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用固定化的多酶系统和葡聚糖-NAD^+的合成单元制备必需的L-支链氨基酸

第十一届国际人工血液大会

作者： thomas ming swi chang O.C. M.D.C.M.Ph.D.FRCP[C]FRS[C]DirectorArtificial Cells ＆ Organs Research CentreDepartments of PhysiologyMedicine ＆ Biomedical EngineeringFaculty of MedicineMcGill UniversityMontrealQCCanada.H3G 1Y6

＜正＞Artificial cells were first reported 50th years ago1,*** ideas are being used increasing in the modern era of biotechnology,nanomedicine,regenerative medicine,blood substitutes, cell/stem cell therapy and even nanorobatics 3,4,***,this lecture will be on blood substitutes starting with a very brief overview of hemoglobin based blood substitutes

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氨基酸杂志 1992年第1期14卷 59-62,18页

作者： Kong Fu Gu thomas ming swi chang 徐宁宁

我们制备的合成单元,每种都含有亮氨酸脱氢酶,尿素酶,可溶性的葡聚糖和下列辅酶再生脱氢酶:葡萄糖脱氢酶,酵母酒精脱氢酶、苹果酸脱氢酶或乳酸脱氢酶。合成细胞被填充在一个小柱里,随着葡聚糖-NADH的产生,L亮氨酸,L缬氨酸和L异亮氨酸相... 详细信息

我们制备的合成单元,每种都含有亮氨酸脱氢酶,尿素酶,可溶性的葡聚糖和下列辅酶再生脱氢酶:葡萄糖脱氢酶,酵母酒精脱氢酶、苹果酸脱氢酶或乳酸脱氢酶。合成细胞被填充在一个小柱里,随着葡聚糖-NADH的产生,L亮氨酸,L缬氨酸和L异亮氨酸相继产生。得率的最大值依赖于所用的辅酶产生系统。对于D葡萄糖和葡萄糖脱氢酶系统,得率为83～93％;对于乙醇和酵母酒精脱氢酶系统,得率为90％;对于L-苹果酸和苹果酸脱氢酶系统,得率为45～55％;对于L乳酸和乳酸脱氢酶系统,得率为64～78％。本文还进行了动力学实验,表观Km值如下:α-酮异己酸(KIC),0.33mM;α-酮异戍酸(KIV),0.51mM;DL α-酮-β-甲基-η-戊酮(KMV),0.58mM;尿素,3.52mM;D-葡萄糖,27.82mM;乙醇,3.89mM;L苹果酸3.02mM;L乳酸16.67mM。动力学分析表明,在亮氨酸脱氢酶催化的反应中,KIC,KIV和KMV都是竞争性抑制物,它们的抑制系数是和Km值相关的。

关键词： L-支链氨基酸多酶系统葡聚糖-NAD

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人工细胞研究

国际生物医学工程杂志 1987年第6期 328-330页

作者： thomas ming swi chang 陆茉珠

自1957年人工细胞的可行性首次被证明以来,研究探索剧增,使其趋于实用。现在利用新的合成材料或生物材料可以制备具有各种渗透性、表面性能和血液相容性的人工细胞。人工细胞内能包含许多物质。这些物质包括酶系统、细胞提取物、生物细... 详细信息

自1957年人工细胞的可行性首次被证明以来,研究探索剧增,使其趋于实用。现在利用新的合成材料或生物材料可以制备具有各种渗透性、表面性能和血液相容性的人工细胞。人工细胞内能包含许多物质。这些物质包括酶系统、细胞提取物、生物细胞、能吸引同位素的物质、抗原、抗体、疫苗、激

关键词：人工细胞酶系统包囊肝衰竭白蛋白肝昏迷肝疾病血液灌流血液相容性

A phased approach to enable hybrid simulation of complex structures

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Earthquake Engineering and Engineering Vibration 2014年第S1期13卷 63-77页

作者： Billie F.Spencer Jr. Chia-ming chang thomas M.Frankie Daniel A.Kuchma Pedro F.Silva Adel E.Abdelnaby Department of Civil Engineering University of Illinois at Urbana-Champaign Nathan M.& Anne M.Newmark Endowed Chair Earthquake Engineering Research &Test Center Guangzhou University Associate Professor Wiss JanneyElstner AssociatesInc. Department of Civil and Environmental Engineering University of Illinois at Urbana-Champaign Department of Civil and Environmental Engineering George Washington University Department of Civil Engineering University of Memphis Assistant Professor

Hybrid simulation has been shown to be a cost-effective approach for assessing the seismic performance of structures. In hybrid simulation,critical parts of a structure are physically tested,while the remaining portions of the system are concurrently simulated computationally,typically using a finite element model. This combination is realized through a numerical time-integration scheme,which allows for investigation of full system-level responses of a structure in a cost-effective manner. However,conducting hybrid simulation of complex structures within large-scale testing facilities presents significant challenges. For example,the chosen modeling scheme may create numerical inaccuracies or even result in unstable simulations; the displacement and force capacity of the experimental system can be exceeded; and a hybrid test may be terminated due to poor communication between modules(e.g.,loading controllers,data acquisition systems,simulation coordinator). These problems can cause the simulation to stop suddenly,and in some cases can even result in damage to the experimental specimens; the end result can be failure of the entire experiment. This study proposes a phased approach to hybrid simulation that can validate all of the hybrid simulation components and ensure the integrity largescale hybrid simulation. In this approach,a series of hybrid simulations employing numerical components and small-scale experimental components are examined to establish this preparedness for the large-scale experiment. This validation program is incorporated into an existing,mature hybrid simulation framework,which is currently utilized in the Multi-Axial Full-Scale Sub-Structuring Testing and Simulation(MUST-SIM) facility of the George E. Brown Network for Earthquake Engineering Simulation(NEES) equipment site at the University of Illinois at Urbana-Champaign. A hybrid simulation of a four-span curved bridge is presented as an example,in which three piers are experimentally controlle

关键词： hybrid simulation seismic performance evaluation phased approach curved bridge MUST-SIM facility

Case Report:Pulmonary actinomycosis:a case undergoing resection through video-assisted thoracic surgery (VATS)

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第10期8卷 721-724页

作者： LIN ming-shian LIN Wea-lung LUH Shi-ping TSAO thomas chang-yao WU Tzu-ching Department of Medicine Chia-Yi Christian Hospital600 Taichung Taiwan China Department of Pathology Chung-Shan Medical University and Hospital402 Taichung Taiwan China Department of Surgery Chung-Shan Medical University and Hospital402 Taichung Taiwan China Department of Medicine Chung-Shan Medical University and Hospital402 Taichung Taiwan China

Actinomycosis is an uncommon disease, which is usually manifested as cervicofacial infection and related to poor oral hygiene or compromised immune function. Pulmonary actinomycosis is rare, but its diagnosis is changing due to its variable presentation and the similarity in appearance to other intrapulmonary diseases. Here we report an 80-year-old man with a solitary pulmonary nodule over the left upper lobe. Pulmonary neoplasm was highly suspected in this patient and thus resection of the mass was undertaken through video-assisted thoracic surgery (VATS). Histopathological examination demonstrated this patient had an Actinomyeces infection. While the application of VATS in patients with pulmonary actinomycosis has rarely been reported in literature, we conclude that VATS is valuable for the diagnosis and treatment of patients with undetermined pulmonary nodule(s).

关键词： Pulmonary actinomycosis Video-assisted thoracic surgery (VATS) Resection

High-density lipoprotein regulates angiogenesis by long non-coding RNA HDRACA

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Signal Transduction and Targeted Therapy 2023年第9期8卷 4320-4337页

作者： Zhi-Wei Mo Yue-ming Peng Yi-Xin Zhang Yan Li Bi-Ang Kang Ya-Ting Chen Le Li Mary GSorci-thomas Yi-Jun Lin Yang Cao Si Chen Ze-Long Liu Jian-Jun Gao Zhan-Peng Huang Jia-Guo Zhou Mian Wang Guang-Qi chang Meng-Jie Deng Yu-Jia Liu Zhen-Sheng Ma Zuo-Jun Hu Yu-Gang Dong Zhi-Jun Ou Jing-Song Ou Division of Cardiac Surgery Cardiovascular Diseases InstituteThe First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina National-Guangdong Joint Engineering Laboratory for Diagnosis and Treatment of Vascular Diseases NHC Key Laboratory of Assisted Circulation(Sun Yat-sen University)Guangdong Provincial Engineering and Technology Center for Diagnosis and Treatment of Vascular DiseasesGuangzhouChina Division of Vascular Surgery The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Division of Hypertension and Vascular Diseases Department of CardiologyHeart CenterThe First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Medical College of Wisconsin MilwaukeeWIUSA Center for Translational Medicine Institute of Precision MedicineThe First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Department of Cardiology Heart CenterThe First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Department of Pharmacology Cardiac and Cerebral Vascular Research CenterZhongshan School of Medicine of Sun Yat-sen UniversityGuangzhouChina Guangdong Provincial Key Laboratory of Brain Function and Disease Zhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510080P.R.China

Normal high-density lipoprotein(nHDL)can induce angiogenesis in healthy ***,HDL from patients with coronary artery disease undergoes various modifications,becomes dysfunctional(dHDL),and loses its ability to promote ***,we identified a long non-coding RNA,HDRACA,that is involved in the regulation of angiogenesis by *** this study,we showed that nHDL downregulates the expression of HDRACA in endothelial cells by activating WW domain-containing E3 ubiquitin protein ligase 2,which catalyzes the ubiquitination and subsequent degradation of its transcription factor,Kruppel-like factor 5,via sphingosine 1-phosphate(S1P)receptor *** contrast,dHDL with lower levels of S1P than nHDL were much less effective in decreasing the expression of *** was able to bind to Ras-interacting protein 1(RAIN)to hinder the interaction between RAIN and vigilin,which led to an increase in the binding between the vigilin protein and proliferating cell nuclear antigen(PCNA)mRNA,resulting in a decrease in the expression of PCNA and inhibition of *** expression of human HDRACA in a hindlimb ischemia mouse model inhibited the recovery of *** together,these findings suggest that HDRACA is involved in the HDL regulation of angiogenesis,which nHDL inhibits the expression of HDRACA to induce angiogenesis,and that dHDL is much less effective in inhibiting HDRACA expression,which provides an explanation for the decreased ability of dHDL to stimulate angiogenesis.

关键词： angiogenesis lipoprotein inhibited

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人造细胞

国外科技新书评介 2008年第9期 11-11页

作者： thomas ming swi chang 徐素宏不详中国科学院遗传与发育生物学研究所博士生

本书是至今为止对人造细胞以及相关领域最为全面的概述。作为该书的作者，thomas ming swi chang，加拿大的华人科学家，有着对人造细胞深厚的造诣。从他第一次设计人造红血球细胞到现在50多年的时间里，他一直活跃于这一领域。在这本... 详细信息

本书是至今为止对人造细胞以及相关领域最为全面的概述。作为该书的作者，thomas ming swi chang，加拿大的华人科学家，有着对人造细胞深厚的造诣。从他第一次设计人造红血球细胞到现在50多年的时间里，他一直活跃于这一领域。在这本书中，作者从当初对人造细胞不可思议的想法娓娓道来；像叙述历史一样讲述了人造细胞从简单的构思到复杂的分子机制以及应用的经历，并且预测了今后人造细胞的发展方向。

关键词：人造细胞华人科学家分子机制加拿大红血球作者

Verification of gene expression profiles for colorectal cancer using 12 internet public microarray datasets

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World Journal of Gastroenterology 2014年第46期20卷 17476-17482页

作者： Yu-Tien chang Chung-Tay Yao Sui-Lung Su Yu-Ching Chou Chi-ming Chu Chi-Shuan Huang Harn-Jing Terng Hsiu-Ling Chou thomas Wetter Kang-Hua Chen Chi-Wen chang Yun-Wen Shih Ching-Huang Lai Division of Biomedical Statistics and Informatics School of Public HealthNational Defense Medical Center Department of Emergency Cathay General Hospital Department of Epidemiology School of Public HealthNational Defense Medical Center Division of Colorectal Surgery Cheng Hsin Rehabilitation Medical Center Advpharma Inc. Department of Nursing Far Eastern Memorial Hospital Oriental Institute of Technology Department of Medical Informatics Faculty of MedicineUniversity of Heidelberg69120 HeidelbergGermany School of Nursing College of MedicineChang Gung University

AIM:To verify gene expression profiles for colorectal cancer using 12 internet public microarray ***:Logistic regression analysis was performed,and odds ratios for each gene were determined between colorectal cancer(CRC)and *** microarray datasets of GSE 4107,4183,8671,9348,10961,13067,13294,13471,14333,15960,17538,and 18105,which included 519 cases of adenocarcinoma and 88 normal mucosa controls,were pooled and used to verify 17 selective genes from 3 published studies and estimate the external ***:We validated the 17 CRC-associated genes from studies by chang et al(Model 1:5 genes),Marshall et al(Model 2:7 genes)and Han et al(Model 3:5genes)and performed the multivariate logistic regression analysis using the pooled 12 public microarray datasets as well as the external *** goodnessof-fit test of Hosmer-Lemeshow(H-L)showed statistical significance(P=0.044)for Model 2 of Marshall et al in which observed event rates did not match expected event rates in subgroups of the model *** and observed event rates in subgroups were similar,which are called well calibrated,in Models 1,3and 4 with non-significant P values of 0.460,0.194 and1.000 for H-L tests,respectively.A 7-gene model of CPEB4,EIF2S3,MGC20553,MS4A1,ANXA3,TNFAIP6and IL2RB was pairwise selected,which showed the best results in logistic regression analysis(H-L P=1.000,R2=0.951,areas under the curve=0.999,accuracy=0.968,specificity=0.966 and sensitivity=0.994).CONCLUSION:A novel gene expression profile was associated with CRC and can potentially be applied to blood-based detection assays.

关键词： Gene expression profiles Colorectal cancer Microar

Gene expression profile of peripheral blood in colorectal cancer

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World Journal of Gastroenterology 2014年第39期20卷 14463-14471页

作者： Yu-Tien chang Chi-Shuan Huang Chung-Tay Yao Sui-Lung Su Harn-Jing Terng Hsiu-Ling Chou Yu-Ching Chou Kang-Hua Chen Yun-Wen Shih Chian-Yu Lu Ching-Huang Lai Chen-En Jian Chiao-Huang Lin Chien-Ting Chen Yi-Syuan Wu Ke-Shin Lin thomas Wetter Chi-Wen chang Chi-ming Chu Division of Biomedical Statistics and Informatics School of Public Health National Defense Medical Center Division of Colorectal Surgery Cheng Hsin Rehabilitation Medical Center Department of Surgery Cathay General Hospital Department of Epidemiology School of Public Health National Defense Medical Center Advpharma Inc. Department of Nursing Far Eastern Memorial Hospital and Oriental Institute of Technology Department of Nursing School of Medicine Chang Gung University Air Force Combatant Command National Defense Ministry Institute of Medical Biometry and Informatics Heidelberg University Germany Department of Biomedical Informatics and Medical Education University of Washington Seattle United States

AIM:Optimal molecular markers for detecting colorectal cancer(CRC)in a blood-based assay were ***:A matched(by variables of age and sex)case-control design(111 CRC and 227 non-cancer samples)was *** RNAs isolated from the338 blood samples were reverse-transcribed,and the relative transcript levels of candidate genes were *** training set was made of 162 random samples of the total 338 samples.A logistic regression analysis was performed,and odds ratios for each gene were determined between CRC and *** samples(n=176)in the testing set were used to validate the logistic model,and an inferred performance(generality)was *** pooling 12 public microarray datasets(GSE 4107,4183,8671,9348,10961,13067,13294,13471,14333,15960,17538,and 18105),which included 519 cases of adenocarcinoma and 88 controls of normal mucosa,we were able to verify the selected genes from logistic models and estimate their external ***:The logistic regression analysis resulted in the selection of five significant genes(P<0.05;MDM2,DUSP6,CPEB4,MMD,and EIF2S3),with odds ratios of 2.978,6.029,3.776,0.538 and 0.138,*** five-gene model performed stably for the discrimination of CRC cases from controls in the training set,with accuracies ranging from 73.9%to 87.0%,a sensitivity of 95%and a specificity of 95%.In addition,a good performance in the test set was obtained using the discrimination model,providing 83.5%ac-curacy,66.0%sensitivity,92.0%specificity,a positive predictive value of 89.2%and a negative predictive value of 73.0%.Multivariate logistic regressions analyzed 12 pooled public microarray data sets as an external *** that provided similar expected and observed event rates in subgroups were termed well calibrated.A model in which MDM2,DUSP6,CPEB4,MMD,and EIF2S3 were selected showed the result in logistic regression analysis(H-L P=0.460,R2=0.853,AUC=0.978,accuracy=0.949,specificity=0.818 and sensitivity=0

关键词： Colorectal cancer Gene expression Micro-array Inte

Panda X-III： Searching for neutrinoless double beta decay with high pressure ^(136)Xe gas time projection chambers

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Science China(Physics,Mechanics & Astronomy) 2017年第6期60卷 8-47页

作者： Xun Chen chang Bo Fu Javier Galan Karl Giboni Franco Giuliani Ling Hui Gu Ke Han Xiang Dong Ji Heng Lin Jiang Lai Liu Kai Xiang Ni Hiroki Kusano Xiang Xiang Ren Shao Bo Wang Yong Yang Dan Zhang Tao Zhang Li Zhao Xiang ming Sun Shou Yang Hu Si Yu Jian Xing Long Li Xiao Mei Li Hao Liang Huan Qiao Zhang ming Rui Zhao Jing Zhou Ya Jun Mao Hao Qiao Si Guang Wang Ying Yuan Meng Wang Amir N.Khan Neill Raper Jian Tang Wei Wang Jia Ning Dong chang Qing Feng Cheng Li Jian Bei Liu Shu Bin Liu Xiao Lian Wang Dan Yang Zhu Juan F.Castel Susana Cebri'an Theopisti Dafni Javier G.Garza Igor G.Irastorza Francisco J.Iguaz Gloria Luz ′on Hector Mirallas Stephan Aune Eric Berthoumieux Yann Bedfer Denis Calvet Nicole d'Hose Alain Delbart Maria Diakaki Esther Ferrer-Ribas Andrea Ferrero Fabienne Kunne Damien Neyret thomas Papaevangelou Franck Sabatie Maxence Vanderbroucke AnDi Tan Wick Haxton Yuan Mei Chinorat Kobdaj Yu-Peng Yan NPAC and Department of Physics and Astronomy Shanghai Jiao Tong University Shanghai Laboratory for Particle Physics and Cosmology Shanghai 200240 China College of Physical Science and Technology Central China Normal University Wuhan 430079 China China Institute of Atomic Energy Beijing 102413 China School of Physics Peking University Beijing 100871 China School of Physics and Key Laboratory of Particle Physics and Particle Irradiation (MOE) Shandong University Jinan 250100 China School of Physics and Engineering Sun Yat-Sen University Guangzhou 510275 China Department of Modern Physics University of Science and Technology of China Hefei 230026 China State Key Laboratory of Particle Detection and Electronics University of Science and Technology of China Hefei 230026 China University ofZaragoza C/P Cerbuna 12 50009 Zaragoza Spain IRFU CEA Universite Paris-Saclay F-91191 Gif-sur-Yvette France Department of Physics University of Maryland College Park MD 20742 USA Nuclear Science Division Lawrence Berkeley National Laboratory Berkeley CA 94720 USA Center for Excellence in High Energy Physics and Astrophysics Suranaree University of Technology Nakhon Ratchasima 30000 Thailand

Searching for the neutrinoless double beta decay(NLDBD)is now regarded as the topmost promising technique to explore the nature of neutrinos after the discovery of neutrino masses in oscillation *** X-III(particle and astrophysical xenon experiment III)will search for the NLDBD of136Xe at the China Jin Ping Underground Laboratory(CJPL).In the first phase of the experiment,a high pressure gas Time Projection Chamber(TPC)will contain 200 kg,90%136Xe enriched gas operated at10 *** pitch micro-pattern gas detector(Microbulk Micromegas)will be used at both ends of the TPC for the charge readout with a cathode in the *** signals can be used to reconstruct the electron tracks of the NLDBD events and provide good energy and spatial *** detector will be immersed in a large water tank to ensure～5 m of water shielding in all *** second phase,a ton-scale experiment,will consist of five TPCs in the same water tank,with improved energy resolution and better control over backgrounds.

关键词：中微子质量双β衰变影室时间熊猫高压力气体探测器能量分辨率