检索结果-南通市图书馆

Rolling Circle Amplification on Biotin-Streptavidin Complexes Immobilized to Activated Cyclic Polyolefin Surfaces

Materials Sciences and Applications 2013年第9期4卷 538-548页

作者： Herin Oh Alec Cerchiari Desirée Sorensen timothy mon Cassandra L.Smith Molecular Biology Research Laboratory Boston UniversityBostonUSA Department of Biomedical Engineering Boston Uni-versityBostonUSA Department of Biology Boston UniversityBostonUSA

Cyclic polyolefin (COP) is an inexpensive hydrophobic material with low auto-fluorescence, high light transmittance and thermal stability, broad chemical resistance and no non-specific protein binding. Here, the hydrophobic alkane COP was modified to have carbonyl functionalities through oxygen plasma and chemical etching treatments to increase usefulness for chemical and biochemical applications. Then, biotin-hydrazide was used to create biotinylated surfaces that bound streptavidin. A biotinylated target oligonucleotide was subsequently bound to the immobilized biotin-streptavidin and ligation mediated rolling circle amplification-based (L-RCA) SNP detection was demonstrated.

关键词： Cyclic Polyolefin Ligation Mediated Rolling Circle Amplification SNP Detection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The need for data integration to address the challenges of climate change on the Guyana coast

引用

Geography and Sustainability 2021年第4期2卷 288-297页

作者： Temitope D.timothy Oyedotun Helene Burningham Department of Geography Faculty of Earth and Environmental SciencesUniversity of GuyanaP.O.Box 101110Turkeyen CampusGeorgetownGuyana Coastal and Estuarine Research Unit(CERU) Department of GeographyUniversity College LondonGower StreetLondonWC1E 6BTUnited Kingdom

Guyana’s capacity to address the impacts of climate change on its coastal environment requires the ability to mon-itor,quantify and understand coastal change over short-,medium-and *** the drivers of change in coastal and marine environment can be achieved through the accurate measurement and critical anal-yses of morphologies,flows,processes and *** manuscript presents a strategy developed to create a central resource,database and web-based platform to integrate data and information on the drivers and the changes within Guyana coastal and marine *** strategy involves four complimentary work pack-ages including data collection,development of a platform for data integration,application of the data for coastal change analyses and consultation with *** last aims to assess the role of the integrated data sys-tems to support strategic governance and sustainable *** is hoped that the output of this strategy would support the country’s climate-focused agencies,organisations,decision-makers,and researchers in their tasks and endeavours.

关键词： Climate adaptation Climate mitigation Data needs Data integration Web-based platform

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：