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HBP1 inhibits chicken preadipocyte differentiation by activating the STAT3 signaling via directly enhancing JAK2 expression

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Journal of Integrative Agriculture 2022年第6期21卷 1740-1754页

作者： CHEN Hong-yan CHENG Bo-han ma Yan-yan ZHANG Qi LENG Li WANG shou-zhi LI Hui Key Laboratory of Chicken Genetics and Breeding Ministry of Agriculture and Rural AffairsHarbin 150030P.R.China Key Laboratory of Animal Genetics Breeding and ReproductionEducation Department of Heilongjiang ProvinceHarbin 150030P.R.China College of Animal Science and Technology Northeast Agricultural UniversityHarbin 150030P.R.China

Obesity presents a serious threat to human health and broiler *** expansion of adipose tissue is mainly regulated by the differentiation of *** differentiation of preadipocytes is a complex biological process regulated by a variety of transcription factors and signaling *** studies have shown that the transcription factor HMG-box protein 1(HBP1)can regulate the differentiation of mouse 3T3-L1 preadipocytes by activating the Wnt/β-catenin signaling ***,it is unclear whether HBP1 involved in chicken preadipocyte differentiation and which signaling pathways it *** aim of the current study was to explore the biological function and molecular regulatory mechanism of HBP1 in the differentiation of chicken *** expression patterns of chicken HBP1 in abdominal adipose tissue and during preadipocyte differentiation were analyzed by RT-qPCR and Western *** preadipocyte stably overexpressing HBP1 or knockout HBP1 and their control cell line were used to analyze the effect of HBP1 on preadipocyte differentiation by oil red O staining,RT-qPCR and Western *** 45-Pathway Reporter Array was used to screen the signal pathways that HBP1 regulates in the differentiation of chicken *** inhibitor and siRNA for signal transducer and activator of transcription 3(STAT3)were used to analyze the effect of STAT3 on preadipocyte *** preadipocyte stably overexpressing HBP1 was transfected by the siRNA of STAT3 or treated with a chemical inhibitor of STAT3 for the rescue *** results of gene expression analysis showed that the expression of HBP1 was related to abdominal fat deposition and preadipocyte differentiation in *** results of function gain and loss experiments indicated that overexpression/knockout of HBP1 in chicken preadipocytes could inhibit/promote(P<0.05)lipid droplet deposition and the expression of adipogenesis-related ***,HB

关键词： chicken HBP1 preadipocyte differentiation STAT3 signaling pathway

Transforming Multidisciplinary Customer Requirements to Product Design Specifications

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Chinese Journal of Mechanical Engineering 2017年第5期30卷 1069-1080页

作者： Xiao-Jie ma Guo-Fu Ding Sheng-Feng Qin Rong Li Kai-Yin Yan shou-Ne Xiao Guang-Wu Yang Institute of Advanced Design and manufacturing Southwest Jiaotong University School of Design Northumbria University State Key Laboratory of Traction Power Southwest Jiaotong University

With the increasing of complexity of complex mechatronic products, it is necessary to involve multidis- ciplinary design teams, thus, the traditional customer requirements modeling for a single discipline team becomes difficult to be applied in a multidisciplinary team and project since team members with various disciplinary backgrounds may have different interpretations of the customers＇ requirements. A new synthesized multidisci- plinary customer requirements modeling method is pro- vided for obtaining and describing the common understanding of customer requirements （CRs） and more importantly transferring them into a detailed and accurate product design specifications （PDS） to interact with dif- ferent team members effectively. A case study of designing a high speed train verifies the rationality and feasibility of the proposed multidisciplinary requirement modeling method for complex mechatronic product development. This proposed research offersthe instruction to realize the customer-driven personalized customization of complex mechatronic product.

关键词： Complex mechatronic product Crowdsourcing - Multidisciplinary customer requirementsmodeling Disciplinary specialty language dictionary High-speed train

Residue management induced changes in soil organic carbon and total nitrogen under different tillage practices in the North China Plain

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Journal of Integrative Agriculture 2019年第6期18卷 1337-1347页

作者： PU Chao KAN Zheng-rong LIU Peng ma shou-tian QI Jian-ying ZHAO Xin ZHANG Hai-lin College of Agronomy and Biotechnology China Agricultural University/Key Laboratory of Farming SystemMinistry of Agriculture

Crop residue retention has been considered a practicable strategy to improve soil organic carbon(SOC)and total nitrogen(TN),but the effectiveness of residue retention might be different under varied tillage *** evaluate the effects of residue management on the distribution and stocks of SOC and TN under different tillage practices,a bifactorial experiment with three levels for tillage practices(no-tillage,rotary tillage,and conventional tillage)and two levels for residue managements(residue retention and residue removal)was conducted in the North China Plain(NCP).Results showed that after a short experimental duration(3–4 years),concentrations of SOC and TN in the 0–10 cm layer were higher under no-tillage than under conventional tillage,no matter whether crop residues were retained or *** retention increased SOC and TN concentrations in the upper layers of soil to some degree for all tillage practices,as compared with residue removal,with the greatest increment of SOC concentration occurred in the 0–10 cm layer under rotary tillage,but in the 10–30 cm layer under conventional *** stocks of SOC in the 0–50 cm depth increased from 49.89 Mg ha–1 with residue removal to 53.03 Mg ha–1 with residue ***,no-tillage did not increase SOC stock to a depth of 50 cm relative to conventional tillage,and increased only by 5.35%as compared with rotary ***,residue retention may contribute more towards SOC sequestration than ***,the combination between residue retention and no-tillage has the greatest advantage in enhancing SOC and TN in the NCP region.

关键词： no-tillage residue retention residue removal stratification ratio SOC stock total nitrogen stock

Outcomes of cardiac surgery in senior aged patients with ventricular dysfunction:analysis of a large national database

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Journal of Geriatric Cardiology 2021年第1期18卷 1-9页

作者： Han-Wei TANG Kai CHEN Jian-Feng HOU Xiao-Hong HUANG Sheng LIU Han-Ping ma Sheng-shou HU Department of Cardiovascular Surgery National Center for Cardiovascular DiseasesState Key Laboratory of Cardiovascular DiseaseFuwai HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina Department of Special Medical Treatment Center Fuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Science and Peking Union Medical CollegeBeijingChina

OBJECTIVE In patients undergoing cardiac surgery,reduced preoperative ejection fraction(EF)and senior age are associated with a worse *** most outcome data available for these patients are mainly from Western surgical populations involving specific surgery types,our aim is to evaluate the real-world characteristics and perioperative outcomes of surgery in senior-aged heart failure patients with reduced EF across a broad range cardiac *** Data were obtained from the China Heart Failure Surgery Registry(China-HFSR)database,a nationwide multicenter registry study in China's *** variable regression analysis was performed in patients over 75 years old to identify risk factors associated with *** From 2012 to 2017,578 senior-aged(>75 years)patients were enrolled in China HFSR,21.1%of whom were *** coronary bypass grafting(CABG)were performed in 71.6%of patients,10.1%of patients underwent isolated valve surgery and 8.7%received CABG combined with valve ***-hospital mortality was 10.6%,and the major complication rate was 17.3%.Multivariate analysis identified diabetes mellitus(odds ratio(OR)=1.985),increased creatinine(OR=1.007),New York Heart Association(NYHA)Class III(OR=1.408),NYHA class IV(OR=1.955),cardiogenic shock(OR,6.271),and preoperative intra-aortic balloon pump insertion(OR=3.426)as independent predictors of in-hospital *** In senior-aged patients,preoperative evaluation should be carefully performed,and strict management of reversible factors needs more ***-aged patients commonly have a more severe disease status combined with more frequent comorbidities,which may lead to a high risk in mortality.

关键词： Western analysis of a large national database Outcomes of cardiac surgery in senior aged patients with ventricular dysfunction

基于新型粒子群算法的泵送射孔井口压力预测方法研究

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测井技术 2024年第3期48卷 395-401页

作者：刘明明宋智勇马收魏玉华庄欢乐北京科技大学土木与资源工程学院北京100083 中国石化华东石油工程有限公司华美孚泰公司北京100101

泵送射孔施工广泛应用于页岩气储层水力压裂技术中,但目前缺少有关泵送射孔井口压力计算的研究。基于间隙流的伯努利方程,提出了泵送摩阻模型,结合沿程摩阻和射孔摩阻建立泵送射孔井口压力数学模型,采用新型粒子群算法与最小二乘法,建... 详细信息

泵送射孔施工广泛应用于页岩气储层水力压裂技术中,但目前缺少有关泵送射孔井口压力计算的研究。基于间隙流的伯努利方程,提出了泵送摩阻模型,结合沿程摩阻和射孔摩阻建立泵送射孔井口压力数学模型,采用新型粒子群算法与最小二乘法,建立基于射孔炮眼特征及裂缝压力的泵送射孔井口压力自动拟合方法,并通过分析某井连续4段泵送射孔施工数据进行方法验证。研究发现,提出的井口压力历史拟合方法的拟合均方误差为1.29,摩阻模型计算得到泵送射孔时炮眼摩阻、沿程摩阻与泵送摩阻比例约为17∶3∶1;泵送射孔与压裂施工结束时间间隔长时,裂缝压力、流量系数及炮眼开启数量降低,炮眼摩阻、泵送压力增加。提出的方法可以有效预测泵送射孔时的井口压力,同时泵送射孔与压裂施工间隔时间长时,井下裂缝已闭合,而时间间隔短时井下裂缝仍然开启。因此,为避免射孔泵送过程中,闭合裂缝突然张开导致的压力突降,推荐压裂后立即进行泵送射孔施工。建立的泵送射孔井口压力模型可以为泵送施工提供更准确的压力预测,对指导泵送射孔施工具有重要意义。

关键词：泵送射孔井口压力粒子群算法压力预测页岩气储层

Effects of Root Pruning on Non-Hydraulic Root-Sourced Signal, Drought Tolerance and Water Use Efficiency of Winter Wheat

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Journal of Integrative Agriculture 2013年第6期12卷 989-998页

作者： ma shou-chen LI Feng-min YANG Shen-jiao LI Chun-xi XU Bing-cheng ZHANG Xu-cheng State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau Ministry of Water Resources/Institute of Soil and Water Conservation Chinese Academy of Sciences School of Surveying and Land Information Engineering Henan Polytechnic University Key Laboratory of Efficient Utilization of Water in Dry Farming Gansu Province College of Life Science Henan Normal University

Two pot experiments were conducted to study the effects of root pruning at the stem elongation stage on non-hydraulic root-sourced signals （nHRS）, drought tolerance and water use efficiency of winter wheat （Triticum aestivum）. The root pruning significantly reduced the root weight of wheat, but had no effect on root/shoot ratio at the two tested stages. At booting stage, specific root respiration of root pruned plants was significantly higher than those with intact roots （1.06 and 0.94 mmol g-1 s-1, respectively）. The soil water content （SWC） at which nHRS for root pruned plants appeared was higher and terminated lower than for intact root plants, the threshold range of nHRS was markedly greater for root pruned plants （61.1-44.6% field water capacity） than for intact root plants （57.9-46.1% field water capacity）. At flowering stage, while there was no significant difference in specific root respiration. The SWCs at which nHRS appeared and terminated were both higher for root pruned plants than for intact root plants. The values of chlorophyll fluorescence parameters, i.e., the effective photosystem II quantum yield （F PS II ）, the maximum photochemical efficiency of PS II （F v /F m ）, coefficient of photochemical quenching （qP）, and coefficient of non-photochemical quenching （NPQ）, in root pruned plants were significantly higher than in intact root plants, 7 d after withholding of water. Root pruned plants had significantly higher water use efficiency （WUE） than intact root plants in well-watered and medium drought soil, but not in severe drought condition. In addition, root pruning had no significant effect on grain yield in well-watered and medium drought soil, but significantly decreased grain yield in severe drought condition. In conclusion, the current study showed that root pruning significantly altered nHRS sensitivity and improved WUE of winter wheat in well-watered and medium drought soil, but lowered drought tolerance of winter wheat in severe drought soil

关键词： winter wheat root pruning non-hydraulic root-sourced signal (nHRS) root activity drought tolerance

机载130 mJ激光照射器的脉冲驱动电源设计

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激光杂志 2024年第9期45卷 14-18页

作者：郭俊超韩耀锋张晓辉李龙骧王诚寿少峻马世伟孙翌乔西安应用光学研究所西安710065

针对机载130 mJ激光照射器对脉冲驱动电源的实际使用需求,设计了一款高可靠性、宽温度范围、小型化的脉冲驱动电源。采用了基于超低ESR储能单元的前级低功率充电、后级高功率脉冲放电的技术方案。详细设计中,防反及缓启电路采用双PMOS... 详细信息

针对机载130 mJ激光照射器对脉冲驱动电源的实际使用需求,设计了一款高可靠性、宽温度范围、小型化的脉冲驱动电源。采用了基于超低ESR储能单元的前级低功率充电、后级高功率脉冲放电的技术方案。详细设计中,防反及缓启电路采用双PMOS管的方式;BOOST升压式充电电路选用具有开关频率可调、逐周期电流限制的控制芯片CYT5022JVS;储能单元由多只超低ESR的高分子固态铝电解电容器并联构成;放电电路开关管选用两只2.3 mΩ导通内阻的IRF150P220并联来降低发热功率。为保证宽温工作范围,采用电压补偿的方式来解决高、低温下储能单元容值变化的问题。测试表明,驱动电源能够输出峰值160 A、脉宽230μs、频率25 Hz的脉冲电流,激光照射器在照射模式下单脉冲平均能量大于130 mJ,满足系统要求。

关键词：激光技术驱动电源 BOOST变换器储能单元激光照射器

IGHG1 promotes motility likely through epithelial-mesenchymal transition in ovarian cancer

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Chinese Journal of Cancer Research 2018年第2期30卷 282-290页

作者： Jingfeng Qian Fangxing Ji Xue Ye Hongyan Cheng Ruiqiong ma Xiaohong Chang Chengchao shou Heng Cui Center of Gynecological Oncology Department of Obstetrics and Gynecology Peking University People's Hospital Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing) Department of Biochemistry and Molecular BiologyPeking University Cancer Hospital & Institute

Objective： Ovarian cancer（OC） is one of the leading causes of death for female cancer patients. COC166-9 is an OC-specific monoclonal antibody and we have identified immunoglobulin γ-1 heavy chain constant region（IGHG1） as its antigen. We explore the function of IGHG1 in proliferation, apoptosis and motility of OC cells further in this ***： IGHG1 expression in OC specimens was detected through immunohistochemistry. Real-time quantitative polymerase chain reaction（RT-q PCR） or western blotting assay was used to test IGHG1 expression in OC cells. Viability of OC cells was tested by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT）assay. Flow cytometry or western blotting assay was used to detect cell cycle and apoptosis. Cellular motility was analyzed by using transwell assay and the markers of epithelial-mesenchymal transition（EMT） were tested through ***： Although it exerts negligible effect on the viability and apoptosis of OC cells, IGHG1 could promote migration and invasion of malignant cells in vitro. Mechanistically, IGHG1 increases the expression of N-cadherin and Vimentin while decreases E-cadherin expression. Additionally, IGHG1 expression in OC specimens is higher relative to the paired normal counterparts. Further analysis demonstrates that the increased IGHG1 expression correlates positively with the lymph node metastasis of ***： IGHG1 promotes the motility of OC cells likely through executing the EMT program. Increased IGHG1 expression in OC specimens is associated with the lymph node metastasis.

关键词： IGHG1 ovarian cancer EMT invasion metastasis

Experimental Study on Sloshing Characteristics in the Elastic Tank Based on Morlet Wavelet Transform

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China Ocean Engineering 2018年第4期32卷 400-412页

作者： JIANG Mei-rong ZHONG Wen-jun YU Jian-xing LIU Pei-lin YIN Han-jun WANG shou-dong ma Yu-xiang State Key Laboratory of Hydraulic Engineering Simulation and Safety Tianjin UniversityTianjin 300072China Offshore Oil Engineering Co. Ltd.CNOOCTianjin 300451China CNOOC Research Institute Co. Ltd.CNOOCBeijing 100028China Transport Planning and Research Institute Ministry of TransportBeijing 100028China State Key Laboratory of Coastal and Offshore Engineering Dalian University of TechnologyDalian 116024China

Hydroelastic effect of sloshing is studied through an experimental investigation. Different excitation frequencies are considered with low-fill-depth and large amplitude. Morlet wavelet transform is introduced to analyze the free surface elevations and sloshing pressures. It focuses on variations and distributions of the wavelet energy in elastic tanks. The evolutions of theoretical and experimental wavelet spectra are discussed and the corresponding Fourier spectrums are compared. Afterwards, average values of the wavelet spectra are extracted to do a quantitative study at various points. From the wavelet analysis, sloshing energies are mainly distributed around the external excitation frequency and expanded to high frequencies under violent condition. In resonance, experimental wavelet energy of the elevation in elastic tanks is obviously less than that in the rigid one; for sloshing pressures, the elastic wavelet energy close to the rigid one and conspicuous impulse is observed. It recommends engineers to concern the primary natural frequency and impulsive peak pressures.

关键词： sloshing elastic tank wavelet transform free surface elevation pressure