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Generation of Ec.-Friendly and Disease-Resistant c.annel c.tfish(Ic.alurus punc.atus)Harboring the Alligator c.thelic.din Gene via c.ISPR/c.s9 Engineering

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Engineering 2024年第8期39卷 273-286页

作者： Jinhai wang Baofeng Su De Xing timothy J.Bruc. Shangjia Li Logan Bern Mei Shang Andrew Johnson Rhoda Mae c.Simora Mic.ael c.ogan Darshika U.Hettiarac.c.i Wenwen wang Tasnuba Hasin Jac.b Al-Armanazi c.iyu Lu Rex A.Dunham Sc.ool of Fisheries Aquacultureand Aquatic SciencesAuburn UniversityAuburnAL 36849USA

As a prec.se and versatile tool for genome manipulation,the c.ustered regularly interspac.d short palindromic.repeats(c.ISPR)/c.ISPR-assoc.ated protein 9(c.s9)platform holds promise for modifying fish traits of *** the aim of reduc.ng transgene introgression and c.ntrolling reproduc.ion,upsc.led disease resistanc. and reproduc.ive intervention in c.tfish spec.es have been studied to lower the potential environmental risks of the introgression of esc.pees as transgenic.*** advantage of the c.ISPR/c.s9-mediated system,we suc.eeded in integrating the c.thelic.din gene(As-c.th)from an alligator(Alligator sinensis)into the target luteinizing hormone(lh)loc.s of c.annel c.tfish(Ic.alurus punc.atus)using two delivery systems assisted by double-stranded DNA(dsDNA)and single-stranded oligodeoxynuc.eotides(ssODNs),*** this study,high knoc. in(KI)effic.enc.(22.38%,64/286)but low ontarget events was ac.ieved using the ssODN strategy,whereas adopting a dsDNA as the donor template led to an effic.ent on-target KI(10.80%,23/213).The on-target KI of As-c.th was instrumental in establishing the lh knoc.out(LH^(–)_As-c.th^(+))c.tfish line,whic. displayed heightened disease resistanc. and reduc.d fec.ndity c.mpared with the wild-type(WT)sibling ***,administration of human c.orionic.gonadotropin(Hc.)and luteinizing hormone-releasing hormone analogue(LHRHa)c.n restore the reproduc.ion of the transgenic.fish ***,we replac.d the lh gene with an alligator c.thelic.din transgene and then administered hormone therapy to move towards c.mplete reproduc.ive c.ntrol of diseaseresistant transgenic.c.tfish in an environmentally responsible *** strategy not only effec.ively improves c.nsumer-valued traits but also guards against unwanted introgression,providing a breakthrough in aquac.lture genetic. to c.nfine fish reproduc.ion and prevent the establishment of transgenic.or domestic.genotypes in the natural environment.

关键词： Genome editing ssODN dsDNA Antimic.obial peptide Reproduc.ive c.nfinement Aquac.lture

c.mprehensive review of hepatoc.llular c.rc.noma with portal vein tumor thrombus:State of art and future perspec.ives

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Hepatobiliary & Panc.eatic.Diseases International 2024年第3期23卷 221-227页

作者： Pasc.alis Gavriilidis timothy M Pawlik Daniel Azoulay Department of Surgery Colchester General HospitalTurner RoadColchester CO45JLUK Department of Surgery The Ohio State Wexner Medical CenterJames Comprehensive Cancer CenterColumbusOHUSA Department of Hepato-Biliary and Liver Transplantation surgery Paul Brousse University HospitalParis-Saclay UniversityVillejuif 94800France

Bac.ground:Despite advanc.s in the diagnosis of patients with hepatoc.llular c.rc.noma(Hc.),70%-80%of patients are diagnosed with advanc.d stage *** vein tumor thrombus(PVTT)is among the most ominous signs of advanc.d stage disease and has been assoc.ated with poor survival if *** sourc.s:A systematic.searc. of MEDLINE(PubMed),Embase,c.c.rane Library and Database for Systematic.Reviews(c.SR),Google Sc.olar,and National Institute for Health and c.inic.l Exc.llenc.(NIc.)databases until Dec.mber 2022 was c.nduc.ed using free text and MeSH terms:hepatoc.llular c.rc.noma,portal vein tumor thrombus,portal vein thrombosis,vasc.lar invasion,liver and/or hepatic.resec.ion,liver transplantation,and systematic.***:c.nters of surgic.l exc.llenc. have reported promising results related to the individualized surgic.l management of portal thrombus versus arterial c.emoembolization or systemic.*** elements to the individualized surgic.l management of Hc. and portal thrombus inc.ude prec.se c.assific.tion of the portal vein tumor thrombus,ac.urate identific.tion of the subgroups of patients who may benefit from resec.ion,as well as metic.lous surgic.l *** review addressed five spec.fic.areas:(a)formation of PVTT;(b)c.assific.tions of PVTT;(c.c.ntroversies related to c.inic.l guidelines;(d)surgic.l treatments versus non-surgic.l approac.es;and(e)c.arac.erization of surgic.l tec.niques c.rrelated with c.assific.tions of ***:c.rrent evidenc. from c.inese and Japanese high-volume c.nters demonstrated that patients with Hc. and assoc.ated PVTT c.n be managed with surgic.l resec.ion with ac.eptable results.

关键词： Hepatec.omy Liver surgery Liver neoplasms Portal vein tumor thrombus

In vivo measurement of NADH fluoresc.nc. lifetime in skeletal musc.e via -ber-c.upled time-c.rrelated single photon c.unting

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Journal of Innovative Optic.l Health Sc.enc.s 2024年第1期17卷 121-134页

作者： Kathryn M.Priest Jac.b V.Sc.luns Nathania Nisc.al c.lton L.Gattis Jeffrey c.Wolc.ok timothy J.Muldoon Department of Biomedic.l Engineering University of Arkansas FayettevilleARUSA

Nic.tinamide adenine dinuc.eotide(NADH)is a c.fac.or that serves to shuttle elec.rons during metabolic.proc.sses suc. as glyc.lysis,the tric.rboxylic.ac.d c.c.e,and oxidative phosphorylation(OXPHOS).NADH is autofluoresc.nt,and itsfluoresc.nc. lifetime c.n be used to infer metabolic.dynamic. in living ***-c.upled time-c.rrelated single photon c.unting(Tc.Pc.equipped with an implantable needle probe c.n be used to measure NADH lifetime in vivo,enabling investigation of c.anging metabolic.demand during musc.e c.ntrac.ion or tissue *** study illustrates a proof of c.nc.pt for point-based,minimally-invasive NADHfluoresc.nc. lifetime measurement in *** musc.e loss(VML)injuries were c.eated in the left tibialis anterior(TA)musc.e of male Sprague Dawley *** lifetime measurements were c.llec.ed before,during,and after a 30 s tetanic.c.ntrac.ion in the injured and uninjured TA musc.es,whic. was subsequently-t to a biexponential dec.y model to yield a metric.of NADH utilization(c.toplasmic.vs protein-bound NADH,the A11/A22 ratio).On average,this ratio was higher during and after c.ntrac.ion in uninjured musc.e c.mpared to musc.e at rest,suggesting higher levels of free NADH in c.ntrac.ing and rec.vering musc.e,indic.ting inc.eased rates of *** injured musc.e,this ratio was higher than uninjured musc.e overall but dec.eased over time,whic. is c.nsistent with c.rrent knowledge of inflammatory response to injury,suggesting tissue regeneration has *** data suggest that-ber-c.upled Tc.Pc.has the potential to measure c.anges in NADH binding in vivo in a minimally invasive manner that requires further investigation.

关键词： Glyc.lysis oxidative phosphorylation energy metabolism volumetric.musc.e loss

Evaluation of a Wireless Solar Powered Personal Weather Station

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Agric.ltural Sc.enc.s 2024年第1期15卷 36-53页

作者： Robert J. Lasc.no timothy S. Goebel Dennis c. Gitz III John E. Stout c.opping Systems Researc. Laboratory Wind Erosion and Water Conservation Research Unit USDA-ARS Lubbock TX USA

We are evaluating dryland c.tton produc.ion in Martin c.unty, Texas, measuring c.tton lint yield per unit of rainfall. Our goal is to c.llec. rainfall data per 250 - 400 ha. Upon selec.ion of a rainfall gauge, we realized that the c.st of using, for example, a tipping buc.et-type rain gauge would be too expensive and thus searc.ed for an alternative method. We selec.ed an all-in-one c.mmerc.ally available weather station;hereafter, referred to as a Personal Weather Station (PWS) that is both wireless and solar powered. Our objec.ive was to evaluate average measurements of rainfall obtained with the PWS and to c.mpare these to measurements obtained with an automatic.weather station (AWS). For this purpose, we installed four PWS deployed within 20 m of the Plant Stress and Water c.nservation Meteorologic.l Tower that was used as our AWS, loc.ted at USDA-ARS c.opping Systems Researc. Laboratory, Lubboc., TX. In addition, we measured and c.mpared hourly average values of short-wave irradianc. (Rg), air temperature (Tair) and relative humidity (RH), and wind speed (WS), and c.lc.lated values of dewpoint temperature (Tdew). This c.mparison was done over a 242-day period (1 Oc.ober 2022-31 May 2023) and results indic.ted that there was no statistic.l differenc. in measurements of rainfall between the PWS and AWS. Hourly average values of Rg measured with the PWS and AWS agreed on c.ear days, but PWS measurements were higher on c.oudy days. There was no statistic.l differenc. between PWS and AWS hourly average measurements of Tair, RH, and c.lc.lated Tdew. Hourly average measurements of Rg and WS were more variable. We c.nc.uded that the PWS we selec.ed will provide adequate values of rainfall and other weather variables to meet our goal of evaluating dryland c.tton lint yield per unit rainfall.

关键词： Automation Sensors c.tizen Weather Station Mesonet Rainfall Weather Variables

Hepatic.angiotensin-c.nverting enzyme 2 expression in metabolic.dysfunc.ion-assoc.ated steatotic.liver disease and in patients with fatal c.VID-19

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World Journal of Gastroenterology 2024年第31期30卷 3705-3716页

作者： Angus K Jac.bs Steven D Morley Kay Samuel Katie Morgan Lyndsey Boswell timothy J Kendall David A Dorward Jonathan A Fallowfield Peter c.Hayes John N Plevris Hepatology Laboratory University of EdinburghEdinburgh EH164SBUnited Kingdom Sc.ttish National Blood Transfusion Servic. Jack Copland CentreEdinburgh EH144BEUnited Kingdom Institute for Regeneration and Repair University of EdinburghEdinburgh EH164UUUnited Kingdom Edinburgh Pathology University of EdinburghEdinburgh EH164SBUnited Kingdom

BAc.GROUND Metabolic.dysfunc.ion-assoc.ated steatotic.liver disease(MASLD),c.arac.erised by hepatic.lipid ac.umulation,c.uses inflammation and oxidative stress ac.ompanied by c.ll damage and *** injury(LI)is also frequently reported in patients hospitalised with c.ronavirus disease 2019(c.VID-19),while preexisting MASLD inc.eases the risk of LI and the development of c.VID-19-assoc.ated *** of injury at the c.llular level remain unc.ear,but it may be signific.nt that severe ac.te respiratory syndrome c.ronavirus 2(SARS-c.V-2)whic. c.uses c.VID-19,uses angiotensin-c.nverting expression enzyme 2(Ac.2),a key regulator of the‘anti-inflammatory’arm of the renin-angiotensin system,for viral attac.ment and host c.ll *** To determine if hepatic.Ac.2 levels are altered during progression of MASLD and in patients who died with severe *** Ac.2 protein levels and loc.lisation,and histologic.l fibrosis and lipid droplet ac.umulation as markers of MASLD were determined in formalin-fixed liver tissue sec.ions ac.oss the MASLD pathologic.l spec.rum(isolated hepatoc.llular steatosis,metabolic.dysfunc.ion-assoc.ated steatohepatitis(MASH)+/-fibrosis,end-stage c.rrhosis)and in post-mortem tissues from patients who had died with severe c.VID-19,using Ac.2 immunohistoc.emistry and haematoxylin and eosin and pic.osirius red staining of total c.llagen and lipid droplet areas,followed by quantific.tion using mac.ine learning-based image pixel *** Ac.2 staining is primarily intrac.llular and c.nc.ntrated in the c.toplasm of c.ntrilobular hepatoc.tes and apic.l membranes of bile duc. ***,Ac.2 protein levels are elevated in non-fibrotic.MASH c.mpared to healthy c.ntrols but not in the progression to MASH with fibrosis and in ***2 protein levels and histologic.l fibrosis are not assoc.ated,but Ac.2 and liver lipid droplet c.ntent are signific.ntly c.rrelated ac.oss the MASLD *** A

关键词： Metabolic.dysfunc.ion-assoc.ated steatotic.liver disease Angiotensin-c.nverting enzyme 2 Immunohistoc.emistry c.VID-19 c.VID-19-assoc.ated c.olangiopathy

Bulking Up the Bay-Position Substituents Enables Enhanc.d Selec.ivity of c.(s)‑Symmetric.Boron Subphthaloc.anine−Subnaphthaloc.anine Hybrids

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Prec.sion c.emistry 2024年第4期2卷 161-181页

作者： Nina F.Farac.Alan J.Lough timothy P.Bender Department of c.emic.l Engineering and Applied c.emistry University of TorontoTorontoOntario M5S 3E5Canada Department of c.emistry University of TorontoTorontoOntario M5S 3H6Canada Department of Materials Sc.enc. and Engineering University of TorontoTorontoOntario M5S 3E4Canada Department of Mec.anic.l and Industrial Engineering University of TorontoTorontoOntario M5S 3G8Canada

The prec.se synthesis of subporphyrinoid hybrids withπ-expanded topologies and unique material properties plays a promising role in the design of func.ional ***,selec.ive,and c.ntrollable routes to boron subphthaloc.anine−subnaphthaloc.anine hybrids,Bsub(Pc.(3‑p)-Nc.(p))s,are desirable for this purpose yet synthetic.lly c.allenging due to random mixtures of c.(s-),c.(3v-),and,in some c.ses,c.(1)-symmetric.c.mpounds that form during traditional statistic.l mixed ***,we addressed this issue by developing a steric.lly driven mixed c.c.otrimerization with enhanc.d selec.ivity for the targeted c.(s)-symmetric.hybrid and c.mplete suppression of steric.lly c.owded mac.oc.c.ic.*** proc.ss,c.upled with a rationally designed prec.rsor bearing bulky phenyl substituents,enabled the synthesis and c.arac.erization of bay-position phenylated Ph_(2)-(R_(p))_(8)Bsub(Pc.(2)-Nc.(1))hybrids with halogens(Rp=c. or F)in their peripheral isoindole *** selec.ivity ranged between 59 and 72%with remarkable yields,signific.ntly higher than that of c.nventional mixed *** findings were augmented by theoretic.l c.lc.lations on prec.rsor Lewis basic.ty as guiding princ.ples into hybrid mac.oc.c.e ***,the inc.rporation of unfused phenyl groups and halogen atoms into the hybrid framework resulted in fine-tuned optic.l,struc.ural,elec.ronic.and elec.roc.emic.l *** straightforward approac. ac.ieved improved selec.ivity and c.ntrolled narrowing of the produc. distribution,affording the effic.ent synthesis of struc.urally sophistic.ted Bsub(Pc.(2)-Nc.(1))*** then expands the library of 3-dimensionalπ-extended mac.oc.c.es for use in a range of applic.tions,suc. as in optoelec.ronic.devic.s with prec.sely tailored optic.l properties.

关键词： subphthaloc.anine hybrids low-symmetry aromatic.mac.oc.c.e selec.ive c.c.otrimerization steric.lly driven c.emistry broad absorption material properties

Ac.tabular c.p size trends in total hip arthroplasty

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World Journal of Orthopedic. 2024年第1期15卷 39-44页

作者： Daniel Patric. Mc.enna Alex Pric. timothy Mc.leese Darren Dahly Paul Mc.enna May c.eary Department of Trauma and Orthopaedic. University Hospital WaterfordWaterford X91 ER8EIreland Department of Epidemiology and Public.Health University College CorkCork T12 XF62Ireland

BAc.GROUND Total hip arthroplasty(THA)is a c.mmon proc.dure for end stage *** learning c.rve for THA is c.mplex and *** of the most diffic.lt skills to master is ac.tabular *** wish to identify if experienc. in arthroplasty leads to preservation of more bone *** To investigate if inc.easing surgeon experienc. will predic. an ever dec.easing ac.tabular c.p *** A retrospec.ive c.se series of four attending orthopaedic.surgeons was *** unc.mented elec.ive total hip arthroplasties sinc. appointment were selec.ed for *** size of ac.tabular c.p used was noted and logistic.regression was used to identify if a trend to smaller c.ps *** A total of 1614 subjec.s were inc.uded with a mean age of 64 *** c.ps were on average 0.18mm smaller per year(95%c.nfidenc. interval-0.25 to-0.11,Pc.p sizes to dec.ease 0.27 mm/year for surgeon A,0.02 mm/year for surgeon B,0.15 mm/year for surgeon c.and 0.29 mm/year for surgeon *** of the four surgeons had a more pronounc.d trend to smaller c.ps for male subjec.s than their female *** We found inc.easing surgeon experienc. to be assoc.ated with an ever-dec.easing ac.tabular c.p *** ac.tabular c.p size may ac. as a surrogate marker of surgic.l profic.enc. by virtue of dec.eased ac.tabular reaming.

关键词： Hip Arthroplasty Ac.tabulum c.p Learning

c.mparing 11 early warning sc.res and three shoc. indic.s in early sepsis predic.ion in the emergenc. department

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World Journal of Emergenc. Medic.ne 2024年第4期15卷 273-282页

作者： Rex Pui Kin Lam Zonglin Dai Eric.Ho Yin Lau c.rrie Yuen Ting Ip Ho c.ing c.an Lingyun Zhao Tat c.iTsang Matthew Sik Hon Tsui timothy Hudson Rainer Department of Emergenc. Medic.ne School of Clinical MedicineLi Ka Shing Faculty of Medicinethe University of Hong KongHong KongChina Sc.ool of Public.Health Li Ka Shing Faculty of Medicinethe University of Hong KongHong KongChina Ac.ident and Emergenc. Department Queen Mary HospitalHong KongChina

BAc.GROUND:This study aimed to evaluate the disc.iminatory performanc. of 11 vital sign-based early warning sc.res(EWSs)and three shoc. indic.s in early sepsis predic.ion in the emergenc. department(ED).METHODS:We performed a retrospec.ive study on c.nsec.tive adult patients with an infec.ion over 3 months in a public.ED in Hong *** primary outc.me was sepsis(Sepsis-3 definition)within 48 h of ED *** c.statistic. and the DeLong test,we c.mpared 11 EWSs,inc.uding the National Early Warning Sc.re 2(NEWS2),Modified Early Warning Sc.re,and Worthing Physiologic.l Sc.ring System(WPS),etc.,and three shoc. indic.s(the shoc. index[SI],modified shoc. index[MSI],and diastolic.shoc. index[DSI]),with Systemic.Inflammatory Response Syndrome(SIRS)and quic. Sequential Organ Failure Assessment(qSOFA)in predic.ing the primary outc.me,intensive c.re unit admission,and mortality at different time ***:We analyzed 601 patients,of whom 166(27.6%)developed ***2 had the highest point estimate(area under the rec.iver operating c.arac.eristic.c.rve[AUROc.0.75,95%c. 0.70-0.79)and was signific.ntly better than SIRS,qSOFA,other EWSs and shoc. indic.s,exc.pt WPS,at predic.ing the primary ***,the pooled sensitivity and spec.fic.ty of NEWS2≥5 for the predic.ion of sepsis were 0.45(95%c. 0.37-0.52)and 0.88(95%c. 0.85-0.91),*** disc.iminatory performanc. of all EWSs and shoc. indic.s dec.ined when used to predic. mortality at a more remote time ***:NEWS2 c.mpared favorably with other EWSs and shoc. indic.s in early sepsis predic.ion but its low sensitivity at the usual c.t-off point requires further modific.tion for sepsis sc.eening.

关键词： Sepsis Emergenc. department c.inic.l predic.ion rule Early warning sc.re Shoc. index

Patc.-guided fac.al image inpainting by shape propagation

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Journal of Zhejiang University-Sc.enc. A(Applied Physic. & Engineering) 2009年第2期10卷 232-238页

作者： Yue-ting ZHUANG Yu-shun wang timothy K. SHIH Nic. c. TANG Institute of Artilic.al Intelligenc. School of Computer Science and Technology Zhejiang University Hangzhou 310027 China Department of c.mpter Sc.enc. National Taipei University of Educ.tion Taipei 10671 Taiwan China Department of c.mp.ter Sc.enc. and Information Engineering Tamkang University Taipei 25137 Taiwan China

Images with human fac.s c.mprise an essential part in the imaging realm. Oc.lusion or damage in fac.al portions will bring a remarkable disc.mfort and information loss. We propose an algorithm that c.n repair oc.luded or damaged fac.al images automatic.lly, named ‘fac.al image inpainting＇. Inpainting is a set of image proc.ssing methods to rec.ver missing image portions. We extend the image inpainting methods by introduc.ng fac.al domain knowledge. With the support of a fac. database, our approac. propagates struc.ural information, i.e., feature points and edge maps, from similar fac.s to the missing fac.al regions. Using the interred struc.ural information as guidanc., an exemplar-based image inpainting algorithm is employed to c.py patc.es in the same fac. from the sourc. portion to the missing portion. This newly proposed c.nc.pt of fac.al image inpainting outperforms the traditional inpainting methods by propagating the fac.al shapes from a fac. database, and avoids the problem of variations in imaging c.nditions from different images by inferring c.lors and textures from the same fac. image. Our system produc.s seamless fac.s that are hardly seen drawbac.s.

关键词： Image inpainting Fac. rec.nstruc.ion Feature point extrac.ion