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Physiological Quality and Seed Production of Corn and Fabaceae in monoculture and Intercropping

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American Journal of Plant Sciences 2017年第11期8卷 2597-2607页

作者： Anna Christina Sanazário de Oliveira Fábio cunha Coelho Henrique Duarte Vieira Jocarla Ambrosim Crevelari Aurea Izabel Aguiar Fonseca e Souza Tiago massi Ferraz Antonia Alice Costa Rodrigues Programa de Pós-Graduacao em Agroecologia Centro de Ciências AgráriasUniversidade Estadual do MaranhaoSao LuísBrasil Laboratório de Fitotecnia Campos dos GoytacazesUniversidade do Norte Fluminense Darcy RibeiroCamposBrasil Laboratório de melhoramento Genético Vegetal Campos dos GoytacazesUniversidade do Norte Fluminense Darcy RibeiroCamposBrasil Universidade Federal Rural da Amazonia BelémBrasil

The aim of this study was to evaluate the physiological quality and seed production of Fabaceae and corn in monoculture and intercropping in areas cropped under different management. The following treatments were evaluated: corn + NPK + weed control;corn + weed control;corn + NPK;corn + crotalaria;corn + jack bean. Intercropping raised the average number of corn ears of corn and the seed IVG Weed control positively influenced the final stand and average number of ears. NPK fertilization on corn sowing positively influenced the seed electrical conductivity. The yield of corn plants intercropped with crotalaria and jack bean was statistically similar. However, their absolute values should be considered for economic purposes. Corn seeds derived from intercropping with crotalaria were more effective compared with intercropping with jack bean. Crotalaria and jack bean seeds when intercropped with corn have high production, germination and vigor (IVG and emergence).

关键词： Zea mays Green manure No-Till System Vigor Germination

Searching for nuclear export elements in hepatitis D virus RNA

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World Journal of Virology 2013年第3期2卷 123-135页

作者： Natália Freitas Celso cunha Department of microbiology Molecular Genetics and Immunology Kansas University Medical Center Rainbow Boulevard Kansa KS 66160 United States medical microbiology Unit Center for Malaria and Tropical Diseases Institute of Hygiene and Tropical Medicine Nova University of Lisbon 1349-008 Lisbon Portugal

AIm: To search for the presence of cis elements in hepatitis D virus(HDV) genomic and antigenomic RNA capable of promoting nuclear export. mETHODS: We made use of a well characterized chloramphenicol acetyl-transferase reporter system based on plasmid p Dm138. Twenty c DNA fragments corresponding to different HDV genomic and antigenomic RNA sequences were inserted in plasmid pD m138, and used in transfection experiments in Huh7 cells. The relative amounts of HDV RNA in nuclear and cytoplasmic fractions were then determined by realtime polymerase chain reaction and Northern blotting. The secondary structure of the RNA sequences that displayed nuclear export ability was further predicted using a web interface. Finally, the sensitivity to leptomycin B was assessed in order to investigate possible cellular pathways involved in HDV RNA nuclear ***: Analysis of genomic RNA sequences did not allow identifying an unequivocal nuclear export element. However, two regions were found to promote the export of reporter m RNAs with efficiency higher than the negative controls albeit lower than the positive control. These regions correspond to nucleotides 266-489 and 584-920, respectively. In addition, when analyzing antigenomic RNA sequences a nuclear export element was found in positions 214-417. Export mediated by the nuclear export element of HDV antigenomic RNA is sensitive to leptomycin B suggesting a possible role of CRm1 in this transport pathway. CONCLUSION: A cis-acting nuclear export element is present in nucleotides 214-417 of HDV antigenomic RNA.

关键词： Hepatitis D virus Genomic RNA Antigenomic RNA Nuclear export Nuclear export element

Real-world disease activity and sociodemographic,clinical and treatment characteristics of moderate-to-severe inflammatory bowel disease in Brazil

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World Journal of Gastroenterology 2021年第2期27卷 208-223页

作者： Cyrla Zaltman Rogério Serafim Parra Ligia Yukie Sassaki Genoile Oliveira Santana maria de Lourdes Abreu Ferrari Sender J miszputen Heda m B S Amarante Roberto Luiz Kaiser Junior Cristina Flores Wilson R Catapani Josémiguel Luz Parente mauro Bafutto Odery Ramos Carolina D Gonçalves Isabella miranda Guimaraes Jose J R da Rocha marley R Feitosa Omar Feres Rogerio Saad-Hossne Francisco Guilherme Cancela Penna Pedro Ferrari Sales cunha Tarcia NF Gomes Rodrigo Bremer Nones mikaell Alexandre Gouvea Faria mírian Perpétua Palha Dias Parente António S Scotton Rosana Fusaro Caratin Juliana Senra Júlio maria Chebli Department of Internal medicine Federal University of Rio de JaneiroRio de Janeiro 21941-913Brazil Department of Surgery and Anatomy Ribeirão Preto Medical SchoolUniversity of São PauloRibeirao Preto 14049-900São PauloBrazil Department of Internal medicine São Paulo State University(UNESP)Medical SchoolBotucatu 18618-687São PauloBrazil IBD Unit Federal University of BahiaSalvador 41150-000BahiaBrazil Department of Clinical medicine Medical SchoolUniversidade Federal de Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil Department of Gastroenterology Escola Paulista de MedicinaSão Paulo 04023-900Brazil Hospital de Clinicas Universidade Federal do ParanaCuritiba 80060-900ParanaBrazil Department of Proctology Beneficencia Portuguesa Hospital/Kaiser Day HospitalSao Jose do Rio Preto 15015110São PauloBrazil Department of Gastroenterology and Hepatology Sciences Hospital de ClínicasUniversidade Federal do Rio Grande do SulPorto Alegre 90560002Rio Grande do SulBrazil Department of Gastroenterology Faculdade de Medicina do ABCSanto Andre 09060-870São PauloBrazil Department of General medicine Gastroenterology UnitUniversity HospitalFederal University of PiauiTeresina 64049-550PiauíBrazil Department of Gastroenterology Faculdade de MedicinaUniversidade Federal de GoiásGoiania 74535-170GoiasBrazil Department of Surgery Botucatu Medical School at São Paulo State University(UNESP)Botucatu 18618687São PauloBrazil Department of Gastroenterology UNIFESPSão Paulo 04040-002Brazil IBD unit Gastroenterology DepartmentHospital Nossa Senhora das GraçasCuritiba 80810-040ParanaBrazil Department of Proctology Kaiser Hospital DiaSao Jose do Rio Preto 15015-110São PauloBrazil Health Sciences Center Epidemiology UnitState University of PiauiTeresina 64001-280PiauíBrazil Department of Gastroenterology CMIP Centro Mineiro de PesquisaJuiz de Fora 36010-570Minas GeraisBrazil Scientific Affairs Takeda Pharmaceuticals BrazilSão Paulo 04709-011Brazil Clinical Research Takeda Pharma

BACKGROUND Understanding the treatment landscape of inflammatory bowel diseases(IBD)is essential for improving disease management and patient *** is the largest Latin American country,and it presents socioeconomic and health care differences across its geographical *** country has the highest increase in IBD incidence and prevalence in Latin America,but information about the clinical and treatment characteristics of IBD is *** To describe the sociodemographic,clinical,and treatment characteristics of IBD outpatients in Brazil overall and in the Southeast,South and Northeast/midwest *** multicenter,cross-sectional study with a 3-year retrospective chart review *** with moderate-to-severe Crohn’s disease(CD)or ulcerative colitis(UC)were consecutively enrolled between October 2016 and February *** CD at enrollment was defined as a Harvey Bradshaw Index≥8 or a CD Activity Index≥220 or a calprotectin level>200μg/g or an active result based on colonoscopy suggestive of inadequate control during the previous year;active UC was defined as a partial mayo score≥*** statistics were used to analyze all *** In a total of 407 included patients,CD was more frequent than UC,both overall(264 CD/143 UC patients)and by region(CD:UC ratios of 2.1 in the Southeast,1.6 in the South and 1.2 in the Northeast/midwest).The majority of patients were female(54.2%of CD;56.6%of UC),and the mean ages were 45.9±13.8 years(CD)and 42.9±13.0 years(UC).The median disease duration was 10.0(range:0.5-45)years for both IBD *** enrollment,44.7%[95%confidence interval(CI):38.7-50.7]of CD patients and 25.2%(95%CI:18.1-32.3)of UC patients presented with active *** than 95%of IBD patients were receiving treatment at enrollment;CD patients were commonly treated with biologics(71.6%)and immunosuppressors(67.4%),and UC patients were commonly treated with mesalazine[5-Aminosalicylic acid(5-ASA)]derivates(69.9%)and

关键词： Inflammatory bowel disease Crohn's disease Ulcerative colitis Disease activity Epidemiology Treatment

Comparison of Three Experimental models for Rat Osteoarthritis Induction

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Journal of Biosciences and medicines 2016年第12期4卷 62-69页

作者： Henrique Ribeiro Rodrigues-Neto Edilson Ferreira Andrade-Junior Denilson Jose Silva Feitosa-Junior André Lopes Valente thiago Cesar Xavier Bianca Caroline do Nascimento Alho Renan Kleber Costa Teixeira Fabio Vidal moriya Rui Sérgio monteiro de Barros Department of Orthopaedics and Traumatology Porto Dias Hospital Bel&eacute m Brazil Experimental Surgery Laboratory Par&aacute State University Bel&eacute m Brazil

Background: Osteoarthritis is a slowly progressive and debilitating disease with high prevalence in adult population. Knee is one of the joints most affected by this disorder. There are several models for animals’ osteoarthritis induction, however it is not identified any paper that compares these techniques. The present study was aimed to define the most appropriate model for rats osteoarthritis induction. material and methods: 40 Wistar rats were distributed into 4 groups of 10 animals each: normality group (NG);meniscectomy group (mG);quinolone group (QG) and iodoacetate group (IG). Radiographic images of the rat’s knees were analyzed as well as the amount of chondrocytes in the epiphyseal and articular cartilage. Results: In the radiographic analysis, there was a low correlation between the raters. Regarding the amount of chondrocytes in the epiphyseal cartilage, it was noticed that the IG and QG groups had fewer chondrocytes than NG, in contrast to mG that reported similar results to normality (p > 0.05). There was no significant difference between IG and QG groups (p > 0.05). Regarding the amount of chondrocytes in articular cartilage, it was noticed that the IG group showed fewer chondrocytes than NG (p 0.05). There was no significant difference between QG and mG groups (p > 0.05). Conclusion: Intraarticular injection of iodoacetate in rats is the model with greatest effect on reduction of chondrocytes amount.

关键词： Osteoarthritis Knee Joint Experimental Arthritis Animal Disease models Rats

Effects of intragastric balloon placement in metabolic dysfunctionassociated fatty liver disease: A systematic review and metaanalysis

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World Journal of Hepatology 2021年第7期13卷 815-829页

作者： João Remíde Freitas Júnior Igor Braga Ribeiro Diogo Turiani Hourneaux de moura Vitor massaro Takamatsu Sagae Gabriel mayo Vieira de Souza Guilherme Henrique Peixoto de Oliveira Sergio A Sánchez-Luna thiago Ferreira de Souza Eduardo Turiani Hourneaux de moura Cláudia Pinto marques Souza de Oliveira Wanderley marques Bernardo Eduardo Guimarães Hourneaux de moura Serviço de Endoscopia Gastrointestinal do Hospital das Clínicas HCFmUSP Departamento de GastroenterologiaFaculdade de MedicinaUniversidade de Sao PauloSao PauloSPBR Division of Gastroenterology and Hepatology Department of Internal MedicineBasil I.Hirschowitz Endoscopic Center of ExcellenceThe University of Alabama at BirminghamBirminghamAL 35294United States Department of Gastroenterology Hospital das Clínicas da Faculdade de Medicina da Universidade de São PauloSão Paulo 05403-010Brazil

BACKGROUND metabolic dysfunction-associated fatty liver disease corresponds to a clinical entity that affects liver function triggered by the accumulation of fat in the liver and is linked with metabolic *** To evaluate the effects of the intragastric balloon(IGB)in patients with metabolic dysfunction-associated fatty liver disease through the assessment of liver enzymes,imaging and several metabolic *** A comprehensive search was done of multiple electronic databases(mEDLINE,EmBASE,LILACS,Cochrane and Google Scholar)and grey literature from their inception until February *** criteria involved patients with a body mass index>25 kg/m2 with evidence or previous diagnosis of hepatic *** analyzed before and after 6 mo of IGB removal were alanine aminotransferase(IU/L),gamma-glutamyltransferase(IU/L),glycated hemoglobin(%),triglycerides(mg/dL),systolic blood pressure(mmHg),homeostatic model assessment,abdominal circumference(cm),body mass index(kg/m2)and liver volume(cm3).RESULTS Ten retrospective cohort studies evaluating a total of 508 patients were *** 6 mo of IGB placement,this significantly reduced alanine aminotransferase[mean difference(mD):10.2,95%confidence interval(CI):8.12-12.3],gammaglutamyltransferase(mD:9.41,95%CI:6.94-11.88),glycated hemoglobin(mD:0.17%,95%CI:0.03-0.31),triglycerides(mD:38.58,95%CI:26.65-50.51),systolic pressure(mD:7.27,95%CI:4.79-9.76),homeostatic model assessment(mD:2.23%,95%CI:1.41-3.04),abdominal circumference(mD:12.12,95%CI:9.82-14.41)and body mass index(mD:5.07,95%CI:4.21-5.94).CONCLUSION IGB placement showed significant efficacy in improving alanine aminotransferase and gamma-glutamyltransferase levels in patients with metabolic dysfunctionassociated fatty liver disease as well as improving metabolic markers related to disease progression.

关键词： Intragastric balloon metabolic dysfunction-associated fatty liver disease Homeostatic model assessment Abdominal circumference Body mass index

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巴西BCG卡痕阅读的敏感性与特异性

国际结核病与肺部疾病杂志 2002年第1期 35-37页

作者： S.m.Pereira I.Dourado m.L.Barreto S.S.cunha m.Y.T.Ichiara m.A.Hijjar J.C.L.Goes L.C.Rodrigues 安燕生 Instituto de Saude Coletiva Salvador Bahia Brazil Infectious Disease and Epidemiology Unit London School of Hygiene and Tropical Medicine London England Coordenaco Regional da Fundaco Nacional de Sade Salvador Bahia Centro de Referência Hélio Fraga/Cenepi/FUNASA Rio de Janeiro Brazil Instituto de Saúde Coletiva Salvador Bahia Brazil Infectious Disease and Epidemiology Unit London School of Hveiene and Tropical Medicine London England

在巴西社区的一个BCG效果评价试验的横断面调查中,培训过的人员检查儿童的上臂,同时从监护人的来信及儿童接种卡上获得信息。随后护士再对样本进行双盲抽查。两组卡痕阅读有高度的一致性(Kappa=0.839)。以监护人和卡片记录的信息作为金... 详细信息

在巴西社区的一个BCG效果评价试验的横断面调查中,培训过的人员检查儿童的上臂,同时从监护人的来信及儿童接种卡上获得信息。随后护士再对样本进行双盲抽查。两组卡痕阅读有高度的一致性(Kappa=0.839)。以监护人和卡片记录的信息作为金标准获得高敏感性和低特异性。当监护人与接种卡片一致时敏感性仍然高,而当两者不一致时,敏感性仍然高,而特异性非常低。(BCG)卡痕是已接种BCG的好指标。

关键词：确认敏感性特异性 BCG疫苗 BCG卡痕

Phytochemical bioprospecting, antioxidant, antimicrobial and cytotoxicity activities of saline extract from Tithonia diversifolia(Hemsl) *** leaves

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Asian Pacific Journal of Tropical Biomedicine 2018年第5期8卷 245-253页

作者： Bruno Rafael Barboza Bárbara Rafaela da Silva Barros Bárbara de Azevedo Ramos maiara Celine de moura thiago Henrique Napoleao maria Tereza dos Santos Correia Luana Cassandra Breitenbach Barroso Coelho Iranildo José da Cruz Filho Ana maria Souto maior Túlio Diego da Silva Leylianne de Cássia Rodrigues Nerys Edson Renan Barros de Santana Cláudia Sampaio de Andrade Lima Virgínia maria Barros de Lorena Cristiane moutinho Lagos de melo Department of Antibiotics Biosciences Centre Federal University of Pernambuco Department of Biochemistry Biosciences Centre Federal University of Pernambuco Center for Strategic Techmologies of the Northeast Department of Biophysics Biosciences Centre Federal University of Pernambuco Aggeu magalhaes Institute IAM/FIOCRUZ

Objective:To evaluate antimicrobial and antioxidant properties of saline extract from Tithonia diversifolia leaves by phytochemical bioprospecting,and investigate its safety against animal ***:The saline extract was prepared,with NaCl(0.15 m),by constant stirring of the dried and pulverized leaves,followed by volume reduction by *** extract was phytochemical characterized using ultra-performance liquid chromatography,and total phenol and flavonoid analysis also was *** antioxidant capacity was determined through DPPH*radical,the antimicrobial property was evaluated against standard bacteria and fungi,and the viability assays were performed against mice ***:Fifteen compounds were identified belonging to two main classes terpenoids and *** extract showed 22.185 mg GAE/g of total phenolic compounds and 3.220 mg QE/g of ***,extract showed higher antioxidant ability similar to butylated hydroxytoluene a standard molecule[(3.042±0.019)mg AAE/g and(4.12±0.10)mg AAE/g to saline extract and butylated hydroxytoluene,respectively].The antimicrobial assays demonstrated that the extract had a significant antifungal potential against Candida species and could be used with safety against mice splenocytes,in concentrations lower than 50μg/mL,promoting higher proliferation in these ***:Saline extract from Tithonia diversifolia leaves presents potential antioxidant,antifungal properties and induces immunostimulation in mice splenocytes.

关键词： Tithonia diversifolia Extract Antioxidant Antifungal properties Immunostimulation

Reduction of the Protective Action of Corrosion Inhibitors for Carbon Steel in Acidification by Using Hydrochloric Acid Contaminated with Chlorine

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材料科学与工程（中英文A版） 2016年第5期6卷 260-269页

作者： Femando Benedicto mainier Alan Eduardo R. de Freitas Anne Aparecida m. Figueiredo thiago Teobaldo Silva Engineering School Federal Fluminense University (UFF) Niteroi Rio de Janeiro 24220-261 Brazil

Diabetic neuropathic pain:Physiopathology and treatment

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World Journal of Diabetes 2015年第3期6卷 432-444页

作者： Anne K Schreiber Carina Fm Nones Renata C Reis Juliana G Chichorro Joice m.cunha Department of Pharmacology Biological Sciences SectorFederal University of Parana

Diabetic neuropathy is a common complication of both type 1 and type 2 diabetes,which affects over 90% of the diabetic *** pain is one of the main symptoms of diabetic neuropathy,its pathophysiological mechanisms are not yet fully *** is widely accepted that the toxic effects of hyperglycemia play an important role in the development of this complication,but several other hypotheses have been *** management of diabetic neuropathic pain consists basically in excluding other causes of painful peripheral neuropathy,improving glycemic control as a prophylactic therapy and using medications to alleviate *** line drugs for pain relief include anticonvulsants,such as pregabalin and gabapentin and antidepressants,especial y those that act to inhibit the reuptake of serotonin and *** addition,there is experimental and clinical evidence that opioids can be helpful in pain control,mainly if associated with first line *** agents,including for topical application,such as capsaicin cream and lidocaine patches,have also been proposed to be useful as adjuvants in the control of diabetic neuropathic pain,but the clinical evidence is insufficient to support their *** conclusion,a better understanding of the mechanisms underlying diabetic neuropathic pain will contribute to the search of new therapies,but also to the improvement of the guidelines to optimize pain control with the drugs currently available.

关键词： Diabetes Neuropathic pain Hyperglycemia Anticonvulsants Antidepressants