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RebL1 is required for macronuclear structure stability and gametogenesis in Tetrahymena thermophila

Marine Life Science & Technology 2024年第2期6卷 183-197页

作者： Huijuan hao Yinjie Lian Chenhui ren Sitong Yang Min Zhao Tao Bo Jing Xu Wei Wang Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education Institute of BiotechnologyShanxi UniversityTaiyuan 030006China School of Life Science Shanxi UniversityTaiyuan 030006China Shanxi Key Laboratory of Biotechnology Taiyuan 030006China

Histone modification and nucleosome assembly play important roles in chromatin-related *** chaperones form different complexes and coordinate histone transportation and *** histone chaperone complexes have been identified in different *** ciliate protozoa(ciliates)have various chromatin structures and different nuclear ***,histone chaperone components and functions of different subunits remain unclear in ***-rahymema thermophila contains a transcriptionally active macronucleus(MAC)and a transcriptionally inactive micronu-cleus(MIC)which exhibit multiple replication and various chromatin remodeling progresses during vegetative growth and sexual developmental ***,we found histone chaperone RebL1 not only localized evenly in the transcriptionally active MAC but also dynamically changed in the MIC during vegetative growth and sexual developmental ***1 knockdown inhibited cellular *** macronuclear morphology became bigger in growing *** abnormal macronuclear structure also occurred in the starvation ***,micronuclear meiosis was disturbed during sexual development,leading to a failure to generate new gametic ***1 potentially interacted with various factors involved in histone-modifying complexes and chromatin remodeling complexes in different developmental ***1 knockdown affected expression levels of the genes involved in chromatin organization and *** together,RebL1 plays a vital role in maintaining macronuclear structure stability and gametogenesis in ***.

关键词： Gametogenesis Histone chaperone Macronuclear structure RebL1 Tetrahymena thermophilaIntroduction

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Transfer of disulfide bond formation modules via yeast artificial chromosomes promotes the expression of heterologous proteins in Kluyveromyces marxianus

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mLife 2024年第1期3卷 129-142页

作者： Pingping Wu Wenjuan Mo tian tian Kunfeng Song Yilin Lyu Haiyan ren Jungang Zhou Yao Yu Hong Lu State Key Laboratory of Genetic Engineering School of Life SciencesFudan UniversityShanghaiChina Shanghai Engineering Research Center of Industrial Microorganisms ShanghaiChina

Kluyveromyces marxianus is a food-safe yeast with great potential for producing heterologous *** the yield in *** remains a challenge and incorporating large-scale functional modules poses a technical obstacle in *** address these issues,linear and circular yeast artificial chromosomes of ***(KmYACs)were constructed and loaded with disulfide bond formation modules from Pichia pastoris or *** modules contained up to seven genes with a maximum size of 15 *** carried telomeres either from *** or *** were transferred successfully into *** and stably propagated without affecting the normal growth of the host,regardless of the type of telomeres and configurations of *** increased the overall expression levels of disulfide bond formation genes and significantly enhanced the yield of various heterologous *** high-density fermentation,the use of KmYACs resulted in a glucoamylase yield of 16.8 g/l,the highest reported level to date in *** and metabolomic analysis of cells containing KmYACs suggested increased flavin adenine dinucleotide biosynthesis,enhanced flux entering the tricarboxylic acid cycle,and a preferred demand for lysine and arginine as features of cells overexpressing heterologous ***,supplementing lysine or arginine further improved the ***,KmYAC provides a powerful platform for manipulating large modules with enormous potential for industrial applications and fundamental *** the disulfide bond formation module via YACs proves to be an efficient strategy for improving the yield of heterologous proteins,and this strategy may be applied to optimize other microbial cell factories.

关键词： disulfide bond formation expression of heterologous proteins Kluyveromyces marxianus telomere yeast artificial chromosome

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Dual-curvilinear beam enabled tunable manipulation of high-and low-refractive-index particles

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Photonics Research 2024年第7期12卷 1427-1436页

作者： ZHENG YUAN CHENCHEN ZHANG YUAN GAO WENXIANG YAN XIAN LONG ZHI-CHENG ren XI-LIN WANG JIANPING DING HUI-tian WANG National Laboratory of Solid State Microstructures and School of Physics Nanjing UniversityNanjing 210093China Collaborative Innovation Center of Advanced Microstructures Nanjing 210093China Collaborative Innovation Center of Solid-State Lighting and Energy-Saving Electronics Nanjing 210093China

We present an innovative approach for the simultaneous agile manipulation of high-refractive-index(HRI) and low-refractive-index(LRI) particles. Our method involves introducing a dual-curvilinear optical vortex beam(DC-OVB) generated by superimposing a pair of curved beams: HRI and LRI particles are controlled by the bright curve and the dark channel between the two curves, respectively. The proposed DC-OVB provides customizable motion paths and velocities for both LRI and HRI particles. Each curve of the DC-OVB can support a distinct orbital flow density(OFD), enabling the application of torques to HRI and LRI particles, guiding them to orbit along specified trajectories and prompting them to execute various curvilinear motions simultaneously,including curvilinear movement, revolution, and rotation.

关键词： tunable refractive beam

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Introduction of rare-earth element Sc in alloy design to modify wear features of dual-phase high-entropy alloy

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Rare Metals 2024年第2期43卷 817-828页

作者： hao ren Rui-Run Chen Xue-Feng Gao Tong Liu Gang Qin Yu-Lung Chiu Shi-Ping Wu Jing-Jie Guo Guo National Key Laboratory for Precision Hot Processing of Metals School of Materials Science and EngineeringHarbin Institute of TechnologyHarbin150001China School of Metallurgy and Materials University of BirminghamBirminghamB152TTUK

Tailoring the alloy composition,which induces the hard secondary phase to increase hardness and strength to improve the wear features,is a feasible approach for developing wear-resistant metal ***,a group of(AlCoCrFeNi)_(100–x)Sc_(x)(x=0–2.0,at%)high-entropy alloys(HEAs)are designed and the phase compositions and wear behaviors are ***-doped HEA series contain the primary body-centered cubic(BCC)and eutectic phases,in which the eutectic phase is composed of the alternately grown BCC and Laves *** addition promotes the diffusion of Ni atoms from BCC phase to form the Sc-rich Laves phase at the grain *** hardness increases due to solid solution strengthening and second phase *** the second phase strengthening plays a more significant role relative to solid solution *** phase and the oxides caused by wear heating prevent the direct contact between friction pair and HEAs,thus inducing a decreased wear rate from 6.82×10^(−5) to 3.47×10^(−5)m^(3)·N^(−1)·m^(−1).Moreover,the wear mechanism changes from adhesive wear,abrasive wear and oxidative wear to abrasive wear and oxidative wear.

关键词： High entropy alloy(HEA) Laves phase Hardness Wear mechanism

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Analysis of vascular thrombus and clinicopathological factors in prognosis of gastric cancer:A retrospective cohort study

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World Journal of Gastrointestinal Oncology 2024年第8期16卷 3436-3444页

作者： Guo-Yue Chen Ping ren Zhen Gao hao-Ming Yang Yan Jiao Department of Radiology Jinan Central HospitalJinan 250013Shandong ProvinceChina Department of Gastrointestinal Surgery Jiangsu Provincial People's HospitalNanjing 210029Jiangsu ProvinceChina

BACKGROUND Gastric cancer(GC)is one of the most common malignant tumors in the world,and its prognosis is closely related to many *** recent years,the incidence of vascular thrombosis in patients with GC has gradually attracted increasing attention,and studies have shown that it may have a significant impact on the survival rate and prognosis of ***,the specific mechanism underlying the association between vascular thrombosis and the prognosis of patients with GC remains *** To analyze the relationships between vascular cancer support and other clinicopathological factors and their influence on the prognosis of patients with *** This study retrospectively analyzed the clinicopathological data of 621 patients with GC and divided them into a positive group and a negative group according to the presence or absence of a vascular *** difference in the 5-year cumulative survival rate between the two groups was compared,and the relationships between vascular cancer thrombus and other clinicopathological factors and their influence on the prognosis of patients with GC were *** Among 621 patients with GC,the incidence of vascular thrombi was 31.7%(197 patients).Binary logistic regression analysis revealed that the degree of tumor differentiation,depth of invasion,and extent of lymph node metastasis were independent influencing factors for the occurrence of vascular thrombi in GC patients(Prend of the χ^(2) test showed that the degree of differentiation,depth of invasion,and extent of lymph node metastasis were linearly correlated with the percentage of vascular thrombi in GC patients(P<0.01),and the correlation between lymph node metastasis and vascular thrombi was more significant(r=0.387).Univariate analysis revealed that the 5-year cumulative survival rate of the positive group was significantly lower than that of the negative group(46.7%vs 73.3%,P<0.01).Multivariate analysis revealed that age,tumor diam

关键词： Vascular cancer thrombus Gastric cancer Survival prognosis TNM staging Retrospective study

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Experimental Study on Vortex-Induced Vibration of Rough Risers with Coupling Interference Effect Under Side-by-Side Arrangement

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China Ocean Engineering 2024年第1期38卷 18-28页

作者： HU Ze-bo LIU Zhen LI Peng GUO Hai-yan WANG Shu-bing ren Xiao-hui HOU hao WANG Ye-shuo College of Civil Engineering and Architecture Shandong University of Science and TechnologyQingdao 266590China Qingdao Key Laboratory of Marine Civil Engineering Materials and Structures Qingdao 266590China College of Engineering Ocean University of ChinaQingdao 266100China CHEC Dredging Co. LtdShanghai 200136China

A vortex-induced vibration(VIV)experiment of rough risers with coupling interference effect under a side-by-side arrangement was carried out in a wave-current combined *** roughness of the riser was characterized by arranging different specifications of surface attachments on the surface of the *** risers with three different roughnesses were arranged side by side with smooth risers to explore the VIV response of the riser under the combined action of roughness and interference effect,and to reveal the coupling mechanism between roughness and interference *** experimental results show that,compared with that of a smooth riser,the VIV of a rough riser under the coupling interference effect has a wider"lock-in"region,and the displacement decreases more significantly at a high reduced velocity,which is more likely to excite higher-order modes and frequency *** addition,the displacement response and frequency response of the smooth riser are not significantly affected by wake interference from the rough riser,which is caused by the decrease of the wake region due to the delay of the boundary layer separation point of the rough riser.

关键词： marine riser vortex-induced vibration side-by-side arrangement roughness interference effect

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A Hf-doped dual-phase high-entropy alloy: phase evolution and wear features

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Rare Metals 2024年第1期43卷 324-333页

作者： hao ren Rui-Run Chen Xue-Feng Gao Tong Liu Gang Qin Yu-Lung Chiu Shi-Ping Wu Jing-Jie Guo National Key Laboratory for Precision Hot Processing of Metals School of Materials Science and EngineeringHarbin Institute of TechnologyHarbin 150001China School of Metallurgy and Materials University of BirminghamEdgbastonBirmingham B152TTUK

Initially defined high entropy alloys(HEAs)usually exhibit a single-phase solid-solution ***,two and/or more types of phases in HE As possibly induce the desired microstructure features,which contribute to improving the wear properties of HE ***,we prepare a series of(AlCoCrFeNi)_(100-x)Hf_(x)(x=0,2,4and 6;at%) HEAs and concern their phase compositions,micro structures and wear *** leads to the formation of(Ni,Co)_(2)Hf-type Laves phase and tailors the microstructure from a body-centered cubic(BCC) singlephase structure to a hypoeutectic *** increased hardness from~HV 512.3 to~HV 734.1 is due to solid-solution strengthening,grain refinement strengthening and precipitated phase *** a few oxides(Al_(2)O_(3)+Cr_(2)O_(3)) caused by the wear heating contribute to an 85.5% decrease in wear rate of the HEA system from6.71×10^(-5) to 0.97×10^(-5) m^(3)·N^(-1)·m^(-1).In addition,Hf addition changes the wear mechanism from abrasive wear,mild oxidative wear and adhesive wear to oxidative wear and adhesive wear.

关键词： High entropy alloy Laves phase Hardness Wear mechanism

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Accelerating the Control and Elimination of Major Parasitic Diseases in China—On World NTD Day 2024

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China CDC weekly 2024年第6期6卷 95-99页

作者： Yuwan hao tian tian Zelin Zhu Yijun Chen Jing Xu Shuai Han Menbao Qian Yi Zhang Shizhu Li Qiang Wang National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Institute of Parasitic DiseasesChinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research)Key Laboratory on Parasite and Vector BiologyMinistry of HealthWHO Centre for Tropical Diseases National Center for International Research on Tropical Diseases Ministry of Science and TechnologyShanghaiChinaSchool of Global HealthChinese Center for Tropical Diseases Research-Shanghai Jiao Tong University School of MedicineShanghaiChina

Neglected tropical diseases(NTDs)are a group of diseases associated with poverty that affect over 1 billion people worldwide(1).On May 31,2021,the World Health Assembly(WHA)officially recognized January 30 as World Neglected Tropical Diseases Day through decision WHA74(18).This designation aims to raise awareness about the severe impact of NTDs on disadvantaged populations and to mobilize support for control,elimination,and eradication efforts.

关键词： Diseases diseases disadvantage

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Solar-driven photothermal catalytic CO_(2) conversion:a review

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Rare Metals 2024年第7期43卷 2913-2939页

作者： Bachirou Guene Lougou Bo-Xi Geng Ru-Ming Pan Wei Wang tian-tian Yan Fang-Hua Li hao Zhang Oraléou SanguéDjandja Yong Shuai Meisam Tabatabaei Daniel Sabi Takou School of Energy Science and Engineering Harbin Institute of TechnologyHarbin 150001China MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage Harbin Institute of TechnologyHarbin 150001China State Key Laboratory of Urban Water Resource and Environment Harbin Institute of TechnologyHarbin 150090China School of Electrical Engineering and Automation Harbin Institute of TechnologyHarbin 150001China Guangdong Provincial Key Laboratory of Distributed Energy Systems Engineering Research Center of None-Food Biomass Efficient Pyrolysis and Utilization Technology of Guangdong Higher Education InstitutesDongguan University of TechnologyDongguan 523808China Higher Institution Centre of Excellence(HICoE) Institute of Tropical Aquaculture and Fisheries(AKUATROP)Universiti Malaysia Terengganu21030 Kuala NerusMalaysia Department of Biomaterials Saveetha Dental CollegeSaveetha Institute of Medical and Technical SciencesChennai600077India école Polytechnique d'Abomey-Calavi University of Abomey-Calavi2009CotonouBenin

It is highly desirable to seek green and sustainable technologies,such as employing photo thermal effects to drive energy catalysis processes to address the high energy demand and associated environmental impacts induced by the current *** photothermocatalysis process is an emerging research area with great potential in efficiently converting solar energy through various catalytic ***,achieving simultaneously high conversion efficiency,cyclability,and durability is still a daunting ***,tremendous work is still needed to enhance solar photo thermal catalytic conversion and promote its large-scale *** review developed the principles of coupling solar photon and thermal fields underlying the photothermal effect,exploration of efficient nanocatalysts,development of optofluidic reactor model,and photo thermal synergistic-driven CO_(2) reduction *** ultimate goal was to provide an effective approach that can effectively convert solar energy into photocarriers/hot-electrons and heat,and importantly,can couple them to regulate catalysis reaction pathways toward the production of value-added fuel and chemical energy.

关键词： Photochemistry Photothermal CO_(2)catalytic conversion Nanomaterial Solar fuels and chemicals

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Modulation of host N6-methyladenosine modification by gut microbiota in colorectal cancer

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World Journal of Gastroenterology 2024年第38期30卷 4175-4193页

作者： tian-Qi Jiang hao Wang Wang-XinJun Cheng Chuan Xie Department of Gastroenterology Jiangxi Provincial Key Laboratory of Digestive DiseasesJiangxi Clinical Research Center for Department of GastroenterologyDigestive Disease HospitalThe First Affiliated HospitalJiangxi Medical CollegeNanchang UniversityNanchang 330006Jiangxi ProvinceChina The First Clinical Medical College Nanchang UniversityNanchang 330006Jiangxi ProvinceChina Queen Mary College Nanchang UniversityNanchang 330006Jiangxi ProvinceChina

As a research hotspot in the field of molecular biology,N6-methyladenosine(m6A)modification has made progress in the treatment of colorectal cancer(CRC),leukemia and other *** studies have demonstrated that the tumour microenvironment(TME)regulates the level of m6A modification in the host and activates a series of complex epigenetic signalling pathways through interactions with CRC cells,thus affecting the progression and prognosis of ***,with the diversity in the composition of TME factors,this action is reci-procal and ***,some studies have experimentally revealed that the intestinal flora can alter CRC cell proliferation by directly acting on m6A and thereby altering CRC cell *** review summarizes the data,supporting the idea that the intestinal flora can influence host m6A levels through pathways such as methyl donor metabolism and thus affect the progression of *** also review the role of m6A modification in the diagnosis,treatment,and prognostic assessment of CRC and discuss the current status,limitations,and potential clinical value of m6A modification in this *** propose that additional in-depth research on m6A alterations in CRC patients and their TME-related targeted therapeutic issues will lead to better therapeutic outcomes for CRC patients.

关键词： Colorectal cancer N6-methyladenosine Tumour microenvironment Gut microbiota Clinical application

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