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TRIM47 is a novel endothelial activation factor that aggravates lipopolysaccharide-induced acute lung injury in mice via K63-linked ubiquitination of TRAF2

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Signal Transduction and Targeted Therapy 2022年第6期7卷 2041-2052页

作者： Yisong Qian Ziwei Wang Hongru Lin tianhua lei Zhou Zhou Weilu Huang Xuehan Wu Li Zuo Jie Wu Yu Liu Ling-Fang Wang Xiao-Hui Guan Ke-Yu Deng Mingui Fu Hong-Bo Xin The National Engineering Research Center for Bioengineering Drugs and the Technologies Institute of Translational MedicineNanchang University1299 Xuefu RdHonggu District 330031 NanchangChina Department of Biomedical Science School of MedicineUniversity of Missouri Kansas City2411 Holmes StreetKansas CityMO 64108USA

Endothelial activation plays an essential role in the pathogenesis of sepsis-induced acute lung injury,however,the detailed regulatory mechanisms remain largely unknown.Here,we reported that TRIM47,an E3 ubiquitin ligase of the tripartite motifcontaining protein family,was highly expressed in vascular endothelial cells.TRIM47-deficient mice were effectively resistant to lipopolysaccharide(LPS)-induced acute lung injury and death by attenuating pulmonary inflammation.TRIM47 was upregulated during TNFα-induced endothelial activation in vitro.Knockdown of TRIM47 in endothelial cells inhibited the transcription of multiple pro-inflammatory cytokines,reduced monocyte adhesion and the expression of adhesion molecules,and suppressed the secretion of IL-1βand IL-6 in endothelial cells.By contrast,overexpression of TRIM47 promoted inflammatory response and monocyte adhesion upon TNFαstimulation.In addition,TRIM47 was able to activate the NF-κB and MAPK signaling pathways during endothelial activation.Furthermore,our experiments revealed that TRIM47 resulted in endothelial activation by promoting the K63-linked ubiquitination of TRAF2,a key component of the TNFαsignaling pathway.Taken together,our studies demonstrated that TRIM47 as a novel activator of endothelial cells,promoted LPS-induced pulmonary inflammation and acute lung injury through potentiating the K63-linked ubiquitination of TRAF2,which in turn activates NF-κB and MAPK signaling pathways to trigger an inflammatory response in endothelial cells.

关键词： ubiquitin acute inhibited

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TRIM65 E3 ligase targets VCAM-1 degradation to limit LPS-induced lung inflammation

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Journal of Molecular Cell Biology 2020年第3期12卷 190-201页

作者： Yong Li Xuan Huang Fang Guo tianhua lei Shitao Li Paula Monaghan-Nichols Zhisheng jiang Hong-Bo Xin Mingui Fu Department of Biomedical Science School of MedicineUniversity of Missouri-Kansas CityKansas CityMO 64108USA Institute of Translational Medicine Nanchang UniversityNanchang 330031China Institute of Cardiovascular Diseases Department of PathophysiologyHengyang Medical CollegeUniversity of South ChinaHengyang A21001China Department of Physiological Sciences Center for Veterinary and Health sciencesOklahoma State UniversityStillwaterOK 7A078USA

Although the adhesion molecules-mediated leukocyte adherence and infiltration into tissues is an important step of inflammation,the post-translational regulation of these proteins on the endothelial cells is poorly understood.Here,we report that TRIM65,an ubiquitin E3 iigase of tripartite protein family,selectively targets vascular cell adhesion molecule 1(VCAM-1)and promotes its ubiq-uitination and degradation,by which it critically controls the duration and magnitude of sepsis-induced pulmonary inflammation.TRIM65 is constitutively expressed in human vascular endothelial cells.During TNFa-induced endothelial activation,the protein levels ofTRIM65 and VCAM-1 are inversely correlated.Expression of wild-type TRIM65,but not expression of aTRIM65 mutant that lacks E3 ubiquitin ligase function in endothelial cells,promotes VCAM-1 ubiquitination and degradation,whereas small interference RNA-mediated knockdown of TRIM65 attenuates VCAM-1 protein degradation.Further experiments show that TRIM65 directly interacts with VCAM-1 protein and directs its polyubiquitination,by which TRIM65 controls monocyte adherence and infiltration into tissues during inflammation.Importantly,TRIM65-deficient mice are more sensitive to lipopolysaccharide-induced death,due to sustained and severe pulmonary inflammation.Taken together,our studies suggest that TRIM65-mediated degradation of VCAM-1 represents a potential mechanism that controls the duration and magnitude of infiammation.

关键词： TRIM65 VCAM-1 endothelial activation lung inflammation ubiquitination

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基于安全接头的顶驱钻柱转矩无线测量技术研究

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传感器与微系统 2024年第5期43卷 55-57页

作者：闫文辉林天华严磊邵军彭勇西安石油大学机械工程学院陕西西安710000 成都电讯达科技有限公司四川成都610000

钻柱转矩作为反映钻具工作状态的重要参数之一,对其进行转矩测量尤为重要。本文设计了一种基于顶驱安全接头的转矩无线测量系统。以实验试件表面应变等效替代型号为AJ-H203安全接头表面应变,制作了安全接头实验试件,对安全接头和实验试... 详细信息

钻柱转矩作为反映钻具工作状态的重要参数之一,对其进行转矩测量尤为重要。本文设计了一种基于顶驱安全接头的转矩无线测量系统。以实验试件表面应变等效替代型号为AJ-H203安全接头表面应变,制作了安全接头实验试件,对安全接头和实验试件进行了有限元静力学仿真,并完成了实验试件的测量实验,实验灵敏度为9.447 V/(kN·m)。实验灵敏度与理论灵敏度偏差为0.04 V/(kN·m)。表明:实验试件替代安全接头模型进行实验是合理的,说明可以通过安全接头进行顶驱钻柱转矩的测量。

关键词：安全接头顶驱转矩测量等效替代

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基于QbD理念的大肠埃希菌摇瓶阶段培养工艺开发

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中国生物制品学杂志 2024年第6期37卷 710-717页

作者：徐世友白彩虹孙田华易中恒李薇焦磊雷清兰州生物制品研究所有限责任公司、甘肃省疫苗工程技术研究中心甘肃兰州730046

目的基于质量源于设计(Quality by Design,QbD)理念,开发一种具有较高生物量与较高菌体活力培养特点的大肠埃希菌摇瓶阶段培养工艺。方法以不同摇瓶配置为考察因素,菌体悬浮液A600值、培养物湿菌重和菌体活力值为培养结果考察指标,采用... 详细信息

目的基于质量源于设计(Quality by Design,QbD)理念,开发一种具有较高生物量与较高菌体活力培养特点的大肠埃希菌摇瓶阶段培养工艺。方法以不同摇瓶配置为考察因素,菌体悬浮液A600值、培养物湿菌重和菌体活力值为培养结果考察指标,采用方差分析分析培养结果以获得具有高生物量和高菌体活力的第3次扩增摇瓶配置。以摇床温度和摇床转速为试验因子进行2因子2水平全因子试验,菌体悬浮液A值为响应值,培养累计时间为变量因素,摇床实时在线温度与摇床设备自测转速为补充变量因素,采用函数型主成分分析(functional principal component analysis,FPCA)方法进行广义回归,拟合生长曲线模型,通过生长曲线模型获得优化后的培养停止时间及培养工艺,对响应值添加随机噪声蒙特卡洛模拟(Monte Carlo simulation,MCS),再次优化获得摇床培养工艺设计空间,选择设计空间中最劣条件作为验证试验设定条件,以不同培养停止时间分阶段进行连续10批次验证试验。结果第3次扩增摇瓶配置:5 L一次性高效摇瓶,大面积透气膜盖。培养工艺设计空间:摇床温度36.5~37.5℃,摇床转速220~230 r/min,设计培养停止时间18 h。最劣条件验证试验表明,停止培养时间为16 h时,可获得较高菌体活力值且较低生物量的培养结果,停止培养时间为18 h时,可获得较高水平的生物量及菌体活力值。结论本研究设计的大肠埃希菌摇瓶阶段培养工艺具有较高生物量及较高菌体活力培养特点,同时可依据此培养工艺适应性调整以满足不同培养需求。

关键词：大肠埃希菌培养工艺摇瓶方差分析函数型主成分分析质量源于设计

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不翻瓣技术拔除下颌中位水平阻生智齿对患者术后生活质量的影响研究

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中国美容医学 2024年第4期33卷 120-123页

作者：刘苗雷冰王昱新姚天华西安交通大学口腔医院、陕西省颅颌面精准医学研究重点实验室、陕西省牙颌疾病临床研究中心、西安交通大学口腔医院口腔综合科陕西西安710000

目的:研究不翻瓣技术拔除下颌中位水平阻生智齿对患者生活质量的影响。方法:选取2022年6月-2023年2月就诊于笔者医院口腔综合科的410例下颌中位水平阻生智齿患者,随机分为观察组和对照组,其中观察组207例,对照组203例。观察组采用不翻... 详细信息

目的:研究不翻瓣技术拔除下颌中位水平阻生智齿对患者生活质量的影响。方法:选取2022年6月-2023年2月就诊于笔者医院口腔综合科的410例下颌中位水平阻生智齿患者,随机分为观察组和对照组,其中观察组207例,对照组203例。观察组采用不翻瓣技术,对照组采用传统翻瓣技术。比较两组术后出血、疼痛、肿胀、感染、张口受限、干槽症的发生情况以及PoSSe评分比较,采用SPSS21.0进行统计学分析。结果:观察组术后出血、疼痛、肿胀、张口受限的发生率均高于对照组(P<0.05);观察组PoSSe评分(71.24±5.01)分,高于对照组的(52.09±9.48)分(P<0.05)。结论:不翻瓣技术用于拔除下颌中位水平阻生智齿时可以减轻患者术后疼痛、开口受限、面颊部肿胀等并发症,宜临床推广应用。

关键词：不翻瓣技术下颌阻生智齿并发症术后生活质量

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Highly efficient acousto-optic modulation using nonsuspended thin-fil1m lithium niobate-chalcogenide hybrid waveguides

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Light(Science & Applications) 2022年第6期11卷 1285-1295页

作者： lei Wan Zhiqiang Yang Wenfeng Zhou Meixun Wen tianhua Feng Siqing Zeng Dong Liu Huan Li Jingshun Ppan Ning Zhu Weiping Liu Zhaohui Li Department of Electronic Engineering College of Information Science and TechnologyJinan University510632 GuangzhouChina Guangdong Provincial Key Laboratory of Optoelectronic Information Processing Chips and Systems Sun Yat-sen University510275 GuangzhouChina State Key Laboratory for Modern Optical Instrumentation College of Optical Science and EngineeringInternational Research Center for Advanced PhotonicsZhejiang UniversityZijingang Campus310058 HangzhouChina Institute of Semiconductor Science and Technology Guangdong Engineering Technology Research Center of Low Carbon and New Energy MaterialsSouth China Normal University510631 GuangzhouChina Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) 519000 ZhuhaiChina

A highly efficient on-chip acousto-optic modulator is as a key component and occupies an exceptional position in microwave-to-optical conversion.Homogeneous thin-film lithium niobate is preferentially employed to build the suspended configuration for the acoustic resonant cavity,with the aim of improving the modulation efficiency of the device.However,the limited cavity length and complex fabrication recipe of the suspended prototype restrain further breakthroughs in modulation efficiency and impose challenges for waveguide fabrication.In this work,based on a nonsuspended thin-film lithium niobate-chalcogenide glass hybrid Mach-Zehnder interferometer waveguide platform,we propose and demonstrate a built-in push-pull acousto-optic modulator with a half-wave-voltage-length product VnL as low as 0.03 V cm that presents a modulation efficiency comparable to that of a state-of-the-art suspended counterpart.A microwave modulation link is demonstrated using our developed built-in push-pull acousto-optic modulator,which has the advantage of low power consumption.The nontrivial acousto-optic modulation performance benefits from the superior photoelastic property of the chalcogenide membrane and the completely bidirectional participation of the antisymmetric Rayleigh surface acoustic wave mode excited by the impedance-matched interdigital transducer,overcoming the issue of low modulation efficiency induced by the incoordinate energy attenuation of acoustic waves applied to the Mach-Zehnder interferometer with two arms in traditional push-pull acousto-optic modulators.

关键词： modulator waveguide interferometer

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结核分枝杆菌Rv2626c蛋白的原核表达、纯化及抗原活性鉴定

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中国热带医学 2024年第4期24卷 472-477页

作者：张光磊孙田华吴智远张婷婷胡丽娜王婷李会蒋保余李朋伟焦磊兰州生物制品研究所有限责任公司第三研究室甘肃省疫苗工程技术研究中心甘肃兰州730046

目的构建结核分枝杆菌Rv2626c蛋白的重组表达载体,在大肠埃希菌(Escherichia coli,E.coli)中表达、纯化后研究Rv2626c重组蛋白的抗原性。方法将GenBank公布的结核分枝杆菌H37Rv株Rv2626c蛋白的氨基酸序列(登录号:CCP45424.1)按E.coli密... 详细信息

目的构建结核分枝杆菌Rv2626c蛋白的重组表达载体,在大肠埃希菌(Escherichia coli,E.coli)中表达、纯化后研究Rv2626c重组蛋白的抗原性。方法将GenBank公布的结核分枝杆菌H37Rv株Rv2626c蛋白的氨基酸序列(登录号:CCP45424.1)按E.coli密码子偏好转换为对应的DNA序列,合成该DNA序列并克隆至pET24a(+)质粒上,构建pET24a(+)-Rv2626c重组质粒,转化至E.coli BL21(DE3)中,以不同的异丙基-β-D-硫代半乳糖苷(isopropylβ-D-thiogalactopyranoside,IPTG)浓度、温度和时间条件下诱导表达Rv2626c蛋白,通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(sodium dodecyl sulfate-polyacrylamide gel electrophoresis,SDS-PAGE)和蛋白免疫印迹法(Western Blot)对Rv2626c重组蛋白进行鉴定。利用镍螯合亲和层析法纯化Rv2626c重组蛋白并将其免疫青紫蓝兔,制备抗Rv2626c抗血清,采用Western Blot和酶联免疫吸附法分别检测抗血清的特异性和效价。结果pET24a(+)-Rv2626c重组质粒构建成功。SDS-PAGE检测显示:转入该重组质粒的E.coli经IPTG诱导后表达Rv2626c重组蛋白,分子量约14500,大小与预期相符;Rv2626c重组蛋白的最佳诱导表达条件为31℃1.0 mmol/L IPTG诱导表达6 h,目的蛋白主要以可溶性形式存在,与Western Blot检测结果一致。Rv2626c重组蛋白免疫青紫蓝兔获得的高免血清特异性良好,经酶联免疫吸附法测定该血清效价为1∶256000。结论在E.coli中成功表达结核分枝杆菌Rv2626c蛋白,且纯化的Rv2626c重组蛋白具有较好的纯度和抗原活性,为进一步揭示其生物学功能奠定基础。

关键词：结核分枝杆菌 Rv2626c重组蛋白抗原活性鉴定

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Altered brain activity in juvenile myoclonic epilepsy with a monotherapy:a resting-state fMRI study

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Acta Epileptologica 2022年第3期5卷 186-193页

作者： Linyuan Qin Yingying Zhang Jiechuan Ren Du lei Xiuli Li tianhua Yang Qiyong Gong Dong Zhou Department of Neurology West China HospitalSichuan UniversityChengdu 610041China Department of Neurology Beijing Tiantan HospitalCapital Medical UniversityBeijing 100070China Department of Radiology Huaxi MR Research CenterWest China HospitalSichuan UniversityChengdu 610041China

Background:Juvenile myoclonic epilepsy(JME)is the most common syndrome of idiopathic generalized epilepsy.Although resting-state functional magnetic resonance imaging(rs-fMRI)studies have found thalamocortical circuit dysfunction in patients with JME,the pathophysiological mechanism of JME remains unclear.In this study,we used three complementary parameters of rs-fMRI to investigate aberrant brain activity in JME patients in comparison to that of healthy controls.Methods:Rs-fMRI and clinical data were acquired from 49 patients with JME undergoing monotherapy and 44 ageand sex-matched healthy controls.After fMRI data preprocessing,the fractional amplitude of low-frequency fluctuation(fALFF),regional homogeneity(ReHo),and degree centrality(DC)were calculated and compared between the two groups.Correlation analysis was conducted to explore the relationship between local brain abnormalities and clinical features in JME patients.Results:Compared with the controls,the JME patients exhibited significantly decreased fALFF,ReHo and DC in the cerebellum,inferior parietal lobe,and visual cortex(including the fusiform and the lingual and middle occipital gyri),and increased DC in the right orbitofrontal cortex.In the JME patients,there were no regions with reduced ReHo compared to the controls.No significant correlation was observed between regional abnormalities of fALFF,ReHo or DC,and clinical features.Conclusions:We demonstrated a wide range of abnormal functional activity in the brains of patients with JME,including the prefrontal cortex,visual cortex,default mode network,and cerebellum.The results suggest dysfunctions of the cerebello-cerebral circuits,which provide a clue on the potential pathogenesis of JME.

关键词： Juvenile myoclonic epilepsy Resting-state functional magnetic resonance imaging Fractional amplitude of low-frequency fluctuation Regional homogeneity Degree centrality

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A review of dielectric optical metasurfaces for spatial differentiation and edge detection

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Frontiers of Optoelectronics 2021年第2期14卷 187-200页

作者： lei WAN Danping PAN tianhua FENG Weiping LIU Alexander A.POTAPOV Department of Electronic Engineering College of Information Science and TechnologyJinan UniversityGuangzhou 510632China Wuhan National Laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhan 430074China JNU-IREE RAS Joint Laboratory of Information Techniques and Fractal Signal Processing Jinan UniversityGuangzhou 510632China Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Education Institutes Jinan UniversityGuangzhou 510632China Institute of Radio Engineering and Electronics Russian Academy of SciencesMoscow 125009Russia

Dielectric metasurfaces-based planar optical spatial differentiator and edge detection have recently been proposed to play an important role in the parallel and fast image processing technology.With the development of dielectric metasurfaces of different geometries and resonance mechanisms,diverse on-chip spatial differentiators have been proposed by tailoring the dispersion characteristics of subwavelength structures.This review focuses on the basic principles and characteristic parameters of dielectric metasurfaces as first-and second-order spatial differentiators realized via the Green's function approach.The spatial bandwidth and polarization dependence are emphasized as key properties by comparing the optical transfer flinctions of metasurfaces for different incident wavevectors and polarizations.To present the operational capabilities of a two-dimensional spatial differentiator in image information acquisition,edge detection is described to illustrate the practicability of the device.As an application example,experimental demonstrations of edge detection for different biological cells and a flower mold are discussed,in which a spatial differentiator and objective lens or camera are integrated in three optical pathway configurations.The realization of spatial differentiators and edge detection with dielectric metasurfaces provides new opportunities for ultrafast information identification in biological imaging and machine vision.

关键词： dielectric metasurfaces spatial differentiator edge detection optical transfer function

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Central role of myeloid MCPIP1 in protecting against LPSinduced inflammation and lung injury

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Signal Transduction and Targeted Therapy 2017年第1期2卷 18-32页

作者： Yong Li Xuan Huang Shengping Huang Hui He tianhua lei Fatma Saaoud Xiao-Qiang Yu Ari Melnick Anil Kumar Christopher J Papasian Daping Fan Mingui Fu Department of Biomedical Science and Shock/Trauma Research Center School of MedicineUniversity of Missouri-Kansas CityKansas CityMissouriMOUSA Institute of Translational Medicine Nanchang UniversityNanchangJiangxiP.R.China Department of Cell Biology and Anatomy University of South Carolina School of MedicineColumbiaSCUSA Division of Molecular Biology and Biochemistry School of Biological SciencesUniversity of Missouri-Kansas CityKansas CityMissouriMOUSA Division of Hematology and Medical Oncology Weill Cornell Medical CollegeNew YorkNYUSA Division of Pharmacology and Toxicology School of PharmacyUniversity of Missouri-Kansas CityKansas CityMissouriMOUSA

Although systemic inflammatory responses attributable to infection may lead to significant lung injury,the precise molecular mechanisms leading to lung damage are poorly understood and therapeutic options remain limited.Here,we show that myeloid monocyte chemotactic protein-inducible protein 1(MCPIP1)plays a central role in protecting against LPS-induced inflammation and lung injury.Myeloid-specific MCPIP1 knockout mice developed spontaneous inflammatory syndromes,but at a late age compared to global MCPIP1 knockout mice.Moreover,mice with a myeloid-specific deletion of MCPIP1 were extremely sensitive to LPS-induced lung injury due to overproduction of proinflammatory cytokines and chemokines.We identified C/EBPβand C/EBPδ,two critical transcriptional factors that drive cytokine production and lung injury,as targets of MCPIP1 RNase.LPS administration caused MCPIP1 protein degradation in the lungs.Pharmacological inhibition of MALT1,a paracaspase that cleaves MCPIP1,by MI-2 selectively increased the MCPIP1 protein levels in macrophages and in the lungs.Meanwhile,administration of MI-2 protected mice from LPS-induced inflammation,lung injury and death.Collectively,these results indicate that myeloid MCPIP1 is central in controlling LPS-induced inflammation and lung injury.Pharmacological inhibition of MALT1 protease activity may be a good strategy to treat inflammatory diseases by enhancing MCPIP1 expression in myeloid cells.

关键词： inflammation myeloid lung

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