检索结果-南通市图书馆

Impact of Stable Protein-protein Interaction on Protein Conformational Space

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2015年第1期31卷 149-155页

作者： LI Wenzhao MENG Wei tian pu College of Life Science Key Laboratory of Molecular Enzymology and Engineering Ministry of Education Jilin University Changchun 130012 P. R. China

Backbone dihedral angle based clustering approach was applied to investigate the effect of protein complexation on backbone conformational space and the effect on protein dynamics. Three representative enzyme-inhibitor complexes and their comprised proteins were used as models for small- and moderate-sized globular proteins to compare available backbone conformational space before and after complexation. Microsecond time scale molecular dynamic simulations were generated to ensure sufficient statistics. The result suggests that stable protein-protein interactions lead to redistribution of protein backbone mobility and restriction of the protein backbone conformational space, especially for short time scale motions. Surprisingly, these effects are found to be uncorrelated with protein-protein interaction surface. Consistent with many experimental and computational observations, our results indicate that both induced-fit and conformational selection models play roles in stable protein complexation process, with the dominant role being different for different protein complexes.

关键词： Protein-protein interaction Conformational space Backbone dynamics Molecular dynamics simulation

PPAA:a parallel primitive assembly accelerator in graphics processor

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Journal of China Universities of Posts and Telecommunications 2020年第2期27卷 65-71页

作者： Deng Junyong Xie Xiaoyan Liu Yang tian pu School of Electronic Engineering Xi'an University of Posts and TelecommunicationsXi'an 710121China School of Computer Xi’an University of Posts and TelecommunicationsXi’an 710121China

Primitive assembly is an inevitable procedure of graphics rendering which performs the objects preparation for the following steps,however,the conventional approaches suffer from some issues,such as the missing of surface attribute,mismatch of color mode for clipped primitives,and performance bottleneck of rendering *** paper takes all these issues into considerations,and proposes a parallel primitive assembly accelerator(PPAA)which can solve not only the functional problems but also improve the shading *** register transfer level(RTL)circuit is designed and the detailed approach is *** prototype systems are implemented on Xilinx field programmable gate array(FPGA)XC6 VLX550 T and Altera FPGA EP2 C70 F896 *** experimental results show that PPAA can accomplish the assembly tasks correctly and with higher performance of 1.5x and 2.5x of two previous *** the most frequently independent primitives,the PPAA can efficiently enhance the throughput by squeezing out the pipeline bubbles and by balancing the pipeline stages.

关键词： primitive assembly parallel accelerator primitive characteristics graphics processor

Towards multi-party targeted model poisoning attacks against federated learning systems

在线全文

学校读者我要写书评

暂无评论

High-Confidence Computing 2021年第1期1卷 45-54页

作者： Zheyi Chen pu tian Weixian Liao Wei Yu Department of Computer and Information Sciences Towson UniversityMD 21252 USA

The federated learning framework builds a deep learning model collaboratively by a group of connected devices via only sharing local parameter updates to the central parameter ***,the lack of transparency in the local data resource makes it prone to adversarial federated attacks,which have shown increasing ability to reduce learning *** research efforts either focus on the single-party attack with impractical perfect knowledge setting and limited stealthy ability or the random attack that has no control on attack *** this paper,we investigate a new multi-party adversarial attack with the imperfect knowledge of the target *** by an adversary,a number of compromised devices collaboratively launch targeted model poisoning attacks,intending to misclassify the targeted samples while maintaining stealthy under different de-tection ***,the compromised devices jointly minimize the loss function of model training in different *** overcome the update scaling problem,we develop a new boosting strategy by introducing two stealthy *** experimental results,we show that under both perfect knowledge and limited knowl-edge settings,the multi-party attack is capable of successfully evading detection strategies while guaranteeing the *** also demonstrate that the learned model achieves the high accuracy on the targeted samples,which confirms the significant impact of the multi-party attack on federated learning systems.

关键词： Adversarial federated learning Perfect knowledge Limited knowledge Boosting strategy High-confidence computing

Edge computing-Based mobile object tracking in internet of things

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

High-Confidence Computing 2022年第1期2卷 19-27页

作者： Yalong Wu pu tian Yuwei Cao Linqiang Ge Wei Yu Department of Computer Science and Engineering North Central CollegeNapervilleUSA Department of Computer and Information Sciences Towson UniversityTowsonUSA Department of Computer Science University of Illinois at ChicagoChicagoUSA School of Computer Science Columbus State UniversityColumbusUSA

Mobile object tracking,which has broad applications,utilizes a large number of Internet of Things(IoT)devices to identify,record,and share the trajectory information of physical ***,IoT devices are energy con-strained and not feasible for deploying advanced tracking techniques due to significant computing *** address these issues,in this paper,we develop an edge computing-based multivariate time series(EC-MTS)framework to accurately track mobile objects and exploit edge computing to offload its intensive computation ***,EC-MTS leverages statistical technique(i.e.,vector auto regression(VAR))to conduct arbitrary historical object trajectory data revisit and fit a best-effort trajectory model for accurate mobile object location *** framework offers the benefit of offloading computation intensive tasks from IoT devices by using edge computing *** have validated the efficacy of EC-MTS and our experimental results demon-strate that EC-MTS framework could significantly improve mobile object tracking efficacy in terms of trajectory goodness-of-fit and location prediction accuracy of mobile *** addition,we extend our proposed EC-MTS framework to conduct multiple objects tracking in IoT systems.

关键词： Internet of things Edge computing Architecture Mobile object tracking Vector auto regression

Detection of QTLs with Additive Effects, Epistatic Effects, and QTL × Environment Interactions for Zeleny Sedimentation Value Using a Doubled Haploid Population in Cultivated Wheat

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2009年第9期8卷 1039-1045页

作者： ZHAO Liang LIU Bin ZHANG Kun-pu tian Ji-chun DENG Zhi-ying State Key Laboratory of Crop Biology Group of Quality Wheat Breeding Shandong Agricultural University Tai＇an 271018 P.R.China

In order to understand the genetic basis for Zeleny sedimentation value （ZSV） of wheat, a doubled haploid （DH） population Huapei 3 × Yumai 57 （Yumai 57 is superior to Huapei 3 for ZSV）, and a linkage map consisting of 323 marker loci were used to search QTLs for ZSV. This program was based on mixed linear models and allowed simultaneous mapping of additive effect QTLs, epistatic QTLs, and QTL x environment interactions （QEs）. The DH population and the parents were evaluated for ZSV in three field trials. Mapping analysis produced a total of 8 QTLs and 2 QEs for ZSV with a single QTL explaining 0.64-14.39% of phenotypic variations. Four additive QTLs, 4 pairs of epistatic QTLs, and two QEs collectively explained 46.11% of the phenotypic variation （PVE）. This study provided a precise location of ZSV gene within the Xwmc 93 and GluD1 interval, which was designated as Qzsv-1D. The information obtained in this study should be useful for manipulating the QTLs for ZSV by marker assisted selection （MAS） in wheat breeding programs.

关键词： doubled haploid population Zeleny sedimentation value quantitative trait loci (QTLs) wheat (Triticum aestivum L.)

Functional analysis of the orphan genes Tssor-3 and Tssor-4 in male Plutella xylostella

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2021年第7期20卷 1880-1888页

作者： LI tian-pu ZHANG Li-wen LI Ya-qing YOU Min-sheng ZHAO Qian State Key Laboratory of Ecological Pest Control for Fujian-Taiwan Crops Institute of Applied EcologyFujian Agriculture and Forestry UniversityFuzhou 350002P.R.China Joint International Research Laboratory of Ecological Pest Control Ministry of EducationFuzhou 350002P.R.China Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops Ministry of Agriculture and Rural AffairsFuzhou 350002P.R.China

Orphan genes are genes with no sequence homologues in other species. Here, we identified two orphan genes, namely, Tssor-3 and Tssor-4, in Plutella xylostella. Both genes contained a signal peptide sequence, suggesting their functions as secreted proteins. Expression pattern analysis based on real-time quantitative PCR(qPCR) showed that both orphan genes were specifically expressed in all male gonads except the testes. The expression of both the orphan genes peaked at the male adult stage. Immunofluorescence assays suggested that the two proteins were seminal proteins, indicating their potential roles in male reproductive regulation. To further explain their functions, we knocked down the expression of these two genes by RNA interference(RNAi). The results showed that the expression of Tssor-3 and Tssor-4 was significantly downregulated at 24 h after injection compared to that of the controls. Biological assays showed that the number of laid eggs and the hatching rate of offspring eggs were significantly reduced when the expression of Tssor-3 and Tssor-4 was reduced, suggesting that the two orphan genes played a role in male fertility in P. xylostella. Our results provide evidence that orphan genes are involved in male reproductive regulation, which is important for male fitness during evolution.

关键词： Plutella xylostella orphan genes male fertility RNA interference

Infusion of donor hepatic non-parenchymal cells prolongs survival of skin allografts in mice:role of microchimerism and IL-4

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2007年第1期6卷 34-37页

作者： Ding, Xiao-Ming Xue, Wu-Jun Ji, Zong-Zheng tian, pu-Xun Xian Jiaotong Univ Affiliated Hosp 1 Sch Med Dept Renal Transplantat Xian 710061 Peoples R China

BACKGROUND: In the mouse skin allograft model, specific immune tolerance to the donor was induced by injection of donor hepatic non-parenchymal cells (NPCs). This markedly prolonged the survival time of the allograft. The mechanism of the induction of immune tolerance with donor hepatic NPCs is thought to be related to microchimerism and the IL-4 level. This work aimed at exploring the way of inducing immune tolerance and understanding the mechanism. METHODS: C57BL/6 (B6) mice were primed by intravenous injection of 2 X10(7) NPCs from C3H mice. Cyclophosphamide (200 mg/kg) was injected intraperitoneally 48 hours later. Eighteen days after the NPC injection, skin from C3H mice was transplanted to B6 mice and the survival of the grafts was assessed. The immune reaction of splenocytes from the treated B6 mice to donor-specific T-cells was measured by H-3-TdR incorporation. Microchimerism in the spleen was determined by flow cytometric analysis sytem (FCAS) analysis, and the serum level of IL-4 was assayed by ELISA at designed times. RESULTS: The survival time of the skin graft was markedly prolonged from 10 days to 70 days in controls. Microchimerism. in the spleen was found as early as day I post-NPC injection, then it increased steadily, and there was a positive relationship between graft survival and the quantity of microchimerism. The ELISA results showed that NPC infusion enhanced IL-4 production, especially in the mice with longer graft survival. CONCLUSION: Donor NPC infusion pre-transplant can prolong the survival of the skin graft and microchimerism and high levels of IL-4 may be involved.

关键词： hepatic non-parenchymal cell immune tolerance skin allograft

Co-expression of sCD40LIg and CTLA4Ig mediated by adenovirus prolonged mouse skin allograft survival

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2006年第6期7卷 436-444页

作者： LI Zhao-lun tian pu-xun XUE Wu-jun WU Jun Department of Renal Transplantation the First Affiliated Hospital School of Medicine Xi＇an Jiaotong University Xi＇an 710061 China Institute of Burn Research Southwest Hospital the Third Military Medical University Chongqing 400038 China

Objective： To investigate the role of simultaneous blockade of CD40/CD40L and B7/CD28 pathways in the immune tolerance via co-expression of sCD40LIg and CTLA4Ig mediated by replication-defective adenovirus. Methods： Ad-sCD40LIg- IRES2-CTLA4Ig, replication-defective adenovirus co-expressing sCD40LIg and CTLA4Ig, was constructed and identified. The co-expression of sCD40LIg and CTLA4Ig was evaluated with confocal laser scanning microscope and Western blotting. Skin transplantations of C57BL/6 to BALB/c mice were performed. PBS, Ad-Shuttle-CMV and Ad-sCD40LIg-IRES2-CTLA4Ig were administered. Skin graft survival was monitored and the mRNA expression of both genes was evaluated in the skin allografts. Results： Ad-sCD40LIg-IRESE-CTLA4Ig was constructed successfully and identified. The co-expression of sCD40LIg and CTLA4Ig was identified with confocal laser scanning microscopy and Western blotting. Compared to the skin graft mean survival time （MST） of non-treated group （（5.75±0.71） d） or Ad-Shuttle-CMV-treated group （（5.50±0.53） d）, the skin graft MST was dramatically prolonged in the Ad-sCD40LIg-IRESE-CTLA4Ig-treated group （（ 16.38± 1.19） d, P〈0.001）. The mRNA expression of both genes was detected. Conclusion： Ad-sCD40LIg-IRES2-CTLA4Ig, a replication-defective adenovirus carrying genes encoding sCD40LIg and CTLA4Ig, was constructed. Simultaneous blockade of CD40/CD40L and B7/CD28 costimulatory pathway mediated by replication-defective adenovirus significantly prolonged skin allografi survival in mice.

关键词： Immune tolerance CTLA4Ig sCD40LIg Replication-defective recombinant adenovirus Skin graft

Sulfonylcalix[4]arene-based Coordination Supercontainers

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Structural Chemistry 2020年第12期39卷 2077-2084,2053页

作者： HE Can SHENG tian-pu DAI Feng-Rong CHEN Zhong-Ning College of Chemistry and Materials Science Fujian Normal UniversityFuzhou 350007China State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002China

Sulfonylcalix[4]arenes-based coordination containers,namely metal-organic supercontainers(MOSCs),are a new class of coordination containers constructed from the self-assembly of divalent metal ions,suitable carboxylate linkers,and sulfonylcalix[4]arenes container *** feature both endo cavity surrounded by carboxylate linkers and exo cavities originated from the upper rim of sulfonylcalix[4]*** molecular topologies and endo cavity of MOSCs are tuneable via judicious design of carboxylate linkers,while the modulation of endo cavity are accessible by chemical modification on the para substituent group of the sulfonylcalix[4]*** this paper,recent advances and typical examples of design and functionalization of MOSCs are presented.

关键词： coordination container sulfonylcalixarene metal-organic supercontainer self-assembly