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T-2 toxin-induced apoptosis involving Fas,p53,Bcl-xL,Bcl-2,Bax and caspase-3 signaling pathways in human chondrocytes

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第6期9卷 455-463页

作者： Jing-hong CHEN Jun-ling CAO Yong-lie CHU Zhi-lun wang Zhan-tian YANG hong-lin wang Ministry of Education Key Laboratory of Environment and Genes related to Diseases Institute of Endemic Diseases School of Medicine Xi ＇an Jiaotong University Xi ＇an 710061 China

Objective:To investigate the effects of T-2 toxin on expressions of Fas,p53,Bcl-xL,Bcl-2,Bax and caspase-3 on human ***:Human chondrocytes were treated with T-2 toxin(1～20 ng/ml)for 5 ***,p53 and other apoptosis-related proteins such as Bax,Bcl-2,Bcl-xL,caspase-3 were determined by Western blot analysis and their mRNA expressions were determined by reverse transcriptase-polymerase chain reaction(RT-PCR).Results:Increases in Fas,p53 and the pro-apoptotic factor Bax protein and mRNA expressions and a decrease of the anti-apoptotic factor Bcl-xL were observed in a dose-dependent manner after exposures to 1～20 ng/ml T-2 toxin,while the expression of the anti-apoptotic factor Bcl-2 was ***,T-2 toxin could also up-regulate the expressions of both pro-caspase-3 and caspase-3 in a dose-dependent ***:These data suggest a possible underlying molecular mechanism for T-2 toxin to induce the apoptosis sig- naling pathway in human chondrocytes by regulation of apoptosis-related proteins.

关键词： Apoptosis Apoptosis-related proteins Chondrocyte T-2 toxin

Doubly-charged scalar in four-body decays of neutral flavored mesons

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Chinese Physics C 2019年第1期43卷 16-23页

作者： tian-hong wang Geng Li Yue Jiang Guo-Li wang Department of Physics Harbin Institute of Technology

Abstract:We study the four-body decays of neutral flavored mesons,including■,and■.These processes,which could be induced by a hypothetical doubly-charged scalar particle,do not conserve the lepton ***,as an example,that the mass of the doubly-charged particle is 1000 Ge V,and using the upper bounds of the couplings,we calculate the branching ratios of different ***■,and■,it is of the order of 10^(-30),10^(-32)–10^(-29),and 10^(-33)–10^(-28),*** on the experimental results for the■channels,we also find the upper limit for the quantity■.

关键词： doubly-charged scalar four-body decay neutral flavored meson

Clinicopathological features of atypical lipomatous tumors of the laryngopharynx

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第12期11卷 918-922页

作者： Huai-yin SHI Li-xin WEI hong-tian wang Lu SUN Department of Pathology Chinese PLA General Hospital Beijing 100853 China Department of Otorhinolaryngology Chinese PLA General Hospital Beijing 100853 China

Atypical lipomatous tumor (ALT) of the laryngopharynx is *** we report five cases to demonstrate their clinicopathological *** patients were four males and one female,aged 41 to 69 years (median 53.6 years).All tumors (two in the hypopharynx and three in the larynx) presented as a slowly growing,painless *** included dysphagia (2/5),dysphonia (3/5),and the feeling of a foreign body in the throat (5/5).Tumors were well circumscribed or focally infiltrative,ranging from 2.0 to 5.0 cm (median,3.4 cm) in size,and microscopically showed the typical features of lipoma-like ***,tumor cells were stained with S-100,vimentin,murine double minute 2 (MDM-2),and cyclin-dependent kinase 4 (CDK4).Two patients had local tumor recurrences at 6 and 14 months after initial surgery during *** of laryngopharynx is an indolent *** staining for MDM-2 and CDK4 is helpful in pathological diagnosis.

关键词： Atypical lipomatous tumor (ALT) Laryngopharynx Immunohistochemistry

Impact of Mo/Ni alloying on microstructural modulation and low-temperature toughness of high-strength low-alloy steel

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Journal of Iron and Steel Research International 2024年第7期31卷 1746-1762页

作者： Wei Liu hong-li Zhao Bing-xing wang Yong tian State Key Laboratory of Rolling and Automation Northeastern UniversityShenyang 110819LiaoningChina

The high-strength low-alloy steel plates with varying Ni/Mo contents were manufactured using the thermos-mechanical control *** investigation was conducted to explore the effect of Ni/Mo microalloying on microstructure evolution and mechanical properties of the *** results revealed that the increase in Ni content from 1 to 2 wt.%reduced the transition temperature of ferrite and the growth range of ferritic grain was narrowed,which promoted grain *** optimized combination of grain size,high-angle grain boundaries(HAGBs),and martensite-austenite(M-A)islands parameter contributed to the excellent impact toughness of S1 steel at-100℃(impact absorbed energy of 218.2 J at-100℃).As the Mo increases from 0 to 2 wt.%,the matrix structure changes from multiphase structure to granular bainite,which increases the average effective grain size to~4.62 pm and reduces HAGBs proportion to~36.22%.With these changes,the low-temperature impact toughness of S3 steel is *** addition,based on the analysis of the characteristics of crack propagation path,it was found that M-A islands with low content(~2.21%)and small size(~1.76 pm)significantly retarded crack propagation,and the fracture model of M-A islands with different morphologies was further ***,correlation between behaviour of delamination and toughness was further analysed by observing delamination size and impact energy parameters.

关键词： High-strength low-alloy steel Microstructural regulation Ni addition Mo addition Crack propagation Low-temperature toughness

Strong decays of P-wave mixing heavy-light 1^+ states

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Chinese Physics C 2018年第12期42卷 1-8页

作者： Zhi-Hui wang Yi Zhang tian-hong wang Yue Jiang Qiang Li Guo-Li wang School of Electrical &: Information Engineering North Minzu University Yinchuan 750021 China Department of Physics Harbin Institute of Technology Harbin 150001 China Institute of Theoretical Physics Chinese Academy of Sciences Beijing 100190 China

Many P-wave mixing heavy-light 1^＋ states have not yet been discovered by experiment, while others have been discovered but without width information, or with large uncertainties on the widths. In this paper, the strong decays of the P-wave mixing heavy-light 1^＋ states D^0, D^±, D^±, B^0, B^±： and Bs are studied by the improved Bethe SMpeter （B-S） method with two conditions of mixing angle 0： one is 0 = 35.3°; the other is considering a correction to the mixing angle 0=35.3°＋θ1. Valuable predictions for the strong decay widths are obtained： F（D1^0）=232 MeV, r（D1^0）=21.5 MeV, F（D1^±）=232 MeV, F（D1^±）=21.5 MeV, F（Ds1^±）=0.0101 MeV,Г（Ds1^±l）=0.950 MeV, F（BГ^±＋）=263 MeV, F（B1^±=16.8 MeV, F（B′s1）=0.01987 MeV and F（B,1）=0.412 MeV. It is found that the decay widths of D~ and Bsl are very sensitive to the mixing angle. The results will provide theoretical assistance to future experiments.

关键词： P-wave mixing states strong decay mixing angle improved B-S method

Novel Cellulase Profile of Trichoderma reesei Strains Constructed by cbh1 Gene Replacement with eg3 Gene Expression Cassette

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Acta Biochimica et Biophysica Sinica 2004年第10期36卷 667-672页

作者： tian-hong wang*, Ti LIU, Zhi-hong WU, Shi-Li LIU, Yi LU, and Yin-Bo QU The State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China The State Key Laboratory of Microbial Technology Shandong UniversityJinan 250100 China

To construct strains of the filamentous fungus Trichoderma reesei with low cellobiohydrolases while high endoglucanase activity, the Pcbh1-eg3-Tcbh1 cassette was constructed and the coding sequence of the cellobiohydrolase I (CBHI) gene was replaced with the coding sequence of the eg3 gene by homologous recombination. Disruption of the cbh1 gene was confirmed by PCR, Southern dot blot and Western hybri- dization analysis in two transformants denoted as L13 and L29. The filter paper-hydrolyzing activity of strain L29 was 60% of the parent strain Rut C30, and the CMCase activity was increased by 33%. This relatively modest increase suggested that the eg3 cDNA under the control of the cbh1 promoter was not efficiently transcribed as the wild type cbhl gene. However our results confirmed that homologous recombination could be used to construct strains of the filamentous fungus Trichoderma reesei with novel cellulase profile. Such strains are of interest from the basic science perspective and also have potential industrial applications.

关键词： Trichoderma reesei gene replacement gene disruption cbh1 eg3

High-throughput calculations combining machine learning to investigate the corrosion properties of binary Mg alloys

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Journal of Magnesium and Alloys 2024年第4期12卷 1406-1418页

作者： Yaowei wang tian Xie Qingli Tang Mingxu wang Tao Ying hong Zhu Xiaoqin Zeng University of Michigan-Shanghai Jiao Tong University Joint Institute Shanghai Jiao Tong University200240ShanghaiChina State Key Laboratory of Metal Matrix Composites Shanghai Jiao Tong UniversityShanghai200240China

Magnesium(Mg)alloys have shown great prospects as both structural and biomedical materials,while poor corrosion resistance limits their further *** this work,to avoid the time-consuming and laborious experiment trial,a high-throughput computational strategy based on first-principles calculations is designed for screening corrosion-resistant binary Mg alloy with intermetallics,from both the thermodynamic and kinetic *** stable binary Mg intermetallics with low equilibrium potential difference with respect to the Mg matrix are firstly ***,the hydrogen adsorption energies on the surfaces of these Mg intermetallics are calculated,and the corrosion exchange current density is further calculated by a hydrogen evolution reaction(HER)kinetic *** intermetallics,e.g.Y_(3)Mg,Y_(2)Mg and La_(5)Mg,are identified to be promising intermetallics which might effectively hinder the cathodic ***,machine learning(ML)models are developed to predict Mg intermetallics with proper hydrogen adsorption energy employing work function(W_(f))and weighted first ionization energy(WFIE).The generalization of the ML models is tested on five new binary Mg intermetallics with the average root mean square error(RMSE)of 0.11 *** study not only predicts some promising binary Mg intermetallics which may suppress the galvanic corrosion,but also provides a high-throughput screening strategy and ML models for the design of corrosion-resistant alloy,which can be extended to ternary Mg alloys or other alloy systems.

关键词： Mg intermetallics Corrosion property High-throughput Density functional theory Machine learning

Genomic Diversity and Evolution of Bacillus subtilis

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Biomedical and Environmental Sciences 2015年第8期28卷 620-625页

作者： YU Gang wang Xun Cheng tian wang hong SHI Ji Chun wang Bin YE Qiang DONG Si Guo ZENG Ming wang Jun Zhi The State Key Laboratory of Cancer Biology Department of Biopharmaceutics School of Pharmacy Fourth Military Medical University Division of Enteric Bacterial Vaccines National Institutes for Food and Drug Control Tsinghua-Peking Joint Center for Life Sciences School of Life Sciences Tsinghua university

Bacillus subtilis is the focus of both academic and industrial *** studies have reported a number of sequence variations in different *** *** uncover the genetic variation and evolutionary pressure in *** strains,we performed whole genome sequencing of two *** isolates,KM and *** genomic analyses of these two strains with other *** strains identified high

关键词： gene SNPs

BLUPmrMLM:A Fast mrMLM Algorithm in Genome-wide Association Studies

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Genomics, Proteomics & Bioinformatics 2024年第3期22卷 55-67页

作者： hong-Fu Li Jing-tian wang Qiong Zhao Yuan-Ming Zhang College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China

Multilocus genome-wide association study has become the state-of-the-art tool for dissecting the genetic architecture of complex and multiomic ***,most existing multilocus methods require relatively long computational time when analyzing large *** address this issue,in this study,we proposed a fast mrMLM method,namely,best linear unbiased prediction multilocus random-SNP-effect mixed linear model(BLUPmrMLM).First,genome-wide single-marker scanning in mrMLM was replaced by vectorized Wald tests based on the best linear unbiased prediction(BLUP)values of marker effects and their variances in ***,adaptive best subset selection(ABESS)was used to identify potentially associated markers on each chromosome to reduce computational time when estimating marker effects via empirical ***,shared memory and parallel computing schemes were used to reduce the computational *** simulation studies,BLUPmrMLM outperformed GEMMA,EMMAX,mrMLM,and FarmCPU as well as the control method(BLUPmrMLM with ABESS removed),in terms of computational time,power,accuracy for estimating quantitative trait nucleotide positions and effects,false positive rate,false discovery rate,false negative rate,and F1 *** the reanalysis of two large rice datasets,BLUPmrMLM significantly reduced the computational time and identified more previously reported genes,compared with the aforementioned *** study provides an excellent multilocus model method for the analysis of large-scale and multiomic *** software mrMLM v5.1 is available at BioCode(https://***/biocode/tool/BT007388)or GitHub(https://***/YuanmingZhang65/mrMLM).

关键词： Genome-wide association study BLUP Multilocus model mrMLM Large-scale dataset