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卵巢癌中血管生成相关免疫基因预后模型的构建和肿瘤微环境分析

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中国肿瘤生物治疗杂志 2024年第8期31卷 803-814页

作者：吕微王佳丽刘天旭王郁刘丽华河北医科大学第四医院肿瘤免疫治疗科河北石家庄050000 河北省肿瘤研究所河北石家庄050000 河北医科大学国际合作研究干细胞实验室河北石家庄050000

目的:采用生物信息学方法探索卵巢癌中与血管生成相关的免疫基因(ARIG),探讨其与卵巢癌患者预后的关系,并说明不同预后患者肿瘤微环境和免疫治疗的潜在差异,为卵巢癌患者提供新的治疗靶点。方法:分别从TCGA和GEO数据库下载卵巢癌的转录... 详细信息

目的:采用生物信息学方法探索卵巢癌中与血管生成相关的免疫基因(ARIG),探讨其与卵巢癌患者预后的关系,并说明不同预后患者肿瘤微环境和免疫治疗的潜在差异,为卵巢癌患者提供新的治疗靶点。方法:分别从TCGA和GEO数据库下载卵巢癌的转录组数据和生存数据。利用R软件分析差异表达基因,利用Pearson相关系数鉴定血管生成相关基因与免疫相关基因之间的相关性,筛选出差异表达的ARIG。通过Lasso回归分析构建预后模型,通过Cox分析临床特征和风险评分,将样本分为高风险组和低风险组。通过单样本基因集富集分析(ssGSEA)、肿瘤免疫功能障碍和排斥(TIDE)分析预后风险模型与免疫浸润、免疫治疗反应的相关性。最后,收集河北医科大学第四医院2015年5月至2016年5月手术的卵巢癌患者的肿瘤组织和输卵管组织85对,通过qPCR和WB法验证五个差异表达的ARIG在卵巢癌组织中的表达情况,分析其与卵巢癌患者临床病理特征的关系,并初步探索其在卵巢癌细胞的生物学功能。结果:通过生信分析筛选出142个差异表达的ARIG,通过Lasso和Cox回归分析,得到5个基因作为预后基因(PTGER3、SCTR、IGHG1、HSPA8、IGF2),构建了预后风险模型,高风险组患者的预后更差;此外,不同风险评分的患者在免疫细胞浸润和免疫治疗反应方面存在显著差异(均P<0.05)。通过qPCR和WB法验证这5个基因在卵巢癌组织中均为高表达(均P<0.01),其中HSPA8表达量最高,且高表达HSPA8与卵巢癌患者FIGO分期晚、组织分级差、淋巴结转移及腹膜转移呈显著正相关(P<0.001)。细胞功能实验证实,HSPA8可促进卵巢癌细胞的增殖、迁移和侵袭(P<0.01)。结论:差异表达的5种ARIG能有效预测卵巢癌患者的预后,并且与免疫细胞浸润和免疫治疗疗效有关,初步证实其在卵巢中发挥促癌作用。

关键词：卵巢癌血管生成免疫治疗预后肿瘤微环境

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面向尺寸模式保护的高效对称可搜索加密方案

信息网络安全 2024年第6期24卷 843-854页

作者：李强沈援海刘天旭黄晏瑜孙建国西安电子科技大学计算机科学与技术学院西安710071 中移(杭州)信息技术有限公司杭州310023 西安电子科技大学杭州研究院杭州311231

近年来,随着云服务的普及以及对数据安全保护需求的增加,动态对称可搜索加密(DSSE)由于可以在加密数据库中进行更新和查询的特点而受到学界广泛关注。由于考虑搜索和更新效率,DSSE通常需要泄露一些信息,主要包括搜索模式、访问模式和尺... 详细信息

近年来,随着云服务的普及以及对数据安全保护需求的增加,动态对称可搜索加密(DSSE)由于可以在加密数据库中进行更新和查询的特点而受到学界广泛关注。由于考虑搜索和更新效率,DSSE通常需要泄露一些信息,主要包括搜索模式、访问模式和尺寸模式。目前,主要使用不经意随机访问机(ORAM)来保护搜索模式和访问模式,但ORAM无法保证尺寸模式泄露的安全性。文章说明了尺寸模式泄露的危害,并基于现有DSSE隐私概念提出了强前后向隐私,以达到对尺寸模式的保护目的。基于这种增强的安全性目标,文章提出了一种面向尺寸模式保护的高效对称可搜索加密方案Eurus,旨在解决现有方案中的尺寸模式泄露问题。Eurus通过结合多服务器ORAM架构、更新槽机制和细树路径淘汰技术,提供了强正向和反向隐私保护,防止敏感关键字和文件信息被泄露。多服务器ORAM隐藏了搜索和访问模式,更新槽机制混淆了文件标识符,细树路径淘汰技术打乱了文件的实际排列顺序。实验结果表明,Eurus在实现隐私保护的同时,提高了搜索和更新效率,搜索性能较现有方案提升了约46%,更新性能提升了4.73倍。

关键词：可搜索加密尺寸模式 ORAM

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北方寒区肉牛养殖环境控制技术研究进展

黑龙江畜牧兽医 2024年第15期 14-18页

作者：刘天旭周一晨赵贺孙芳陈昭辉中国农业大学动物科学技术学院畜禽营养与饲养全国重点实验室北京100193 勃利县现代农业产业园服务中心黑龙江勃利154500 黑龙江省农业科学院畜牧研究所哈尔滨150086

肉牛养殖过程中的饲养环境与肉牛的生长性能密切相关,因此对养殖环境进行调控有重要意义。目前,肉牛养殖的环境控制主要包括温湿度控制、粪污臭气控制、采光控制及外周围护结构选择等。北方寒区气候寒冷干燥,养殖环境控制相较于其他区... 详细信息

肉牛养殖过程中的饲养环境与肉牛的生长性能密切相关,因此对养殖环境进行调控有重要意义。目前,肉牛养殖的环境控制主要包括温湿度控制、粪污臭气控制、采光控制及外周围护结构选择等。北方寒区气候寒冷干燥,养殖环境控制相较于其他区域具有一定特殊性。文章总结了当前寒区肉牛养殖环境控制技术优缺点及研究进展,以期为环境控制技术的发展提供新的思路,为提高肉牛养殖效率提供新的策略。

关键词：肉牛养殖饲养环境控制技术北方寒区生长性能

1d3v PIC/MCC simulation of dielectric barrier discharge dynamics in hydrogen sulfide

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Plasma Science and Technology 2022年第2期24卷 62-72页

作者： Jing ZHANG Shurong YE tianxu liu Anbang SUN State Key Laboratory of Safety and Control for Chemicals SINOPEC Research Institute of Safety Engineering Co.Ltd.Qingdao 266071People's Republic of China State Key Laboratory of Electrical Insulation and Power Equipment School of Electrical EngineeringXi'an Jiaotong UniversityXi'an 710049People's Republic of China

In this study,we computationally examined the dynamics of dielectric barrier discharge in hydrogen *** simulations were performed with a 1 d3 v particle-in-cell/Monte Carlo collision model in which a parallel-plate electrode geometry with dielectrics was *** recombination process is represented in the *** discharge mode was found to be initially Townsend discharge developing from the cathode to the anode,and at the peak of the current,a more stable glow discharge develops from the anode to the cathode.A higher applied voltage results in sufficient secondary electrons to trigger a second current peak,and then the current amplitude *** the frequency is increased,it leads to the advance of the phase and an increase in the amplitude of the current peak.A higher dielectric permittivity also makes the discharge occur earlier and more violently in the gap.

关键词： plasma simulation dielectric barrier discharge PIC simulation

Large-area ultrastrong and stiff aramid nanofiber based layered nanocomposite films

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Nano Research 2024年第2期17卷 829-835页

作者： Zhidong Nie Yingzhi Sun tianxu Zhou Shengwen Kong Linxing liu Chuangqi Zhao Mingjie liu Key Laboratory of Bioinspired Smart Interfacial Science and Technology of Ministry of Education School of ChemistryBeihang UniversityBeijing 100191China University of Science and Technology of China Hefei 230026China Suzhou Institute for Advanced Research University of Science and Technology of ChinaSuzhou 215123China International Research Institute for Multidisciplinary Science Beihang UniversityBeijing 100191China

One-dimensional(1D)aramid nanofiber(ANF)based nanocomposite films have drawn increasing attentions in various applications due to their excellent mechanical properties and impressive chemical and thermal ***,the large-area fabrication of aramid nanocomposite films with ultrastrong mechanical properties under mild conditions remains a great *** we present a facile superspreading-assisted strategy to produce aramid nanofiber based oriented layered nanocomposites using phase inversion process that occurs at the fully swollen hydrogel *** nanocomposite films based on ANF,carboxylation carbon tube(CNT–COOH),poly(vinyl alcohol)(PVA),and MXene nanosheet exhibit a tensile strength of up to 870.8±85 MPa,a Young’s modulus of 21.8±2.2 GPa,and outstanding toughness(up to 43.2±4.6 MJ/m^(3)),which are much better than those conventional aramid nanofiber based *** conductivity of our nanocomposite films reaches the maximum of about 1100 S/*** fabulous mechanical properties combination and continuous production capability render our strategy representing a promising direction for the development of high-performance nanocomposites.

关键词： aramid nanofibers MXene nanosheets nanocomposites ultrastrong and stiff superspreading large-area production

Egg cell-secreted aspartic proteases ECS1/2 promote gamete attachment to prioritize the fertilization of egg cells over central cells in Arabidopsis

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Journal of Integrative Plant Biology 2022年第11期64卷 2047-2059页

作者： Jiahao Jiang Nils Stührwohldt tianxu liu Qingpei Huang Ling Li Li Zhang Hongya Gu liumin Fan Sheng Zhong Andreas Schaller Li-Jia Qu State Key Laboratory for Protein and Plant Gene Research Peking-Tsinghua Center for Life Sciences at College of Life SciencesPeking UniversityBeijing 100871China Department of Plant Physiology and Biochemistry Institute of BiologyUniversity of HohenheimStuttgart 70593Germany

Double fertilization is an innovative phenomenon in angiosperms,in which one sperm cell first fuses with the egg cell to produce the embryo,and then the other sperm fuses with the central cell to produce the ***,the molecular mechanism of the preferential fertilization of egg cells is poorly *** this study,we report that two egg cell-secreted aspartic proteases,ECS1 and ECS2,play an important role in promoting preferential fertilization of egg cells in *** show that simultaneous loss of ECS1 and ECS2 function resulted in an approximately 20%reduction in fertility,which can be complemented by the full-length ECS1/2 but not by corresponding active site mutants or by secretion-defective versions of ECS1/*** phenotypic analysis revealed that the egg cellsperm cell attachment was compromised in ecs1 ecs2 *** pollination assays with cyclin-dependent kinase a1(cdka;1)pollen showed that preferential egg cell fertilization was impaired in the ecs1 ecs2 *** together,these results demonstrate that egg cells secret two aspartic proteases,ECS1 and ECS2,to facilitate the attachment of sperm cells to egg cells so that preferential fertilization of egg cells is *** study reveals the molecular mechanism of preferential fertilization in Arabidopsis thaliana.

关键词： Arabidopsis thaliana double fertilization ECS1/2 gamete interaction preferential fertilization

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结构可靠性拓扑优化的深度学习协同方法

宁波大学学报（理工版） 2024年第4期37卷 103-109页

作者：阮相当许孟辉李萍蒋章宇贺元强刘天旭陶磊宁波大学机械工程与力学学院浙江宁波315211 新疆理工学院理学院新疆阿克苏843100

在给定约束条件下,结构拓扑优化以最优化性能获得设计域内材料的最佳布局.传统拓扑优化面临两大挑战:其一,未定量计入参数不确定性的影响,导致优化后结构在服役中常常不满足性能约束;其二,基于有限元模型的拓扑优化分辨率严重依赖于有... 详细信息

在给定约束条件下,结构拓扑优化以最优化性能获得设计域内材料的最佳布局.传统拓扑优化面临两大挑战:其一,未定量计入参数不确定性的影响,导致优化后结构在服役中常常不满足性能约束;其二,基于有限元模型的拓扑优化分辨率严重依赖于有限元网格.本文以随机参数定量计及固有不确定性,基于顺序优化与可靠性分析实现可靠性优化模型的确定性转化.进一步,基于网络训练与拓扑优化的过程相似性,以深度神经网络表达单元相对密度与网络参数之间的映射,通过构造计及可靠性的损失函数,将拓扑优化过程转换为网络训练过程.算例验证了所提方法获得的拓扑优化结构能够在满足性能可靠性要求的前提下实现较高的拓扑分辨率.

关键词：拓扑优化可靠性优化深度神经网络有限元分析

Transdermal treatment for malignant melanoma by aptamer-modified tetrahedral framework nucleic acid delivery of vemurafenib

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Chinese Chemical Letters 2024年第4期35卷 260-267页

作者： Dexuan Xiao Tianyu Chen tianxu Zhang Sirong Shi Mei Zhang Xin Qin Yunkun liu Longjiang Li Yunfeng Lin State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesWest China Hospital of StomatologySichuan UniversityChengdu 610041China Sichuan Provincial Engineering Research Center of Oral Biomaterials Chengdu 610041China Department of Head and Neck Oncology National Clinical Research Center for Oral DiseasesWest China Hospital of StomatologySichuan UniversityChengdu 610041China

Melanoma is one of the most malignant skin tumors, whose high invasion is generally associated with BRAF gene mutation. Although new chemotherapeutic drugs, such as vemurafenib, have been developed to inhibit the growth of melanoma, these drugs are usually administered intravenously or orally, resulting in toxic side effects on major tissues and organs. Tetrahedral framework nucleic acids(tFNAs) are a novel type of DNA nanostructures with excellent biocompatibility and versatility which have been proven to penetrate through skin barrier with ease. In this study, we prepared t FNAs with vemurafenib and connected DNA aptamer AS1411 at the apex of t FNAs(AS1411-tFNAs/vemurafenib). On one hand, AS1411-tFNAs/vemurafenib could kill melanoma cells by blocking the mutated BRAF gene in vitro. Compared with free vemurafenib, AS1411-tFNAs/vemurafenib had no obvious toxicity to normal cells. On the other hand,AS1411-tFNAs could transfer vemurafenib to cross through the skin barrier and permeate into tumor tissues. In vivo, transdermal delivery of AS1411-t FNAs/vemurafenib could inhibit the growth of human A375melanoma, whose inhibiting effect was stronger than intravenous administration of vemurafenib. These results demonstrated the application prospects of tFNAs combined with chemotherapeutic drugs in skin tumors.

关键词： Melanoma Vemurafenib DNA nanostructure Transdermal treatment Side effect

当代西方学界的脆弱国家政治话语纷争及其启示

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江汉论坛 2022年第1期 43-50页

作者：刘天旭三峡大学马克思主义学院湖北宜昌443002

进入21世纪以来,西方脆弱国家政治话语的全球传播大致分为概念酝酿、快速兴起、走向衰退三个阶段。在此过程中,西方学界在脆弱国家概念的内涵、衡量脆弱性的指标以及跳出脆弱性陷阱的有效路径等方面存在着持续不断的理论纷争。争论的原... 详细信息

进入21世纪以来,西方脆弱国家政治话语的全球传播大致分为概念酝酿、快速兴起、走向衰退三个阶段。在此过程中,西方学界在脆弱国家概念的内涵、衡量脆弱性的指标以及跳出脆弱性陷阱的有效路径等方面存在着持续不断的理论纷争。争论的原因,虽有国家利益的分歧,不过就学术层面而言,却是任何政治话语体系有效传播必不可少的内在发展机制使然。当代西方的脆弱国家政治话语纷争,对于有效提升中国的国际话语权具有一定的启示价值。

关键词：政治话语话语纷争脆弱国家

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