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Improved Protein Transfer Efficiency and Signal Intensity in BlotMan Using Pulse Width Modulation

Journal of Biomedical Science and Engineering 2016年第5期9卷 269-279页

作者： thuc nhi truongvo Feixue Zheng Stanley Chien Hiroki Yokota Department of Biomedical Engineering Indiana University Purdue University Indianapolis Indianapolis IN USA YC Bioelectric LLC Indianapolis IN USA Department of Electrical and Computer Engineering Indiana University Purdue University Indianapolis Indianapolis IN USA

BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs pulse-width-modulated (PWM) voltage that applies a higher average voltage to a larger protein species. BlotMan can be controlled not only by its custom-made interface but also by a smart phone via Bluetooth technology. In this study, we examined effects of PWM signals (50%, 60%, and 80% duty cycle) on transfer efficiency and signal intensity in comparison to a constant voltage signal (100% duty cycle). The result revealed that in response to the same average voltage of 150 V, a lower duty cycle with a higher maximum voltage increased transfer efficiency as well as sharpness of transferred proteins. We validated BlotMan’s capability using a chondrosarcoma cell line (SW1353 cells) and a breast cancer cell line (MDA-MB231 cells) in response to antitumor chemical agents. BlotMan successfully generated 5 membranes from a single gel and detected 5 protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2), phosphorylated eIF2, lamin B, and actin. Collectively, we demonstrated herein that BlotMan reduces an amount of protein samples by generating multiple membranes from a single gel and improving signal intensity with PWM voltage signals.

关键词： Pulse Width Modulation (PWM) Western Blotting Protein Transfer

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Type 2 diabetes among Asian Americans:Prevalence and prevention

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World Journal of Diabetes 2015年第4期6卷 543-547页

作者： Tam H Nguyen thuc-nhi Nguyen Taylor Fischer Won Ha Thanh V Tran William F.Connell School of Nursing Boston College Graduate School of Social Work Boston College Graduate School of Social Work Library Boston College

Type 2 diabetes mellitus(T2DM) is a growing problem among Asian *** on the Centers for Disease Control,the age-adjusted prevalence of T2 DM for Asian Americans is 9%,placing them at "moderate risk".However differential patterns of disease burden emerge when examining disaggregated data acrossAsian American ethnic groups; with Filipino,Pacific Islander,Japanese,and South Asian groups consistently described as having the highest prevalence of T2 *** and strengthening prevalence data is vital for on-going prevention *** strongest evidence currently available to guide the prevention of T2 DM in the United States comes from a large multicenter randomized clinical control trial called the Diabetes Prevention Program,which targets individual lifestyle behavior *** has been translated and adopted for some Asian American groups,and shows *** stronger study designs and attention to several key methodological considerations will improve the *** attention has also been directed toward population level downstream prevention *** an infrastructure that includes both individual and population approaches is needed to prevent T2 DM among Asian American populations,and is essential for reducing health disparities.

关键词： Type 2 diabetes mellitus Asian American Prevalence Prevention Health disparity

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