检索结果-南通市图书馆

Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level

Light(Science & Applications) 2013年第1期2卷 154-162页

作者： Zhen Ma Qiuying Liu Huaxiao Yang Raymond B Runyan Carol A Eisenberg Meifeng Xu thomas k borg Roger Markwald Yifei Wang Bruce Z Gao Department of Bioengineering and COMSET Clemson UniversityClemsonSC 29634USA Department of Bioengineering University of CaliforniaBerkeleyBerkeleyCAUSA Biomedical R&D Center Jinan UniversityGuangzhouChina Department of Cellular and Molecular Medicine University of ArizonaTucsonAZ 85724USA New York Medical College/Westchester Medical Center Stem Cell Laboratory New York Medical CollegeValhallaNew YorkUSA Department of Pathology and Laboratory Medicine University of Cincinnati Medical CenterCincinnatiOHUSA Department of Regenerative Medicine and Cell Biology Medical University of South CarolinaCharlestonSCUSA

Mesenchymal stem cells(MSCs)have been cited as contributors to heart repair through cardiogenic differentiation and multiple cellular interactions,including the paracrine effect,cell fusion,and mechanical and electrical *** to heart–muscle complexity,progress in the development of knowledge concerning the role of MSCs in cardiac repair is heavily based on MSC–cardiomyocyte *** conventional coculture systems,however,the in vivo cardiac muscle structure,in which rod-shaped cells are connected end-to-end,is not sustained;instead,irregularly shaped cells spread randomly,resulting in randomly distributed cell ***,contact-mediated cell–cell interactions(e.g.,the electrical triggering signal and the mechanical contraction wave that propagate through MSC–cardiomyocyte junctions)occur ***,the data generated on the beneficial effects of MSCs may be irrelevant to in vivo biological *** this study,we explored whether cardiomyocyte alignment,the most important phenotype,is relevant to stem cell cardiogenic ***,we report(i)the construction of a laser-patterned,biochip-based,stem cell–cardiomyocyte coculture model with controlled cell alignment;and(ii)single-cell-level data on stem cell cardiogenic differentiation under in vivo-like cardiomyocyte alignment conditions.

关键词： cardiogenic differentiation mesenchymal stem cells microenvironment optical force single-cell analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：