， indicating that these compounds act as hormetic pharmacological inducers that stimulate cells to become more resilient and adapt to threats. This study provides a unifying mechanistic explanation for the neuro-beneficial effects of luteolin and other flavonoids.

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An optimization of ultra-sonication-assisted extraction from flowers of Apocynum venetum in targeting to amount of free amino acids determined by UPLC-MS/MS

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Food Quality and Safety 2019年第1期3卷 52-60页

作者： Yan Jin Caroline Yang Wang Weihui Hu Yun Huang Miranda Li Xu Huaiyou Wang Xiangpeng Kong Yicun Chen tina tingxia dong Qiwei Qin Karl Wah Keung Tsim Shenzhen Key Laboratory of Edible and Medicinal Bioresources HKUST Shenzhen Research InstituteHi-Tech ParkNanshanShenzhen518000China Division of Life Science and Center for Chinese Medicine The Hong Kong University of Science and TechnologyClear Water BayHong KongChina Pharmacology Department Shantou University Medical CollegeShantou515041China Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation College of Marine SciencesSouth China Agricultural UniversityGuangzhou 510642PR China

Amino acids are naturally occurring compounds in many edible or medicinal plants,which possess a variety of nutraceutical effects in ***,a method of ultrasound-assisted extraction was optimized using Box-Behnken design of response surface methodology in maximizing the yield of free amino acids deriving from flowers of Apocynum venetum *** the optimal condition of ultrasound-assisted extraction,i.e.187.5 W of ultrasonic power,31.93 min of extraction time,and 0.47 mg/ml of solid-liquid ratio,the experimental yield of extractive was 287.17±0.205 mg/g,which was in close agreement with the value,as predicted by the established *** addition,a hydrophilic interaction ultra-high performance liquid chromatography coupled with QqQ-MS/MS method was developed and validated for simultaneous quantification of 20 types of amino acids without derivatization contained in *** *** analytical method was validated by matrix effect,linearity,limit of detection,limit of quantification,precision,repeatability,stability,and *** analysis results showed that *** flower was rich in free amino acids of~3%of total dried weight,and which contained leucine(13.7 g/mg),isoleucine(7.9 g/mg),and lysine(2.2 g/mg)as the most abundant amino ***,*** flower could provide beneficial nutrient values for human health,*** weight maintenance or glucose homeostasis.

关键词： Apocynum venetum response surface methodology ultra-sonication-assisted extraction amino acid UPLC-MS/MS

Edible Bird’s Nest, an Asian Health Food Supplement, Possesses Skin Lightening Activities: Identification of N-Acetylneuraminic Acid as Active Ingredient

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Journal of Cosmetics, Dermatological Sciences and Applications 2015年第4期5卷 262-274页

作者： Gallant Kar Lun Chan Zack Chun Fai Wong Kelly Yin Ching Lam Lily Kwan Wai Cheng Laura Minglu Zhang Huangquan Lin tina tingxia dong Karl Wah Keung Tsim Division of Life Science and Center for Chinese Medicine R&D The Hong Kong University of Science and Technology Kowloon Hong Kong China

Edible bird’s nest (EBN;Yan Wo), or cubilose, is originated from the salivary secretion of Aerodramus fuciphagus. In Asia, EBN is famous for its unproven skin lightening function. Here, we aim to reveal the active ingredients of EBN responsible for skin lightening function. Three major fractions were isolated from EBN water extract by chromatography using LC-MS/MS, bioactivities of these isolated fractions were analyzed by assays of tyrosinase, melanocytes and 3D human skin model, from which, N-acetylneuraminic acid (NANA), the second isolated fraction showed an inhibition effect on tyrosinase activity in a dose-dependent manner. The IC50 of tyrosinase originated from mushroom and human was 16.93 mM and 0.10 mM respectively. Furthermore, only EBN with higher content of NANA (e.g. White and Red EBN), but not EBN with less NANA (e.g. Grass EBN), showed promising skin lightening function. This is the first report to reveal NANA being an active ingredient of EBN on skin lightening function.

关键词： Cubilose N-Acetylneuraminic Acid Chemical Analysis Skin Lightening Human Skin Model

Studies on Elmination of Residual SO2 During Boiling Sulfur-fumigated Dioscoreae Rhizoma by Vinegar or Sour Medicinal Herbs

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Studies on Elmination of Residual SO2 During Boiling Sulfur-...

开心散联合氟西汀改善慢性压力应激小鼠抑郁样行为的效应评价研究

第十四届全国中药和天然药物学术研讨会

作者： Yuzhong Zheng Yong Zhang Zhenxia Zhang Liling Xie Wen-liang Zhou Zhendan He tina tingxia dong Karl Wahkeung Tsim Zhiyong Le Hui Cao Hanshan Normal University Sun Yat-sen University Kangmei Pharmaceutical Research Institute Co. Ltd Shantou University Shenzhen University The Hong Kong University of Science and Technology National Engineering Research Center for Modernization of Traditional Chinese Medicine China Academy of Chinese Medical Sciences

Sulfur-fumigation that is used in TCM exhibits many advantages,including convenience,economy,high-efficiency,***,some sulfites can be remained in sulfur-fumigated TCM,which could damage human ***,there is no effective nethod to replace the sulfur-fumigation until ***,sulfur-fumigation is widely applied to TCMs,such as Dioscoreae Rhizoma,*** paper will reveal the dynamic;hanges of SO during boiling sulfur-fumigated Dioscoreae Rhizoma,and research the safety methods of eliminating residual sulfites in Dioscoreae *** showed that jnder the conventional cooking,residual sulfites in Dioscoreae Rhizoma could not be eliminated,and the eliminating rate of SO was only 1.95%,which mean the conventional soiling was difficult to eliminate residual sulfites in sulfur-fumigated Dioscoreae *** mL of hydrochloric acid(6 mol/L,pH = 0.48) was added in the decoction,then residual SO was completely eliminated in 30 min,which indicated pH was the key factor for emoving SO from *** results of acetic acid showed that 10 mL 20%acetic acid could completely remove SO.5 mL of vinegar in decoction(pH = 3.15) or 10 mL of vinegar n decoction(pH = 2.91) could eliminate SO to 75.97%and 87.66%,*** llustrated the effect of acetic acid and vinegar was very significant,but less than lydrochloric *** sour herbs(including Crataegi Fructus,Ziziphi Spinosae Semen,dry lemon) were tested,the eliminating rate of SO were 20.78%,14.29%and 61.30%,*** illustrated some sour herbs could be useful for removing SO from sulfur-fumigated Dioscoreae Rhizoma,and effect of dry lemon was ***,he paper provides effective methods:adding a small amount of vinegar or dry lemon can effectively eliminate SO in sulfur-fumigated Dioscoreae Rhizoma.

关键词： Dioscoreae Rhizoma Sulfur-fumigation Sulfur dioxide Eliminating rate vinegar sour medicinal herb

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世界科学技术-中医药现代化 2024年第1期26卷 31-39页

作者：李璇李鑫陈杨仝佳祥黄灵欣伍嘉慧董婷霞詹华强段金廒朱悦南京中医药大学药学院江苏省方剂研究重点实验室/江苏省方剂高技术研究重点实验室/中药资源产业化与方剂创新药物国家地方联合工程研究中心/江苏省中药资源产业化过程协同创新中心南京210023 香港科技大学生命科学部中药研发中心中国香港999077

目的考察中药复方开心散联合用药氟西汀改善慢性压力应激小鼠抑郁样行为的效用与作用机制。方法采用慢性压力应激构建抑郁小鼠模型,通过糖水偏嗜实验评价开心散与氟西汀联合用药改善模型小鼠抑郁样行为的效用。采用ELISA法测定受试动物... 详细信息

目的考察中药复方开心散联合用药氟西汀改善慢性压力应激小鼠抑郁样行为的效用与作用机制。方法采用慢性压力应激构建抑郁小鼠模型,通过糖水偏嗜实验评价开心散与氟西汀联合用药改善模型小鼠抑郁样行为的效用。采用ELISA法测定受试动物血清、海马组织和下丘脑组织中皮质醇(Cortisol)、促肾上腺皮质激素(ACTH)和促肾上腺皮质激素释放因子(CRF)的表达。采用HE染色、尼氏染色和TUNEL染色法,观察模型小鼠海马神经元损伤情况。采用Western blot法考察小鼠海马组织中Caspase-3和Caspase-9等凋亡信号通路关键蛋白的表达情况。结果开心散与氟西汀联用能显著上调模型小鼠糖水偏嗜率;同时,联合用药可抑制脑内和血清中压力应激因子的上调,减少小鼠海马区因抑郁导致的细胞凋亡,调控海马区的凋亡信号通路。结论开心散联合氟西汀可有效改善抑郁模型动物的抑郁样行为,减少氟西汀临床用量。两者联用后抑制压力应激轴激活可能是联合用药抗抑郁的作用环节。

关键词：开心散氟西汀抑郁症联合用药

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棘脑通的抗抑郁作用及机制研究

中国医院用药评价与分析 2024年第1期24卷 55-61页

作者：黄雯高雄夏辰希段然董婷霞詹华强广州医科大学附属肿瘤医院药学部广州510095 香港科技大学生命科学部暨中药研发中心中国香港特别行政区999007

目的:考察主成分为沙棘和花生壳提取物的棘脑通的抗抑郁药效及作用机制。方法:(1)动物水平上,采用慢性不可预知温和应激(CUMS)抑郁模型小鼠,随机分为空白组、模型组、棘脑通给药组(棘脑通低浓度组、棘脑通高浓度组)和氟西汀组。通过体... 详细信息

目的:考察主成分为沙棘和花生壳提取物的棘脑通的抗抑郁药效及作用机制。方法:(1)动物水平上,采用慢性不可预知温和应激(CUMS)抑郁模型小鼠,随机分为空白组、模型组、棘脑通给药组(棘脑通低浓度组、棘脑通高浓度组)和氟西汀组。通过体重测试、糖水偏嗜实验和悬尾不动时间测试等行为学研究,考察棘脑通的抗抑郁作用。(2)细胞水平上,将不同浓度的棘脑通应用于PC12细胞、SH-SY5Y细胞、BV2细胞和RAW 264.7细胞,研究棘脑通对神经营养因子功能的促进作用,保护神经细胞、诱导神经分化的作用,以及抗氧化、抗炎作用。结果:(1)与空白组相比,模型组小鼠糖水偏嗜率、体重显著降低,悬尾不动时间、强迫游泳不动时间显著延长(P<0.01);棘脑通给药组小鼠糖水偏嗜率较模型组显著升高(P<0.05),棘脑通给药组、氟西汀组小鼠体重较模型组显著升高(P<0.01),棘脑通低、高浓度组小鼠悬尾不动时间较模型组显著缩短(P<0.01、P<0.05),棘脑通给药组小鼠强迫游泳不动时间较模型组显著缩短(P<0.01),上述差异均有统计学意义。(2)棘脑通与神经生长因子(5、50 ng/mL)协同使用时,PC12细胞分化程度、pNF200-Luc表达荧光素酶活性显著提高。棘脑通与脑源性神经营养因子、神经营养因子-3和神经营养因子-4(5 ng/mL)协同使用时,荧光素酶的活性显著提高。100~1 000μg/mL的棘脑通可显著降低β-淀粉样蛋白的聚集。采用叔丁基过氧化氢诱导PC12细胞的氧化损伤后,应用棘脑通可提高PC12细胞活性,且浓度越高,效果越好。采用Erastin诱导SH-SY5Y细胞铁死亡后,应用棘脑通可提高SH-SY5Y细胞活性,且浓度越高,效果越好。将脂多糖用于BV2、RAW 264.7细胞后,应用棘脑通可降低白细胞介素1β的mRNA水平、核因子κB转录活性,且具有浓度依赖性。结论:棘脑通具有抗炎、抗氧化,保护神经细胞,诱导神经微丝表达,增强神经营养因子样的作用;该药通过以上作用发挥抗抑郁效果。

关键词：沙棘和花生壳提取物棘脑通抗抑郁

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A Rapid Determination of 30 Prohibited Pesticide Residues in Lycii Fructus by UPLC-MS/MS

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Phyton-International Journal of Experimental Botany 2023年第2期92卷 423-437页

作者： Kesheng Lin Zhengqi Wang Li Wang Jiawen Zhou Lijuan Han Jingjing Wen tingxia dong Ran Duan Ning Li KMHD Shenzhen518128China Shenzhen Key Laboratory of Edible and Medicinal Bioresources HKUST Shenzhen Research InstituteShenzhen518057China Kangmei Pharmaceutical Co. Ltd.Jieyang515300China

Prohibited pesticide residues have become one of the main factors affecting the quality and safety of Lycii Fructus,However,rarely studies focus on the rapid determination of these ***,a total of 30 kinds of prohibited pesticide residues were determined by ultra-performance liquid chromatography tandem triple quadrupole mass spectrometry(UPLC-MS/MS)in five different process *** methods,chromatographic separation and detection conditions in mass spectrometry were all optimized *** the five different pretreatment methods,the first and third solid phase extraction failed to provide high recoveries of sulfosulfuron compounds(both lower than 60%).Recovery of chlorphenamidine by the Quick Easy Cheap Effective Rugged and Safe multiresidue method(QuEChERS)was lower than 60%,which did not meet the requirements of trace *** concentrations of 30 prohibited pesticides residues treated by straightforward and solid phase extraction showed good linearity in their corresponding ranges,with correlation coefficients over *** average recoveries of straightforward ranged from 78.13%to 110.9%,while RSD ranged from 1.3%to 16.9%,albeit poor purification was *** recovery yield from solid phase extraction was between 67.75%and 103.08%with RSD value from 0.8%to 14.0%,which met the requirements of trace determination,this method has good precision and *** results could be employed to other Traditional Chinese Medicines(TCMs)in detecting prohibited pesticide residues.

关键词： Lycii fructus prohibited pesticide residues UPLC-MS/MS

基于自适应卡尔曼算法的单探测器解耦控制技术研究

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红外与激光工程 2022年第6期51卷 335-342页

作者：董全睿王伟国陈飞陈涛张振东刘廷霞中国科学院长春光学精密机械与物理研究所吉林长春130033

为了提高激光通信跟瞄系统的跟踪性能,增强系统的抗扰动能力,提出基于卡尔曼滤波的单探测器复合轴控制方法。首先,对单探测器复合轴系统原理进行分析,通过误差传递函数验证了解耦算法的可行性;其次,为了改善脱靶量迟滞的影响,同时降低... 详细信息

为了提高激光通信跟瞄系统的跟踪性能,增强系统的抗扰动能力,提出基于卡尔曼滤波的单探测器复合轴控制方法。首先,对单探测器复合轴系统原理进行分析,通过误差传递函数验证了解耦算法的可行性;其次,为了改善脱靶量迟滞的影响,同时降低探测器实时处理的要求,提出一种自适应卡尔曼滤波算法;最后,根据探测器坐标系与快速反射镜坐标系之间的旋转变换关系,计算出粗精系统的解耦矩阵,并搭建一套桌面实验系统进行原理验证。实验结果表明:单探测器复合轴在0.1 Hz低频扰动条件下,精跟踪系统的相对位移不会超出反射镜偏转角度的临界值,跟踪误差由2.54μrad下降到0.86μrad。解耦控制能够提高系统跟踪精度并增强抗扰动能力。对于以后工程中的实际应用具有一定的指导意义。

关键词：激光通信跟瞄系统单探测器卡尔曼滤波解耦矩阵

Synthesis and Anti-inflammatory Activity of a Novel Series of 9,10-Dihydro-4H,8H-chromeno[8,7-e][1,3]oxazin-4-one Derivatives

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Chemical Research in Chinese Universities 2010年第2期26卷 268-271页

作者： LIU Xiao-ping WANG Ying LAN Hui-yu SONG Ai-hua TSIM Karl Wah-keung dong tina Ting-xia HU Chun Department of Organic Chemistry School of Pharmaceutical Engineering Shenyang Pharmaceutical University Shenyang 110016 P. R. China Tianjin Key Laboratory of Chemistry and Analysis of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine Tianjin 300193 P. R. China Department of Biology The Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong P. R. China

Flavonoids exhibit a wide range of biological activities including anti-inflammatory activity, while some 1,3-benzoxazine derivatives show anti-inflammatory activity. In order to explore the novel anti-inflammatory agents, based on the principles of bioisosterism and hybridization, ten novel ehromeno[8,7-e][1,3]oxazin-4-ones were syn- thesized and characterized with IR, ^1H NMR, MS and elemental analyses. The anti-inflammatory activities of the target compounds were evaluated via the croton oil-induced ear oedema test in Swiss mice. According to screened resuits, some target compounds show potent anti-inflammatory activity.

关键词： Anti-inflammatory activity Heterocycle Flavone Chromeno[8,7-e]oxazin-4-one

TiO_(2)对Si_(3)N_(4)-SiC_(w)复合材料致密化和力学性能的影响

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硅酸盐学报 2021年第12期49卷 2790-2796页

作者：张晶孙峰张伟儒董廷霞中材高新氮化物陶瓷有限公司山东淄博255000 哈尔滨工业大学材料科学与工程学院哈尔滨150001 山东工业陶瓷研究设计院有限公司山东淄博255000

以Yb_(2)O_(3)-Al_(2)O_(3)作为烧结助剂,采用烧结-热等静压工艺制备了具有高致密度和优异力学性能的Si_(3)N_(4)-SiC_(w)复合材料,研究了TiO_(2)含量对Si_(3)N_(4)-SiC_(w)复合材料致密化和力学性能的影响。结果表明:TiO_(2)的加入促进... 详细信息

以Yb_(2)O_(3)-Al_(2)O_(3)作为烧结助剂,采用烧结-热等静压工艺制备了具有高致密度和优异力学性能的Si_(3)N_(4)-SiC_(w)复合材料,研究了TiO_(2)含量对Si_(3)N_(4)-SiC_(w)复合材料致密化和力学性能的影响。结果表明:TiO_(2)的加入促进了Si_(3)N_(4)-SiC_(w)复合材料的致密化。随着TiO_(2)含量的增加,Si_(3)N_(4)-8%SiC_(w)复合材料的抗弯强度、断裂韧性和Vickers硬度均呈现出先提高后降低的变化趋势。当TiO_(2)含量(质量分数)为3%时,Si_(3)N_(4)-8%SiC_(w)复合材料的相对密度和综合力学性能最佳,其相对密度、抗弯强度、断裂韧性和Vickers硬度分别为(99.5±0.1)%、(1 301±87) MPa、(7.8±0.1) MPa·m^(1/2)、(16.2±0.1) GPa。TiO_(2)和Si_(3)N_(4)之间的原位反应生成的SiO_(2)和TiN对Si_(3)N_(4)-SiC_(w)复合材料的界面结合强度及力学性能产生了重要影响。

关键词：氮化硅碳化硅晶须致密化力学性能烧结-热等静压