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Genome-Wide Identification and Characterization of the <i>Dof</i>Transcription Factor Gene Family in <i>Phaseolus vulgaris</i>L.

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American Journal of Plant Sciences 2017年第12期8卷 3233-3257页

作者： Tania Mayumi Ito Claudia Borsari Trevizan tiago benedito dos santos Silvia Graciele Hülse de Souza Programa de Pós-Graduacao em Biotecnologia Aplicada à Agricultura Laboratório de Biologia Molecular Universidade Paranaense (UNIPAR) Umuarama Brazil Programa de Pós-Graduacao em Agronomia Universidade do Oeste Paulista (UNOESTE) Presidente Prudente Brazil

The Dof (DNA-binding with one finger) proteins are a class of plant-specific transcription factors that can trigger several processes involved in plant growth and development, as well as in stress responses. Here, we performed a systematic bioinformatics analysis to characterize all Dof genes in common bean, which included analysis of the genome sequence, conserved protein domains, chromosomal locations, subcellular locations, phylogenetic relationships, gene duplications, and gene expression profiles in different tissues. Bioinformatics analysis revealed 36 putative genes related to PvDof that were classified into seven subfamilies (A, B1, B2, C1, C2, D1, and, D2) by comparative phylogenetic analysis. Based on our genome duplication analysis, a total of 36 genes were found to be distributed on all 11 chromosomes, and they expanded through gene duplication in tandem, suggesting the involvement of segmental duplication events in the evolutionary process. Synteny events and phylogenetic comparisons of the Dof proteins of common bean with those of A. thaliana, O. sativa, and G. max L. led to the identification of several orthologous and paralogous genes, which provided further insight into the diversity of the evolutionary characteristics of genes of this family in other plant species. Expression profiles revealed that most of the PvDof genes were expressed in different tissues, indicating that PvDof genes may be involved in various physiological functions during plant development. The results of this study provide additional information and potential biotechnological resources for further understanding the molecular basis of this gene family and consequently improvement of common bean crops.

关键词： Common Bean DNA-Binding with One Finger (Dof) Domain Proteins Tran-scription Factor

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In Silico and in Vitro Analysis of Pyrazolone Derivatives against Zika Virus and Identification of Potential NS5 Methyltransferase Inhibitors by Molecular Docking

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Journal of Biosciences and Medicines 2024年第7期12卷 214-229页

作者： Luciene Soares Silva tiago Soares Barbosa Maria Leonisa Sanchez-Nuñez Aldenise Mont Serrat Rosa da Silva Vitor Won-Held Rabelo Leonardo dos santos Corrêa Amorim Leonardo Alves Miceli Percilene Fazolin Vegi Alice Bernardino Helena Carla Castro Izabel Christina Nunes de Palmer Paixão Science and Biotechnology Graduate Program Department of Cellular and Molecular Biology Institute of Biology Fluminense Federal University Niteri Brazil Science and Biotechnology Graduate Program Department of Cellular and Molecular Biology Institute of Biology Fluminense Federal University Niterói Brazil Laboratory of Antibiotics Biochemistry Teaching and Molecular Modeling Institute of Biology Fluminense Federal University Niterói Brazil Laboratory of Organic Chemistry at the Institute of Chemistry Institute of Biology Fluminense Federal University Niterói Brazil

Zika virus (ZIKV), a mosquito-borne flavivirus, has been associated with benign infections for decades. However, it has become a public health concern due to its association with severe fetal and neurological complications. Although many efforts have been made to control ZIKV infection, approved vaccines or antiviral drugs are still lacking. Consequently, the development of new effective anti-ZIKV agents is urgently needed. In this context, we investigated the antiviral potential of pyrazolone derivatives against ZIKV replication using in silico and in vitro methods. The four pyrazolone derivatives evaluated (1a, 1b, 1c, and 1d) inhibited over 50% of ZIKV replication with low cytotoxicity. Among them, compound 1b exhibited the most potent activity (EC50 = 4.3 μM) and the highest selectivity (SI = 342). Mechanism of action studies indicated that these compounds act at early stages of virus replication, and compound 1b can also directly inactivate ZIKV particles. Molecular docking studies suggested that these compounds can bind to and block the activity of ZIKV NS5 methyltransferase. Finally, pharmacokinetic and toxicological predictions have reinforced the safety and drug-like profiles of these derivatives. In conclusion, the pyrazolone scaffold proved to be valuable for anti-ZIKV drug development, and the derivatives studied deserve further investigation.

关键词： Zika Pyrazolone NS5 Methyltransferase Antivirals

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Changes on Anuran Tadpole Functional Diversity along an Environmental Gradient at the Southernmost Atlantic Rainforest Remnant

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Asian Herpetological Research 2020年第1期11卷 63-70页

作者： Victor Mendes LIPINSKI Andre Passaglia SCHUCH tiago Gomes dos santos Post Graduation Program of Animal Biodiversity Federal University of Santa MariaLaboratory of HerpetologyDepartment of BiologyAv.Roraima no 1000 building 17room 1140Camobi97105-900Santa MariaRSBrazil Universidade Federal do Pampa Av.Antonio Trilha1847Centro973000000 Sao GabrielRSBrasil Southern Regional Space Research Center CRS/INPE-MCTIP.O.Box 5021room 104197110-970Santa MariaRSBrazil

For decades the ecologists are using the same methods in order to determine the environmental health of a community,however,the crescent need on defining how these processes act,triggered the development of new methods that could classify the organisms and their function at the *** we used the anuran larvae to understand the effects of an environmental gradient on the structure and organization of the anuran assemblages along it,and to access in a realistic form,the actual state of preservation of the la rgest remnant of Atlantic Rainforest on the Southernmost portion of *** found that despite the higher diversity of species and the conservation unit status of the place,the ecosystem itself is still in danger,given the low number of functional groups and thus a higher number of ecosystem services that can be lost.

关键词： Anuran ecosystem function conservation unities tadpoles

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Quantitative hazard assessment system(Has-Q) for open pit mine slopes

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International Journal of Mining Science and Technology 2019年第3期29卷 419-427页

作者： Tatiana Barreto dos santos Milene Sabino Lana tiago Martins Pereira Ismet Canbulat Mining Engineering Department Universidade Estadual de Minas GeraisJoao Monlevade 35930314Brazil Mining Engineering Department Universidade Federal de Ouro PretoOuro Preto 35400000Brazil Statistical Department Universidade Federal de Ouro PretoOuro Preto 35400000Brazil School of Minerals and Energy Resources Engineering University of New South WalesSydneyNSW 2052Australia

Rock slope hazard assessment is an important part of risk analysis for open pit *** main parameters that can lead to rock slope failures are the parameters traditionally used in geomechanical classifications,the slope geometrical parameters and external factors like rainfall and *** paper presents a methodology for a hazard assessment system for open pit mine slopes based on 88 cases collated around the world using principal components analysis,discriminant analysis and confidence *** historical cases used in this study included copper,gold,iron,diamond,lead and zinc,platinum and claystone *** variables used in the assessment methodology are uniaxial compressive strength of intact rock;spacing,persistence,opening,roughness,infilling and orientation of the main discontinuity set;weathering of the rock mass;groundwater;blasting method;and height and inclination of the *** principal component analysis was used to quantify the data,the discriminant analysis was used to establish a rule to classify new slopes about its stability *** provide a practical hazard assessment system,confidence ellipses were used to propose a hazard graph and generate the *** discriminant rule developed in this research has a high discrimination capacity with an error rate of 11.36%.

关键词： Hazard assessment Risk analysis Open pit mine slopes Multivariate statistics

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Evaluation of xylanase and a fermentable xylo-oligosaccharide on performance and ileal digestibility of broiler chickens fed energy and amino acid deficient diets

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Animal Nutrition 2021年第2期7卷 488-495页

作者： Gemma González-Ortiz tiago T.dos santos Michael R.Bedford AB Vista 3Woodstock CourtMarlborough Business ParkMarlboroughWiltshireSN84ANUK

The objective of this study was to evaluate the effect of a product consisting of a combination of xylanase and xylo-oligosaccharide(STBIO)on performance and ileal digestibility of broiler chickens fed energy and amino acid(AA)deficient ***-old male Ross 308 broiler chicks were randomly allocated to 8 pens per treatment,with 25 chicks per *** based on wheat-corn-soybean meal diets were arranged in a 3×2 factorial design:a positive control that met or exceeded nutrient recommendations(PC),a negative control diet with a 50 kcal/kg apparent metabolizable energy(AME)reduction(NC1)and NC1 with a 3%reduction in AA content(NC2),each with or without supplementation of 100 g/t of the *** weight gain(BWG),feed intake,feed conversion ratio corrected for mortality(FCR)and the European production efficiency factor(EPEF)were recorded from 0 to 42 *** d 42,ileal samples were collected to determine dry matter(DM),organic matter(OM),ash,protein and energy digestibility.A significant interaction was observed for BWG and feed intake(P<0.001).The energy and AA reduction reduced(P<0.05)BWG when compared to the *** effect of STBIO on BWG was greater in NC1(+451 g/bird)than in NC2(+314 g/bird)or PC(+176 g/bird)diets(P<0.05),and that in NC2 with STBIO was equal to that in PC without STBIO,and that in NC1 with STBIO was equal to that in PC with *** interactions were observed on the EPEF or FCR;however,STBIO improved EPEF(P<0.001)and FCR(P<0.001)irrespective of the energy reduction or AA *** intake of digestible DM,OM,ash and energy for the finisher period was increased with STBIO supplementation(P<0.01).A significant interaction was observed for the intake of digestible ***1 and NC2 reduced the intake of digestible protein;however,when STBIO was supplemented,it was improved in both diets to similar levels to the *** stimbiotic supplementation improved performance of broiler chickens fed all diets,particularly those deficient in AME and AA.

关键词： Xylanase Fermentable oligosaccharide Broiler Stimbiotic

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Genetic diversity and structure of Drimys brasiliensis in southern Brazil:insights for conservation

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Journal of Forestry Research 2020年第4期31卷 1325-1332页

作者： Alexandre Mariot tiago Montagna Maurício Sedrez dos Reis Núcleo de Pesquisas Em Florestas Tropicais Pós-Graduacāo Em Recursos Genéticos VegetaisCentro de Ciências AgráriasUniversidade Federal de Santa CatarinaRodovia Admar Gonzaga1346FlorianópolisSC 88034-000Brazil

Population genetics studies are widely recognized for generating useful knowledge for biodiversity *** date,however,little is known about the levels and distribution of genetic diversity of Drymis brasiliensis(Miers LC),a tree species from the Atlantic ***,in this study,we investigated how genetic diversity is distributed within and among populations of *** from southern Brazil using allozyme markers to genotype reproductive trees(8 populations)and seedlings(3 populations).Furthermore,in two populations,we established two permanent plots(5.1 and 1 ha)to analyze fine-scale genetic structure(FSGS).Studied populations presented low levels of genetic diversity(reproductive=0.085;seedlings=0.054)and high fixation indexes(reproductive=0.396;seedlings=0.231).Genetic divergence among populations was equal to 0.05,which is significant,signaling that few populations can conserve large portions of the species total genetic *** was only detected for one population,when reproductive individuals were separated by less than40 *** genetic diversity combined with high fixation indexes clearly signal a risk of losing ***,conservation efforts should be aimed at enhancing gene flow within the studied populations.

关键词： Atlantic Rainforest Effective population size Fine-scale genetic structure Seed collection

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Experimental evidence of pressure effects on spinel dissolution and peridotite serpentinization kinetics under shallow hydrothermal conditions

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Geoscience Frontiers 2024年第2期15卷 236-250页

作者： Yuri de Melo Portella Rommulo Vieira Conceição tiago Abreu Siqueira Lucas Bonan Gomes Rodrigo Sebastian Iglesias Programa de Pós-Graduação em Geociências(PPGGEO) Universidade Federal do Rio Grande do SulPorto AlegreBrazil Petrobras–Petróleo Brasileiro S.A. Avenida Henrique Valadares 28CentroRio de Janeiro(RJ)20231-030Brazil Instituto do Petróleo e dos Recursos Naturais-PUCRS–Pontifícia Universidade Católica do Rio Grande do Sul 90619-900Porto AlegreBrazil Universidade Federal do Rio Grande do Sul Instituto de GeociênciasLaboratório de Difratometria de Raios XPorto AlegreBrazil Escola Politécnica–PUCRS-Pontifícia Universidade Católica do Rio Grande do Sul 90619-900 Porto AlegreBrazil

Serpentinization reactions are paramount to understand hydro-geothermal activity near plate boundaries and mafic–ultramafic massifs,as well as fluid and element transfer between the Earth’s mantle and ***,fluid-rock element exchange and serpentinization kinetics under shallow hydrothermal conditions is still largely *** we present two constant temperature(230℃)time-series of natural peridotite(77.5%olivine;13.7%enstatite;6.8%diopside;2%spinel)serpentinization experiments:at 13.4 MPa;and 20.7 ***-enriched lizardite was the main secondary mineral in all runs after olivine(olv)and orthopyroxene(opx)serpentinization(without any detectable brucite,talc or magnetite),while primary spinel and diopside partially dissolved during the *** serpentinization stages comprises intrinsically coupled reactions between olivine and enstatite,as Al and Si are progressively transferred from orthopyroxene-derived to olivine-derived serpentine,while the opposite is true for Mg and Fe,with homogenization of serpentines compositions after 40 *** Ni/Cr ratios of serpentines,however,remain diagnostic of the respective primary *** average serpentine content indicates fast serpentinization rates of 0.55 wt.%·day^(-1)(0.26 mmol·day^(-1))and 0.26 wt.%·day^(-1)(0.13 mmol·day^(-1))at 13.4 and 20.7 MPa,*** 2x faster serpentinization kinetics at lower pressure is likely linked to enhanced spinel dissolution leading to one order of magnitude higher available Al,which accelerates olivine serpentinization while delays orthopyroxene ***,time-dependent increase in solid products masses suggests rock volume expands linearly 0.37%±0.01%per serpentine wt.%independently of *** balance constrains suggests olv:opx react at~5:2 and~3:2 M ratios,resulting in Si-deficient and Si-saturated serpentines at the end of the low-pressure series(13.4 MPa)and high-pressure series(20.7 MPa),***

关键词： Serpentine Volume expansion Serpentinization fluids Lost City hydrothermal field Serpentinite-hosted hydrothermal systems Hydrogen(H_(2))generation

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Electromagnetic Characterization of Recyclable Polymer Nanofibers Based on PSU/Carbonyl Iron

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Journal of Renewable Materials 2019年第3期7卷 279-287页

作者： Daniel Consoli Silveira tiago Teixeira da Silva Braga Daniel Molina Gil Newton Adriano dos santos Gomes Lilia Müller Guerrini Edson Cocchieri Botelho Department of Materials and Technology Sao Paulo State University(UNESP)School of EngineeringGuaratinguetá/SP-Brazil Electronic Warfare Laboratory Technological Institute of Aeronautics(ITA)Sao Josédos Campos/SP-Brazil Institute of Science and Technology Federal University of Sao Paulo(UNIFESP)Sao Josédos Campos/SP-Brazil

This study investigated and defined the optimal processing parameters for the electrospinning of polysulfone polymer solutions with N,*** of parameters such as solute concentration,electrical voltage,and working distance were correlated with the quality of the obtained nanofibers using morphological characterization via scanning electron microscopy(SEM).Carbonyl iron additive was dispersed in the polymer solutions,using ultrasonic tip,and the material processed via electrospinning with aforementioned parameters *** with the property of interaction with electromagnetic waves were *** dispersion of different concentrations of the additive and electromagnetic characterizations in the X-band of microwaves(8.2 and 12.4 GHz),using vector network analyzer(VNA)and rectangular waveguide,allowed the identification of the materials electromagnetic *** parameters allowed the calculation of reflected and transmitted energy by the material.

关键词： Carbonyl iron electromagnetic characterization electrospinning nanofibers polysulfone

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Xylanase, protease and superdosing phytase interactions in broiler performance, carcass yield and digesta transit time

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Animal Nutrition 2017年第2期3卷 121-126页

作者： tiago T.dos santos Helen V.Masey O'Neill Gemma González-Ortiz Daniel Camacho-Fernández Carlos López-Coello AB Vista Facultad de Medicina Veteriaria y Zootecnia Universidad Nacional Autónoma de México

The interaction of xylanase, protease and superdosing(1,500 FTU/kg) phytase in a 2×2×2 factorial arrangement was studied in broilers fed sorghum-based diets. A total of 2,800 one-day-old unsexed Ross308 chicks were housed in 56 pens with 50 birds per pen, with or without inclusion of xylanase, protease and phytase, totaling 8 treatments and 7 replicates per treatment. Body weight(BW) and feed intake(FI)were measured at 21 and 42 days of age, and mortality corrected feed conversion ratio(FCR) was calculated for each period and cumulatively. Tibia ash and carcass yield were determined in 2 birds per replicate at 21 and 42 days of age, respectively. Digesta transit time was determined at 21, 28, 35 and 42 days of age using 5 birds per replicate. Results showed that superdosing phytase increased BW and FI at42 days of age(P dosing phytase increased transit time when included in a diet containing xylanase, and no change with protease inclusion. In conclusion, the beneficial effects of xylanase, protease and superdosing phytase in broiler performance were not additive. This limitation is likely not related to the lack of efficacy of any one of the individual enzymes but to a limitation of the bird to respond additively to successive additions of enzymes.

关键词： Sorghum Tibia ash Sub-additivity Exogenous enzyme

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Synthesis and structure of a praseodymium (Ⅲ) complex with carboxylate ligand：A thermal and spectroscopic study

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Journal of Rare Earths 2018年第10期36卷 1090-1097页

作者： Kátia Veronica Tenório José Augusto Teixeira Leandro Moreira de Campos Pinto Flávio Júnior Caires Oswaldo Treu-Filho Fáhio Alencar dos santos tiago André Denck Colman Alexandre Cuin Cláudio Teodoro de Carvalho Federal University of Grande Dourados CP 364 79804-970 Dourados MS Brazil Institute of Chemistry State University of Sao Paulo 14.801-970 Araraquara SP Brazil Chemistry Institute Federal University of Mato Grosso do Sul 79074-460 Campo Grande MS Brazil Chemistry Department State University of Sao Paulo 17033-360 Bauru SR Brazil Federal University of Juiz de Fore 36036-330 Juiz de Fora MG Brazil

One Pr（Ⅲ） lanthanide ion complex was initially synthesized and characterized by TGA-DSC in air atmosphere, as well as characterized by CHN elemental analysis, defining the stoichiometric ratio as Pr（DMBz）3. The gaseous products evolved during the thermal decomposition were also monitored in N2 atmosphere employing TGA/FT-IR system. A crystal structure is obtained by state-of-the-art powder X-rays diffraction methods measured in conventional laboratory equipment and refined by the Rietveld method, which defined it as a monoclinic system of the space group P21/C with a polymeric crystal structure, [Pr（DMBZ）3]n. FT-IR theoretical spectrum and time-dependent density functional theory（TD-DFT） were calculated from TGA-DSC and crystalline system data. The experimental and theoretical FT-IR spectra present a high correlation degree when the main stretching bands are compared, while the energy transfer（HOMO - LUMO） in their neighborhoods suggests the main contributions of the light-emitting states.

关键词： Complex Thermal analysis Powder diffraction state-of-art Polymeric structure Theoretical calculation Rare earths

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