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分离纯化重组解脂耶氏酵母发酵液中虫草素的研究

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食品与发酵工业 2025年第1期51卷 26-31页

作者：宋丽萍段希宇王翀卢向阳田云湖南农业大学生物科学技术学院湖南长沙410128 沅江市芦小妹食品有限公司湖南益阳413000

多项研究证明以解脂耶氏酵母作为虫草素生物合成的细胞工厂,是获得虫草素的有效途径。为获得下游虫草素分离纯化工艺,该研究采用不同规格的大孔吸附树脂对重组解脂耶氏酵母发酵液中的虫草素进行静态、动态纯化。结果显示,以NKA-Ⅱ大孔... 详细信息

多项研究证明以解脂耶氏酵母作为虫草素生物合成的细胞工厂,是获得虫草素的有效途径。为获得下游虫草素分离纯化工艺,该研究采用不同规格的大孔吸附树脂对重组解脂耶氏酵母发酵液中的虫草素进行静态、动态纯化。结果显示,以NKA-Ⅱ大孔吸附树脂作为吸附材料,在虫草素质量浓度为3 mg/mL、pH值为9的上样液以上样流速为3 BV/h、上样体积为10 BV、洗杂流速为2 BV/h、洗杂体积为3 BV、洗脱剂为体积分数为30%乙醇、洗脱流速为3 BV/h、洗脱体积为15 BV时,发酵液中虫草素回收率可达到87.67%,纯度达65.82%。该研究为今后利用重组解脂耶氏酵母大规模生产制备虫草素提供数据参考。

关键词：虫草素解脂耶氏酵母分离纯化大孔吸附树脂甘蔗糖蜜

Clinicopathological features of atypical lipomatous tumors of the laryngopharynx

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第12期11卷 918-922页

作者： Huai-yin SHI Li-xin WEI hong-tian WANG lu SUN Department of Pathology Chinese PLA General Hospital Beijing 100853 China Department of Otorhinolaryngology Chinese PLA General Hospital Beijing 100853 China

Atypical lipomatous tumor (ALT) of the laryngopharynx is *** we report five cases to demonstrate their clinicopathological *** patients were four males and one female,aged 41 to 69 years (median 53.6 years).All tumors (two in the hypopharynx and three in the larynx) presented as a slowly growing,painless *** included dysphagia (2/5),dysphonia (3/5),and the feeling of a foreign body in the throat (5/5).Tumors were well circumscribed or focally infiltrative,ranging from 2.0 to 5.0 cm (median,3.4 cm) in size,and microscopically showed the typical features of lipoma-like ***,tumor cells were stained with S-100,vimentin,murine double minute 2 (MDM-2),and cyclin-dependent kinase 4 (CDK4).Two patients had local tumor recurrences at 6 and 14 months after initial surgery during *** of laryngopharynx is an indolent *** staining for MDM-2 and CDK4 is helpful in pathological diagnosis.

关键词： Atypical lipomatous tumor (ALT) Laryngopharynx Immunohistochemistry

Electrons dynamics control by shaping femtosecond laser pulses in micro/nanofabrication: modeling, method, measurement and application

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Light(Science & Applications) 2017年第1期6卷 249-275页

作者： Lan Jiang An-Dong Wang Bo Li tian-hong Cui Yong-Feng lu Laser Micro/Nano-Fabrication Laboratory School of Mechanical EngineeringBeijing Institute of TechnologyBeijing 100081China Department of Mechanical Engineering University of MinnesotaMinneapolisMN 55455USA Department of Electrical Engineering University of Nebraska-LincolnLincolnNE 68588-0511USA

During femtosecond laser fabrication,photons are mainly absorbed by electrons,and the subsequent energy transfer from electrons to ions is of picosecond ***,lattice motion is negligible within the femtosecond pulse duration,whereas femtosecond photon-electron interactions dominate the entire fabrication ***,femtosecond laser fabrication must be improved by controlling localized transient electron dynamics,which poses a challenge for measuring and controlling at the electron level during fabrication ***-probe spectroscopy presents a viable solution,which can be used to observe electron dynamics during a chemical *** fact,femtosecond pulse durations are shorter than many physical/chemical characteristic times,which permits manipulating,adjusting,or interfering with electron ***,we proposed to control localized transient electron dynamics by temporally or spatially shaping femtosecond pulses,and further to modify localized transient materials properties,and then to adjust material phase change,and eventually to implement a novel fabrication *** review covers our progresses over the past decade regarding electrons dynamics control(EDC)by shaping femtosecond laser pulses in micro/nanomanufacturing:(1)Theoretical models were developed to prove EDC feasibility and reveal its mechanisms;(2)on the basis of the theoretical predictions,many experiments are conducted to validate our EDC-based femtosecond laser fabrication *** examples are reported,which proves that the proposed method can significantly improve fabrication precision,quality,throughput and repeatability and effectively control micro/nanoscale structures;(3)a multiscale measurement system was proposed and developed to study the fundamentals of EDC from the femtosecond scale to the nanosecond scale and to the millisecond scale;and(4)As an example of practical applications,our method was employed to fabricate some key structures in one of the 16 Ch

关键词： electrons dynamics control femtosecond laser micro/nano fabrication pulse shaping

New determination of the gravitational constant G

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National Science Review 2020年第12期7卷 1796-1797页

作者： Zheng-tian lu Hefei National Laboratory for Physical Sciences at the Microscale CAS Center for Excellence in Quantum Information and Quantum PhysicsUniversity of Science and Technology of China

A new and so far the most precise determination of the gravitational constant G has been made by Jun luo and colleagues at the Huazhong University of Science and Technology(HUST) [1].The combination of G with two other most fundamental physical constants,the speed of light c and Planck’s constant h,can together construct the natural units(*** units) of time,length and *** relationships inform us that,

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解淀粉芽孢杆菌wxy-b3产脂肽类物质对苹果斑点落叶病菌的抑菌活性研究

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陕西科技大学学报 2025年第1期43卷 46-51页

作者：崔皓玥田露胡乐梅龚国利陕西科技大学食品科学与工程学院陕西西安710021

苹果斑点落叶病菌是苹果生产过程中常见的病害,严重危害苹果农作物的产量.脂肽作为一种主要来源于微生物次生代谢产物的抗菌剂,具有广谱抑菌、低耐药性、安全低毒等优势.本研究发现,解淀粉芽孢杆菌wxy-b3产生的脂肽类物质能够抑制苹果... 详细信息

苹果斑点落叶病菌是苹果生产过程中常见的病害,严重危害苹果农作物的产量.脂肽作为一种主要来源于微生物次生代谢产物的抗菌剂,具有广谱抑菌、低耐药性、安全低毒等优势.本研究发现,解淀粉芽孢杆菌wxy-b3产生的脂肽类物质能够抑制苹果斑点落叶病菌的生长,对苹果斑点落叶病菌的最小抑菌浓度为16 mg/mL,此浓度可造成该病原菌细胞膜电位超极化、线粒体膜电位去极化、大分子蛋白质和核酸泄露,以及胞内活性氧和丙二醛含量分别累积增加0.33倍和5.44倍,并通过扫描电镜进一步观察到MIC处理组的细胞形态起皱、细胞膜破损.上述结果表明,脂肽可能通过破坏细胞膜系统,导致细胞形态发生变化,细胞膜破裂,改变细胞膜通透性来达到抑菌效果.该研究将为脂肽作为抑制苹果斑点落叶病菌的抗菌剂奠定理论基础.

关键词：解淀粉芽孢杆菌脂肽类物质苹果斑点落叶病菌抑菌活性

Acoustomechanical constitutive theory for soft materials

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Acta Mechanica Sinica 2016年第5期32卷 828-840页

作者： Fengxian Xin tian Jian lu State Key Laboratory for Strength and Vibration of Mechanical Structures Xi’an Jiaotong University MOE Key Laboratory for Multifunctional Materials and Structures Xi’an Jiaotong University

Acoustic wave propagation from surrounding medium into a soft material can generate acoustic radiation stress due to acoustic momentum transfer inside the medium and material, as well as at the interface between the two. To analyze acoustic-induced deformation of soft materials, we establish an acoustomechanical constitutive theory by combining the acoustic radiation stress theory and the nonlinear elasticity theory for soft materials. The acoustic radiation stress tensor is formulated by time averaging the momentum equation of particle motion, which is then introduced into the nonlinear elasticity constitutive relation to construct the acoustomechanical constitutive theory for soft *** a specified case of soft material sheet subjected to two counter-propagating acoustic waves, we demonstrate the nonlinear large deformation of the soft material and analyze the interaction between acoustic waves and material deformation under the conditions of total reflection, acoustic transparency, and acoustic mismatch.

关键词： Acoustomechanical constitutive theory Acoustic radiation stress Soft material

Probabilistic Teleportation of the Three-Particle Entangled State via Entanglement Swapping

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Chinese Physics Letters 2001年第8期18卷 1004-1006页

作者： lu hong Laboratory of Quantum Communication and Quantum Computation University of Science and Technology of ChinaHefei 230026 Department of Physics Foshan UniversityFoshan 528000

A scheme of teleportation of a three-particle entangled state via entanglement swapping is *** is shown that if a two-particle entangled state and a three-particle entangled state(both are not maximum entangled states... 详细信息

关键词： particle state probabilistic

Photon Statistics of Photon-Added and Photon-Subtracted Two-Mode Squeezed Vacuum State

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Chinese Physics Letters 1999年第9期16卷 646-647页

作者： lu hong Department of Physics Foshan UniversityFoshan 528000

We introduce states a^(†m) |ξ〉and a^(m)|ξ〉, where |ξ〉is a two-mode squeezed vacuum state. We examine the properties of these states by analytic derivation. Because of the entanglement between the modes of the field, photon adding (or subtracting) for one of the modes results in interesting outcomes. For a two-mode squeezed vacuum state, the effect of annihilation of m a-photons is just the same as creation of m b-photons. Annihilation of photons of mode a (or mode b) actually increases the mean number of photons of mode a and mode b. We also calculate Mandel Q parameter, cross-correlation function and squeezing of the field.

关键词： field. state. vacuum

Bell’s palsy after inactivated COVID-19 vaccination in a patient with history of recurrent Bell’s palsy: A case report

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World Journal of Clinical Cases 2021年第27期9卷 8274-8279页

作者： Bin-Yan Yu lu-Sha Cen Ting Chen tian-hong Yang Department of Acupuncture and Moxibustion The First Affiliated Hospital of Zhejiang Chinese Medical University(Zhejiang Provincial Hospital of Traditional Chinese Medicine)Hangzhou 310006Zhejiang ProvinceChina Department of Ophthalmology The First Affiliated Hospital of Zhejiang Chinese Medical UniversityHangzhou 310006Zhejiang ProvinceChina Department of Ultrasonography The First Affiliated Hospital of Zhejiang Chinese Medical UniversityHangzhou 310006Zhejiang ProvinceChina Department of Acupuncture and Moxibustion The First Affiliated Hospital of Zhejiang Chinese Medical UniversityHangzhou 310006Zhejiang ProvinceChina

BACKGROUND With rapid and extensive administration of inactivated coronavirus disease 2019(COVID-19)vaccine to the general population in China,it is crucial for clinicians to recognize neurological complications or other side effects associated with COVID-19 *** SUMMARY Here we report the first case of Bell’s palsy after the first dose of inactivated COVID-19 vaccine in *** patient was a 36-year-old woman with a past history of Bell’s *** days after receiving the first dose of the Sinovac Life Sciences inactivated COVID-19 vaccine,the patient developed right-side Bell’s palsy and binoculus ***,artificial tears and fluorometholone eye drops were *** patient’s symptoms began to improve by day 7 and resolved by day *** As mRNA COVID-19 vaccine trials reported cases of Bell’s palsy as adverse events,we should pay attention to the occurrence of Bell’s palsy after inactivated COVID-19 vaccination.A history of Bell’s palsy,rapid increase of immunoglobulin M and immunoglobin G-specific antibodies to severe acute respiratory syndrome coronavirus 2 may be risk factors for Bell‘s palsy after COVID-19 vaccination.

关键词： Recurrent Bell’s palsy Keratoconjunctivitis Inactivated COVID-19 vaccination Case report