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Diversity and dynamics of microbial communities in brown planthopper at different developmental stages revealed by high-throughput amplicon sequencing

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Insect Science 2020年第5期27卷 883-894页

作者： Zheng-Liang wang tian-Zhao wang Hang-Feng Zhu Hai-Bo Pan Xiao-ping Yu Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine College of Life SciencesChina Jiliang UniversityHangzhouChina

The microbiome associated with brown planthopper(BPH)plays an important role in mediating host health and *** of the microbial community and its structure is prerequisite for understanding the intricate symbiotic relationships between microbes and host ***,we investigated the bacterial and fungal communities of BPH at different developmental stages using high-throughput amplicon *** results revealed that both the bacterial and fungal communities were diverse and dynamic during BPH *** bacterial communities were generally richer than fungi in each developmental *** 97%similarly,19 phyla and 278 genera of bacteria were an-notated,while five fungal phyla comprising 80 genera were *** highest species richness for the bacterial communities was detected in the nymphal *** taxonomic diversity of the fungal communities in female adults was generally at a relatively higher level when compared to other developmental *** most dominant phylum of bacteria and fungi at each developmental stage all belonged to Proteobacteria and Ascomycota,re-spectively.A significantly lower abundance of bacterial genus Acinetobacter was recorded in the egg stage when compared to other developmental stages,while the dominant fun-gal genus Wallemia was more abundant in the nymph and adult stages than in the egg ***,the microbial composition differed between male and female adults,suggesting that the microbial communties In BPH were ***,our study enriches our knowledge on the microbial communities associated with BPH and will provide clues to develop potential biocontrol techniques against this rice pest.

关键词： amplicon sequencing brown planthopper dynamics microbial diversity microbiome

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Characteristics of timberline and treeline altitudinal distribution in *** and their geographical interpretation

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Journal of Mountain Science 2022年第10期19卷 2846-2860页

作者： MU Hao-xiang HAN Fang ZHANG Bai-ping LIANG tian wang Zhi-yong wang Zhe School of Civil and Architectural Engineering Shandong University of TechnologyZibo 255000China State Key Lab for Resources and Environment Information System Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101China Center for Yellow River Ecosystem Products Shandong UniversityQingdao 266237China

Different types of vegetation patches are alternately and randomly distributed in a timberline ecotone where the upper limit is the treeline and the lower limit is the ***,most studies on timberline/treeline altitudinal distributions have simplified timberline or treeline as continuous curves and disregarded the fuzziness of timberline/treeline and the randomness of different vegetation patch distributions in a timberline *** study the altitudinal distribution characteristics of timberline and treeline from the perspective of uncertainty theory,we constructed the timberline and treeline elevation cloud models in *** in east ***,we established multiple linear regression models by using nine influencing factors,namely,aspect,slope,topographic relief,dryness index,average temperature in January and July,latitude,summit syndrome(represented by the vertical distance from the peak),and snow effect(represented by the nearest distance from the snow)as independent variables,and the elevations of timberline/treeline as dependent *** we compared the contributions of the nine factors in timberline,treeline,and the core and peripheral areas of timberline and *** results show that 1)the timberline/treeline elevation cloud model can represent the overall characteristics(especially the uncertainty)of the altitudinal distributions of the timberline/treeline *** uncertainty of treeline’s altitudinal distribution is higher than that of timberline(entropy and hyper entropy:207.59 m and 70.36 m for treeline elevation cloud;entropy and hyper entropy:191.17 m and 50.13 m for timberline elevation cloud).2)The influence of climate and topography on timberline and treeline are *** average temperature in July has a significant negative correlation with the timberline/treeline elevation in ***,which is the most critical factor that affects timberline and treeline elevation,explaining the altitudinal

关键词： Timberline Treeline Geographical interpretation Uncertainty Influencing factors Mt.Namjagbarwa

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Preparation and Properties of High Hardness and Oxidation Resisting Coating Using Electric Arc Spray

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材料热处理学报 2004年第05B期25卷 984-987页

作者： LIZhuo-xin CUILi wangJiang-ping TANGChun-tian BeijingUniversityofTechnology Beijing100022

A coating with high hardness, wear and oxidation resistance was prepared by electric arc spray. The hardness, bonding strength, abrasive wear and values of porosity and oxidation resistance of the coating were investigated. The microstructures and function of Cr3C2 of the coating were analyzed. The results showed surface Rockness Hardness HR30 reached 72.5 and average bond strength reached 49.1Mpa. Also porosity value was less than 2%. In addition, it was found from the comparison between the coating and 45CT coating that, oxidation resistance of the coating was less than that of 45CT, but the abrasive wear of the coating was obvious better than that of 45CT.

关键词：硬度磨损性能高温氧化电弧喷镀

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Resource reduction for simultaneous generation of two types of continuous variable nonclassical states

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Frontiers of physics 2021年第2期16卷 3-8页

作者： Long tian Shao-ping Shi Yu-Hang tian Ya-Jun wang Yao-Hui Zheng Kun-Chi Peng State Key Laboratory of Quantum Optics and Quantum Optics Devices Institute of Opto-ElectronicsShanxi UniversityTaiyuan 030006China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China

We demonstrate experimentally the simultaneous generation and detection of two types of continuous variable nonclassical states from one type-0 phase-matching optical parametric armplification(OPA)and subsequent two ring filter cavities(RFCs).The output field of the OPA includes the baseband wo and sideband modes ω0±nωf subjects to the cavity resonance condition,which are separated by two cascaded *** first RFC resonates with half the pump wavelength wo and the transmitted baseband component is a squeezed *** relcted fields of the first RFC,including the sideband modes ω0±wf,are separated by the second RFC,construct Einstein Podolsky-Rosen entangled *** freedoms,including the filter cavities for sideband separation and relative phases for the measure-ments of these sidebands,are actively *** noise variance of squeezed states is 10.2 dB below the shot noise limit(SNL),the correlation variances of both quadrature amplitude-sum and quadrature phase diference for the entanglement state are 10.0 dB below the corresponding SNL.

关键词： squeezed states entanglement states continuous variable non-classical states

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多层螺旋CT对肺结核合并肺癌患者的诊断价值

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现代生物医学进展 2017年第6期17卷 1106-1109页

作者：石德峰鲍天萍刘冲王淑梅李敏保定市第一中心医院影像科河北保定071000 保定市第一中心医院超声室河北保定071000 河北医科大学第四医院河北石家庄050031

目的:研究多层螺旋CT对肺结核合并肺癌的鉴别诊断价值。方法:选择2013年3月至2015年9月在我院确诊的肺结核合并肺癌患者32例和单纯肺结核患者39例应用多层螺旋CT扫描患者肺部病变情况。结果:肺结核合并肺癌组:陈旧性肺结核28例、活动性... 详细信息

目的:研究多层螺旋CT对肺结核合并肺癌的鉴别诊断价值。方法:选择2013年3月至2015年9月在我院确诊的肺结核合并肺癌患者32例和单纯肺结核患者39例应用多层螺旋CT扫描患者肺部病变情况。结果:肺结核合并肺癌组:陈旧性肺结核28例、活动性肺结核4例;病灶位置经典部位29例、非经典部位3例,合并鳞癌11例、腺癌13例、小细胞癌5例、未分化癌3例;10例结核病灶与肺癌病灶不同侧、13例结核病灶与肺癌病灶同侧不同叶、9例结核病灶于肺癌病灶同侧同叶。单纯性肺结核组胨旧性肺结核36例、活动性肺结核3例;病灶位置经典部位34例(上叶尖段11例、后段9例、下叶背段14例)、非经典部位5例。肺结核合并肺癌组患者分叶征、毛刺征、胸膜凹陷征、阻塞性肺炎及肺不张以及棘状突起比例高于单纯肺结核组,而空泡影比例低于单纯肺结核组,差异具有统计学意义(P0.05)。结论:多层螺旋CT对肺结核合并肺癌具有较高的临床鉴别诊断价值。

关键词：多层螺旋CT 肺结核肺癌诊断价值

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Tau-Induced Ca^(2+)/Calmodulin-Dependent Protein Kinase-IV Activation Aggravates Nuclear Tau Hyperphosphorylation

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Neuroscience Bulletin 2018年第2期34卷 261-269页

作者： Yu-ping Wei Jin-wang Ye Xiong wang Li-ping Zhu Qing-Hua Hu Qun wang Dan Ke Qing tian Jian-Zhi wang Pathophysiology Department Tongji Medical CollegeHuazhong University of Science and Technology Key Laboratory of Ministry of Education for Neurological Disorders and Hubei Provincial Key Laboratory for Neurological Disorders Tongji Medical College Huazhong University of Science and Technology

Hyperphosphorylated tau is the major protein component of neurofibrillary tangles in the brains of patients with Alzheimer’s disease(AD). However, the mechanism underlying tau hyperphosphorylation is not fully understood. Here, we demonstrated that exogenously expressed wild-type human tau40 was detectable in the phosphorylated form at multiple AD-associated sites in cytoplasmic and nuclear fractions from HEK293 *** these sites, tau phosphorylated at Thr205 and Ser214 was almost exclusively found in the nuclear fraction at the conditions used in the present study. With the intracellular tau accumulation, the Ca2+concentration was significantly increased in both cytoplasmic and nuclear fractions. Further studies using site-specific mutagenesis and pharmacological treatment demonstrated that phosphorylation of tau at Thr205 increased nuclear Ca2+concentration with a simultaneous increase in the phosphorylation of Ca2+/calmodulin-dependent protein kinase IV(Ca MKIV) at Ser196. On the other hand, phosphorylation of tau at Ser214 did not significantly change the nuclear Ca2+/Ca MKIV signaling. Finally, expressing calmodulin-binding protein-4 that disrupts formation ofthe Ca2+/calmodulin complex abolished the okadaic acidinduced tau hyperphosphorylation in the nuclear *** conclude that the intracellular accumulation of phosphorylated tau, as detected in the brains of AD patients, can trigger nuclear Ca2+/Ca MKIV signaling, which in turn aggravates tau hyperphosphorylation. Our findings provide new insights for tauopathies: hyperphosphorylation of intracellular tau and an increased Ca2+concentration may induce a self-perpetuating harmful loop to promote neurodegeneration.

关键词： Alzheimer’s disease Tau Phosphorylation Nuclear calcium signal CaMKIV

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Effect of temperature difference load of 32 m simply supported box beam bridge on track vertical irregularity

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Journal of Modern Transportation 2015年第4期23卷 262-271页

作者： Kaize Xie Jun Xing Li wang Chunxiang tian Rong Chen ping wang MOE Key Laboratory of High-Speed Railway Engineering Southwest Jiaotong University China Railway Eryuan Engineering Group Co.Ltd

In order to study the effect of temperature difference load （TDL） along the vertical direction of a simply supported beam bridge section on the vertical irregularity, a rail-bridge-piers calculation model was established. Taking 32 m simply supported box beam bridge which is widely used in the construction of pas- senger dedicated line in China as an example, influences of the temperature variation between the bottom and top of the bridge, temperature curve index, type of temperature gradient, and beam height on track vertical irregularity were analyzed with the model. The results show that TDL has more effects on long wave track irregularity than on short one, and the wavelength mainly affected is approxi- mately equal to the beam span. The amplitude of irregu- larity caused by TDL is largely affected by the temperature variation, temperature curve index, and type of temperature gradient, so it is necessary to monitor the temperaturedistribution of bridges in different regions to provide accurate calculation parameters. In order to avoid the irregularity exceeding the limit values, the height of 32, 48, and 64 m simply supported box beam bridges must not be less than 2.15, 3.2, and 4.05 m, respectively.

关键词： Simply supported beam bridge Temperaturegradient Vertical irregularity - Beam height

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Effect of annealing on the characteristics of Au/Ni_(50)Fe_(50) bilayer films grown on glass

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Journal of University of Science and Technology Beijing 2007年第2期14卷 160-166页

作者： Hao Qian Hong Qiu Xiaobai Chen Yue tian ping Wu Fengping wang Liqing Pan Applied Science School University of Science and Technology Beijing Beijing 100083 China Department of Mathematics and Physics Beijing Technology and Business University Beijing 100037 China

Sputter-deposited Au/NisoFeso bilayer films were annealed in a vacuum of 5×10^-4 Pa at 523 to 723 K for 30 or 90 min. The characteristics of the bilayer films were determined by Auger electron spectroscopy, field emission scanning electron microscopy, X- ray diffractometry, a four-point probe technique, and an alternating gradient magnetometer. When the annealing temperature and time reached 723 K and 90 min, Ni and Fe atoms markedly diffused into the Au layer. The grain size of the Au layer did not change markedly with the annealing condition. As the annealing time was 30 min and the annealing temperature exceeded 573 K, the resistance of the bilayer film increased with increasing the annealing temperature. Furthermore, the resistance of the bilayer film annealed at 723 K for 90 ,nin was lower than that of the bilayer film annealed at 723 K for 30 min. All the bilayer films showed magnetic hysteresis loops. The as-deposited bilayer film showed a hard magnetization. The bilayer film represented an easy magnetization with increasing the annealing temperature. The Au/Ni50Fe50 film that annealed at 723 K for 90 min had the lowest saturation magnetization.

关键词： Au/Ni50Fe50 bilayer film annealing diffusion characteristic

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Optimizing hybrid electric vehicle coupling organic Rankine cycle energy management strategy via deep reinforcement learning

Energy and AI

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Energy and AI 2024年第3期17卷 295-312页

作者： Xuanang Zhang Xuan wang ping Yuan Hua tian Gequn Shu State Key Laboratory of Engines Tianjin UniversityNo.92Weijin RoadNankai DistrictTianjin300072China Department of Thermal Science and Energy Engineering University of Science and Technology of ChinaHefei230027China

Trucks consume a lot of energy. Hybrid technology maintains a long range while realizing energy savings. Hybrid is therefore an effective energy-saving technology for trucks. Recovery of engine waste heat through the organic Rankine cycle further enhances engine efficiency and provides effective thermal management. However, the powertrain greatly increases the complexity of energy management system. In order to design an energy management system with high efficiency and robustness, this study proposes a deep reinforcement learning embedded rule-based energy management system. This method optimises the key parameters of the rule-based energy management system by inserting deep reinforcement learning into it. Therefore, this scheme combines the good optimization effect of deep reinforcement learning and the excellent robustness of rule. In order to verify the feasibility of this scheme, this study builds the system dynamic model and carries out a simulation study. Subsequently, a hybrid powertrain semi physical experimental bench was constructed and a rapid control prototype experimental study was carried out. The simulation results show that the deep reinforcement learning embedded rule-based energy management system can reduce the energy consumption by 4.31 % compared with the rule-based energy management system under the C-WTVC driving cycle. In addition, energy saving and safe operation can also be achieved under other unfamiliar untrained driving cycles. The rapid control prototype experimental study shows that the deep reinforcement learning embedded rule-based energy management system has good agreement in experiment and simulation, which demonstrates the potential for real vehicle engineering applications and promotes the engineering application of deep reinforcement learning.

关键词： Deep reinforcement learning Hybrid electric vehicle Organic Rankine cycle Energy management system Rapid control prototype

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Aspirin increases susceptibility of Helicobacter pylori to metronidazole by augmenting endocellular concentrations of antimicrobials

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World Journal of Gastroenterology 2009年第8期15卷 919-926页

作者： Xiao-ping Zhang Wei-Hong wang Yu tian Wen Gao Jiang Li Department of Gastroenterology Peking University First Hospital Beijing 100034 China

AIM： To investigate the increasing the susceptibility pylon） to metronidazole. mechanisms of aspirin of Helicobacter pylori （H METHODS： Hpylori reference strain 26695 and two metronidazole-resistant isolates of H pylori were included in this study. Strains were incubated in Brucella broth with or without aspirin （1 mmol/L）. The rdxA gene of Hpylori was amplified by PCR and sequenced. The permeability of Hpylori to antimicrobials was determined by analyzing the endocellular radioactivity of the cells after incubated with [7-^3H]-tetracycline. The outer membrane proteins （OMPs） of Hpylori 26695 were depurated and analyzed by SDS-PAGE. The expression of 5 porins （hopA, hopB, hopC, hopD and hopE） and the putative RND efflux system （hefABC） of H pylori were analyzed using real-time quantitative PCR. RESULTS： The mutations in rdxA gene did not change in metronidazole resistant isolates treated with aspirin. The radioactivity of H pylori increased when treated with aspirin, indicating that aspirin improved the permeability of the outer membrane of H pylori. However, the expression of two OMP bands between 55 kDa and 72 kDa altered in the presence of *** expression of the mRNA of hopA, hopB, hopC, hopD, hopE and herA, hefB, hefC of H pylori did not change when treated with aspirin. CONCLUSION： Although aspirin increases the susceptibility of H pylori to metronidazole, it has no effect on the mutations of rdxA gene of Hpylori. Aspirin increases endocellular concentrations of antimicrobials probably by altering the OMP expression.

关键词： Helicobacter pylori Aspirin Metronidazole Resistance Minimum inhibitory concentrations

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