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Long non-coding RNA tagging and expression manipulation via CRISPR/Cas9-mediated targeted insertion

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Protein & Cell 2018年第9期9卷 820-825页

作者： tian-lin cheng Zilong Qiu Institute of Neuroscience State Key Laboratory of NeuroscienceCAS Center for Excellence in Brain Science and IntelligenceTechnology Chinese Academy of Sciences Shanghai 200031China

Dear Editor, Long non-coding RNAs （IncRNAs）, defined as RNA transcripts longer than 200 nucleotides without the protein-coding ability （Carninci et al., 2005）, share many features with protein-coding messenger RNAs （mRNAs） such as polyadenylated 5＇ ends and multi-exonic structures （Guttman et al., 2010）. Though expression levels are less abundant, IncRNAs outnumber mRNAs with more diverse regulatory functions （Quinn and Chang, 2016）. They may serve as decoys, sponges, signals or scaffolds in regulating chromatin conformation, nuclear organization, gene expression, and protein activity in cis or trans manner （Ulitsky and Bartel, 2013; Quinn and Chang, 2016）. LncRNAs are involved in various physiological processes and their lossor gain-offunction mutations have been implicated in the pathogenesis of human diseases （Wapinski and Chang, 2011）.

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Density field measurement and approximate reconstruction of supersonic mixing layer

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Chinese Science Bulletin 2010年第19期55卷 2004-2009页

作者： ZHAO YuXin YI ShiHe tian LiFeng HE lin cheng ZhongYu College of Aerospace and Material Engineering National University of Defense Technology Changsha 410073 China

The density field measurement of supersonic mixing layer based on the nanoparticle-based planar laser scattering method was *** calibration of experimental images was analyzed,and the relation between tracer particles concentration and local density of flowfield was calibrated with oblique shockwave *** to the characteristic of mixing layer images,the influence of uneven light intensity distribution was *** these calibration methods,the density field of supersonic mixing layer with convective Mach number at 0.12 was *** analyzing the spanwise vortical structures and streamwise density field,the three dimensional(3D) density field was approximately reconstructed,which apparently reflected the 3D structure of supersonic mixing layer.

关键词：超音速混合层密度场测量超声速图像校准实验校准三维结构激光散射法

Role of the PTEN signaling pathway in autism spectrum disorder

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Neuroscience Bulletin 2013年第6期29卷 773-778页

作者： Jing-Wen Lv tian-lin cheng Zi-Long Qiu Wen-Hao Zhou Department of Neonatology Children's Hospital of Fudan University Institute of Neuroscience Shanghai Institute of Biological SciencesChinese Academy of Sciences

Autism is an etiologically heterogeneous group of neurodevelopmental disorders,diagnosed mostly by the clinical behavioral *** concept that the tumor-related gene PTEN plays a critical role in autism spectrum disorder has emerged over the last *** this review,we focus on the essential role of the PTEN signaling pathway in neuronal differentiation and the formation of neural circuitry,as well as genetic mouse models with Pten ***,accumulated data suggest that the effect of PTEN on neural stem-cell development contributes significantly to the pathophysiology of autism spectrum disorders.

关键词： PTEN TSC1/2 autism synapse neural stem cells

CFD Validation of the Optimal Arrangement of the Propulsive Dorsal Fin of Gymnarchus niloticus

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Journal of Bionic Engineering 2006年第3期3卷 139-146页

作者： Hu tian-jiang Shen lin-cheng Gong Pei-ling Institute of Automation College of Mechatronic Engineering and Automation National University of Defense Technology Changsha 410073 P. R. China

Gymnarchus niloticus, a typical freshwater fish, swims by undulations of a long-based dorsal fin aided by the two pectoral fins, while commonly it holds its body rigid and straight. The long flexible dorsal fin is the main propulsor of G niloticus; it has also considerable influence on the streamline profile. This paper proposes a CFD approach to validate that the natural arrangement of the propulsive dorsal fin is optimal. Using morphological data and a smoothness-keeping algorithm, the dorsal fin is ‘virtually＇ moved forward and backward with several displacements from the natural location. For each case, we reconstruct geometry, generate CFD grids, and calculate the pressure, viscous and total drag coefficients respectively. The results show that the pressure and total drag coefficients increase whether the dorsal fin is displaced forward or backward, and that greater displacement from its original position leads to greater pressure and total drag coefficients. This suggests that the natural position of the dorsal fin is significant for maintaining the fish＇s streamline profile and reducing drag.

关键词： Gymnarchus niloticus CFD validation smoothness-keeping algorithm streamline profile drag coefficients

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Journal of Genetics and Genomics 2017年第1期44卷 63-66页

作者： BO Yuan tian-lin cheng Kan Yang Xu Zhang Zilong Qiu Institute of Neuroscience Key Laboratory of Primate Neurobiology CAS Center for Excellence in Brain Science and Intelligence Technology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Institute of Chemistry and Bioengineering Donghua University Shanghai 200051 China Shanghai Clinical Research Center Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Methyl-CpG binding protein 2 （MeCP2） has a crucial role in transcriptional regulation and neural development （Ausi6 et al., 2014）. Loss of function mutations of MECP2 in human lead to Rett syndrome （RTT）, a severe neurodevelopmental disorders （Amir et al., 1999）, whereas individuals with the chromosomal duplications containing the MECP2 locus showed severe autism-like symptoms （Ramocki et al., 2009）.

关键词： FGF Autism-related protein MeCP2 regulates FGF13 expression and emotional behaviors microRNAs

Dural enhancement detected by magnetic resonance imaging reflecting the underlying causes of cerebral venous sinus thrombosis

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Chinese Medical Journal 2012年第8期125卷 1513-1516页

作者： tian cheng-lin PU Chuan-qiang Department of Neurology Chinese People's Liberation Army General HospitalBeijing 100853China

Dural enhancement detected by magnetic resonance imaging is a common finding in patients with cerebral venous sinus thrombosis （CVST） and is usually interpreted as a change secondary to CVST. We report two cases of CVST with intense and diffuse dural enhancement that resulted from pachymeningitis in one patient and spontaneous intracranial hypotension in another. Pachymeningitis and spontaneous intracranial hypotension were also determined to be the underlying causes of CVST. The clinical data of these two patients are described. In patients with CVST, dural enhancement is not always a secondary change to CVST. It can be a manifestation of the underlying causes of CVST. When diffuse and intense dural enhancement is revealed, sufficient ancillary tests are warranted to rule out other potential patholooical chanoes of the dura mater those can result in CVST.

关键词： cerebral venous sinus thrombosis magnetic resonance imaging pachymeningitis spontaneous intracranial hypotension

An experimental study of aero-optical aberration and dithering of supersonic mixing layer via BOS

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Science China(Physics,Mechanics & Astronomy) 2010年第1期53卷 81-94页

作者： ZHAO YuXin , YI ShiHe, tian LiFeng, HE lin & cheng ZhongYu College of Aerospace and Material Engineering, National University of Defense Technology, Changsha 410073, China College of Aerospace and Material Engineering National University of Defense Technology Changsha China

The optical performance of supersonic mixing layer is heavily deteriorated by the aero-optical aberration and dithering of coherent structures, but current measuring methods limit the spatiotemporal resolution in relevant studies. A high resolution whole-field aero-optical aberration and dithering measuring method based on the Background Orient Schlieren (BOS) technique was studied. The systematic structure, sensitivity and resolution of BOS are analyzed in this paper. The aero-optical aberration and dithering of streamwise structures in supersonic mixing layers were quantificationally studied with BOS. The aberration field of spanwise structures revealed the ribbon-like aberration structures, which heavily restrict the optical performance of a mixing layer. The quantifications of aero-optical aberration and dithering are very important in studying aero-optical performance of supersonic mixing layer.

关键词： aero-optics supersonic mixing layer BOS

Multiresolution analysis of density fluctuation in supersonic mixing layer

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Science China(Technological Sciences) 2010年第2期53卷 584-591页

作者： ZHAO YuXin1, YI ShiHe, tian LiFeng, HE lin & cheng ZhongYu College of Aerospace and Material Engineering, National University of Defense Technology, Changsha 410073, China College of Aerospace and Material Engineering National University of Defense Technology Changsha China

Due to the difficulties in measuring supersonic density field, the multiresolution analysis of supersonic mixing layer based on experimental images is still a formidable challenge. By utilizing the recently developed nanoparticle based planar laser scattering method, the density field of a supersonic mixing layer was measured at high spatiotemporal resolution. According to the dynamic behavior of coherent structures, the multiresolution characteristics of density fluctuation signals and density field images were studied based on Taylor’s hypothesis of space-time conversion and wavelet analysis. The wavelet coefficients reflect the characteristics of density fluctuation signals at different scales, and the detailed coefficients reflect the differences of approximation at adjacent levels. The density fluctuation signals of supersonic mixing layer differ from the periodic sine signal and exhibit similarity to the fractal Koch signal. The similarity at different scales reveals the fractal characteristic of mixing layer flowfield. The two-dimensional wavelet decomposition and reconstruction of density field images extract the approximate and detailed signals at different scales, which effectively resolve the characteristic structures of the flowfield at different scales.

关键词： supersonic mixing layer density fluctuation multiresolution analysis

Approximate Gaussian conjugacy： parametric recursive filtering under nonlinearity, multimodality, uncertainty, and constraint, and beyond

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Frontiers of Information Technology & Electronic Engineering 2017年第12期18卷 1913-1939页

作者： tian-cheng lin Jin-ya SU Wci LIU Juan M. CORCHADO School of Sciences University of Salamanca Salamanca 37007 Spain Department of Aeronautical and Automotive Engineering Loughborough University Loughborough LE11 3TU UK De~artment of Electronic and Electrical En.oineering University of She3ff~eld She3ff~eld $1 ~ET UK

Since the landmark work of R. E. Kalman in the 1960s, considerable efforts have been devoted to time series state space models for a large variety of dynamic estimation problems. In particular, parametric filters that seek analytical estimates based on a closed-form Markov-Bayes recursion, e.g., recursion from a Gaussian or Gaussian mixture （GM） prior to a Gaussian/GM posterior （termed ＇Gaussian conjugacy＇ in this paper）, form the backbone for a general time series filter design. Due to challenges arising from nonlinearity, multimodality （including target maneuver）, intractable uncertainties （such as unknown inputs and/or non-Gaussian noises） and constraints （including circular quantities）, etc., new theories, algorithms, and technologies have been developed continuously to maintain such a conjugacy, or to approximate it as close as possible. They had contributed in large part to the prospective developments of time series parametric filters in the last six decades. In this paper, we review the state of the art in distinctive categories and highlight some insights that may otherwise be easily overlooked. In particular, specific attention is paid to nonlinear systems with an informative observation, multimodal systems including Gaussian mixture posterior and maneuvers, and intractable unknown inputs and constraints, to fill some gaps in existing reviews and surveys. In addition, we provide some new thoughts on alternatives to the first-order Markov transition model and on filter evaluation with regard to computing complexity.

关键词： Kalman filter Gaussian filter Time series estimation Bayesian filtering Nonlinear filtering Constrained filtering Gaussian mixture Maneuver Unknown inputs