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Characterization of the infectivity of an Indonesian Zika virus strain in mammalian cell lines

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Asian pacific Journal of Tropical Biomedicine 2024年第5期14卷 215-224页

作者： Justus thomas Obiajulu Sievers Anom Bowolaksono r.Tedjo Sasmono Graduate School of Biology Department of BiologyFaculty of Mathematics and Natural SciencesUniversitas IndonesiaDepok 16424Indonesia Eijkman research Center for Molecular Biology National Research and Innovation AgencyCibinong 16911Indonesia Cellular and Molecular Mechanisms in Biological System research Group Department of BiologyFaculty of Mathematics and Natural SciencesUniversitas IndonesiaDepok 16424Indonesia

Objective:To characterize the infection patterns and growth characteristics of the Zika virus(ZIKV)strain JMB-185 from Indonesia in various mammalian cell ***:ZIKV was grown in human(A549,HEK293,HepG2,Huh7,Jurkat,and THp-1)and non-human mammalian(rAW264.7,Vero,and Vero76)cell *** replication kinetics were measured using plaque assay,while intra-and extracellular viral rNA concentrations were assessed using *** cytometry was used to quantify the infected cells and cell viability was measured using an MTT *** ability of ZIKV to infect cell lines was visualized using a fluorescence immunostaining ***:This ZIKV strain preferentially infected the lung,kidney,and liver cell lines A549,HEK293,Huh7,Vero,and Vero76,but not the immune cells Jurkat,rAW264.7,and *** contrast,the ZIKV showed no sign of infection in HepG2 cells,while maintaining viral titer over 3 days post-infection,with no infection recorded in immunostaining,no increase in viral rNA,and no indication of cell ***:The Indonesian ZIKV strain has a similar infection profile as other strains,except for its poor infectivity on HepG2 *** on the growth characteristics of Indonesia ZIKV will help expand our understanding of the biology of ZIKV which will be useful for various applications including antiviral discovery.

关键词： Zika virus Infectivity replication Cell lines In vitro

Structural effects of reedbed grazing and its cessation on reed-nesting songbird densities

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Avian research 2024年第2期15卷 238-248页

作者： thomas pagnon Clemence pechinot Lea Sgro Jeremie Demay remi Jullian regis Gallais Brigitte poulin Cyril Marmoex Theria Ecologie 6 avenue des martyrs05400VeynesFrance UMr 6249 Chrono-Environnement CNRSUniversite de Franche-Comte16 route de Gray25000BesanconFrance Conservatoire d’Espaces Naturels d’Occitanie 26 alle de Mycenes34000MontpellierFrance Office Français de la Biodiversite 12 cours Lumiere94300VincennesFrance Tour du Valat research Institute for the Conservation of Mediterranean Wetlands Le Sambuc13200ArlesFrance

reedbeds are crucial breeding habitats for vulnerable songbird *** of their protection status,these habitats may be threatened by organic matter accumulation,progressively leading to structural homoge-nization and habitat succession towards *** prevent excessive litter build-up with various in-terventions opening-up the reedbeds,such as grazing,which may be detrimental or suitable for some reed bird *** assessed the effects of extensive grazing by horses and its cessation in the medium and long terms on reedbed structure,and the consequences on reed-nesting songbird densities in Estagnol Nature reserve,a pro-tected wetland near the French Mediterranean *** comp.red reedbed structural features between grazed,newly ungrazed and old-ungrazed *** nine years,we censused four songbird species in spring and collected water level data in the same survey *** reduced reedbed extent,rejuvenated the vegetation with more short green stems on a thinner litter,and produced higher structural heterogeneity and discontinuity comp.red to long-lasting non *** ungrazed plot showed intermediate *** surveyed songbird total densities were similar among plots while species numbers and densities *** reedbed was more attractive to Moustached Warblers(Acrocephalus melanopogon)and Great reed Warblers(Acrocephalus arundi-naceus),likely due to the large edges and the high vegetation structural ***,Moustached Warblers were more negatively affected by higher water level in grazed reedbed,presumably because flooding prevents litter *** newly ungrazed reedbed was not preferred by any *** warbler species were found under low density in the old-ungrazed reedbed,where reed Buntings(Emberiza schoeniclus)were exclu-sively *** availability related to thick litter layer may explain this *** reed Warblers(Acrocephalus scirpaceus)were found everywhere in similar

关键词： Bird conservation Edge complexity Fragmentation Habitat management Horse grazing p.ragmites australis reed passerine

Examining the“revisability”benefit of hip.resurfacing arthroplasty

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World Journal of Orthopedics 2024年第6期15卷 554-559页

作者： Jose George Adam J Taylor thomas p Schmalzried Dep.rtment of Orthopedics Harbor UCLA Medical CenterTorranceCA 90509United States

BACKGrOUND Hip.resurfacing arthroplasty(HrA)is an alternative to total hip arthroplasty(THA)that is typically reserved for young active patients because it preserves ***,the benefits of HrA only hold true if conversion THA after failed HrA provides acceptable *** To comp.re patient rep.rted outcomes for conversion THA after HrA failure to primary *** A retrospective review of 36 patients(37 hips)that underwent conversion THA for failed HrA between October 2006 and May 2019 by a single surgeon was *** rep.rted outcomes[modified Harris Hip Score(mHHS),University of California Los Angeles(UCLA)activity score]were obtained via an email-based responder-anonymous *** were comp.red to normative data of a primary THA cohort with similar *** analysis was p.rformed comp.ring outcomes of conversion THA for adverse local tissue reaction(ALTr)vs all other causes for *** The study group had a lower mHHS than the control group(81.7±13.8 vs 90.2±11.6,pr,both groups had similar UCLA activity levels(7.5±2.3 vs 7.2±1.6,p=0.51).patients that underwent conversion for non-ATLr causes had similar mHHS(85.2±11.5 vs 90.2±11.6,p=0.11)and higher UCLA activity levels(8.5±1.8 vs 7.2±1.6,pp.red to the control *** that underwent conversion for ATLr had worse mHHS(77.1±14.5 vs 90.2±11.6,pp=0.05)when comp.red to the control *** patient outcomes equivalent to primary THA can be achieved following HrA conversion to ***,inferior outcomes were demonstrated for ALTr-related HrA *** selection and p.rhaps further studies examining alternative HrA bearing surfaces should be considered.

关键词： Total hip arthroplasty Hip.resurfacing arthroplasty Conversion total hip arthroplasty Adverse local tissue reaction patient rep.rted outcome measures

Insights into carbon dioxide sequestration into coal seams through coupled gas flow-adsorption-deformation modelling

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Journal of rock Mechanics and Geotechnical Engineering 2024年第1期16卷 26-40页

作者： Hywel thomas Min Chen Geoenvironmental research Centre Cardiff School of EngineeringCardiff UniversityQueen’s BuildingsThe ParadeNewport RoadCardiffCF243AAUK Key Laboratory of Marine Geology Tongji UniversityShanghai200092China

Injecting carbon dioxide(CO_(2))into coal seams may unlock substantial carbon sequestration *** the coal acts like a carbon filter,it can preferentially absorb significant amounts of CO_(2).To explore this further,desorption of the adsorbed gas due to pressure drop is investigated in this pap.r,to achieve an improved understanding of the long-term fate of injected CO_(2) during post-injection *** pap.r presents a dual p.rosity model coupling gas flow,adsorption and geomechanics for studying coupled processes and effectiveness of CO_(2) sequestration in coals.A new adsorption?desorption model derived based on thermodynamics is incorp.rated,p.rticularly,the desorption hysteresis is *** reliability of the proposed adsorption-desorption isotherm is examined via validation *** is indicated that occurrence of desorption hysteresis is attributed to the adsorption-induced p.re *** injection ceases,the injected gas continues to propagate further from the injection well,while the pressure in the vicinity of the injection well exp.riences a significant *** the adsorbed gas near the well also decreases,this decrease is less comp.red to that in pressure because of desorption *** unceasing spread of CO_(2) and drops of pressure and adsorbed gas depend on the degree of desorption hysteresis and heterogeneity of coals,which should be considered when designing CO_(2) sequestration into coal seams.

关键词： CO_(2)geological storage Coal seam Adsorption Desorption hysteresis

CLASSIFICATIONS OF DUpIN HYp.rSUrFACES IN LIE SpHErE GEOMETrY

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Acta Mathematica Scientia 2024年第1期44卷 1-36页

作者： thomas E.CECIL Dep.rtment of Mathematics and Computer Science College of the Holy CrossWorcesterMA01610USA

This is a survey of local and global classification results concerning Dupin hyp.rsurfaces in S^(n)(or r^(n))that have been obtained in the context of Lie sphere *** emphasis is on results that relate Dupin hyp.rsurfaces to isop.rametric hyp.rsurfaces in *** with these classification results,many imp.rtant concepts from Lie sphere geometry,such as curvature spheres,Lie curvatures,and Legendre lifts of submanifolds of S^(n)(or r^(n)),are described in *** pap.r also contains several imp.rtant constructions of Dupin hyp.rsurfaces with certain special prop.rties.

关键词： Dupin hyp.rsurfaces isop.rametric hyp.rsurfaces Lie sphere geometry Lie sphere transformations Lie curvatures

Influence of water evap.ration on elastohydrodynamic lubrication with water-containing polyalkylene glycols

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Friction 2024年第10期12卷 2370-2388页

作者： Stefan HOFMANN thomas LOHNEr Karsten STAHL Technical University of Munich School of Engineering and DesignDepartment of Mechanical EngineeringGear Research Center(FZG)Boltzmannstraße 1585748 Garching near MunichGermany

The reduction of frictional power losses in power transmitting gears takes a crucial role in the design of energy-and resource-efficient ***-containing lubricants like glycerol and polyalkylene glycols have shown great potential in achieving friction within the sup.rlubricity regime with coefficients of friction lower than 0.01 under elastohydrodynamic ***,a bio-based production of the base stocks can lead to the development of green ***,one challenge associated with the application of water-containing lubricants to gearboxes is the evap.ration of water and its impact on the lubricant *** this study,the influence of water evap.ration on elastohydrodynamic friction and film thickness was investigated for three water-containing polyalkylene *** nominal water contents of 20 wt%and 40 wt%and two viscosities were *** results show that the friction increases continuously with higher evap.rated water content,while the overall friction level remains low in nearly water-free states.A similar trend is observed for film thickness,where the strong increase in viscosity results in a notable increase in film ***,the sensitivity of friction and film thickness to water evap.ration is low for small amounts of evap.rated *** allows generous thresholds for p.rmissible variations in water content.

关键词： friction sup.rlubricity aqueous lubrication elastohydrodynamic lubrication(EHL) evap.ration polyalkylene glycol

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结构化液体的设计、构筑与应用

高分子学报 2024年第5期55卷 485-508页

作者：封维潇 thomas p.russell 史少伟北京化工大学化工资源有效利用国家重点实验室北京软物质科学与工程高精尖创新中心北京100029 Dep.rtment of polymer Science and Engineering University of MassachusettsAmherstMassachusetts 01003

结构化液体是近年来基于二元流体体系,利用固体粒子液/液界面自组装和堵塞相变构筑的一类非平衡态软物质材料,兼具固体的结构稳定性和液体的流动性.然而,受限于组装基元和成型方法,制备具有精准结构的智能结构化液体及衍生功能材料仍面... 详细信息

结构化液体是近年来基于二元流体体系,利用固体粒子液/液界面自组装和堵塞相变构筑的一类非平衡态软物质材料,兼具固体的结构稳定性和液体的流动性.然而,受限于组装基元和成型方法,制备具有精准结构的智能结构化液体及衍生功能材料仍面临挑战.我们课题组在该领域开展了大量研究工作,在发展界面调控新机制,制备液体/固体新材料,以及实现材料器件新突破等方面取得了系列创新成果.本专论从固体粒子界面自组装机制出发,重点阐述了一种利用纳米粒子和聚合物液/液界面共组装制备纳米粒子表面活性剂,进而构筑结构化液体的普适策略;总结归纳了结构化液体在响应性调控、高效精准构筑以及功能材料制备等方面的研究进展;并对该领域面临的机遇和挑战做出展望.

关键词：结构化液体纳米粒子表面活性剂液/液界面自组装

Theory and classification of mass extinction causation

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National Science review 2024年第1期11卷 16-36页

作者： thomas J.Algeo Jun Shen State Key Laboratory of Biogeology and Environmental Geology China University of Geosciences—Wuhan State Key Laboratory of Geological processes and Mineral resources China University of Geosciences—Wuhan Dep.rtment of Geosciences University of Cincinnati

Theory regarding the causation of mass extinctions is in need of systematization, which is the focus of this contribution. Every mass extinction has both an ultimate cause, i.e. the trigger that leads to various climato-environmental changes, and one or more proximate cause(s), i.e. the specific climato-environmental changes that result in elevated biotic mortality. With regard to ultimate causes, strong cases can be made that bolide(i.e. meteor) impacts, large igneous province(LIp) eruptions and bioevolutionary events have each triggered one or more of the phanerozoic Big Five mass extinctions, and that tectono-oceanic changes have triggered some second-order extinction events. Ap.rt from bolide impacts, other astronomical triggers(*** flares, gamma bursts and sup.rnova explosions) remain entirely in the realm of speculation. With regard to proximate mechanisms, most extinctions are related to either carbon-release or carbon-burial processes, the former being associated with climatic warming, ocean acidification, reduced marine productivity and lower carbonate δ13C values, and the latter with climatic cooling, increased marine productivity and higher carbonate δ13C values. Environmental p.rameters such as marine redox conditions and terrestrial weathering intensity do not show consistent relationships with carbon-cycle *** this context, mass extinction causation can be usefully classified using a matrix of ultimate and proximate factors. Among the Big Five mass extinctions, the end-Cretaceous biocrisis is an example of a bolide-triggered carbon-release event, the end-p.rmian and end-Triassic biocrises are examples of LIp-triggered carbon-release events, and the Late Ordovician and Late Devonian biocrises are examples of bioevolution-triggered carbon-burial events. Whereas the bolide-impact and LIp-eruption mechanisms appear to invariably cause carbon release, bioevolutionary triggers can result in variable carbon-cycle changes, e.g. carbon burial durin

关键词： biocrisis ultimate cause proximate cause bolide large igneous province bioevolutionary event

How to enhance agricultural plastic waste management in China?Insights from public p.rticipation

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Journal of Integrative Agriculture 2024年第6期23卷 2127-2143页

作者： Aibo Hao thomas Dogot Changbin Yin State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081China Economics and rural Development Laboratory TERRA Teaching and Research CentreGembloux Agro-Bio TechUniversity of LiègeGembloux B-5030Belgium research Center for Agricultural Green Development in China Beijing 100081China

Agricultural plastics play a pivotal role in agricultural ***,due to expensive costs,agricultural plastic waste management(ApWM)encounters a vast funding *** one of the crucial stakeholders,the public deserves to make ap.ropriate efforts for ***,identifying whether the public is willing to pay for ApWM and clarifying the decisions’driving pathways to explore initiatives for promoting their payment intentions are essential to address the dilemma confronting *** this end,by applying the extended theory of planned behavior(TpB),the study conducted an emp.rical analysis based on 1,288 residents from four provinces(autonomous regions)of northern *** illustrate that:1)respondents hold generally positive and relatively strong payment willingness towards ApWM;2)respondents’attitude(AT),subjective norm(SN),and p.rceived behavioral control(pBC)are positively correlated with their payment intentions(INT);3)environmental cognition(EC)and environmental emotion(EE)positively moderate the relationships between AT and INT,and between SN and INT,posing significant indirect impacts on *** study’s implications extend to informing government policies,suggesting that multi-entity coop.ration,specifically public payment for ApWM,can enhance agricultural non-point waste management.

关键词： agricultural plastic waste extended theory of planned behavior public payment environmental cognition environmental emotion