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Asian Journal of Andrology 2011年第3期13卷 432-437,512页

作者： thomas is hwang Tien-Ling Liao Ji-Fan Lin Yi-Chia Lin Shu-Yu Lee Yen-Chun Lai Shu-Huei Kao Department of Urology Taipei Medical University Taipei Taiwan China Department of Urology School of Medicine Fu-Jen Catholic University Taipei Taiwan China Division of Urology Department of Surgery Shin Kong Wu Ho-Su Memorial Hospital Taipei Taiwan China School of Medical Laboratory Science and Biotechnology Taipei Medical University Taipei Taiwan China Central Laboratory Shin Kong Wu Ho-Su Memorial Hospital Taipei Taiwan China

Testosterone replacement therapy has benefits for aging men and those with hypogonadism. However, the effects of exogenous testosterone on Leydig cells are still unclear and need to be clarified. In this report, we demonstrate that testosterone supplementation can reduce oxidative damage in Leydig cells. The TM3 Leydig cell line was used as an in vitrocell model in this study. Cytoprotective effects were identified with 100-nmol l-1 testosterone treatment, but cytotoxic effects were found with ≥ 500-nmol l-1 testosterone supplementation. Significantly reduced reactive oxygen species （ROS） generation, lipid peroxide contents and hypoxia induction factor （HIF）-lα stabilization and activation were found with lO0-nmol 1-1 testosterone treatment. There was a 1.72-fold increase in ROS generation in the 500-nmol l-1 compared to the lO0-nmol l-1 testosterone treatment. A 1.58-fold increase in steroidogenic acute regulatory protein （STAR） expression was found in 50-nmol l-1 testosterone-treated cells （P〈0.01）. Chemically induced hypoxia was attenuated by testosterone supplementation. Leydig cells treated with low-dose testosterone supplementation showed cytoprotection by decreasing ROS and lipid peroxides, increasing StAR expression and relieving hypoxia stress as demonstrated by HIF-1α stabilization. Increased oxidative damage was found with ≥ 500-nmol l-1 testosterone manipulation. The mechanism governing the differential dose effects of testosterone on Leydig cells needs further investigation in order to shed light on testosterone replacement therapy.

关键词： Leydig cells oxidative damage reactive oxygen species testosterone

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Neuron-to-vessel signaling is a required feature of aberrant stem cell commitment after soft tissue trauma

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Bone Research 2022年第3期10卷 615-626页

作者： Qizhi Qin Mario Gomez-Salazar Masnsen Cherief Chase A.Pagani Seungyong Lee Charles hwang Robert J.Tower Sharon Onggo Yuxiao Sun Abhinav Piplani Zhao Li Sowmya Ramesh thomas L.Clemens Benjamin Levi Aaron W.James Department of Pathology Johns Hopkins UniversityBaltimoreMD 21205USA Center for Organogenesis and Trauma Department of SurgeryUniversity of TexasSouthwesternTXUSA Department of Plastic Surgery HarvardCambridgeMA 02138USA Department of Orthopaedics Johns Hopkins UniversityBaltimoreMD 21205USA Baltimore Veterans Administration Medical Center BaltimoreMD 21201USA

The functional interdependence of nerves and blood vessels is a well-established concept during tissue morphogenesis, yet the role of neurovascular coupling in proper and aberrant tissue repair is an emerging field of interest. Here, we sought to define the regulatory relationship of peripheral nerves on vasculature in a severe extremity trauma model in mice, which results in aberrant cell fate and heterotopic ossification(HO). First, a high spatial degree of neurovascular congruency was observed to exist within extremity injury associated heterotopic ossification. Vascular and perivascular cells demonstrate characteristic responses to injury,as assessed by single cell RNA sequencing. This vascular response to injury was blunted in neurectomized mice, including a decrease in endothelial proliferation and type H vessel formation, and a downregulation of key transcriptional networks associated with angiogenesis. Independent mechanisms to chemically or genetically inhibit axonal ingrowth led to similar deficits in HO site angiogenesis, a reduction in type H vessels, and heterotopic bone formation. Finally, a combination of single cell transcriptomic approaches within the dorsal root ganglia identified key neural-derived angiogenic paracrine factors that may mediate neuron-to-vascular signaling in HO. These data provide further understanding of nerve-to-vessel crosstalk in traumatized soft tissues, which may reflect a key determinant of mesenchymal progenitor cell fate after injury.

关键词： aberrant trauma nerves

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Gastric per-oral endoscopic myotomy: Current status and future directions

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World Journal of Gastroenterology 2019年第21期25卷 2581-2590页

作者： Alexander Podboy Joo Ha hwang Linda A Nguyen Patricia Garcia thomas A Zikos Afrin Kamal George Triadafilopoulos John O Clarke Division of Gastroenterology and Hepatology Stanford University School of Medicine

Gastroparesis, or symptomatic delayed gastric emptying in the absence of mechanical obstruction, is a challenging and increasingly identified syndrome. Medical options are limited and the only medication approved by the Food and Drug Administration for treatment of gastroparesis is metoclopramide, although other agents are frequently used off label. With this caveat, first-line treatments for gastroparesis include dietary modifications, antiemetics and promotility agents, although these therapies are limited by suboptimal efficacy and significant medication side effects. Treatment of patients that fail first-line treatments represents a significant therapeutic challenge. Recent advances in endoscopic techniques have led to the development of a promising novel endoscopic therapy for gastroparesis via endoscopic pyloromyotomy, also referred to as gastric per-oral endoscopic myotomy or per-oral endoscopic pyloromyotomy. The aim of this article is to review the technical aspects of the per-oral endoscopic myotomy procedure for the treatment of gastroparesis, provide an overview of the currently published literature, and outline potential next directions for the field.

关键词： Gastroparesis Gastric per-oral endoscopic myotomy Per-oral endoscopic pyloromyotomy Myotomy Endoscopic myotomy Peroral endoscopic myotomy Gastric emptying

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<i>Sulfotransferase</i>1A1 G638A polymorphism, cigarette smoking and bladder cancer risk in Taiwan

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Advances in Bioscience and Biotechnology 2012年第3期3卷 186-190页

作者： Kun-Hung Shen Chia-Chang Wu Yuan-Hung Wang Steven K. Huang thomas I. S. hwang Chung-Hsin Yeh Guang-Dar Juang Department of Urology Chi-Mei Medical Center Tainan Chinese Taipei Department of Urology Shin-Kong Wu Ho-Su Memorial Hospital Taipei Chinese Taipei Department of Urology Shuang Ho Hospital Taipei Medical University Taipei Chinese Taipei Division of Urology Department of Surgery Shuang Ho Hospital Taipei Medical University Taipei Chinese Taipei

Cigarette smoking is a major risk factor for bladder cancer (BC). Sulfotransferase 1A1 (SULT1A1), a phase II enzyme, plays an important role in the metabolism of several carcinogens contained in cigarettes. The aim of this study was to investigate the relationship between SULT1A1 G638A polymorphism, cigarette smoking and bladder cancer risk in Taiwan. A total of 150 BC patients and 150 cancer-free controls were recruited from February 2002 to February 2009. Genotyping of the SULT1A1 G638A polymerphism was determined using the polymerase chain reaction-restricted fragment length polymorphism (PCR- RFLP) method. The odds ratio (OR) and 95% confidence interval (CI) were calculated as a measure of the combined effect of cigarette smoking and the SULT1A1 G638A polymorphism on BC risk. In the present study, we found that study subjects with the G/G genotype of the SULT1A1 gene had a significantly higher BC risk of 1.7 (95% CI = 1.3 - 3.2) compared with those carrying the combination of G/A and A/A genotypes. Moreover, ever smokers who carried the G/G genotype of the SULT1A1 gene had a significantly increased UC risk of 3.5 (95% CI = 2.5 - 10.2) compared with never smokers who carried the G/A and A/A genotypes as the reference group. In conclusion, our findings suggest that SULT1A1 G638A polymorphism is associated with the development of BC, especially among cigarette smokers.

关键词： Bladder Cancer Cigarette Smoking Polymorphism SULT1A1

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美韩两国在城市更新改造和住房供给中的公正性问题研究

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科技与法律 2008年第6期 80-84页

作者： June Manning thomas Hee-Yun hwang 王晓晓马强强美国密歇根大学韩国忠北国立大学美国伊利诺理工大学(IIT) 合肥师范学院物理与电子工程系

城市的更新和改造是现代城市规划的一个重要组成部分,这其过程中的公正性也是各国公众普遍关注的问题。本文以美国和韩国为例,回顾了这两个国家在城市更新和改造的历史进程,以及它们在制订政策用以解决和改善在诸如暴力拆迁和低收入群... 详细信息

城市的更新和改造是现代城市规划的一个重要组成部分,这其过程中的公正性也是各国公众普遍关注的问题。本文以美国和韩国为例,回顾了这两个国家在城市更新和改造的历史进程,以及它们在制订政策用以解决和改善在诸如暴力拆迁和低收入群体住房短缺等方面的问题,并指出美韩两国在缺乏强制性行为、补偿定义的狭义化、决策及公正性等方面仍然面临诸多挑战。

关键词：住房政策韩国社会公正性城市更新和改造城市政策

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放射诊断专业中人种、西班牙裔和性别差异的现状研究

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国际医学放射学杂志 2014年第2期 166-167页

作者： C.H.Chapman W.T.hwang S.Both C.R.thomas Jr C.Deville 蔡兆诚 Department of Radiation Oncology University of Pennsylvania Perelman School of Medicine3400 Civic Center Blvd TRC 2W Philadelphia PA 19104

目的评价在医学生有效培养这一流水线背景下美国放射诊断医生队伍中人种、西班牙裔和性别的差异性。材料与方法本研究原始数据来自公开的注册资源,不涉及

关键词：放射诊断女性流水线流水生产线生产线住院医师实习医师西班牙南欧人种种族

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Transporting Mammalian Cells at Ambient Temperature:A Viable Alternative to Dry Ice

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Advances in Bioscience and Biotechnology 2017年第4期8卷 127-133页

作者： Steingrimur Stefansson Seunghyun Han Ye In Jeon David S.Chung Peter hwang Huyen Le John L.Warden David Ho HeMemics Biotechnologies Inc RockvilleUSA Johns Hopkins University BaltimoreUSA George Washington University WashingtonDCUSA Division of Biology and Medicine Brown UniversityProvidenceUSA thomas Jefferson High School for Science and Technology AlexandriaUSA Nauah Solutions McleanUSA

The most common method of shipping cells between institutes and companies is sending them frozen, usually treated with anti-freeze solution (most commonly DMSO because it is less toxic than many alternatives), and then packaging them in dry ice for shipment. However many countries place restrictions on dry ice shipments. An alternative to shipping frozen cell vials is to send flasks of growing cells in media. This also has problems because cells in media have limited viability and the flasks can leak. Here we report on an alternative method for shipping viable cells at ambient temperature without dry ice or in media filled flasks. In this study we report on the development and properties of HemSol?. This is an inexpensive, eco-friendly and protects cell integrity at ambient temperature while maintaining viability. We have previously shown that HemSol? protects platelet and RBC function in cold storage and circulating tumor cells up to 6 days. Therefore we wanted to know if HemSol? could also be used to transport live cells. Since HemSol? is a liquid, we experimented with encasing the cells with HemSol? and gelatin so as to prevent dry ice shipment of cells and circumvent the shipping of cells in media. We performed mock shipping experiments where cells were stored in HemSol? gel kept at room temperature on a lab benchtop and cells stored in dry ice was also kept on lab benchtop for up to 2 days. After the mock shipping period, we analyzed cells for their functions. Our results show that cells in HemSol? gel have greater than 95% viability and restored biological functions in 2 hours, whereas, cells shipped in dry ice required more than 24 hours to recover and needed media change to remove the DMSO.

关键词： HemSol^(TM) HemSol^(TM) Gel Transportation Short Term Storage Cells Shipment Dry Ice Ambient Temperature Shipment

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中国台湾地区汉族青少年高血压3阶段结合多位点测试和基因表达分析的全基因组关联研究

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中华高血压杂志 2014年第12期22卷 1198-1198页

作者： Chiang KM Yang HC Liang YJ Chen JW hwang SM Ho HY Ting CT Lin TH Sheu SH Tsai WC Chen JH Leu HB Yin WH Chiu TY Chen CI Lin SJ thomas GN Tomlinson B Guo Y Gui HS Sham PC Lam TH Pan WH 练桂丽叶鹏

虽然已经进行了多次大规模的全基因组关联研究（genome—wide association studies，GWAs），但是仅少数研究成功地鉴定出能重复的、重要的高血压基因位点，而且在中国人中进行的相关研究更少。与迟发性高血压相比，青少年高血压（young... 详细信息

虽然已经进行了多次大规模的全基因组关联研究（genome—wide association studies，GWAs），但是仅少数研究成功地鉴定出能重复的、重要的高血压基因位点，而且在中国人中进行的相关研究更少。与迟发性高血压相比，青少年高血压（young—onset hypertension，YOH）被认为是更有希望治疗的疾病，因为其受到遗传因素的影响更大。

关键词：全基因组高血压基因表达分析青少年台湾地区 hypertension 中国测试

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Microwave Synthesis of Silver Nanoparticles Using Different Pentose Carbohydrates as Reducing Agents

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Journal of Chemistry and Chemical Engineering 2018年第1期12卷 1-10页

作者： David S. Chung Hanna Kim Jonathan Ko Justine Lee Bryant hwang Sohyun Chang ByungJun Kim Sung-Jae Chung Division of Biology and Medicine Brown University Providence R102912 USA Granada Hill Charter High School Granada Hills CA 91344 USA thomas Jefferson High School for Science and Technology Alexandria VA 22312 USA Langley High School Mclean VA 2210 USA Department of Biology School of Arts & Sciences Johns Hopkins University Baltimore MD 21218 USA Biological Sciences Carnegie Mellon University Pittsburgh PA 15213 USA Marymount University School of Arts and Sciences 2807 N. Glebe Road Arlington VA 22302 USA

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