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Comparative Transcriptional Profiling and Preliminary Study on Heterosis Mechanism of Super-Hybrid Rice

Molecular Plant 2010年第6期3卷 1012-1025页

作者： Gui-Sheng Song Hong-Li Zhai Yong-Gang Peng Lei Zhang Gang Wei Xiao- Ying Chen yu-Guo Xiao Lili Wang yue-Jun Chen Bin Wu Bin Chen yu Zhang Hua Chen Xiu-Jing Feng Wan-Kui Gong Yao Liu Zhi-Jie Yin Feng Wang Guo-Zhen Liu Hong-Lin Xu Xiao-Li Wei Xiao-Ling Zhao Pieter B.F. Ouwerkerk thomas Hankemeier Theo Reijmers Rob van der Heijden Cong-Ming Lu Mei Wang Jan van der Greef Zhen Zhu State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China National Plant Gene Research Center (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China State Key Laboratory of Genetic Engineering Institute of Genetics School of Life Sciences Fudan University Shanghai 200433 China Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China Fujian Province Key Laboratory of Genetic Engineering for Agriculture Fujian Academy of Agricultural Sciences Fuzhou 350003 China Beijing Institute of Genomics Chinese Academy of Sciences Beijing 101300 China Institute of Biology Leiden University Sylvius Laboratory Wassenaarseweg 72 2333 BE Leiden The Netherlands Leiden/Amsterdam Center for Drug Research Center for Medical Systems Biology Leiden University Einsteinweg 5 2500 RA Leiden The Netherlands SU BioMedicine and TNO Quality of Life Utrechtseweg 48 3700 AJ Zeist The Netherlands

Heterosis is a biological phenomenon whereby the offspring from two parents show improved and superior performance than either inbred parental lines. Hybrid rice is one of the most successful apotheoses in crops utilizing heterosis. Transcriptional profiling of F1 super-hybrid rice Liangyou-2186 and its parents by serial analysis of gene expression （SAGE） revealed 1183 differentially expressed genes （DGs）, among which DGs were found significantly enriched in pathways such as photosynthesis and carbon-fixation, and most of the key genes involved in the carbon-fixation pathway exhibited up-regulated expression in F1 hybrid rice. Moreover, increased catabolic activity of corresponding enzymes and photosynthetic efficiency were also detected, which combined to indicate that carbon fixation is enhanced in F1 hybrid, and might probably be associated with the yield vigor and heterosis in super-hybrid rice. By correlating DGs with yield-related quantitative trait loci （QTL）, a potential relationship between differential gene expression and phenotypic changes was also found. In addition, a regulatory network involving circadian-rhythms and light signaling pathways was also found, as previously reported in Arabidopsis, which suggest that such a network might also be related with heterosis in hybrid rice. Altogether, the present study provides another view for understanding the molecular mechanism underlying heterosis in rice.

关键词： Heterosis super-hybrid rice transcriptional profiling photosynthesis carbon fixation regulatory network.

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金属注射成型生物医用Ti-22Nb合金的表面形貌和细胞相容性（英文）

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Transactions of Nonferrous Metals Society of China 2018年第7期28卷 1342-1350页

作者：赵大鹏陈钰凯常可可 thomas EBEL Bérengère JCLUTHRIGNER-FEYERABEND Regine WILLUMEIT-R?MER Florian PYCZAK 湖南大学生物学院 Institute of Materials Research Helmholtz-Zentrum Geesthacht 中国科学院宁波材料技术与工程研究所

采用金属注射成型(MIM)工艺制备Ti-22Nb(质量分数,%)和商业纯钛(CP-Ti)参比样,并选取电弧熔炼后抛光的Ti-22Nb作为另一种参比样。采用激光共聚焦显微镜、扫描电子显微镜和酶联免疫检测仪检测每组样品的表面拓扑结构和细胞相容性。结果显... 详细信息

采用金属注射成型(MIM)工艺制备Ti-22Nb(质量分数,%)和商业纯钛(CP-Ti)参比样,并选取电弧熔炼后抛光的Ti-22Nb作为另一种参比样。采用激光共聚焦显微镜、扫描电子显微镜和酶联免疫检测仪检测每组样品的表面拓扑结构和细胞相容性。结果显示,MIM工艺可以得到微米级粗糙度,而且采用混合元素粉而不是单一钛粉作为原料可以获得更高的表面粗糙度和比表面积。所有测试样品都不存在细胞毒性。人原代细胞在MIM Ti-22Nb样品表面(特别是在闭孔附近)的吸附效果更好。

关键词：金属注射成型 Ti-Nb合金表面拓扑结构细胞吸附细胞增殖

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Hidden dynamics in the unfolding of individual membrane proteins

Hidden dynamics in the unfolding of individual membrane prot...

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第十届全国化学生物学学术会议

作者： Hao yu Matthew G.W.Siewny Devin T.Edwards Lyle Uyetake thomas T.Perkins School of Physics Huazhong University of Science and Technology JILA National Institute of Standards and Technology and University of Colorado Department of Molecular Cellular and Developmental Biology University of Colorado

Protein folding occurs as a set of transitions between structural states within an energy landscape. An oversimplified view of the folding process emerges when transiently populated states are undetected because of limited instrumental *** force spectroscopy optimized for 1-μs resolution, we reexamined the unfolding of individual bacteriorhodopsin（bR） molecules in native lipid bilayers. The experimental data reveal the unfolding pathway in unprecedented detail. Numerous newly detected intermediates — many separated by as few as 2– 3 amino acids —exhibited complex dynamics, including frequent refolding and state occupancies of＜10 μs. Equilibrium measurements between such states enabled the folding free-energy landscape to be deduced. These results sharpen the picture of the mechanical unfolding of membrane proteins and, more broadly, enable experimental access to previously obscured protein dynamics. [1]To facilitate these studies, we further developed a site-specific bioconjugation protocol between the force probe and membrane proteins. By labeling bR at its c-terminal with a copper-free click chemistry reagent, we gently but covalently coupled bR to an azide-derivatized, PEG-coated tip. Rapid and reversible dynamics was revealed in the very initial unfolding of bR, which was previously undetectable due to non-specific tip-sample adhesion. Interestingly, a new obligate intermediate state was discovered at lysine 216, where bR’s photoactive retinal binds to the *** state was mechanically robust: it withstood 150 pN for ＞60 s, suggesting a role for retinal in its stabilization. To test this hypothesis, we then performed dynamic force spectroscopy on bR with and without retinal. As expected, the state showed a significant reduction in its rupture force while all other major states showed no change. Equilibrium measurements of the rapid dynamics in the initial unfolding of bR will enable us to quantify membrane protein energetics under their most n

关键词： membrane protein folding single molecule force spectroscopy AFM

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弹性链型悬挂高速接触网参数的选取研究

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铁道工程学报 2009年第8期26卷 82-87页

作者：李文豪崔校玉陈维荣 thomas Reichmann 铁道第三勘察设计院集团有限公司天津300251 西南交通大学成都610031 西门子公司交通技术集团德国爱尔兰根91052

研究目的：为研究弹性链型悬挂高速接触网参数对受电弓／接触网系统动态性能的影响，采用三维接触网模型和质量-阻尼-刚度受电弓模型，对时速350km弹性链型悬挂高速接触网／受电弓系统进行仿真计算和分析。考虑到接触压力和运行的安全... 详细信息

研究目的：为研究弹性链型悬挂高速接触网参数对受电弓／接触网系统动态性能的影响，采用三维接触网模型和质量-阻尼-刚度受电弓模型，对时速350km弹性链型悬挂高速接触网／受电弓系统进行仿真计算和分析。考虑到接触压力和运行的安全性，对不同的动态仿真结果进行比较，据此选取合适的高速接触网参数。研究结论：通过研究表明，应优先采用120mm^2。镁铜合金接触线，张力不宜小于27kN；承力索采用120mm^2铜合金绞线，张力约为23kN；跨距取55～60m；结构高度取1．4～1．6m；弹性吊索张力不小于3．5kN；弹性吊索长度取16—18m；支柱吊弦间距约为4．5m；接触线坡度应控制为0；尽量避免波长为吊弦间隔2～6倍的接触线不平顺。

关键词：接触网参数弓网相互作用仿真接触压力抬升量

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Females choose gentle, but not healthy or macho males in Campbell dwarf hamsters （Phodopus campbelli thomas 1905）

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Current Zoology 2017年第5期63卷 545-554页

作者： Konstantin A. ROGOVIN Anastasiya M. KHRUSHCHOVA Olga N. SHEKAROVA Nina A. VASlLIEVA Nina yu VASILIEVA A.N. Severtsov Institute of Ecology and EvoLution RAS Leninskii prospect 33 Moscow 119071 Russia

Androgen-dependent male sexual traits （STs） as well as immunocompetence are theoreticallyassumed to be key indicators of a male＇s quality for the mate-choosing female. We studied matechoice by sexually motivated （SM） females of Campbell＇s dwarf hamsters. Females chose between2 tethered male siblings that differed in expression of STs. Males were unrelated to the female andable to contact and copulate with her. In both males, we measured sex-related morphology ofbody mass, mid-ventral specific skin gland, ano-genital distance, and external testicular *** also estimated levels of blood testosterone and cortisol, specific T- and B-cell immune re-sponses to antigens, as well as aggressive and sexual dominance in sibling males through add-itional encounter experiments with another SM female （male sibs could freely compete for thefemale）. We found that SM females chose a partner among 2 male sibs and spent over 80% of theirtime on average with the preferred male compared with the non-preferred one. Her choice was notassociated with the first visit of the chosen male, with a higher expression of sex-related traits,higher levels of blood testosterone, or with aggressive dominance. The choice was not associatedwith the intensity of T-cell immune response to phitohemagglutinin （PHA）. Instead there was a ten-dency for a negative relationship with the expression of STs and B-cell response to the antigenchallenge. The only character that unambiguously influenced female choice was the non-aggressive male to female grooming during sexual contact. There was no difference in breedingsuccess between preferred and non-preferred males paired with virgin females.

关键词： Campbell's dwarf hamsters, female mate choice, immunocompetence handicap, male-male aggression, sexual traits,testosterone.

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PRDM蛋白家族在神经系统中的作用

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生理科学进展 2020年第2期51卷 87-93页

作者：王存金潘昱辰陈莹庄英涵 thomas Behnisch 复旦大学脑科学研究院医学神经生物学国家重点实验室脑科学协同创新中心上海200032 武警江苏总队医院内三科扬州225003

PRDM(PRDI-BF1 and RIZ homology domain containing)蛋白家族是一类包含PR结构域和若干个锌指结构的转录调控蛋白,具有众多家庭成员,广泛表达于灵长类、两栖类和啮齿类等动物中。以往研究发现,PRDM蛋白家族(PRDMs)在细胞增殖、分化、... 详细信息

PRDM(PRDI-BF1 and RIZ homology domain containing)蛋白家族是一类包含PR结构域和若干个锌指结构的转录调控蛋白,具有众多家庭成员,广泛表达于灵长类、两栖类和啮齿类等动物中。以往研究发现,PRDM蛋白家族(PRDMs)在细胞增殖、分化、成熟过程中,以及机体造血、生殖和肿瘤发生等生理和病理过程中发挥重要的调控作用。近年来随着研究的不断深入,有关PRDMs在神经系统中所发挥的作用也逐渐引起科学界的广泛关注。目前的研究证实了PRDMs在神经系统发育、中枢神经系统炎症以及神经干细胞增殖和分化等过程中扮演着重要的角色。本文就PRDMs在神经系统中的作用作一综述。

关键词： PRDM 神经系统表观遗传信号通路

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澳洲杨(大戟科)胚珠及其种子附属结构的个体发育研究

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西北植物学报 2015年第2期35卷 289-293页

作者：张鑫 thomas Stützel 张志翔于海燕王利兵西北农林科技大学林学院陕西杨陵712100 波鸿鲁尔大学植物多样性与进化系生物科学与技术学院德国波鸿44780 北京林业大学自然保护区学院北京100083 中国林业科学研究院林业研究所北京100091

以澳洲杨的胚珠及其种子为材料,运用光学显微镜和电镜扫描的方法对其从胚珠发生直到种子成熟的个体发育过程和结构进行观察,同时与鸭脚西番莲的种子附属结构的发育过程进行对比研究。结果表明:(1)澳洲杨的珠孔类型为外珠孔类型,种子附... 详细信息

以澳洲杨的胚珠及其种子为材料,运用光学显微镜和电镜扫描的方法对其从胚珠发生直到种子成熟的个体发育过程和结构进行观察,同时与鸭脚西番莲的种子附属结构的发育过程进行对比研究。结果表明:(1)澳洲杨的珠孔类型为外珠孔类型,种子附属结构起源于珠孔而非珠柄,其为种阜,而非假种皮。(2)鸭脚西番莲的珠孔类型为内珠孔类型,种子附属结构起源于珠柄,并且最终将珠孔包被,其为真正的假种皮结构。通过种阜与假种皮的不同个体发育过程,建立了大戟科种阜与假种皮的不同发育模式,并对种子附属结构的生物学功能及其暗示的不同植物进化路径进行了讨论。

关键词：种子附属物假种皮种阜澳洲杨大戟科西番莲属

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Obligation-based Theory ***-based Theory:Confrontation of Ideas Between Warrender and Strauss

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The Journal of Human Rights 2023年第5期22卷 1134-1150页

作者：唐学亮 JIANG yu(译) the Xi’an Jiaotong University School of Law 不详

There is a transition from the objective laws or moral orders that precede human will in classical natural law to the subjective demands or rights emanating from human will in modern natural law,and it represents a historical debate on the shift from an obligation-based theory to a right-based ***,within the context of this transition across time,assesses thomas Hobbes's philosophy of law and recognizes him as the founder of modern natural rights *** Wesley Newcomb Hohfeld's analysis of jurisprudence,Howard Warrender assesses the privilege nature of Hobbes's concept of natural rights and concludes that,since Hohfeld's privilege is the opposite of obligation and related to no-right,obligations cannot be derived from natural ***,Warrender argues that Strauss's assertion requires ***,Warrender places excessive emphasis on Hohfeld's static separation of the concept of privilege within his theoretical system,overlooking the dynamic transformation from privilege to claim *** this regard,Hobbesian scholar Carlan's criticism of Warrender is ***,Warrender's research holds theoretical significance in that he,under the premise of being a part of Hobbes'natural law tradition,transforms Hohfeld's flat,two-party legal rights relationships into a three-party legal rights structure,which could represent a potential innovation in the 20^(th)century legal philosophy.

关键词： obligation nature rights privilege Warrender Strauss

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Inhibition of vascular endothelial growth factor expression by Chinese medicine of Hedyotis diffusa Willd herbal compounds

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Frontiers in Biology 2010年第4期5卷 361-368页

作者： Min WANG Zhumei SHI Dan LIU Gong-yu ZHANG Jiahao SHA Bing-Hua JIANG Laboratory of Reproductive Medicine Department of PathologyCancer CenterNanjing Medical UniversityNanjing 210029China School of Life Science Soochow UniversitySuzhou 215021China Nanjing University of Traditional Chinese Medicine Nanjing 210046China Department of Pathology Anatomy and Cell BiologyThomas Jefferson UniversityPhiladelphiaPA 19107USA

The Hedyotis diffusa Willd herbal compounds(HDWHCs)are commonly used as Chinese medicine to treat cancer patients with established clinical therapeutic efficacy in ***,the underlying mechanisms remain to be *** this study,we used freeze-dried powder of the water extracts of HDWHCs to investigate the potential mechanisms of HDWHCs in cancer *** treatment significantly inhibited vascular endothelial growth factor(VEGF)mRNA levels and VEGF transcriptional activation in cancer *** also had a remarkable inhibitory effect on the expression of hypoxia-inducible factor 1alpha(HIF-1alpha).Forced expression of HIF-1αrestored VEGF transcriptional activation inhibited by HDWHCs,indicating that HDWHCs suppressed VEGF expression through decreasing HIF-1alpha ***,HDWHCs inhibited cyclooxygenase-2(COX-2)expression,and overexpression of HIF-1alpha restored HDWHCs’inhibitory effect on COX-2 at transcriptional *** findings may provide better understanding of HDWHCs’anti-cancer mechanism in cancer treatment.

关键词： Hedyotis diffusa Willd Chinese medicine vascular endothelial growth factor(VEGF) hypoxiainducible factor 1alpha(HIF-1alpha) cyclooxygenase-2(COX-2)

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腰椎术后邻近节段退变及再手术

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中华骨科杂志 2018年第13期38卷 818-826页

作者：郎昭于研田伟 Louis G.Jenis thomas D.Cha 李国安北京积水潭医院脊柱外科 100035 上海同济医院脊柱外科 200065 Department of Spine Surgery Newton-Wellesley Hospital USA02459 Department of Spine Surgery Massachusetts General Hospital USA02114 Orthopaedic Bioengineering Research Center Department of Orthopaedic Surgery Harvard Medical School/Newton-Wellesley Hospital USA02459

腰椎邻近节段退变是指腰椎手术后手术节段头端或尾端发生的退行性改变，其中一部分患者同时出现相应的临床症状，称之为邻近节段疾病。近年来虽然有非常多的新技术涌现，但邻近节段退变并未真正得到解决。通过对MEDLINE／PubMed、Cochra... 详细信息

腰椎邻近节段退变是指腰椎手术后手术节段头端或尾端发生的退行性改变，其中一部分患者同时出现相应的临床症状，称之为邻近节段疾病。近年来虽然有非常多的新技术涌现，但邻近节段退变并未真正得到解决。通过对MEDLINE／PubMed、Cochrane Controlled Trials Registry、EMBASE数据库进行检索，纳入所有使用融合和（或）固定、单纯减压或人工腰椎间盘置换三种手术治疗腰椎退行性疾病的随机或非随机临床研究，同时保证纳入研究的病例数量〉t20例及患者年龄≥18岁。排除个案报道、综述或荟萃分析、无法获得摘要的文献，以及创伤、感染、肿瘤和炎症性疾病的研究。主要针对邻近节段退变的定义及诊断标准，以及邻近节段退变及再手术的发生率和危险因素进行综述。文献中邻近节段退变的概念在使用上较为混乱，影像学上退变同症状性退变常常混杂使用，因此对文献间进行横向比较得出结论较为困难。此外，既往研究并未全面了解邻近节段退变的发病机制及危险因素，特别是手术节段对邻近节段的影响。且目前文献报道的结果多为回顾性研究所得，尚缺乏高等级证据。腰椎融合术后再手术的主要原因是邻近节段退变，降低再手术率最主要的方法就是降低邻近节段退变的发生率。应首先统一邻近节段退变的诊断标准，同时对包括手术节段在内的各种囡素进行全面研究，通过高等级证据阐明邻近节段退变的发生机制和危险因素。

关键词：邻近节段退变术后再手术腰椎术后腰椎退行性疾病 Cochrane 人工腰椎间盘置换随机临床研究 MEDLINE

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