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A zebrafish gene trap line expresses GFP recapturing expression pattern of foxj1b

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Journal of Genetics and Genomics 2009年第10期36卷 581-589页

作者： tian tian Long Zhao Xinyi Zhao Min Zhang Anming Meng Protein Science Laboratory of Ministry of Education Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China State Key Laboratory of Biomembrane and Membrane Engineering Institute of Zoology Chinese Academy of Sciences Beijing 100101 China

Foxj1 has been found to play an important role in cilia formation and function in vertebrates. The zebrafish or Xenopus genome expresses two Foxjl genes, foxjlalFoxJ1 and foxjlb/FoxJ1.2. In this study, we have generated a zebrafish transgenic line T2BGSZIO by Tol2 transposon-based gene trapping approach. T2BGSZ10 transgenic fish carry an insertion of the transposon genome into the first intron of thefoxj1b locus. This insertion results in GFP expression in the forebrain, otic vesicles, floorplate, pronephric ducts and other domains during embryogenesis, which recaptures the expression pattern offoxj1b. Although normal expression offoxj1b is dramatically reduced, T2BGSZ10 homozygous embryos develop normally and grow to adulthood without detectable defects, which may be due to the incomplete interruption of foxjlb expression. Nevertheless, this transgenic line may serve as a useful model for dynamic observation of GFP-labeled tissues and organs and for isolation of GFP-labeled cells.

关键词： zebrafish transposon embryo transgenic foxj1

The Dynamical Casimir Effect versus Collective Excitations in Atom Ensemble

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Chinese Physics Letters 2011年第6期28卷 118-121页

作者： ZHANG Xue ZHENG Tai-Yu tian tian PAN Shu-Mei Institute of Theoretical Physics Northeast Normal UniversityChangchun 130024

The Dynamical Casimir effect and collective excitations in atom ensemble are investigated in a non-stationary *** results show that the dynamical Casimir effect leads to the collective excitations of atom ensemble due to absorption of the created *** remarkable feature indicates that the atom ensemble can serve as a detector for the process of photon production in a non-stationary *** by measuring the collective excitations of the atom ensemble,the dynamical Casimir effect can indirectly be verified.

关键词： collective ensemble excitation

GAG-containing nucleotides as mediators of DNA-silver clusters and iron-DNA interplay

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Chinese Chemical Letters 2016年第3期27卷 395-398页

作者： tian-tian Chen Qiu-Yun Chen Ming-Yang Liu School of Chemistry and Chemical Engineering Jiangsu University State Key Laboratory of Coordination Chemistry Nanjing University

New types of fluorescence DNA-based silver nanoclusters（DNAn-Ag NCs, n = 1, 2, 3c, 4c, 5c） were synthesized by C3T-rich nucleotides as templates. It is found that the assembly of DNAn-Ag NCs with nucleotides containing GAG sequences produce silver clusters with an enhanced red emission. Results indicate that GAG is the good enhancer of DNAn-Ag NCs constructed by C3T-rich nucleotides. The fluorescence titration reveals that enhanced red emission is sensitive to Fe（Ⅲ/Ⅱ） ions with the formation of non-emission nanoclusters. Thus, the GAG-containing nucleotide can be an enhancer for the emission of silver clusters with C3T-rich nucleotide and a mediator of the iron-cluster interplay.

关键词： DNA Silver cluster Fluorescence Iron

The synthesis,characterization and X-ray diffractometry structure of fluorine-containing cyclobis(paraquat-p-phenylene) tetracation

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Chinese Chemical Letters 2010年第6期21卷 633-636页

作者： Hai Tao Xi tian tian Yi Xiao Qiang Sun Key Laboratory of Fine Petrochemical Engineering Jiangsu Polytechnic UniversityChangzhou 213164China

Two novel fluorine-containing cyclophanes 1 and 2 incorporating a 1,4-bismethylfluorophenylene subunits have been synthesized via the template-directed clipping methodology and their structure was characterized by ~1H NMR,^(13)C NMR, MALDI-TOF-MS and elemental analysis.X-ray diffraction analysis of the solid state molecular structure showed that the complex units are linked by non-classical C-H…F hydrogen bonding.

关键词： Fluorine-cotiaining cyclophanes 1,4-Bis(bromomethyl)-2-fluorobenzene Crystal structure Synthesis

Mechanism of lithium insertion into NiSi_2 anode material for lithium ion batteries

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Rare Metals 2006年第Z1期25卷 77-81页

作者： WEN Zhongsheng JI Shijun SUN Juncai tian Feng tian Rujin XIE Jingying Institute of Materials and Technology Dalian Maritime University Dalian 116026 China Laboratory of Energy Science and Technology Shanghai Institude of Microsystem and Information Technology Chinese Academy of Sciences Shanghai 200050 China Institute of Materials and Technology Dalian Maritime University Dalian 116026 China Institute of Materials and Technology Dalian Maritime University Dalian 116026 China Institute of Materials and Technology Dalian Maritime University Dalian 116026 China Institute of Materials and Technology Dalian Maritime University Dalian 116026 China Laboratory of Energy Science and Technology Shanghai Institude of Microsystem and Information Technology Chinese Academy of Sciences Shanghai 200050 China

As a promising high capacity anode material for lithium ion batteries, the lithium insertion performance and possible insertion mechanism of binary alloy of NiSi2 were discussed. The initial lithium insertion of crystal NiSi2 can reach up to 600 mAh·g-1, but large irreversible capacity occurrs simultaneously for serious structure transformation and the irreversible phase forms. XRD and XPS were employed to detect the crystal structure and composition changes produced by lithium insertion. The lithium insertion-extraction behavior of NiSi2 electrode is similar to that of silicon after the first discharge. The structure stability seems related to the non-stoichimometric Ni-Si compound formed by lithium insertion into NiSi2.

关键词： anode lithium ion batteries nickel silicide high capacity

Internal layering structure and subglacial conditions along a traverse line from Zhongshan Station to Dome A,East Antarctica,revealed by ground-based radar sounding

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Applied Geophysics 2020年第5期17卷 870-878,905页

作者： Tang Xue-Yuan Sun Bo Wang tian-tian MNR Key Laboratory for Polar Science Polar Research Institute of ChinaShanghai 200136China

During the 21st Chinese National Antarctic Research Expedition(CHINARE 21,2004/05),a radar dataset was collected using a ground-based radar system,along a traverse line from Zhongshan Station to DT401(130 km from the Kunlun station).The internal layering structure and subglacial conditions were revealed along the radar profi *** internal layers,disturbed layers,and echo-free zones(EFZs)along the profi le were identifi ed and classifi ed,and the spatial distribution was *** on recent surface ice velocity data,we found that the internal layers at a depth of 200-300 m in the upper ice sheet are continuous,smooth,and nearly parallel to the ice surface *** addition,the thick band of continuous layers changes little with increasing *** depths below 300 m,the geometric structure of the internal layers and the vertical width of the EFZ band are infl uenced by the surface ice velocity and bed *** relatively high disturbance,layer discontinuity,and larger EFZ band width directly correspond to a higher surface ice velocity and a sharper bed *** particular,we found that at a depth of 650-950 km,the Lambert Glacier Rift in the Gamburtsev Mountains has a higher ice fl ow;moreover,the revealed internal layers are disturbed or broken,and the maximal vertical width of the EFZ band most likely exceeds 2000 m.

关键词： East Antarctic ice sheet Dome A Radar Internal layers Subglacial conditions

β-Cyclodextrin carbon-based nanoparticles with a core-shell-shell structure for efficient adsorption of crystal violet and bisphenol A

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Particuology 2022年第3期20卷 88-97页

作者： tian tian Mengling Liu Yinhui Li Jian Han Liang Ren Heike Lorenz Qing Wu Jianxin Chen School of Chemical Engineering and Technology Hebei University of TechnologyTianjin 300130China MDPI(tianjin)Technology Services Co. Ltd.Tianjin 300130China Max-Planck-Institut für Dynamik komplexer technischer Systeme Sandtorstrasse 139106 MagdeburgGermany School of Artificial Intelligence Hebei University of TechnologyTianjin 300130China

Textile wastewater contains highly toxic,nondegradable,carcinogenic organic pollutants,causing severe damage to the ecological *** this work,β-cyclodextrin carbon-sphere-based nanocomposite(CS/SiO_(2)@β-CD)with a core-shell-shell configuration was prepared by hydrothermal synthesis and subsequent sedimentation-azeotropic distillation with SiO_(2)as intermediate layer for the removal of crystal violet(CV)and bisphenol A(BPA).The chemical structure and morphology of the CS/SiO_(2)@β-CD were characterized by FT-IR,SEM and *** effects of contact time,pH,temperature and initial concentration of the absorbates on the adsorption property were *** CS/SiO_(2)@β-CD showed excellent adsorption capacity on CV and BPA,and the maximum adsorption capacity was 87.873 mg g^(-1)and 165.095 mg g^(-1),*** adsorption process was in line with the pseudo-second-order and pseudo-first-order kinetic models,*** adsorption thermodynamics could be better fitted by Langmuir model than by Freundlich *** calculated thermodynamic parameters(-ΔG0,ΔS0 andΔH0)illustrated the adsorption of CV was endothermic,in contrast,the adsorption of BPA was exothermic ***,the CS/SiO_(2)@β-CD showed excellent recovery performance,making it a potential adsorbent for the practical treatment of textile wastewater.

关键词： Cyclodextrin Adsorption Textile wastewater Kinetic and thermodynamic models

NADPH homeostasis in cancer:functions,mechanisms and therapeutic implications

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Signal Transduction and Targeted Therapy 2020年第1期5卷 518-529页

作者： Huai-Qiang Ju Jin-Fei Lin tian tian Dan Xie Rui-Hua Xu State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer MedicineSun Yat-sen University Cancer CenterSun Yat-sen University510060 GuangzhouChina Research Unit of Precision Diagnosis and Treatment for Gastrointestinal Cancer Chinese Academy of Medical Sciences510060 GuangzhouChina

Nicotinamide adenine dinucleotide phosphate(NADPH)is an essential electron donor in all organisms,and provides the reducing power for anabolic reactions and redox *** homeostasis is regulated by varied signaling pathways and several metabolic enzymes that undergo adaptive alteration in cancer *** metabolic reprogramming of NADPH renders cancer cells both highly dependent on this metabolic network for antioxidant capacity and more susceptible to oxidative *** the unique NADPH homeostasis of cancer cells might be an effective strategy to eliminate these *** this review,we summarize the current existing literatures on NADPH homeostasis,including its biological functions,regulatory mechanisms and the corresponding therapeutic interventions in human cancers,providing insights into therapeutic implications of targeting NADPH metabolism and the associated mechanism for cancer therapy.

关键词： homeostasis therapeutic metabolism

Catalyst-free and Reprocessable Aromatic Polydithiourethanes

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Chinese Journal of Polymer Science 2024年第10期42卷 1435-1441,I0007页

作者： Bo Yang Hai-Jun Feng tian-tian Ni Xiao-Rui Zhou Tao Xie Ning Zheng State Key Laboratory of Chemical Engineering College of Chemical and Biological EngineeringZhejiang UniversityHangzhou310058China Department of Colorectal Surgery and Oncology Key Laboratory of Cancer Prevention and InterventionMinistry of EducationThe Second Affiliated HospitalZhejiang University School of MedicineHangzhou310009China

The incorporation of dynamic covalent bonds into thermosets facilitates the reprocessing of polymer networks,thereby meeting the sustainable requirements for polymer ***,the mechanical properties of many materials often decline significantly upon reprocessing due to side reactions caused by harsh processing *** this work,we find that the aromatic dithiocarbamate bond can undergo dissociation under mild conditions without the need for a catalyst,enabling the efficient reprocessing of the corresponding *** a consequence,the mechanical properties of the polydithiourethane can be largely preserved after *** discovery of this dynamic chemistry is anticipated to broaden the potential for material design in dynamic covalent polymer networks.

关键词： Reprocessing Polydithiourethane Dynamic covalent bond