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Label-free Fluorescent DNA Biosensors Based on Metallointercalators and Nanomaterials

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Label-free Fluorescent DNA Biosensors Based on Metallointerc...

第八届全国化学生物学学术会议

作者： Shuo Shi tianming yao Liangnian Ji Department of Chemistry Tongji University MOE Laboratory of Bioinorganic and Synthetic Chemistry School of Chemistry and Chemical EngineeringSun Yat-Sen University

＜正＞DNA based biosensors have become increasingly important in medical diagnostics, genetic and drug screening,and forensics in this post-genomic *** labeling methods suffer from several drawbacks,which are tedious,cost-expensive and time-consuming,thus the development of simple and general label free strategies is being *** present article introduces a new model of biosensor devices

关键词： Biosensors Metallointercalators Nanomaterials Label-free Fluorescence

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原位悬浮聚合制备聚苯乙烯/炭黑复合微球

北京化工大学学报（自然科学版） 2016年第5期43卷 34-38页

作者：宁天明姚明北京化工大学材料科学与工程学院碳纤维及功能高分子教育部重点实验室北京100029

以磷酸三钙(TCP)和聚乙烯醇(PVA)为分散剂,过氧化苯甲酰(BPO)为引发剂,二乙烯基苯(DVB)为交联剂,对炭黑与苯乙烯进行原位悬浮聚合,制备了聚苯乙烯/炭黑复合粒子。探讨了分散速度和分散时间对炭黑在苯乙烯中均匀分散的影响,研究了反应过... 详细信息

以磷酸三钙(TCP)和聚乙烯醇(PVA)为分散剂,过氧化苯甲酰(BPO)为引发剂,二乙烯基苯(DVB)为交联剂,对炭黑与苯乙烯进行原位悬浮聚合,制备了聚苯乙烯/炭黑复合粒子。探讨了分散速度和分散时间对炭黑在苯乙烯中均匀分散的影响,研究了反应过程中温度的控制、分散剂的用量对聚合的影响。实验结果表明:利用高速分散机分散,分散速度越大,所需分散时间越短;当选用有机-无机复合分散剂磷酸三钙(TCP)和聚乙烯醇(PVA),质量比为2∶1,分段反应升温,先在75℃下反应1.5 h,然后缓慢升温至85℃反应4 h,最后在95℃下反应0.5 h,可以得到成球率100%、粒径较为理想的黑色有光泽的聚苯乙烯/炭黑复合微球。DSC测试结果表明,在加入炭黑后聚合物的玻璃化转变温度(Tg)提高10℃左右,但炭黑用量(质量分数)从0.1%增加到0.5%时,聚合物Tg并不随炭黑用量的增加而增加。

关键词：炭黑聚苯乙烯复合微球原位聚合悬浮聚合耐热性能

Carbon nanotubes immunosensor for detecting trace quantities of immune globulin

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Carbon nanotubes immunosensor for detecting trace quantities...

中国化学会第28届学术年会

作者： Hao Chen 1,tianming yao 1 Department of Chemistry,Tongji University,Shanghai,200092

＜正＞Point-of-care protein detection for diagnosis of diseases require low cost techniques,while maintaining minimum sample amounts and operational simplicity,detection *** commercial immunology

关键词： carbon nanotube sandwich immunological method IgG protein detection

Clinical feasibility and safety of a novel miniature mobile cardiaccatheterization laboratory in diagnosis and treatment for coronaryheart disease

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Chinese Medical Journal 2014年第6期127卷 1052-1056页

作者： Ming Liang Yaling Han Geng Wang tianming yao Jingyang Sun Fei Li Kai Xu Bin Wang Yi Li Jingjing Rong Xiaozeng Wang Department of Cardiology Second Affiliated Hospital of Dalian Medical University Dalian Liaoning 116027 China. Department of Cardiology Northern Hospital Shenyang Liaoning 110016 China. Email: hanyl@***. Department of Cardiology Northern Hospital Shenyang Liaoning 110016 China.

Background The lack of medical facilities causes delayed diagnosis and treatment of coronary heart disease in remote mountainous area and/or at disaster site. The miniature mobile cardiac catheterization laboratory was developed to be an intervention platform for coronary heart disease diagnosis and treatment by our team. Pre-clinical research indicated that the miniature mobile cardiac catheterization laboratory performed well in the rescue of critical cardiovascular diseases, even ST-segment elevation myocardial infarction. The present study aimed to evaluate the clinical safety and timeliness of the miniature mobile cardiac catheterization laboratory for emergent coronary interventional diagnosis and *** X-ray radiation safety and disinfection efficacy in the miniature mobile cardiac catheterization laboratory were tested during working status. Coronary angiography and/or percutaneous coronary intervention were performed in remote mountainous areas on patients who were first diagnosed as having coronary heart disease by senior interventional cardiologists. The percutaneous coronary intervention procedures and results from patients in the miniature mobile cardiac catheterization laboratory were compared with patients who were treated in the hospital catheter *** The X-ray radiation dosages in the miniature mobile cardiac catheterization laboratory were 39.55 μGy/s, 247.4 μGy/h, 90.3 μGy/h and 39.4 μGy/h which were corresponded to 0 m, 1 m, 2 m and 3 m away from the tube central of the medium C-arm. And the radiation dosages used in the miniature mobile cardiac catheterization laboratory were less than the corresponding positions in the hospital catheter lab. The numbers of bacteria colonies in the miniature mobile cardiac catheterization laboratory in different environments range from(60±8) cfu/m3 to(120±10) cfu/m3 and met the demands of percutaneous coronary intervention. A total of 17 patients who received angiography in the miniature mobil

关键词： coronary heart disease diagnosis treatment percutaneous coronary intervention mobile catheter lab

Highly Sensitive Immunoassay by Fluorescence with Gold Nanoparticles

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Highly Sensitive Immunoassay by Fluorescence with Gold Nanop...

中国化学会第28届学术年会

作者： Tingting Cheng 1,tianming yao 1,1 Department of Chemistry,Tongji University,Shanghai,200092

＜正＞Highly sensitive measurement of bioactive molecules,such as disease-relating proteins or tumor markers,is a critical need in modern biochemistry and clinical diagnosis,while some commercial

关键词： immunity gold nanorods IgG trace detection

The Comparison of Remedy Vascular Injury with Different Emboliaztion Agents in the Comprehensive Surgical Treatment Shelter

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The Comparison of Remedy Vascular Injury with Different Embo...

中华医学会第十五次全国心血管病学大会

作者： Shibei Li Ming Liang tianming yao Zulu Wang Yanchun Liang Kai Xu Yaling Han Department of Cardiology Institute of Cardiovascular Research of People’s Liberation Army Shenyang Northern Hospital

Background There is no report about embolic agent used to treat different types of vascular injury in the comprehensive surgical treatment shelter. Methods Producing different types of vascular injury animal models in the comprehensive surgical treatment shelter, using different types of embolic agent such as gelatin sponge, sodium alginate - chitosan

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大环配合物在核酸切割中的应用

化学通报 2008年第10期71卷 731-738页

作者：李凤莲石硕姚天明同济大学化学系上海200092

核酸切割试剂的研究是化学和分子生物学中最为活跃的前沿领域之一。研究核酸切割试剂,不仅是因为对核酸酶催化机理进行深入了解之需,同时还因为人工核酸酶在基因治疗中有着诱人的前景。大环配合物的合成、结构及其对核酸的切割作用已有... 详细信息

核酸切割试剂的研究是化学和分子生物学中最为活跃的前沿领域之一。研究核酸切割试剂,不仅是因为对核酸酶催化机理进行深入了解之需,同时还因为人工核酸酶在基因治疗中有着诱人的前景。大环配合物的合成、结构及其对核酸的切割作用已有广泛的文献报道。本文综述了作为重要的核酸切割试剂的大环多胺配合物和氮杂冠醚配合物对核酸的切割作用及其规律性,并展望了今后的发展与应用方向。

关键词：大环配合物多胺氮杂冠醚核酸切割

The Diagnosis of Vascular Injury by Ultrasonography Combining Angiography in the Comprehensive Surgical Treatment Shelter

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The Diagnosis of Vascular Injury by Ultrasonography Combinin...

中华医学会第十五次全国心血管病学大会

Background There is no report about diagnosis of vascular injury by ultrasonography combining angiography in the comprehensive surgical treatment shelter recently. Methods Producing different site of vascular injury a...

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耦合液膜传输的研究进展

化学进展 2001年第3期13卷 177-182页

作者：傅成武姚天明施宪法施宪法同济大学化学系上海200092

综述了近年来含流动载体的质子。

关键词：液膜耦合传输同向传输反向传输生物膜膜分离电子耦合传输质子耦合传输光耦合传输