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Absorbable 3D-printed pancreaticojejunostomy device with a dual-layer drug coating for the prevention of postoperative local recurrence of pancreatic cancer

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Journal of Materials Science & Technology 2023年第23期154卷 178-188页

作者： Maoen Pan Tingting Huang Zeya Xu Wei Luo Yuanyuan Yang tianhong Teng Heguang Huang Department of General Surgery Fujian Medical University Union Hospital Fuzhou China Key Laboratory of Optoelectronic Materials Chemical and Physics Chinese Academy of Sciences Fujian Institute of Research on the Structure of Matter Fuzhou China

The high local recurrence rate of pancreatic cancer after surgery is one of the important risk factors affecting patient *** traditionally used silicone tube stent is not only complicated to operate but also lacks antitumor *** purpose of this study was to develop a dual-layer drug-coated pancreaticojejunostomy *** coating consisted of two layers,an outer basic fibroblast growth factor(bFGF)coating layer and an inner nanoparticle albumin-bound paclitaxel(nab-PTX)coating layer with chitosan as the drug-carrying *** to the diffusion barrier from the outer coating,the release of nab-PTX from the inner layer was delayed and slowed *** studied the degradation rates,mechanical properties,surface morphologies,drug release kinetics,promoting the growth of fibroblasts and antitumor properties of the coated *** was found that 100 ng of bFGF and 50µg of nab-PTX were suitable drug concentrations that can effectively promote the growth of fibroblasts and inhibit pancreatic cancer *** results also confirmed that the dual-layer drug-coated pancreaticojejunostomy device showed good antitumor activity both in vitro and in vivo without obvious systemic *** addition,the device has a suitable degradation *** conclusion,this biodegradable dual-layer drug-coated pancreaticojejunostomy device can potentially inhibit the local recurrence of pancreatic cancer after surgery and promote the healing of pancreaticointestinal *** device has great potential to treat pancreatic cancer in the future.

关键词： Pancreaticojejunostomy device 3D printing Drug coating Anti-tumor effect Pancreatic cancer

A rapid and sensitive determination of paclitaxel in rat plasma by UPLC-MS/MS method: Application to a pharmacokinetic study

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Asian Journal of Pharmaceutical Sciences 2013年第3期8卷 199-205页

作者： He Lian Jin Sun tianhong zh.ng Department of Biopharmaceutics School of PharmacyShenyang Pharmaceutical UniversityMailbox 59#No.103Wenhua RoadShenyang 110016China

A rapid and sensitive method for quantitative determination of paclitaxel in rat plasmawas developed and validated by using ultra-performance liquid chromatography-tandemmass spectrometry (UPLC-MS/MS). Docetaxel was used as an internal standard anddiethyl ether was the liquideliquid extraction agent. Multiple reaction monitoring (MRM)mode via positive electrospray ionization (ESI) was applied to detect paclitaxel and IS at thetransitions m/z 854 / 286 and m/z 808.48 / 527.3, respectively. This method covered alinearity range from 5 to 5000 ng/ml, with the total run time of 3.0 min. In summary, a highthroughout UPLC-MS/MS method was successfully developed to measure paclitaxel in ratplasma and was applied to pharmacokinetic study after intravenous administration ofpaclitaxel.

关键词： Paclitaxel UPLC-MS/MS method Liquideliquid extraction Pharmacokinetic study

Pharmacokinetic performance of the nitrendipine intravenous submicron emulsion in rats

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Asian Journal of Pharmaceutical Sciences 2014年第6期9卷 330-335页

作者： Jibin Guan Nana zhao Yinglei zhai Chunxia Chu Hongming Chen tianhong zh.ng Shenyang Pharmaceutical University School of PharmacyNo.103Wenhua RoadShenyang 110016China

To compare pharmacokinetic behaviors of nitrendipine submicron emulsion with nitrendipine solution following intravenous administration in *** plasma concentrations were analyzed by ultra-performance liquid chromatography coupled with tandem mass spectrometry detection(UPLC-MS/MS)through a new validated *** pharmacokinetic parameters of the nitrendipine submicron emulsion and nitrendipine solution were as follows:AUC_(0-t) 900.76±186.59 versus 687.08±66.24 ng h/ml,C_(max) 854.54±159.48 versus 610.59±235.99 ng/ml,t_(1/2)2.37±1.99 versus 2.80±2.69 *** relative bioavailability of nitrendipine submicron emulsion to nitrendipine solution was 131.4±11.3%.The developed methods could meet the requirements of *** to the solution injection,intravenous submicron emulsion presents higher systematic exposure which can help to improve the therapeutic efficacy.

关键词： Nitrendipine Submicron emulsion Bioavailability Method validation

Relationships between selfreflectiveness and clinical symptoms in individuals during pre-morbid and early clinical stages of psychosis

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General Psychiatry 2022年第3期35卷 163-172页

作者： Lihua Xu Huiru Cui Yanyan Wei zhenying Qian Xiaochen Tang Yegang Hu Yingchan Wang Hao Hu Qian Guo Yingying Tang tianhong zh.ng Jijun Wang Shanghai Intelligent Psychological Evaluation and Intervention Engineering Technology Research Center Shanghai Key Laboratory of Psychotic DisordersShanghai Mental Health CenterShanghai Jiao Tong University School of MedicineShanghaiChina Department of Psychiatry Shanghai Mental Health CenterShanghai Jiao Tong University School of MedicineShanghaiChina Department of Early Psychotic Disorders Shanghai Mental Health CenterShanghai Jiao Tong University School of MedicineShanghaiChina Center for Excellence in Brain Science and Intelligence Technology(CEBSIT) Chinese Academy of ScienceShanghaiChina Brain Science and Technology Research Center Shanghai Jiao Tong UniversityShanghaiChina

Background Self-reflectiveness,one dimension of cognitive insight,plays a protective role in an individual’s mental *** high and low levels of self-reflectiveness have been reported in patients with schizophrenia and individuals at clinical high risk for the *** This study aimed to explore the relationship patterns between self-reflectiveness and clinical symptoms in individuals during the pre-morbid and early clinical stages of *** A total of 181 subjects,including individuals with attenuated positive symptoms(APS,n=122)and patients with first-episode psychosis(FEP,n=59),completed the Beck Cognitive Insight Scale and were evaluated using the Schedule of Assessment of Insight and Positive and Negative Syndrome *** subjects were classified into three groups according to their level of selfreflectiveness:low level(LSR,n=59),medium level(MSR,n=67)and high level(HSR,n=55).Both linear and nonlinear relationships between self-reflectiveness and clinical symptoms were *** More individuals with APS were classified into the MSR group,while more patients with FEP were classified into the LSR *** LSR group demonstrated less awareness of illness than the MSR and HSR groups,more stereotyped thinking and poorer impulse control but less anxiety than the MSR group,and lower levels of blunted affect and guilt feelings than the HSR *** MSR group demonstrated lower stereotyped thinking than the HSR *** to the LSR group,the MSR group had increased selfreflectiveness,improved awareness of illness,decreased stereotyped thinking,and better impulse control,but increased feelings of *** HSR group showed increased stereotyped thinking when compared to the MSR group,but the other variables did not change significantly between these two ***,self-reflectiveness demonstrated an approximately inverse S-shaped relationship with the awareness of illness,a U-shaped relationship with stereotyped thinking and poor

关键词： clinical symptoms morbid

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桃SSR反应体系的优化

中国农业大学学报 2005年第6期10卷 57-61页

作者：李银霞李天红中国农业大学农学与生物技术学院北京100094

以‘新大久保’桃为试材,利用正交设计直观分析法和2因素完全随机试验优化了桃SSR技术中PCR反应体系.试验结果表明,适宜桃遗传分析的SSR技术体系为:在25 μL反应体系中Taq DNA聚合酶和DNA最适用量分别为1 U和50～100 ng;Mg2+ 、dNTP和... 详细信息

以‘新大久保’桃为试材,利用正交设计直观分析法和2因素完全随机试验优化了桃SSR技术中PCR反应体系.试验结果表明,适宜桃遗传分析的SSR技术体系为:在25 μL反应体系中Taq DNA聚合酶和DNA最适用量分别为1 U和50～100 ng;Mg2+ 、dNTP和引物最适终浓度分别为1.5～2.0 mmol/L、0.20～0.28 mmol/L和0.2～0.6 μmol/L.利用30个桃品种验证此反应体系,变性聚丙烯酰胺凝胶电泳检测结果显示,扩增产物在100～300 bp,多态性高,且反应体系的稳定性和可重复性好.

关键词：桃 SSR 体系优化正交直观分析法完全随机试验

融合蛋白GSH-bFGF的构建、表达及体外活性检测

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暨南大学学报（自然科学与医学版） 2015年第2期36卷 108-113页

作者：陈霖周天鸿邹奕暨南大学生命科学技术学院生物工程系广东广州510632

目的:设计构建和表达新型融合蛋白谷胱甘肽-碱性成纤维细胞生长因子(Glutathione-basic fibroblast growth factor,GSH-b FGF),并检测其体外抗氧化、抗衰老能力.方法:采用RT-PCR,酶切,连接,转化等分子克隆技术构建新型融合蛋白GSH-b FGF... 详细信息

目的:设计构建和表达新型融合蛋白谷胱甘肽-碱性成纤维细胞生长因子(Glutathione-basic fibroblast growth factor,GSH-b FGF),并检测其体外抗氧化、抗衰老能力.方法:采用RT-PCR,酶切,连接,转化等分子克隆技术构建新型融合蛋白GSH-b FGF,并通过镍柱亲和层析、阳离子交换层析纯化该目的蛋白.通过CCK-8试剂盒检测GSH-b FGF对鼠源成纤维细胞NIH-3T3的促增殖活性.同时,经总抗氧化检测试剂盒检测GSH-b FGF的抗氧化能力.结果:克隆表达并纯化获得融合蛋白GSH-b ***-8促增殖结果显示质量浓度为20 ng/m L的GSH-b FGF可显著促进鼠源成纤维细胞NIH-3T3增殖.体外抗氧化结果显示GSH-b FGF有显著的抗氧化活性,纯化得到的GSHb FGF蛋白的GSH浓度约为(0.06±0.02)μmol/L,抗氧化能力为(0.56±0.23)mmol/g.结论:新型融合蛋白GSHb FGF有显著的体外促增殖能力及抗氧化活性.

关键词：谷胱甘肽-碱性成纤维生长因子促增殖能力抗氧化活性

超高效液相色谱法同时测定布洛芬注射液中布洛芬和精氨酸的含量

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色谱 2011年第10期29卷 1005-1009页

作者：刘静马鹤群朱萌王欢张天虹沈阳药科大学辽宁沈阳110016

建立了一种同时测定布洛芬注射液中布洛芬和精氨酸含量的超高效液相色谱方法。精氨酸与衍生化试剂2,4-二硝基氟苯(DNFB)反应后,与布洛芬同时在超高效液相色谱-二极管阵列检测器(UPLC-PDA)上检测。采用BEH C18色谱柱(50 mm×2.1 mm,1.7μ... 详细信息

建立了一种同时测定布洛芬注射液中布洛芬和精氨酸含量的超高效液相色谱方法。精氨酸与衍生化试剂2,4-二硝基氟苯(DNFB)反应后,与布洛芬同时在超高效液相色谱-二极管阵列检测器(UPLC-PDA)上检测。采用BEH C18色谱柱(50 mm×2.1 mm,1.7μm),以乙腈-0.05 mol/L磷酸二氢钾缓冲液(pH 2.5)为流动相进行梯度洗脱,流速为0.4 mL/min,柱温为30℃,检测波长分别为357 nm(精氨酸衍生物)和220 nm(布洛芬)。结果表明,布洛芬与精氨酸分别在2.0～100.5 mg/L和1.7～84.5 mg/L范围内呈良好的线性关系,相关系数(r)均为0.999 7;平均回收率分别为99.8%和99.6%,相对标准偏差(RSDs)分别为0.37%和0.25%;定量限(信噪比(S/N)=10)分别为0.1 ng和0.2 ng;检出限(S/N=3)分别为0.03 ng和0.05 ng。本方法快速、准确,重复性好,可较全面地评价布洛芬注射液的质量。

关键词：超高效液相色谱柱前衍生布洛芬精氨酸布洛芬注射液

25-羟维生素D诊断弥漫性大B细胞淋巴瘤患者的临床特征及预后关系

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临床血液学杂志 2022年第8期35卷 556-559页

作者：张荣任天红石家庄市第三医院检验科石家庄050001

目的探讨可利用生物25-羟维生素D[25(OH)D]水平与弥漫性大B细胞淋巴瘤(DLBCL)患者临床特征及预后的关系。方法收集2017年3月至2019年5月收治的诊断为DLBCL患者124例作为DLBCL组,另选择同时期进行健康体检且结果正常者39例作为对照组。检... 详细信息

目的探讨可利用生物25-羟维生素D[25(OH)D]水平与弥漫性大B细胞淋巴瘤(DLBCL)患者临床特征及预后的关系。方法收集2017年3月至2019年5月收治的诊断为DLBCL患者124例作为DLBCL组,另选择同时期进行健康体检且结果正常者39例作为对照组。检测2组25(OH)D水平,分析患者一般临床资料和相关实验室检测指标,分析25(OH)D水平与患者临床病理特征,Kaplan-Meier分析法分析25(OH)D水平与患者累积生存时间的关系,Cox比例风险回归模型分析患者预后的独立危险因素。结果DLBCL组患者25(OH)D水平明显低于对照组;25(OH)D水平与DLBCL患者年龄、肿瘤分期、乳酸脱氢酶、肝功能、靶器官受损和B症状有关;25(OH)D水平ng/mL的累积生存时间明显小于25(OH)D水平≥18.36 ng/mL的患者(P<0.05);Cox分析发现Ann Arbor分期、25(OH)D和年龄是DLBCL患者预后的独立危险因素。结论DLBCL患者体内25(OH)D水平明显低于对照组,25(OH)D水平与患者临床病理特征具有一定的相关性,并且是患者预后的独立危险因素,值得临床关注。

关键词： 25-羟维生素D 弥漫性大B细胞淋巴瘤临床特征预后

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考虑粘结滑移的新型斜柱转换结构静力分析

水利与建筑工程学报 2020年第4期18卷 99-104,110页

作者：张季阳范余辉朱勇超吴洪梅郑田洪刘博贵州大学空间结构研究中心贵州贵阳550025

新型斜柱转换结构改善了传统斜柱转换结构的抗震性能。为了研究新型斜柱转换结构在考虑粘结滑移时的静力力学特征,利用ABAQUS建立3组共6个有限元模型。采用三个不同的轴压比,每个轴压比两个模型,其中一个型钢与混凝土之间建立非线性弹... 详细信息

新型斜柱转换结构改善了传统斜柱转换结构的抗震性能。为了研究新型斜柱转换结构在考虑粘结滑移时的静力力学特征,利用ABAQUS建立3组共6个有限元模型。采用三个不同的轴压比,每个轴压比两个模型,其中一个型钢与混凝土之间建立非线性弹簧模拟粘结滑移作用,另一个完全嵌固忽略粘结滑移作用。对比分析表明:在静力加载作用下,考虑型钢混凝土粘结滑移的模型相对忽略粘结滑移作用的模型,其荷载位移曲线有较明显的下降段,更为符合实际情况,可以在实际工程中应用此方法进行模拟。

关键词：新型斜柱转换结构粘结滑移数值模拟轴压比