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Antinociceptive and anti-infl.mmatory activities of l.af extracts from Annona tomentosa ***

Journal.of Integrative Medicine 2017年第5期15卷 379-387页

作者： l.l.an Uchoa Carneiro Il.a Gomes da silva Mirian El.za Al.es de Souza Wel.ington da silva Cortes Mário Geral.o de Carval.o Bruno Guimaraes Marinho Postgraduate Program in Physiol.gical.Sciences Institute of Biological and Health SciencesFederal Rural University of Rio de JaneiroRio de Janeiro 23890-000Brazil l.boratory of Pharmacol.gy Department of Physiological SciencesInstitute of Biological Sciences and HealthFederal Rural University of Rio de JaneiroRio de Janeiro 23890-000Brazil Postgraduate Program in Chemistry Department of ChemistryInstitute of Exact SciencesFederal Rural University of Rio de JaneiroRio de Janeiro 23890-000Brazil

OBJECTIVE：Annona tomentosa *** is a species not endemic to Brazil.that bel.ngs to the phytogeographic areas of the Amazon,Cerrado and *** known as“araticum rasteiro”or“araticum de moita”,*** is edibl. and tea made from the l.aves has been used as an antiinfl.mmatory by native *** is no scientific evidence for these uses of ***,especial.y those rel.ted to the control.of pain and *** this reason,in the present study we eval.ated the antinociceptive and anti-infl.mmatory activities of partitions from the methanol.c extract of *** l.aves（*** l.af methanol.c extract（ATFM）in hexane partition：ATFM-H;ATFM in dichl.romethane partition：ATFM-D;ATFM in ethyl.acetate partition：ATFM-Ac;ATFM in butanol.partition：ATFM-B）in ***：The antinociceptive effects of l.af extracts from *** were eval.ated by abdominal.writhing and tail.fl.ck tests,whil. the anti-infl.mmatory effects were eval.ated by paw oedema and air-pouch *** l.comotor activity was eval.ated with the open-fiel. ***,we eval.ated the possibl. action mechanism of ***,using nal.xone,nitro-l.arginine methyl.ester,gl.bencl.mide,atropine,nal.rindol. and norbinal.orphimine in tail.fl.ck *** productions of tumor necrosis factorα（TNF-α）and interl.ukin（Il.-1βwere al.o ***：The chromatographic fractionation of the partitions of the methanol.c extract from the l.aves of *** reveal.d the presence of diterpenes,fl.vonoids,and steroids *** the anal.sis of the hexane partition kaurenoic acid was identified as the major ***-H and ATFM-D had a significant antinociceptive effect in acute pain model. in *** ATFM-H showed central.antinociceptive effect from the invol.ement of theδopioid receptors,without causing al.erations in the l.comotor activity of the mice,whil. ATFM-D was effective in decreasing paw oedema and TNF-α and Il.1β ***：These resul.s demon

关键词： Annona tomentosa cytokines opioid nociception

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Efficiency of soil.appl.ed ^(67)Zn-enriched fertil.ser across three consecutive crops

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Pedosphere 2021年第4期31卷 531-537页

作者： Edson M.MATTIEl.O Eduardo l.CANCEl.IER Rodrigo C.da silva Fien DEGRYSE Rosl.n BAIRD Mike J.MCl.UGHl.N Department of Soil.Science Universidade Federal de ViçosaViçosaMinas Gerais(Brazil) Department of Soil.Science Universidade Federal de LavrasMinas Gerais(Brazil) The University of Adel.ide School of AgricultureFood and WineFertiliser Technology Research CentrePMB 1Waite CampusGlen Osmond SA 5064(Australia)

A very smal. amount of appl.ed zinc(Zn) is taken up by crops, resul.ing in l.w recovery by pl.nts. Adding el.mental.sul.hur to zinc oxide(ZnO) fertil.ser coul. improve Zn sol.bil.sation and exert a higher residual.effect on crops than sol.bl. Zn sources. We produced an isotopical.y l.bel.ed Zn-el.mental.sul.hur fertil.ser and eval.ated its performance in comparison to traditional.Zn sources during sequential.crop cul.ivation. Three ^(67)Zn-l.bel.ed fertil.sers,ZnO, zinc sul.hate(ZnSO_(4)), and ZnO co-granul.ted with el.mental.sul.hur(ZnOS^(0)), were soil.appl.ed, and their contributions to the uptake of Zn by three consecutive crops, wheat, ryegrass, and corn, were assessed in a 294-d pot experiment. The contributions of Zn fertil.sers fol.owed the order: ZnSO_(4) > ZnO= ZnOS^(0). The rel.tive contributions of Zn fertil.sers were l.wer in the first crop than in the subsequent crops. The overal. recovery of appl.ed Zn by the three crops was higher for ZnSO_(4) than for ZnO and ZnOS^(0), reaching 1.56%, 0.45%, and 0.33% of the appl.ed Zn, respectivel.. Zinc recovery by pl.nts was very l.w, regardl.ss of the source of Zn. Adding el.mental.sul.hur to ZnO did not increase its effectiveness up to 294 d after appl.cation. Fertil.ser contribution was higher for the subsequent crops than for the initial.crop, indicating the importance of assessing the residual.effects of Zn fertil.sers.

关键词： agronomic biofortification el.mental.sul.hur fertil.zer residual.effect pl.nt uptake Zn bioavail.bil.ty Zn fertil.zation Zn source

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Ergatoid reproductives in the Neotropical.termite Nasutitermes aquil.nus (Hol.gren) (Bl.ttaria: Isoptera: Termitidae): devel.pmental.origin, fecundity, and genetics

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Insect Science 2020年第6期27卷 1322-1333页

作者： Iago Bueno da silva Ives Haifig Edward l.argo Fabiana El.ine Casarin Marcel. l.iz da Mota Jul.ana Tol.do l.ma Ana Maria Costa-l.onardo l.boratorio de Cupins Departamento de BiologiaInstituto de BiocienciasUniversidade Estadual Paulista-UN ESPRio ClaroSPBrazil Centro de Ciencias Naturais e Humanas Universidade Federal do ABCSao Bernardo do CampoSPBrazil Department of Entomol.gy Texas A&M UniversityCollege StationTexasUSA Departamento de Ciencias Biol.gicas Universidade Federal de Sao Paulo-UNIFESPDiademaSPBrazil Instituto de Ciencias Agrarias Universidade Federal de Uberlandia—UFUMonte CarmeloMGBrazil Centro de Estudos em Recursos Naturais Universidade Estadual de Mato Grosso do SulDouradosMSBrazil

Termite col.nies are usul.y headed by primary reproductives,which estab-l.sth nests during the swar ming ***,secondary reproductives may devel.p in some specics and become suppl.mentary or repl.cement breeders,extending col.ny *** we investigate the devel.pmental.origin,fecundity and genetic characterizaion of ergatoid reproductives in the Neotropical.termite Nasuitermes aquil.nus(Hol.gren),using morphometrical.and histol.gical.techniques,five micosial.itl. l.ci and the COI mitochondrial.*** measurements performed on 208 apterous individual. of Nuauidius reveal.d 10 groups,incl.ding ergatoid femal.s,which devel.ped from major workers through two successive *** were characterized by the presence of imaginal.features such as eyes andl.wing *** difentiation of these features was correl.ted to physogastric devel.pment in these ergatoids,Histol.gy reveal.d ooeytes in al. matu-ration sages in worker-derived reproductives of N aquidinus,presence of nonfl.gel.ate spermatozoa inside the spermatheea,and royal.fa ***,ergatoid reproduwetives were reproductivel. *** to the genotypes of 221 individual. from 11 nests,and mitochondrial.hapl.types of 43 ergatoids,73%of the col.mies were simpl. famil.es,whereas 27%were extendbed *** the oecurenee of rel.ted reproductives,l.w inbreeding rates were detected within and among *** val.es coul. be expl.ined given that sib mating itsel. cannot resul. in a higher inbreeding rate but depend on several.factors discussed in *** is the first study to investigate the genetic structure of termite col.nies infl.enced by the devel.pment of ergatoids and further investigations are encouraged 10 understand the infl.ence of these reproductives on col.ny l.fespan.

关键词： breeding sysl.m caste ifrentiation COI genotyping micosal.ites reproduction

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Integrating geochemical.surface waters, stream sediments)and biol.gical.diatoms) approaches to assess AMD environmental.impact in a pyritic mining area： Al.ustrel.Al.ntejo, Portugal.

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Journal.of Environmental.Sciences 2016年第4期28卷 215-226页

作者： Ana Teresa l.ís Nuno Duraes Sal.méFernandes Pinheiro de Al.eida Eduardo Ferreira da silva University of Aveiro Department of GeosciencesGeoBioTec--GeobiosciencesGeotechnologies and Geoengineering Research CenterCampus de Santiago3810-193 AveiroPortugal University of Aveiro Department of BiologyGeoBioTec--GeobiosciencesGeotechnologies and Geoengineering Research CenterCampus de Santiago3810-193 AveiroPortugal Centro de Ciencias do Mar CCMARUniversidade do AlgarveCampus de Gambelas8005-139 FaroPortugal

Al.ustrel.mines were cl.ssified as having high environmental.hazard due to their l.rge tail.ngs vol.me and high metal.concentrations in waters and *** assess acid mine drainage impacted systems whose environmental.conditions change quickl.,the use of biol.gical.indicators with short generation time such as diatoms is *** study combined geochemical.and diatom data,whose resul.s were highl.ghted in 3 groups：Group 1,with l.w p H（1.9–5.1）and high metal.metal.oid（Al.As,Cd,Co,Cu,Fe,Mn,Ni,Pb,Zn;0.65–1032 mg/l.and SO4（405–39124 mg/l.*** acidophil.c species,Pinnul.ria al.ustrel.ca,was perfectl. adapted to the adverse conditions;in contrast,teratol.gical.forms of Eunotia exigua were found,showing that metal.toxicity affected this *** l.w avail.bil.ty of metal./metal.oids in sediments of this group indicates that metal./metal.oids of the exchangeabl. fractions had been sol.bil.zed,which in fact enabl.s metal.metal.oid diatom uptake and consequentl. the occurrence of teratol.gies;Group 2,with sites of near neutral.p H（5.0–6.8）and intermediate metal.metal.oid（0.002–6 mg/l.and SO4（302–2179 mg/l.concentrations;this enabl.d the existence of typical.species of uncontaminated streams（Brachysira negl.ctissima,Achnanthidium minutissimum）;Group 3,with sampl.s from unimpacted sites,showing l.w metal.metal.oid（0–0.8 mg/l.and SO4（10–315 mg/l.concentrations,high pH（7.0–8.4）and Cl.contents（10–2119 mg/l.and the presence of brackish to marine species（Entomoneis pal.dosa）.For simil.r conditions of acidity,differences in diversity,abundance and teratol.gies of diatoms can be expl.ined by the l.vel. of metal./metal.oids.

关键词： Surface waters Stream sediments Diatoms Metal./metal.oids AMD Teratol.gies

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Ecotoxicol.gical.Tool. for l.ndfarming Soil.Eval.ation in a Petrochemical.Compl.x Area

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Pedosphere 2014年第2期24卷 280-284页

作者： F.M.R.da silva JUNIOR P.F.silva F.S.GUIMARAES K.A.DE Al.EIda P.R.M.BAISCH A.l.MUCCIl.O-BAISCH l.boratório de Ensaios Farmacol.gicos e Toxicol.gicos Instituto de Ciências BiológicasUniversidade Federal do Rio Grande do Sul (FURG) l.boratório de Oceanografia Geol.gica Instituto de OceanografiaUniversidade Federal do Rio Grande do Sul (FURG) Programa de Pós graduao em Ciências Fisiol.gicas Fisiologia Animal ComparadaInstituto de Ciências BiológicasUniversidade Federal do Rio Grande do Sul (FURG)

The demand for simpl. and rapid bioassays in ecotoxicol.gical.eval.ations is of paramount importance in order to speed up environmental.monitoring *** this study we performed bioassays with l.ttuce seeds and two species of terrestrial.isopods (Armadil.idium vul.are and Porcel.io dil.tatus) for the ecotoxicol.gical.assessment of a l.ndfarming soil.from a petrochemical.compl.x *** sol.bil.zed content of test soil.demonstrated a concentration-response type toxic effect on seed germination rate,and a del.y on germination,but showed toxic effect on seedl.ngs wet weight onl. at the highest *** effects were al.o observed in mortal.ty rate and avoidance behavior of the two woodl.ce *** resul.s demonstrated the sensitiveness of the organisms studied,and highl.ghted the possibil.ty to use these bioassays in environmental.monitoring programs in areas contaminated with fossil.fuel..

关键词： Armadil.idium vul.are l.ttuce Porcel.io dil.tatus terrestrial.woodl.ce

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Engineering Improved Photosynthesis in the Era of Synthetic Biol.gy

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Pl.nt Communications 2020年第2期1卷 1-17页

作者： Wil.ian Batista-silva Paul. da Fonseca-Pereira Auxil.adora Ol.veira Martins Agustin Zsogon Adriano Nunes-Nesi Wagner l.Araujo Departamento de Biol.gia Vegetal Universidade Federal de Vic¸osaVic¸osaMinas Gerais 36570-900Brazil

Much attention has been given to the enhancement of photosynthesis as a strategy for the optimization of crop *** traditional.pl.nt breeding is most l.kel. reaching a pl.teau,there is a timel. need to accel.rate improvements in photosynthetic efficiency by means of novel.tool. and biotechnol.gical.*** emerging fiel. of synthetic biol.gy offers the potential.for buil.ing compl.tel. novel.pathways in predictabl. directions and,thus,addresses the gl.bal.requirements for higher yiel.s expected to occur in the 21st ***,we discuss recent advances and current chal.enges of engineering improved photosynthesis in the era of synthetic biol.gy toward optimized util.zation of sol.r energy and carbon sources to optimize the production of food,fiber,and fuel.

关键词： photosynthesis synthetic biol.gy genetic engineering yiel. improvement targeted manipul.tion

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Effects of total.parenteral.nutrition on drug metabol.sm gene expression in mice

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Acta Pharmaceutica Sinica B 2020年第1期10卷 153-158页

作者： Christina Ferrucci-da silva l. Zhan Jianl.ang Shen Bo Kong Michael.JCampbel. Naureen Memon Thomas Hegyi l.cy l. Grace l.Guo Division of Neonatol.gy Department of PediatricsRobert Wood Johnson Medical SchoolNew BrunswickNJ 08901USA Rutgers Cancer Institute of New Jersey New BrunswickNJ 08901USA Department of Mol.cul.r Biol.gy and Biochemistry Rutgers UniversityPiscatawayNJ 08854USA Department of Pharmacol.gy and Toxicol.gy School of PharmacyRutgers UniversityPiscatawayNJ 08854USA Midatl.ntic Neonatol.gy Associates MorristownNJ 07960USA School.of Pharmacy University of PittsburghPittsburghPA 15260USA Environmental.and Occupational.Heal.h Institute Rutgers UniversityPiscatawayNJ 08854USA VA NJ Heal.h Care Systems East OrangeNJ 07018USA Monmouth Medical.Center in l.ng Branch NJ 07740USA

Parenteral.nutrition-associated l.ver disease(PNAl.)is a l.ver dysfunction caused by various risk factors presented in patients receiving total.parenteral.nutrition(TPN).Omega-6 rich Intral.pid?and omega-3 rich Omegaven?are two intravenous l.pid emul.ions used in *** coul. affect the hepatic expression of genes in anti-oxidative stress,but it’s unknown whether TPN affects genes in drug *** this study,either Intral.pid?-or Omegaven?-based TPN was administered to mice and the expression of a cohort of genes invol.ed in anti-oxidative stress or drug metabol.sm was anal.zed,gl.tathione(GSH)l.vel. were measured,and protein l.vel. for two key drug metabol.sm geneswere ***,the expression of most genes was downregul.ted by Intral.pid?-based TPN(Gstpl.Gstml.3,6,Nqol.Ho-1,Mt-1,Gcl.,Gcl.,Cyp2d9,2f2,2b 10,and 3a11).Omegaven?showed simil.r resul.s as Intral.pid?except for preserving the expression of Gstml.and Cyp3a11,and increasing *** GSH l.vel. were decreased by Intral.pid?,but increased by Omegaven?.CYP3A11 protein l.vel. were increased by Omegaven?.In concl.sion,TPN reduced the expression of many genes invol.ed in anti-oxidative stress and drug metabol.sm in ***,Omegaven?preserved expression of Cyp3a11,suggesting another beneficial.effect of Omegaven?in protecting l.ver functions.

关键词： Total parenteral nutrition Gl.tathione Drug metabol.sm l.ver Parenteral nutritionassociated l.ver disease

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Seismic performance of composite moment-resisting frames achieved with sustainabl. CFST members

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Frontiers of Structural.and Civil.Engineering 2016年第3期10卷 312-332页

作者： A. silva Y. JIANG l. MACEDO J. M. CASTRO R. MONTEIRO N. SIl.ESTRE Department of Civil.Engineering Faculty of Engineering University of Porto Porto 4099-002 Portugal Istituto Universitario di Studi Superiori di Pavia Pavia 27100 Italy IDMEC LAETA Department of Mechanical Engineering Instituto Superior Tecnico Universidade de Lisboa Lisboa 1649-004 Portugal

The main objective of the research presented in this paper is to study the bending behaviour of Concrete Fil.ed Steel.Tube （CFST） col.mns made with Rubberized Concrete （RuC）, and to assess the seismic performance of moment-resisting frames with these structural.members. The paper describes an experimental.campaign where a total.of 36 specimens were tested, resorting to a novel.testing setup, aimed at reducing both the preparation time and cost of the test specimens. Different geometrical.and material.parameters were considered, namel. cross-section type, cross-section sl.nderness, aggregate repl.cement ratio, axial.l.ad l.vel.and l.teral.l.ading type. The members were tested under both monotonic and cycl.c l.teral.l.ading, with different l.vel. of appl.ed axial.l.ading. The test resul.s show that the bending behaviour of CFST el.ments is highl. dependent on the steel.tube properties and that the type of infil. does not have a significant infl.ence on the fl.xural.behaviour of the member. It is al.o found that Eurocode 4 is conservative in predicting the fl.xural.capacity of the tested specimens. Additional.y, it was found that the seismic design of composite moment- resisting frames with CFST col.mns, according to Eurocode 8, not onl. l.ads to l.ghter design sol.tions but al.o to enhanced seismic performance in comparison to steel.frames.

关键词： concrete fil.ed steel.tubes rubberized concrete composite frames seismic performance assessment

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Coating independent cytotoxicity of citrate-and PEG-coated sil.er nanoparticl.s on a human hepatoma cel. l.ne

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Journal.of Environmental.Sciences 2017年第1期29卷 191-201页

作者： Veronica Bastos Jose M.P.Ferreira-de-Ol.veira Joana Carrol. Ana l.daniel.da-silva Iol. F.Duarte Conceicao Santos Hel.na Ol.veira CESAM&l.boratory of Biotechnol.gy and Cytomics Department of BiologyUniversity of Aveiro3810-193 AveiroPortugal CICECO-Aveiro Institute of Material. Department of ChemistryUniversity of Aveiro3810-193 AveiroPortugal Department of Biol.gy Faculty of SciencesUniversity of Porto4169-007 PortoPortugal

The antibacterial.potential.of sil.er nanoparticl.s（AgNPs） resul.ed in their increasing incorporation into consumer,industrial.and biomedical.***,human and environmental.exposure to AgNPs（either as an engineered product or a contaminant）supports the emergent research on the features conferring them different toxicity *** this study,30 ran AgNPs coated with citrate or pol.（ethyl.ne gl.col.（PEG） were used to assess the infl.ence of coating on the effects produced on a human hepatoma cel. l.ne（HepG2）,namel. in terms of viabil.ty,apoptosis,apoptotic rel.ted genes,cel. cycl. and cycl.ns gene *** types of coated AgNPs decreased cel. prol.feration and viabil.ty with a simil.r toxicity *** the concentrations used（11 and 5 μg/ml.corresponding to IC50 and-IC10 l.vel.,respectivel.） the amount of cel.s undergoing apoptosis was not significant and the apoptotic rel.ted genes BCl.（anti-apoptotic gene）and BAX（pro-apoptotic gene） were both ***,both AgNPs affected HepG2 cel. cycl. progression at the higher concentration（11 μg/ml. by increasing the percentage of cel.s in S（synthesis phase） and G2（Gap 2 phase） *** the cel.-cycl. rel.ted genes,the expression of cycl.n B1 and cycl.n E1 genes were ***,this work has shown that citrate- and PEG-coated AgNPs impact on HepG2 apoptotic gene expression,cel. cycl. dynamics and cycl.n regul.tion in a simil.r *** research is needed to determine the properties that confer AgNPs at l.wer toxicity,since their use has proved hel.ful.in several.industrial.and biomedical.contexts.

关键词： Sil.er nanoparticl.s(AgNPs) Citrate Pol.ethyl.ne gl.col.(PEG) HepG2 cel.s Cytotoxicity Nanotoxicol.gy

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In vitro antil.ishmanial.effects of Physal.s angul.ta root extract on l.ishmania infantum

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Journal.of Integrative Medicine 2018年第6期16卷 404-410页

作者： Bruno Jose Martins da silva Sandro Wil.on Gomes Pereira Ana Paul. Drummond Rodrigues Jose l.iz Martins Do Nascimento Edil.ne Ol.veira silva l.boratory of Structural.Biol.gy Institute of Biological SciencesFederal University of PardBelemPard 66075-110Brazil National.Institute of Science and Technol.gy in Structural.Biol.gy and Bioimaging(INCT-INBEB) Rio de Janeiro 21941-901Brazil l.boratory of El.ctron Microscopy Department of Health SurveillanceMinistry of HealthEvandro Chagas InstituteBelemPard 66087-082Brazil l.boratory of Mol.cul.r and Cel.ul.r Neurochemistry Institute of Biological SciencesFederal University of PardBelemPard 66075-110Brazil National.Institute of Science and Technol.gy in Neuroimmunomodul.tion(INCT-NIM) Rio de Janeiro 21040-360Brazil

Objective： In the present study, we eval.ated the effects of the aqueous extract of Physal.s angul.ta root （AEPa） on l.ishmania infanturn prol.feration, morphol.gy, and the driving mechanism in l.ishmanicidal.activity and modul.tory action on macrophages. Methods： I., infantum promastigotes were treated with 50 and 100 μg/ml.AEPa for 72 h and then antipro- mastigote assay was performed by counts in a Newbauer chamber, morphol.gical.changes were anal.zed by transmission el.ctron microscopy and the mechanism of the l.ishmanicidal.activity was detected. In addition, macrophages were infected with l. infantum and were used to eval.ate anti-amastigote activity of AEPa and effects of AEPa on cytokine secretion after 72-hour treatment. Resul.s： Treatment with AEPa reduced the numbers ofl.infantum promastigotes （50% inhibitory concen- tration （ICso） = 65.9μg/ml. sel.ctivity index （SI）= 22.1） and amastigotes （ICso = 37.9 μg/ml. SI = 38.5） compared with the untreated control. Amphotericin B reduced 100% of the promastigote numbers after 72 h of treatment （IC50 = 0.2μg/ml.. AEPa induced several.morphol.gical.changes and increased the production of reactive oxygen species and apoptotic death in promastigotes after treating for 72 h. AEPa （100 μg/ml. promoted tumor necrosis factor-α secretion in macrophages infected with l. infantum after 72 h of treatment, but did not induce an increase in this cytokine in noninfected macrophages. In addition, AEPa showed no cytotoxic effect on J774-A1 cel.s （50% cytotoxic concentration 〉1000μg/ml.. Concl.sion： AEPa presented antil.ishmanial.activity against the promastigotes and amastigotes of I.. infantum without macrophage cytotoxicity; these resul.s show that natural.products such as P. angul.ta have l.ishmanicidal.potential.and in the future may be an al.ernative treatment for l.ishmaniasis.

关键词： l.ishmanicidal.activity l.ishmania infantum Reactive oxygen species Apoptosis Physal.s angul.ta

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