检索结果-南通市图书馆

Comparative proteomic analysis of plasma exosomes reveals the functional contribution of N-acetyl-alpha-glucosaminidase to Parkinson’s disease

引用

Neural Regeneration Research 2025年第10期20卷 2998-3012页

作者： Yuan Zhao Yidan zhang Xin Liu Jian zhang Ya Gao Shuyue Li Cui Chang Xiang Liu Guofeng Yang Department of Geriatrics Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina Department of Neurosurgery The Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina

Parkinson’s disease is the second most common progressive neurodegenerative disorder,and few reliable biomarkers are available to track disease *** proteins,DNA,mRNA,and lipids carried by exosomes reflect intracellular changes,and thus can serve as biomarkers for a variety of *** this study,we investigated alterations in the protein content of plasma exosomes derived from patients with Parkinson’s disease and the potential therapeutic roles of these proteins in Parkinson’s *** a tandem mass tag-based quantitative proteomics approach,we characterized the proteomes of plasma exosomes derived from individual patients,identified exosomal protein signatures specific to patients with Parkinson’s disease,and identified N-acetyl-alpha-glucosaminidase as a differentially expressed protein.N-acetyl-alpha-glucosaminidase expression levels in exosomes from the plasma of patients and healthy controls were validated by enzyme-linked immunosorbent assay and western *** results demonstrated that the exosomal N-acetyl-alpha-glucosaminidase concentration was not only lower in Parkinson’s disease,but also decreased with increasing Hoehn-Yahr stage,suggesting that N-acetyl-alpha-glucosaminidase could be used to rapidly evaluate Parkinson’s disease ***,western blot and immunohistochemistry analysis showed that N-acetyl-alpha-glucosaminidase levels were markedly reduced both in cells treated with 1-methyl-4-phenylpyridinium and cells overexpressingα-synuclein compared with control ***,N-acetyl-alpha-glucosaminidase overexpression significantly increased cell viability and inhibitedα-synuclein expression in 1-methyl-4-phenylpyridinium-treated *** together,our findings demonstrate for the first time that exosomal N-acetyl-alpha-glucosaminidase may serve as a biomarker for Parkinson’s disease diagnosis,and that N-acetyl-alpha-glucosaminidase may reduceα-synuclein expression and 1-methyl-4-phenylpyridinium-induced n

关键词： biomarker diagnosis exosomes N-acetyl-alpha-glucosaminidase Parkinson’s disease proteomic α-synuclein

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Inhibiting ceramide synthase 5 expression in microglia decreases neuroinflammation after spinal cord injury

引用

Neural Regeneration Research 2025年第10期20卷 2955-2968页

作者： Wei zhang Yubao Lu Ruoqi Shen Yingjie Wu Chenrui Liu Xingxing Fang Liangming zhang Bin Liu Limin Rong Department of Spine Surgery The Third Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina National Medical Products Administration(NMPA)Key Laboratory for Quality Research and Evaluation of Cell Products GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Quality Control of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Engineering and Technology Research of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina

Microglia,the resident monocyte of the central nervous system,play a crucial role in the response to spinal cord ***,the precise mechanism remains *** investigate the molecular mechanisms by which microglia regulate the neuroinflammatory response to spinal cord injury,we performed single-cell RNA sequencing dataset analysis,focusing on changes in microglial *** found that the MG1 subpopulation emerged in the acute/subacute phase of spinal cord injury and expressed genes related to cell pyroptosis,sphingomyelin metabolism,and neuroinflammation at high ***,we established a mouse model of contusive injury and performed intrathecal injection of siRNA and molecular inhibitors to validate the role of ceramide synthase 5 in the neuroinflammatory responses and pyroptosis after spinal cord ***,we established a PC12-BV2 cell co-culture system and found that ceramide synthase 5 and pyroptosis-associated proteins were highly expressed to induce the apoptosis of neuron *** ceramide synthase 5 expression in a mouse model of spinal cord injury effectively reduced ***,ceramide synthase 5-induced pyroptosis was dependent on activation of the NLRP3 signaling *** ceramide synthase 5 expression in microglia in vivo reduced neuronal apoptosis and promoted recovery of neurological ***2g7 formed a“bridge”between sphingolipid metabolism and ceramide synthase 5-mediated cell death by inhibiting the NLRP3 signaling ***,these findings suggest that inhibiting ceramide synthase 5 expression in microglia after spinal cord injury effectively suppressed microglial pyroptosis mediated by NLRP3,thereby exerting neuroprotective effects.

关键词： ceramide synthase 5 gasdermin D microglia neuroinflammation NLRP3 nuclear factor kappa B Pla2g7 pyroptosis sphingomyelin metabolism spinal cord injury

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

泸州枳壳挥发油的取提工艺优化

引用

中国民族民间医药 2018年第23期27卷 52-55页

作者：张添植辜晓英李薪宇牛曼思税丕先泸州宇龙枳壳枳实研究院四川泸州646000 西南医科大学药学院四川泸州646000

目的:优选水蒸气蒸馏法提取枳壳挥发油的工艺,分析影响提取挥发油的因素。方法:以挥发油提取率为评价指标,粉碎度、加水量、提取时间为考察因素,采用正交试验考察水蒸气蒸馏法提取枳壳挥发油的工艺。结果:以粉碎度10目、加水量8倍、提... 详细信息

目的:优选水蒸气蒸馏法提取枳壳挥发油的工艺,分析影响提取挥发油的因素。方法:以挥发油提取率为评价指标,粉碎度、加水量、提取时间为考察因素,采用正交试验考察水蒸气蒸馏法提取枳壳挥发油的工艺。结果:以粉碎度10目、加水量8倍、提取时间7h,为枳实挥发油的最佳提取工艺。结论:优化的提取工艺重复性好,适用于枳壳挥发油的提取,可为开发枳壳挥发油新制剂提供依据。

关键词：枳壳挥发油正交试验提取工艺

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Direct Photolithography of WO_(x)Nanoparticles for High‑Resolution Non‑Emissive Displays

引用

Nano-Micro Letters 2025年第3期17卷 297-309页

作者： Chang Gu Guojian Yang Wenxuan Wang Aiyan Shi Wenjuan Fang Lei Qian Xiaofei Hu Ting zhang Chaoyu Xiang Yu‑Mo zhang Laboratory of Optoelectronic Information Technology and Devices Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingbo 315201People’s Republic of China Hangzhou Bay Laboratory of Advanced Nano‑Optoelectronic Materials and Devices Qianwan Institute of CNITECHNingbo 315336People’s Republic of China Smart Materials for Architecture Research Lab Innovation Center of Yangtze River DeltaZhejiang UniversityJiaxing 314100People’s Republic of China University of Science and Technology of China Hefei 230026People’s Republic of China State Key Lab of Supramolecular Structure and Materials College of ChemistryJilin UniversityChangchun 130012People’s Republic of China

High-resolution non-emissive displays based on electrochromic tungsten oxides(WOx)are crucial for future near-eye virtual/augmented reality interactions,given their impressive attributes such as high environmental stability,ideal outdoor readability,and low energy ***,the limited intrinsic structure of inorganic materials has presented a significant challenge in achieving precise patterning/pixelation at the micron ***,we successfully developed the direct photolithography for WOx nanoparticles based on in situ photo-induced ligand *** strategy enabled us to achieve ultra-high resolution efficiently(line width3600 cycles),as well as promising applications in electronic logos,pixelated displays,flexible electronics,*** success and advancements presented here are expected to inspire and accelerate research and development(R&D)in high-resolution non-emissive displays and other ultra-fine micro-electronics.

关键词： Electrochromic Direct photolithography WOx nanoparticles In situ photo-induced ligand exchange High-resolution displays

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A novel flexible nerve guidance conduit promotes nerve regeneration while providing excellent mechanical properties

引用

Neural Regeneration Research 2025年第7期20卷 2084-2094页

作者： Tong Li Quhan Cheng Jingai zhang Boxin Liu Yu Shi Haoxue Wang Lijie Huang Su zhang Ruixin zhang Song Wang Guangxu Lu Peifu Tang Zhongyang Liu Kai Wang Department of Orthopedics the Fourth Medical Center of Chinese PLA General HospitalBeijingChina Department of Training and Sports Medicine Characteristic Medical Center of Chinese People’s Armed Police ForceTianjinChina Medical School of Chinese PLA BeijingChina Key Laboratory of Bioactive Materials for the Ministry of Education College of Life SciencesNankai UniversityTianjinChina Department of Orthopedics Tianjin HospitalTianjinChina Department of Rehabilitation Medicine Er Quan Hospital of PAPWuxiJiangsu ProvinceChina

Autografting is the gold standard for surgical repair of nerve defects>5 mm in length;however,autografting is associated with potential complications at the nerve donor *** an alternative,nerve guidance conduits may be *** ideal conduit should be flexible,resistant to kinks and lumen collapse,and provide physical cues to guide nerve *** designed a novel flexible conduit using electrospinning technology to create fibers on the innermost surface of the nerve guidance conduit and employed melt spinning to align ***,we prepared disordered electrospun fibers outside the aligned fibers and helical melt-spun fibers on the outer wall of the electrospun fiber *** presence of aligned fibers on the inner surface can promote the extension of nerve cells along the *** helical melt-spun fibers on the outer surface can enhance resistance to kinking and compression and provide *** novel conduit promoted nerve regeneration and functional recovery in a rat sciatic nerve defect model,suggesting that it has potential for clinical use in human nerve injuries.

关键词： aligned fibers anti-kinking helical fibers nerve guidance conduit nerve regeneration peripheral nerve injury topological guidance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bulging Performance and Quality Control of Aluminum Alloy Tailor-welded Overlapping Sheets Based on Interface Friction

引用

Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 258-264页

作者： GAO Tiejun GAO Bowen LI Weijie zhang Jiabin College of Aerospace Engineering Shenyang Aerospace UniversityShenyang 110136China Shenyang Aerospace Xinguang Group Co.L td. Shenyang 110043China

In order to solve the problem of poor formability caused by different materials and properties in the process of tailor-welded sheets forming,a forming method was proposed to change the stress state of tailor-welded sheets by covering the tailor-welded sheets with better plastic properties overlapping *** the same time,the interface friction effect between the overlapping and tailor-welded sheets was utilized to control the stress magnitude and further improve the formability and quality of the tailor-welded *** this work,the bulging process of the tailor-welded overlapping sheets was taken as the research *** alloy tailor-welded overlapping sheets bulging specimens were studied by a combination of finite element analysis and experimental *** results show that the appropriate use of interface friction between tailor-welded and overlapping sheets can improve the formability of tailor-welded sheets and control the flow of weld seam to improve the forming *** increasing the interface friction coefficient on the side of tailor-welded sheets with higher strength and decreasing that on the side of tailor-welded sheets with lower strength,the deformation of the tailor-welded sheets are more uniform,the offset of the weld seam is minimal,the limit bulging height is maximal,and the forming quality is optimal.

关键词： tailor-welded sheets overlapping sheet bulging interface friction weld seam stress

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A Cross-Multi-Domain Trust Assessment Authority Delegation Method Based on Automotive Industry Chain

引用

Computers, Materials & Continua 2025年第1期82卷 407-426页

作者： Binyong Li Liangming Deng Jie zhang Xianhui Deng School of Cybersecurity(Xin Gu Industrial College) Chengdu University of Information TechnologyChengdu610225China Advanced Cryptography and System Security Key Laboratory of Sichuan Province Chengdu610225China SUGON Industrial Control and Security Center Chengdu610225China

To solve the challenges of connecting and coordinating multiple platforms in the automotive industry and to enhance collaboration among different participants,this research focuses on addressing the complex supply relationships in the automotive market,improving data sharing and interactions across various platforms,and achieving more detailed integration of data and *** propose a trust evaluation permission delegation method based on the automotive industry *** proposed method combines smart contracts with trust evaluation mechanisms,dynamically calculating the trust value of users based on the historical behavior of the delegated entity,network environment,and other factors to avoid malicious node attacks during the permission delegation *** also introduce strict control over the cross-domain permission granting and revocation mechanisms to manage the delegation path,prevent information leakage caused by malicious node interception,and effectively protect data integrity and *** analysis shows that this method meets the realtime requirements of collaborative interaction in the automotive industry chain and provides a feasible solution to permission delegation issues in the automotive industry chain,offering dynamic flexibility in authorization and scalability compared to most existing solutions.

关键词： Automotive industry chain cross-domain access block chain delegate authority trust assessment

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A Generative Model-Based Network Framework for Ecological Data Reconstruction

引用

Computers, Materials & Continua 2025年第1期82卷 929-948页

作者： Shuqiao Liu Zhao zhang Hongyan Zhou Xuebo Chen School of Electronic and Information Engineering University of Science and Technology LiaoningAnshan114051China School of Computer Science and Software Engineering University of Science and Technology LiaoningAnshan114051China

This study examines the effectiveness of artificial intelligence techniques in generating high-quality environmental data for species introductory site selection *** Strengths,Weaknesses,Opportunities,Threats(SWOT)analysis data with Variation Autoencoder(VAE)and Generative AdversarialNetwork(GAN)the network framework model(SAE-GAN),is proposed for environmental data *** model combines two popular generative models,GAN and VAE,to generate features conditional on categorical data embedding after SWOT *** model is capable of generating features that resemble real feature distributions and adding sample factors to more accurately track individual sample *** data is used to retain more semantic information to generate *** model was applied to species in Southern California,USA,citing SWOT analysis data to train the *** show that the model is capable of integrating data from more comprehensive analyses than traditional methods and generating high-quality reconstructed data from them,effectively solving the problem of insufficient data collection in development *** model is further validated by the Technique for Order Preference by Similarity to an Ideal Solution(TOPSIS)classification assessment commonly used in the environmental data *** study provides a reliable and rich source of training data for species introduction site selection systems and makes a significant contribution to ecological and sustainable development.

关键词： Convolutional Neural Network(CNN) VAE GAN TOPSIS data reconstruction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Lightweight Underwater Target Detection Using YOLOv8 with Multi-Scale Cross-Channel Attention

引用

Computers, Materials & Continua 2025年第1期82卷 713-727页

作者： Xueyan Ding Xiyu Chen Jiaxin Wang Jianxin zhang School of Computer Science and Engineering Dalian Minzu UniversityDalian116600China Research Center of Multimodal Information Perception and Intelligent Processing Dalian Minzu UniversityDalian116600China

Underwater target detection is extensively applied in domains such as underwater search and rescue,environmental monitoring,and marine resource *** is crucial in enabling autonomous underwater robot operations and promoting ocean ***,low imaging quality,harsh underwater environments,and obscured objects considerably increase the difficulty of detecting underwater targets,making it difficult for current detection methods to achieve optimal *** order to enhance underwater object perception and improve target detection precision,we propose a lightweight underwater target detection method using You Only Look Once(YOLO)v8 with multi-scale cross-channel attention(MSCCA),named *** the proposed multiscale cross-channel attention module,multi-scale attention(MSA)augments the variety of attentional perception by extracting information from innately diverse sensory *** cross-channel strategy utilizes RepVGGbased channel shuffling(RCS)and one-shot aggregation(OSA)to rearrange feature map channels according to specific *** aggregates all features only once in the final feature mapping,resulting in the extraction of more comprehensive and valuable feature *** experimental results show that the proposed YOLOv8-UOD achieves a mAP50 of 95.67%and FLOPs of 23.8 G on the Underwater Robot Picking Contest 2017(URPC2017)dataset,outperforming other methods in terms of detection precision and computational cost-efficiency.

关键词： Deep learning underwater target detection attention mechanism

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Deciphering Water Oxidation Catalysts:The Dominant Role of Surface Chemistry over Reconstruction Degree in Activity Promotion

引用

Nano-Micro Letters 2025年第3期17卷 340-354页

作者： Li An Jianyi Li Yuanmiao Sun Jiamin Zhu Justin Zhu Yeow Seow Hong zhang Nan zhang Pinxian Xi * Zhichuan JXu Chun‑Hua Yan State Key Laboratory of Applied Organic Chemistry Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu ProvinceFrontiers Science Center for Rare IsotopesCollege of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou 730000People’s Republic of China School of Materials Science and Engineering Nanyang Technological UniversitySingapore 639798Singapore Faculty of Materials Science and Energy Engineering Institute of Technology for Carbon NeutralityShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055People’s Republic of China Key Laboratory of Electromagnetic Materials and Devices National Center for International Research On Photoelectric and Energy MaterialsSchool of Materials and EnergyElectron Microscopy CenterYunnan UniversityKunming 650091People’s Republic of China State Key Laboratory of Baiyunobo Rare Earth Resource Researches and Comprehensive Utilization Baotou Research Institute of Rare EarthsBaotou 014030People’s Republic of China Beijing National Laboratory for Molecular Sciences State Key Laboratory of Rare Earth Materials Chemistry and ApplicationsPKU‑HKU Joint Laboratory in Rare Earth Materials and Bioinorganic ChemistryPeking UniversityBeijing 100871People’s Republic of China

Water splitting hinges crucially on the availability of electrocatalysts for the oxygen evolution *** surface reconstruction has been widely observed in perovskite catalysts,and the reconstruction degree has been often correlated with the activity ***,a systematic study on the roles of Fe substitution in activation of perovskite LaNiO_(3)is *** substituting Fe content influences both current change tendency and surface reconstruction ***_(0.9)Fe_(0.1)O_(3)is found exhibiting a volcano-peak intrinsic activity in both pristine and reconstructed among all substituted perovskites in the LaNi_(1-x)Fe_(x)O_(3)(x=0.00,0.10,0.25,0.50,0.75,1.00)*** reconstructed LaNi_(0.9)Fe_(0.1)O_(3)shows a higher intrinsic activity than most reported NiFe-based ***,density functional theory calculations reveal that Fe substitution can lower the O 2p level,which thus stabilize lattice oxygen in LaNi0.9Fe0.1O3 and ensure its long-term ***,it is vital interesting that activity of the reconstructed catalysts relied more on the surface chemistry rather than the reconstruction *** effect of Fe on the degree of surface reconstruction of the perovskite is decoupled from that on its activity enhancement after surface *** finding showcases the importance to customize the surface chemistry of reconstructed catalysts for water oxidation.

关键词： Oxygen evolution reaction Perovskite oxides Doping Activation and reconstruction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：