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Advances in Bioscience and Biotechnology 2014年第1期5卷 73-80页

作者： thomas r. H. Büch Mar.us Gr.elinski Olaf Pinkenbur. thomas Guder.ann Achim Aigner r.dolf-Boehm-Institute for.Phar.acology and Toxicology Uni-versity of Leipzig Leipzig Germany Biochemia Phar.acological Institute/bpc Philipps-University Marburg Marburg Germany r.dolf-Boehm-Institute for.Phar.acology and Toxicology University of Leipzig Leipzig Germany Biochemia Phar.acological Institute/bpc Philipps-University Marburg Marburg Germany r.dolf-Boehm-Institute for.Phar.acology and Toxicology Uni-versity of Leipzig Leipzig Germany Biochemia Phar.acological Institute/bpc Philipps-University Marburg Marburg Germany Pr.sent Addr.ss: Institute for.Immunology Philipps-University Marburg Marburg German r.dolf-Boehm-Institute for.Phar.acology and Toxicology Uni-versity of Leipzig Leipzig Germany Biochemia Phar.acological Institute/bpc Philipps-University Marburg Marburg Germany Pr.sent Addr.ss: Tar.os Molecular.Pathology GmbH Kassel Germany Walther.Str.ub Institute of Phar.acology and Toxicology University of Munich (LMU) Munich Germany Compr.hensive Pneumology Center.Munich (CPC-M) German Center for Lung Research Munich Germany

In small cell lung cancer.cells, var.ous autocr.ne stimuli lead to the par.llel activation of Gq/11 and G12/13 pr.teins. The contr.bution of the Gq/11-PLC-β cascade to the mitogenic effects in SCLC cells is well established, but the r.levance of G12/13 signaling is less explor.d. While in pr.state and br.ast cancer. G12/13 activation has been shown pr.viously to pr.mote invasiveness without being involved in cellular.pr.lifer.tion, pr.vious data fr.m our.gr.up indicate anti-pr.lifer.tive effects of G12/13 knockdown in small cell lung cancer.(SCLC) cells. To fur.her.investigate the r.le of G12/13-dependent signaling in lung tumor.cells, we employed shr.A-mediated tar.eting of Gα12, Gα13, or.both, in SCLC and NSCLC cell lines. Lentivir.l expr.ssion of shr.As r.sulted in specific Gα12 and Gα13 knockdown. Of note, upon single knockdown of one family member. no counter.upr.gulation of the other.one was obser.ed. Inter.stingly, inhibition of pr.lifer.tion was cell line dependent. In cell lines wher. knock-down led to antipr.lifer.tion, single knockdown of either.Gα12 or.Gα13 was sufficient to impair.pr.lifer.tion and double knockdown of Gα12 and Gα13 tended not to fur.her.incr.ase anti-pr.lifer.tive effects. Likewise, when single knockdown was insufficient for.an inhibition of pr.lifer.tion, no effects wer. obser.ed in double knockdowns. Taken together. these findings indicate that both Gα12 and Gα13 affect cellular.pr.lifer.tion individually and inter.er.nce with one family member.is sufficient for.anti-tumor.effects.

关键词： KEYWOr.S Galpha12 Galpha13 G12/13 Lung Cancer SCLC NSCLC Gene Knockdown

mDAG: A web tool for.analyzing, visualizing, and inter.r.ting r.sponse patter.s in gene expr.ssion data with multiple tr.atments

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Advances in Bioscience and Biotechnology 2013年第6期4卷 706-709页

作者： Nam S. Vo thomas r. Sutter.Vinhthuy Phan Depar.ment of Biological Sciences the University of Memphis Memphis USA Depar.ment of Computer.Science the University of Memphis Memphis USA

Backgr.und: We pr.viously intr.duced a method based on post hoc pair.ise compar.sons to analyze gene expr.ssion r.sponses. This method utilized dir.cted gr.phs to r.pr.sent gene r.sponse to all tr.atment pair.. It has been found useful in identifying str.ctur.-activity r.lationships among dr.gs and differ.ntiating genes shar.ng similar.functional pathways. Dir.cted gr.phs ar. descr.ptive, visually expr.ssive and can benefit subsequent functional analysis. r.sults: mDAG is a web-based softwar. package based on this established method for.the analysis, visualization, and inter.r.tation of patter.s of r.sponses in gene expr.ssion data involving multiple tr.atments. Genes with the same dir.cted gr.ph patter.s hypothetically shar. similar.biological function, which may be fur.her.analyzed using exter.al tools. To facilitate subsequent functional analysis, sever.l well-known tools have been incor.or.ted into mDAG to help user. explor. hypotheses about gene function and r.gulation. This tool is useful for.any studies that analyze compar.tively r.sponse patter.s in gene expr.ssion data with multiple tr.atments (chemicals, cell types, etc.). Availability: The (ser.er.per.onal/demo) softwar. is fr.ely available at http://***. ***/mdag.

关键词： Gene Expr.ssion Dir.cted Gr.ph Pair.ise Compar.son

Methods for.Measur.ng Diffusion of a Social Media-Based Health Inter.ention

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Social Networ.ing 2015年第2期4卷 41-46页

作者： Sean D. Young thomas r. Belin Jeffr.y D. Klausner.thomas W. Valente UCLA Center.for.Digital Behavior Los Angeles USA Depar.ment of Biostatistics University of California Los Angeles Los Angeles USA Depar.ment of Medicine University of California Los Angeles Los Angeles USA Depar.ment of Pr.ventive Medicine USC Keck School of Medicine Los Angeles USA

This study evaluated the feasibility of measur.ng diffusion fr.m a social networ.ing community-level inter.ention. One year.after.completion of a r.ndomized contr.lled HIV pr.vention tr.al on Facebook, 112 minor.ty men who have sex with men (MSM) wer. asked to r.fer.Afr.can-Amer.can and/or.Latino sex par.ner. to complete a sur.ey. r.sults suggest that, compar.d to non-r.fer.er., r.fer.er. spent mor. time online, contr.lling for.age, r.ce, education, and condition. Over.60% of r.fer.als r.por.ed hear.ng about the inter.ention, and over.half r.por.ed that the r.fer.er.talked to them about changing health behavior.. r.sults pr.vide suppor. and initial feasibility of using social networ.ing for.diffusing community-based HIV inter.entions.

关键词： HIV Pr.vention Social Networ.ing Technologies Diffusion of Innovations

Satellite communications fundamentals, ò?ì 1

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Pseudophacomor.hic Glaucoma Along with Pupillar. Block after.Visian<sup>TM</sup>Implantable Collamer.Lens Implantation for.High Myopia

2000年

作者： Jules E. Kadish thomas W. r. East

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Open Jour.al of Ophthalmology 2014年第4期4卷 107-111页

作者： Michael V. McCaughey thomas r. Mifflin Car.ton r. Fenzl Jason A. Goldsmith Majid Moshir.ar Univer.ity of New Mexico School of Medicine Albuquerque USA John A. Mor.n Eye Center University of Utah Salt Lake City USA Fr.ncis I. Pr.ctor.Foundation University of California San Francisco San Francisco USA

Pur.ose: To r.por. a case of bilater.l glaucoma r.lated to pseudophacomor.hic mechanism in one eye and pupillar. block in the other.eye after.Visian Implantable Collamer.Lens (ICL;STAAr.Sur.ical) inser.ion. Methods: A 44-year.old female with high myopia under.ent bilater.l ICL implantation of MICL12.6 after.sulcus diameter.measur.ments wer. per.or.ed by Pentacam. r.sults: Pseudophacomor.hic glaucoma-r.lated angle closur. occur.ed due to lens over.izing in the r.ght eye. The mechanism was r.lieved via ICL explantation. In the left eye, pupillar. block developed in a subacute manner.after.closur. of the Per.pher.l Ir.dotomy (PI). The attack was amelior.ted by r.establishing patency of the ir.dotomy. Conclusions: ICL-r.lated glaucomatous attacks may r.sult fr.m impr.per.sizing as well as fr.m placement of a single PI. Identification of the pr.per.mechanism is vital as tr.atments differ.significantly. In pseudo phacomor.hic glaucoma, explantation is needed. In pupillar. block glaucoma, tr.atment involves establishment of a patent PI.

关键词： Pseudophacomor.hic Glaucoma Visian ICL Pupillar. Block

Cr.stal Str.ctur. r.finement: A Cr.stallogr.pher.s Guide to SHELXL

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丛书名： IUCr.texts on cr.stallogr.phy

2006年

作者： Peter.Müller.r.gine Her.st-Ir.er.Anthony L. Spek thomas r. Schneider.Michael r. Sawaya

ISBN: (数字)9780191718809

ISBN: (纸本)9780198570769

This book cover. advanced aspects of pr.ctical cr.stal str.ctur. r.finement, focusing on pr.ctical pr.blems in the ever.day life of a cr.stallogr.pher. After.an intr.duction to SHELXL in the fir.t chapter. the second chapter.pr.vides a br.ef sur.ey of cr.stal str.ctur. r.finement. The next few chapter. addr.ss the var.ous aspects of str.ctur. r.finement, fr.m the tr.atment of hydr.gen atoms to the assignment of atom types, to disor.er. to non-cr.stallogr.phic symmetr. and twinning. One chapter.is dedicated to the r.finement of macr.molecular.str.ctur.s and two shor.er.chapter. deal with str.ctur. validation. In most chapter., the book gives r.finement examples, based on the pr.gr.m SHELXL, descr.bing ever. pr.blem in detail.

关键词： cr.stal str.ctur. deter.ination SHELXL r.finement hydr.gen atoms atom types disor.er twinning pseudo-symmetr. ar.efacts str.ctur. validation cr.stal str.ctur. deter.ination SHELXL r.finement hydr.gen atoms atom types disor.er twinning pseudo-symmetr. ar.efacts str.ctur. validation

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胚胎内耳葡萄糖胺聚糖的组织化学观察

中华耳鼻咽喉头颈外科杂志 1990年第2期25卷 113-113,129页

作者：苏鸿禧 thomas r. Van De Water 北京市耳鼻咽喉科研究所美国爱因斯坦医学院耳发育生物学实验室

葡萄糖胺聚糖(Glycosaminoglycan)是胚胎细胞外基质的主要成分,是细胞繁殖、移行、发育和分化的重要因素。哺乳动物内耳发育的早期,听泡的神经外胚层被头部中胚层的疏松间充质所包裹,之后间充质发生一系列变化,包括细胞的聚集,细胞外基... 详细信息

葡萄糖胺聚糖(Glycosaminoglycan)是胚胎细胞外基质的主要成分,是细胞繁殖、移行、发育和分化的重要因素。哺乳动物内耳发育的早期,听泡的神经外胚层被头部中胚层的疏松间充质所包裹,之后间充质发生一系列变化,包括细胞的聚集,细胞外基质和耳囊软骨的形成。这些变化对软骨性耳囊作为骨迷路形成的模板和在成年骨迷路中残留软骨结构将起重要的作用。细胞外基质硫酸化葡萄糖胺聚糖的合成与耳囊

关键词：胚胎内耳葡萄糖胺聚糖

Char.cter.zation of the infectivity of an Indonesian Zika vir.s str.in in mammalian cell lines

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Asian Pacific Jour.al of Tr.pical Biomedicine 2024年第5期14卷 215-224页

作者： Justus thomas Obiajulu Siever. Anom Bowolaksono r.Tedjo Sasmono Gr.duate School of Biology Department of BiologyFaculty of Mathematics and Natural SciencesUniversitas IndonesiaDepok 16424Indonesia Eijkman r.sear.h Center.for.Molecular.Biology National Research and Innovation AgencyCibinong 16911Indonesia Cellular.and Molecular.Mechanisms in Biological System r.sear.h Gr.up Department of BiologyFaculty of Mathematics and Natural SciencesUniversitas IndonesiaDepok 16424Indonesia

Objective:To char.cter.ze the infection patter.s and gr.wth char.cter.stics of the Zika vir.s(ZIKV)str.in JMB-185 fr.m Indonesia in var.ous mammalian cell ***:ZIKV was gr.wn in human(A549,HEK293,HepG2,Huh7,Jur.at,and THP-1)and non-human mammalian(r.W264.7,Ver.,and Ver.76)cell *** r.plication kinetics wer. measur.d using plaque assay,while intr.-and extr.cellular.vir.l r.A concentr.tions wer. assessed using *** cytometr. was used to quantify the infected cells and cell viability was measur.d using an MTT *** ability of ZIKV to infect cell lines was visualized using a fluor.scence immunostaining ***:This ZIKV str.in pr.fer.ntially infected the lung,kidney,and liver.cell lines A549,HEK293,Huh7,Ver.,and Ver.76,but not the immune cells Jur.at,r.W264.7,and *** contr.st,the ZIKV showed no sign of infection in HepG2 cells,while maintaining vir.l titer.over.3 days post-infection,with no infection r.cor.ed in immunostaining,no incr.ase in vir.l r.A,and no indication of cell ***:The Indonesian ZIKV str.in has a similar.infection pr.file as other.str.ins,except for.its poor.infectivity on HepG2 *** on the gr.wth char.cter.stics of Indonesia ZIKV will help expand our.under.tanding of the biology of ZIKV which will be useful for.var.ous applications including antivir.l discover..

关键词： Zika vir.s Infectivity r.plication Cell lines In vitr.

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关于基体中马氏体转变对硬涂层的影响

热处理技术与装备 1980年第2期 12-14页

作者： thomas r. JOHANESSON

用化学气相沉积法对钻进行了TiC涂覆。由于钴内面心立方晶格转变为密集六方晶格而产生的体积变化将会对涂层施加应力。这种应力导致涂层中产生垂直于基体—涂层界面的裂纹。垂直裂纹对交界面上的微孔产生应力集

关键词：垂直裂纹涂层微孔交界面应力强度因子力学因子马氏体转变金属转变