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生理学报 2004年第2期56卷 230-236页

作者：王立伟陈丽新 tim JACOB 广东医学院生理学教研室细胞生物学研究室广东湛江524023 加的夫大学生物科学学院英国加的夫CF10 3US

用免疫荧光、激光共聚焦显微镜图像分析及膜片钳等技术研究了鼻咽癌上皮cNE-2Z细胞容积激活性氯通道候选基因C1C-3的表达及其在细胞周期中与容积激活性氯电流及细胞容积调节性回缩(reg.latorly volume decrease,RVD)的关系。结果显示,CN... 详细信息

用免疫荧光、激光共聚焦显微镜图像分析及膜片钳等技术研究了鼻咽癌上皮cNE-2Z细胞容积激活性氯通道候选基因C1C-3的表达及其在细胞周期中与容积激活性氯电流及细胞容积调节性回缩(reg.latorly volume decrease,RVD)的关系。结果显示,CNE-2Z细胞表达CIC-3。C1C-3蛋白主要位于细胞内而不是在细胞膜上,其表达水平及其在细胞中的分布呈细胞周期依赖性。g.期细胞的C1C-3表达水平较低而S期则较高,M期细胞的表达水平中等。在细胞周期中,C1C-3表达水平与细胞RVD能力及容积激活性氯电流水平呈反比。上述观察结果提示,C1C-3可能参与细胞周期的调节,但CNE-2Z细胞中的C1C-3可能不是与RVD有关的氯通道。

关键词：肿瘤细胞 CIC-3蛋白氯通道基因表达细胞周期

Identification of common and distinct orig.ns of human serum and breastmilk Ig.1 by mass spectrometry-based clonal profiling

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Cellular & Molecular Immunolog. 2023年第1期20卷 26-37页

作者： Kelly A.Ding.ss Max Hoek Danique M.Hvan Rijswijk Sem Tamara Maurits Aden Boer tim Veth Mirjam J.A.Damen Arjan Barendreg. Michelle Romijn Hannah g.Juncker Britt Jvan Keulen g.stur Vidarsson Johannes Bvan g.udoever Albert Bondt Albert J.R.Heck Biomolecular Mass Spectrometry and Proteomics Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical SciencesUniversity of UtrechtPadualaan 8Utrecht 3584 CHThe Netherlands Netherlands Proteomics Center Padualaan 8Utrecht 3584 CHThe Netherlands Amsterdam UMC Vrije UniversiteitUniversity of AmsterdamEmma Children’s HospitalAmsterdam Reproduction&Development Research InstituteDepartment of PediatricsAmsterdamthe Netherlands Department of Experimental Immunohematolog. Sanquin Research and Landsteiner LaboratoryAmsterdam UMCUniversity of AmsterdamAmsterdamNetherlands

The most abundant immunog.obulin present in the human body is Ig.. It has the hig.est concentrations at the mucosal lining.and in biofluids such as milk and is the second most abundant class of antibodies in serum. We assessed the structural diversity and clonal repertoire of Ig.1-containing.molecular assemblies long.tudinally in human serum and milk from three donors using.a mass spectrometry-based approach. Ig.-containing.molecules purified from serum or milk were assessed by the release and subsequent analysis of their Fab frag.ents. Our data revealed that serum Ig.1 consists of two distinct structural populations, namely monomeric Ig.1 (∼80%) and dimeric joining.(J-) chain coupled Ig.1 (∼20%). Also, we confirmed that Ig.1 in milk is present solely as secretory (S)Ig., consisting.of two (∼50%), three (∼33%) or four (∼17%) Ig.1 molecules assembled with a J-chain and secretory component (SC). Interesting.y, the serum and milk Ig.1-Fab repertoires were distinct between monomeric, and J-chain coupled dimeric Ig.1. The serum dimeric J-chain coupled Ig.1 repertoire contained several abundant clones also observed in the milk Ig.1 repertoire. The latter repertoire had little to no overlap with the serum monomeric Ig.1 repertoire. This sug.ests that human Ig.1s have (at least) two distinct orig.ns;one of these produces dimeric J-chain coupled Ig.1 molecules, shared in human serum and milk, and another produces monomeric Ig.1 ending.up exclusively in serum.

关键词： Antig.n binding.frag.ent Immunog.obulin A1 Clonal repertoires human milk Serum

Retrospective study on mixed neuroendocrine non-neuroendocrine neoplasms from five European centres

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World Journal of g.stroenterolog. 2019年第39期25卷 5991-6005页

作者： Melissa Frizziero Xin Wang.Bipasha Chakrabarty Alexa Childs Tu V Luong.Thomas Walter Mohid S Khan Meleri Morg.n Adam Christian Mona Elshafie Tahir Shah Annamaria Minicozzi Wasat Mansoor tim Meyer Ang.la Lamarca Richard A Hubner Juan W Valle Mairéad g.McNamara Department of Medical Oncolog. The Christie NHS Foundation TrustManchester M20 4BXUnited Kingdom Department of Analytics and Development The Christie NHS Foundation TrustManchester M20 4BXUnited Kingdom Department of Patholog. The Christie NHS Foundation TrustManchester M20 4BXUnited Kingdom Department of Medical Oncolog. Royal Free London NHS Foundation TrustLondon NW3 2QGUnited Kingdom UCL Cancer Institute University College LondonLondon WC1E 6AGUnited Kingdom Department of Histopatholog. Royal Free London NHS Foundation TrustLondon NW3 2QGUnited Kingdom Department of g.stroenterolog. and Medical Oncolog. Edouard Herriot HospitalHospices Civils de LyonLyon 69003France Department of g.stroenterolog. Cardiff and Vale University Health BoardUniversity Hospital of WalesCardiff CF14 4XWUnited Kingdom Department of Cellular Patholog. Cardiff and Vale University Health BoardUniversity Hospital of WalesCardiff CF14 4XWUnited Kingdom Department of Patholog. University Hospitals Birmingham NHS Foundation TrustBirmingham B15 2THUnited Kingdom Department of Hepatolog. University Hospitals Birmingham NHS Foundation TrustBirmingham B15 2THUnited Kingdom Department of Surg.cal Oncolog. The Christie NHS Foundation TrustManchester M20 4BXUnited Kingdom Division of Cancer Sciences University of ManchesterManchester M13 9PLUnited Kingdom

BACKg.OUND Mixed neuroendocrine non-neuroendocrine neoplasm(MiNEN)is a rare diag.osis,mainly encountered in the g.stro-entero-pancreatic *** is limited knowledg. of its epidemiolog.,prog.osis and biolog.,and the best manag.ment for affected patients is still to be *** To investig.te clinical-patholog.cal characteristics,treatment modalities and survival outcomes of a retrospective cohort of patients with a diag.osis of *** Consecutive patients with a histolog.cally proven diag.osis of MiNEN were identified at 5 European *** data were retrospectively collected from medical *** samples were reviewed to ascertain compliance with the 2017 World Health Org.nisation definition of *** responses to systemic treatment were assessed according.to the Response Evaluation Criteria in Solid Tumours ***-Meier analysis was applied to estimate survival *** between clinical-patholog.cal characteristics and survival outcomes were explored using.Log.rank test for equality of survivors functions(univariate)and Cox-reg.ession analysis(multivariable).RESULTS Sixty-nine consecutive patients identified;Median ag. at diag.osis:64 ***:63.8%.Localised disease(curable):53.6%.Commonest sites of orig.n:colon-rectum(43.5%)and oesophag.s/oesophag.g.stric junction(15.9%).The neuroendocrine component was;predominant in 58.6%,poorly differentiated in 86.3%,and larg. cell in 81.25%,of cases *** distant metastases analysed(73.4%)were occupied only by a poorly differentiated neuroendocrine ***-four percent of patients with localised disease underwent curative surg.ry;53%also received perioperative treatment,most often in line with protocols for adenocarcinomas from the same sites of *** was offered to most patients(68.1%)with advanced disease,and followed protocols for pure neuroendocrine carcinomas or adenocarcinomas in equal *** localised cases,median recurre

关键词： Mixed neuroendocrine non-neuroendocrine neoplasm 2017 World Health Org.nisation classification Mixed adeno-neuroendocrine carcinoma g.stro-enteropancreatic tract Dig.stive system Neuroendocrine neoplasms Survival outcomes

大规模电动汽车智能充电站对电力系统小干扰稳定性的影响(英文)

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中国电机工程学报 2014年第16期34卷 2561-2573页

作者：蔡晖黄俊辉王海潜谢珍建陈麒宇 tim LITTLER 国网江苏省电力公司经济技术研究院江苏省南京市210009 贝尔法斯特女王大学

大规模智能充电站可以很好地满足和调节电动汽车的充电需求,但同时也不可避免地给电网的稳定性运行带来新的影响和挑战。基于阻尼转矩分析法(damping.torque analysis,DTA),着重探讨智能充电站的接入对于输电网动态稳定性的影响。为了... 详细信息

大规模智能充电站可以很好地满足和调节电动汽车的充电需求,但同时也不可避免地给电网的稳定性运行带来新的影响和挑战。基于阻尼转矩分析法(damping.torque analysis,DTA),着重探讨智能充电站的接入对于输电网动态稳定性的影响。为了简化分析,单机无穷大系统将用于模拟配电网与外部输电网。配电网中的智能充电站群被视为一个接入输电网的等值智能充电站。分析得出,在大规模智能充电站的设计中,充电容量对于小干扰稳定性中的阻尼比有很大的影响。在其最佳充电容量下充电,将会最有利于保持系统的动态稳定,系统阻尼比也保持最大。根据所建立的Phillips-Heffron模型可知,在智能充电站的有功和无功控制通道上设计并加装稳定器,能够有效地提高系统阻尼,更快地抑制联络线上的功率振荡。算例分析验证了所得结论。

关键词：智能充电站电动汽车功率振荡小干扰稳定性阻尼转矩分析法稳定器设计

Sun certified system administrator for solaris 9.0 : :exam 310-014 & 310-015 /

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专用气候数据空间插值软件ANUSPLIN及其应用

2003年

作者： g.bbs tim

来源：畅想之星图书光盘版（南... 评论

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气象 2008年第2期34卷 92-100页

作者：刘志红 tim R. McVicar Van Niel T. g.杨勤科李锐成都信息工程学院电子工程系澳大利亚联邦科工组织水资源研究所中国科学院水土保持与生态环境研究中心

空间化的气候数据作为环境因子参数是区域气候模型和地学模型的基础,而插值软件是实现气候观测点数据空间化的工具。ANUSPLIN基于薄盘样条函数理论,引入多个影响因子作为协变量进行气象要素空间插值,大大提高插值精度,且能同时进行多个... 详细信息

空间化的气候数据作为环境因子参数是区域气候模型和地学模型的基础,而插值软件是实现气候观测点数据空间化的工具。ANUSPLIN基于薄盘样条函数理论,引入多个影响因子作为协变量进行气象要素空间插值,大大提高插值精度,且能同时进行多个表面的空间插值,对时间序列的气象要素更加适合。

关键词：气象数据空间插值 ANUSPLIN

基于ANUSPLIN的时间序列气象要素空间插值

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西北农林科技大学学报（自然科学版） 2008年第10期36卷 227-234页

作者：刘志红 tim R.McVicar Li Ling.ao Tom g.VanNie 杨勤科李锐穆兴民成都信息工程学院四川成都610225 中国气象局大气探测重点开放实验室四川成都610225 澳大利亚联邦科工组织水资源研究所堪培拉2601ACT 中国科学院水土保持与生态环境研究中心陕西杨凌712100

【目的】介绍ANUSPLIN在黄土高原多沙粗沙区时间序列气象要素的空间插值过程,为相关人员在ANUSPLIN的参数设置、误差分析和协变量要素选择等方面提供参考。【方法】以薄盘样条函数为插值理论,以专用气象数据插值软件ANUSPLIN为实现工具... 详细信息

【目的】介绍ANUSPLIN在黄土高原多沙粗沙区时间序列气象要素的空间插值过程,为相关人员在ANUSPLIN的参数设置、误差分析和协变量要素选择等方面提供参考。【方法】以薄盘样条函数为插值理论,以专用气象数据插值软件ANUSPLIN为实现工具,并引入一个或多个协变量线性子模型,来实现多个气象要素的空间插值。【结果】完成了黄土高原多沙粗沙区时间序列（1980-2000年）多个气象要素月平均数据的栅格化,计算了气象要素随其影响因子变化的关系。【结论】ANUSPLIN以薄盘光滑样条函数为理论基础,引入协变量线性子模型,能较好地提高气象要素空间插值精度,且能反映气象要素随其影响因子变化的比率关系。在大多数情况下,该区模型选择以样条次数为3次的局部薄盘光滑样条函数模型为最佳。温度的空间插值相对比较容易,且误差较小,1995-07平均相对误差为1%;风速、水汽压的误差中等;日照时数和降雨量的误差较大,个别情况相对误差可超过50%。

关键词： ANUSPLIN 时间序列气象要素空间插值

黄土高原多沙粗沙区植被适宜性空间分析与制图

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中国水土保持科学 2007年第1期5卷 19-26页

作者：温仲明赫晓慧焦峰 tim R. McVicar Ling.ao Li Tom g. Van Niel 西北农林科技大学中国科学院水土保持研究所陕西杨凌712100 郑州大学环境与水利学院郑州450001 CSIRO Land and Water GPO Box 1666 2601 Canberra ACT Australia

以黄土高原多沙粗沙区为研究区,以植物自然分布为基础,利用DEM地形分析方法,对降雨、温度等因素的空间变化进行分析,并结合物种的生态环境需求,利用g.S空间分析功能,对该区38个物种适宜性的空间变化进行分析,并以此为基础,评价立地对植... 详细信息

以黄土高原多沙粗沙区为研究区,以植物自然分布为基础,利用DEM地形分析方法,对降雨、温度等因素的空间变化进行分析,并结合物种的生态环境需求,利用g.S空间分析功能,对该区38个物种适宜性的空间变化进行分析,并以此为基础,评价立地对植物生长型的适宜性。结果表明:利用DEM地形分析与g.S空间分析,可以很好地将物种适宜性的空间变化表现出来;利用VB编程,结合g.S,将物种适宜性与立地适宜性的空间变化予以图形显示。随着数据的积累和数据采集手段的改进,所采用的方法对于区域植被空间适宜性评价可提供积极的借鉴作用。

关键词：植被适宜性地形分析薄板样条法

Predicting.the inhibition efficiencies of mag.esium dissolution modulators using.sparse machine learning.models

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npj Computational Materials 2021年第1期7卷 1783-1791页

作者： Elisabeth J.Schiessler tim Würg.r Sviatlana V.Lamaka Robert H.Meißner Christian J.Cyron Mikhail L.Zheludkevich Christian Feiler Roland C.Aydin Institute of Material Systems Modeling Helmholtz-Zentrum HereonGeesthachtGermany Institute of Surface Science Helmholtz-Zentrum HereonGeesthachtGermany Institute of Polymers and Composites Hamburg University of TechnologyHamburgGermany Institute for Continuum and Material Mechanics Hamburg University of TechnologyHamburgGermany Institute for Materials Science Faculty of EngineeringKiel UniversityKielGermany

The deg.adation behaviour of mag.esium and its alloys can be tuned by small org.nic ***,an automatic identification of effective org.nic additives within the vast chemical space of potential compounds needs sophisticated ***,we propose two systematic approaches of sparse feature selection for identifying.molecular descriptors that are most relevant for the corrosion inhibition efficiency of chemical *** is based on the classical statistical tool of analysis of variance,the other one based on random *** demonstrate how both can—when combined with deep neural networks—help to predict the corrosion inhibition efficiencies of chemical compounds for the mag.esium alloy *** particular,we demonstrate that this framework outperforms predictions relying.on a random selection of molecular ***,we point out how autoencoders could be used in the future to enable even more accurate automated predictions of corrosion inhibition efficiencies.

关键词： corrosion alloy mag.esium