检索结果-南通市图书馆

Neuroprotection by minocycline in murine traumatic spinal cord injury: analyses of matrix metalloproteinases

Neuroimmunology and Neuroinflammation 2017年第11期4卷 243-253页

作者： tiffany rice Jennifer EALarsen Hui Li Robert KNuttaII Peter HLarsen Steven Casha John HurIbert DyIan REdwards VWee Yong Department of Anesthesia Faculty of MedicineUniversity of CalgaryCalgaryAlberta T2N4N1Canada Department of Clinical Neurosciences Faculty of MedicineUniversity of CalgaryCalgaryAlberta T2N4N1Canada School of Biological Sciences University of East AngliaNorwich NR47TJUK Division of Neurosurgery University of ArizonaTucsonAZ85721USA.

Aim: Minocycline has neuroprotective activities in several models of neurological disorders including spinal cord injury (SCI) where it prevents axonal loss and improves functional recovery. There are still gaps of knowledge on minocycline in SCI including whether it ameliorates neuronal loss at the focal site of trauma, and whether minocycline reduces the activity of matrix metalloproteinases (MMPs), a family of enzymes implicated in the pathophysiology of SCI. This study addressed these gaps. Methods: Mice were treated with either minocycline or vehicle control after a spinal cord contusion. MMPs were compared between the two groups using real time polymerase chain reaction and zymography. Immunohistochemistry was used to examine microglial activation and neuronal cell death. Results: While several MMP members were elevated in the spinal cord following injury, treatment with minocycline did not affect their expression. Importantly, minocycline reduced the loss of neurons in the epicenter of damage to the spinal cord and in segments caudal and rostral to the injury. Conclusion: Despite the inability of minocycline to alter MMPs, the results of neuroprotection at the lesion site support the continued testing of minocycline as a neuroprotective medication in experimental and clinical SCI.

关键词： Minocycline matrix metalloproteinases spinal cord injury neuroprotection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Inverse analysis of laboratory data and observations for evaluation of backward erosion piping process

引用

Journal of Rock Mechanics and Geotechnical Engineering 2020年第5期12卷 1080-1092页

作者： Sige Peng John D.rice School of Civil Engineering and Transportation South China University of TechnologyGuangzhou510640China Department of Civil and Environmental Engineering Utah State UniversityLogan84322UTUSA

An inverse analysis procedure has been developed to interpret collected pore pressure data and observations during backward erosion piping(BEP)initiation and progression in sandy *** procedure has been applied to laboratory models designed to mimic the initiation and progression of BEP through a constricted vertical *** inverse analysis uses three-dimensional(3D)finite element method(FEM)to successively produce models of the hydraulic head regime surrounding progressive stages of BEP based on observations at the sample surface and pore pressure measurements obtained from the laboratory *** inverse analysis results in a series of 3D contour plots that represent the hydraulic-head regime at each stage of the BEP development,allowing for assessing the development of BEP mechanism as well as calculating the critical hydraulic conditions required for various BEP stages to initiate and *** of the results identified four significant stages of the piping process:(1)loosened zone initiation,(2)channel initiation and progression,(3)riser sand fluidization,and(4)loosened zone *** of the hydraulic head contour plots allows assessment of the critical hydraulic gradients needed to initiate and progress various components of the BEP development.

关键词： Backward erosion piping(BEP) Laboratory modeling Inverse analysis Finite element method(FEM) Soil loosening Critical gradient

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genetic analysis and gene mapping of a new rolled-leaf mutant in rice (Oryza sativa L.)

引用

Science China(Life Sciences) 2009年第9期52卷 885-890页

作者： SHI YongFeng1,2,CHEN Jie1,LIU WenQiang1,HUANG QiNa1,2,SHEN Bo2,LEUNG Hei3 & WU JianLi1 1 Chinese National Center for rice Improvement/National key Laboratory for rice Biology,China National rice Research Institute,Hangzhou 310006,China 2 College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 310016,China 3 International rice Research Institute,Metro Manila DAPO Box 7777,Philippines Chinese National Center for rice Improvement/National key Laboratory for rice Biology China National Rice Research Institute Hangzhou 310006 China College of Life and Environmental Sciences Hangzhou Normal University Hangzhou 310016 China International rice Research Institute Metro Manila DAPO Box 7777 Manila Philippines

To understand the development of rice leaf blades,we identified a new rolled-leaf mutant,w32,from indica cultivar IR64 through EMS mutagenesis. The mutant showed a stable rolled-leaf phenotype throughout the life cycle. Two F2 populations were developed by crossing w32 to cultivar IR24 and PA64. Genetic analysis showed that the rolled-leaf phenotype was controlled by a single recessive gene. To determine the location of the gene,bulked segregant analysis was carried out using mutant and wild-type DNA pools and 1846 mutant-type F2 individuals derived from the cross w32/PA64 were genotyped to locate the gene on the short arm of chromosome 7. The rolled-leaf gene,tentatively named rl11(t),is likely a new gene as no other rolled-leaf genes have been identified near the region. By developing new SSR and InDel markers,the gene was delimited to a 52 kb region near the end of the short chromosome arm. Further fine mapping and cloning of the gene are currently underway.

关键词： rice (Oryza sativa L.) chemical mutagenesis molecular marker rolled-leaf gene physical map

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecular identification of the cytoplasmic male sterile source from Dongxiang wild rice(Oryza rufipogon Griff.)

引用

Journal of Integrative Agriculture 2017年第8期16卷 1669-1675页

作者： YAN Song ZHU Shan MAO Ling-hua HUANG Ren-liang XIONG Hong-liang SHEN Lin-jun SHEN Xian-hua rice National Engineering Laboratory(Nanchang)/Nanchang Sub-center National Rice Improvement Center/Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics/Rice Research InstituteJiangxi Academy of Agricultural Sciences Key Laboratory of Crop Physiology Ecology and Genetic BreedingMinistry of Education/College of AgronomyJiangxi Agricultural University Jiangxi Super-rice Research and Development Center Jiangxi Academy of Agricultural Sciences

The use of cytoplasm male sterility （CMS） is crucial for three-line hybrid seed production. Two types of CMS have been discovered from Dongxiang wild rice, namely the wild abortion type CMS （CMS-WA） and the Dongxiang wild type CMS （CMS-DW）. In this study, we show the molecular identification of the two types of CMS in Dongxiang wild rice. WA352, which conferred CMS-WA, was not detected in Dongxiang wild iice, implying Dongxiang wild rice does not carry the CMS- WA source. Further analysis of WA352 in DYIA, a CMS-DW line, by PCR amplification and sequencing, revealed two insertion-deletion polymorphisms occurred in CMS-DW compared to WA352 of CMS-WA. It was reported that WA352 was comprised of an unknown origin sequence and partial sequences of three open reading frames （ORFs）, orf284, orf224 and orf288. The 42-bp insertion was located between the two segments of orf224 and orf288, which created a new chimeric ORF, orf216. This new ORF was also detected in CMS-HL. Based on the 9-bp deletion in orf284, a specific mitochon- drial marker of DW-M1 was developed, which could be used to specifically distinguish the DW-type source. Moreover, semi-quantitative RT-PCR analysis preliminarily suggested that both orf216 and orf284 could be considered as candidates for CMS-DW. These findings present a preliminary understanding of CMS-DW at the molecular level.

关键词： Dongxiang wild rice cytoplasm male sterile WA352 orf216 orf284 mitochondrial marker

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Genetics of Speciation： Considering Early-Acting Isolation, Hybrid Evolution, and Epigenetic Mechanisms

引用

Current Zoology 2013年第5期59卷 654-657页

作者： Amber M. rice, Editor Lehigh University Department of Biological Sciences Bethlehem PA 18015 USA amber.rice@Lehigh.edu

Since Darwin＇s time, evolutionary biologists have sought to understand how species arise. The study of speciation remains a focus of evolutionary biology, and since the early 204 century, characterizing the genetics underlying this process has also become a central goal. Much research into the genetics of speciation has cen- tered on identifying the genes underlying reproductive isolating mechanisms, or ＇speciation genes＇（see Nosil and Schluter, 2011 for a discussion of this term）. With information about such genes available from many sys- tems, general questions about how speciation proceeds may be addressed： How many genes contribute to speciation？ Is reproductive isolation more commonly the result of divergence in protein-coding regions or regulatory regions？ Is reproductive isolation more fre- quently the result of natural selection or genetic drift？（Coyne and Orr, 2004; Nosil and Schluter, 2011）. Given recent advances in technology, such questions have be- come accessible in systems that were previously intrac- table for studying the genetics of speciation. Moreover, with this increase in the accessibility of genetic analyses for non-model systems, a broader set of questions about the genetics of speciation can now be addressed.

关键词：遗传学特性生物学形态进化遗传机制基因漂移物种形成混合表观

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Conditional and unconditional mapping of quantitative trait loci underlying plant height and tiller number in rice (Oryza sativa L.) grown at two nitrogen levels

引用

Progress in Natural Science:Materials International 2008年第12期18卷 1539-1547页

作者： Hua Jiang~(a,b) Liang Jiang~b Longbiao Guo~b Zhenyu Gao~b Dali Zeng~b Lihuang Zhu~c Guohua Liang~a Qian Qian~b a State Key Laboratory of rice Biology/Key Laboratory of Plant Functional Genomies,Ministry of Education of China,Yangzhou University,Yangzhou 225009,China b State Key Laboratory of rice Biology,China National rice Research Institute,Hangzhou 310006,China c Institute of Genetics,Chinese Academy of Sciences,Beijing 100101,China State Key Laboratory of rice Biology/Key Laboratory of Plant Functional Genomics Ministry of Education of China Yangzhou University Yangzhou 225009 China State Key Laboratory of rice Biology China National Rice Research Institute Hangzhou 310006 China Institute of Genetics Chinese Academy of Sciences Beijing 100101 China

In this study,we used 127 double haploid (DH) lines to analyze agricultural traits of *** DH lines,derived from a ZYQ8 (indica)/JX17 (japonica) cross by anther culture,contained 160 RFLP and 83 SSR *** and conditional quantitative trait loci (QTL) mapping was conducted to analyze plant height (PH) and tillers per plant (TP) at five growth stages that were grown at two nitrogen *** PH and 13 TP unconditional QTL were identified in the different growth stages,including 19 QTL from high-nitrogen (HN) and 14 QTL from low-nitrogen (LN) *** conditional QTL for 14 genomic regions under LN/HN con- ditions showed that there was a significant effect on PH and TP across the different *** one conditional QTL,ph2-3,was unable to be detected in unconditional *** QTL were detected in the first four rice growth stages than in the final ***- more,a line from the DH mapping population,DH78,was identified in extreme phenotypes of PH and TP that exhibited dwarfism and less-tiller (dft) *** gene dft1 was mapped to chromosome 2 using a backcrossed population of DH78/JX17 through a map- based cloning *** location of dft1 coincided with the mapping region of the small-LOD peak,QTL ph2 and tp2,which were identified in plants grown in low-nitrogen *** backcrossing and fine-mapping successfully delimited the dft1 locus to a 91 kb region.

关键词： Oryza sativa Quantitative trait loci Double haploid (DH) population Developmental behavior Nitrogen Map-based cloning

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Studies on Rapid Quantitative Analysis of Bt Toxin by Using Envirologix Kits in Transgenic rice

引用

Agricultural Sciences in China 2002年第2期1卷 121-124页

作者： XIE Xiao-bo and SHU Qing-yao( Key Laboratory of Nuclear Agricultural Sciences , Ministry of Agriculture and Zhejiang Province ,Institute of Nuclear Agricultural Sciences , Zhejiang University, Hangzhou 310029 National rice Improvement Center ,China National rice Research Institute, Hangzhou 310006) Key Laboratory of Nuclear Agricultural Sciences Ministry of Agriculture and Zhejiang Province Institute of Nuclear Agricultural Sciences Zhejiang University Hangzhou 310029 National rice Improvement Center China National Rice Research Institute Hang Key Laboratory of Nuclear Agricultural Sciences Ministry of Agriculture and Zhejiang Province Institute of Nuclear Agricultural Sciences Zhejiang University Hangzhou 310029

Investigations were done on the usefulness of Envirologix CrylAb/CrylAc Plate kits for quantitative analysis of Bt toxin content in transgenic rice grains. Two transgenic rice lines: Kemingdao 1 (KMD1) and Kemingdao 2 (KMD2), transformed with a crylAb gene, and their parental variety, cv. Xiushui 11, were used as positive and negative samples. Results showed that the correlation coefficients as high as 0. 985 - 0. 998, significant at probability level of 0. 05 or 0.01, were obtained for linear regression equations by using the appended calibrators of the kits. No significant differences were detected for values of same rice sample obtained from different trials or by using different lots of the product (kit). The detectable Bt toxin content by this method could be as low as 0.5 ng/g. The Envirologix Kit could be useful for rapid quantitative detection of Bt toxin in rice grains because of its preciseness, simplicity and time saving.

关键词： Envirologix CrylAb/CrylAc plate kit Bt toxin Transgenic rice

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Enhancing disease resistances of Super Hybrid rice with four antifungal genes

引用

Science China(Life Sciences) 2007年第1期50卷 31-39页

作者： ZHU HuaChen1, XU XinPing1, XIAO GuoYing2,3, YUAN LongPing2 & LI BaoJian1 1 Biotechnology Research Center, Key Laboratory of Gene Engineering of Ministry of Education, Sun Yat-sen University, Guangzhou 510275, China 2 China National Hybrid rice R&D Center, Hunan Hybrid rice Research Center, Changsha 410125, China 3 Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha 410125, China 1. Biotechnology Research Center Key Laboratory of Gene Engineering of Ministry of Education Sun Yat-sen University Guangzhou 510275 China 2. China National Hybrid rice R&D Center Hunan Hybrid Rice Research Center Changsha 410125 China 3. Institute of Subtropical Agriculture the Chinese Academy of Sciences Changsha 410125 China

A plant expression vector harboring four antifungal genes was delivered into the embryogenic calli of ‘9311’, an indica restorer line of Super Hybrid rice, via modified biolistic particle bombardment. Southern blot analysis indicated that in the regenerated hygromycin-resistant plants, all the four anti-fungal genes, including RCH10, RAC22, β-Glu and B-RIP, were integrated into the genome of ‘9311’, co-transmitted altogether with the marker gene hpt in a Mendelian pattern. Some transgenic R1 and R2 progenies, with all transgenes displaying a normal expression level in the Northern blot analysis, showed high resistance to Magnaporthe grisea when tested in the typical blast nurseries located in Yanxi and Sanya respectively. Furthermore, transgenic F1 plants, resulting from a cross of R2 homo-zygous lines with high resistance to rice blast with the non-transgenic male sterile line Peiai 64S, showed not only high resistance to M. grisea but also enhanced resistance to rice false smut (a disease caused by Ustilaginoidea virens) and rice kernel smut (another disease caused by Tilletia barclayana).

关键词： Super Hybrid rice multi-gene transformation rice blast rice false smut rice kernel smut anti-fungi disease resistance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A Fusion Steganographic Algorithm Based on Faster R-CNN

引用

Computers, Materials & Continua 2018年第4期55卷 1-16页

作者： Ruohan Meng Steven G.rice Jin Wang Xingming Sun School of Computer and Software Nanjing University of Information Science and TechnologyNing Liu RoadNo.219Nanjing210044China Jiangsu Engineering Centre of Network Monitoring Ning Liu RoadNo.219Nanjing210044China Department of Mathematics and Computer Science Northeastern State University TahlequahOK 74464USA School of Computer&Communication Engineering Changsha University of Science&TechnologyChangsha410114China

The aim of information hiding is to embed the secret message in a normal cover media such as image,video,voice or text,and then the secret message is transmitted through the transmission of the cover *** secret message should not be damaged on the process of the cover *** order to ensure the invisibility of secret message,complex texture objects should be chosen for embedding *** this paper,an approach which corresponds multiple steganographic algorithms to complex texture objects was presented for hiding secret ***,complex texture regions are selected based on a kind of objects detection ***,three different steganographic methods were used to hide secret message into the selected block *** results show that the approach enhances the security and robustness.

关键词： Faster R-CNN fusion steganography object detection CNNs information hiding.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Xiangyou 63, a Quasi-aromatic Hybrid rice with Good Quality and High Yield

引用

杂交水稻 2004年第S1期 30-31页

作者： ZHOU Kun-lu, LIAO Fu-ming (Hunan Hybrid rice Research Center (HHRRC), Changsha, Hunan 410125, China)

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：