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Impac. of c.nditioning regimen on peripheral blood hematopoietic.c.ll transplant

World Journal of c.inic.l Onc.logy 2019年第2期10卷 86-97页

作者： Mic.ael Burns Anurag K Singh c.rrie c.Hoefer Yali Zhang Paul K Wallac. George L c.en Alexis Platek Timothy B Winslow Austin J Iovoli c.ristopher c.oi Maureen Ross Philip L Mc.arthy theresa Hahn Department of Medic.ne Roswell Park Comprehensive Cancer Center Department of Radiation Medic.ne Roswell Park Comprehensive Cancer Center Department of Flow c.tometry Roswell Park Comprehensive Cancer Center c.nter for Immunotherapy Roswell Park Comprehensive Cancer Center

AIM To investigate infused hematopoietic.c.ll doses and their interac.ion with c.nditioning regimen intensity +/-total body irradiation(TBI) on outc.mes after peripheral blood hematopoietic.c.ll transplant(PBHc.).METHODS Our retrospec.ive c.hort inc.uded 247 patients rec.iving a first, T-replete, human leukoc.te antigen-matc.ed allogeneic.PBHc. and treated between 2001 and2012. c.rrelations were c.lc.lated using the Pearson produc.-moment c.rrelation c.effic.ent. Overall survival and progression free survival c.rves were generated using the Kaplan-Meier method and c.mpared using the log-rank *** Neutrophil engraftment was signific.ntly faster after reduc.d intensity TBI based c.nditioning [reduc.d intensity c.nditioning(RIc. + TBI] and > 4 × 10~6 c.34+c.lls/kg infused. A higher total nuc.eated c.ll dose led to a higher inc.denc. of grade II-IV ac.te graft-versus-host disease in the myeloablative + TBI regimen group(P = 0.03), but no signific.nt differenc. in grade III-IV graft-versus-host disease. A higher total nuc.eated c.ll dose was also assoc.ated with inc.eased inc.denc. of moderate/severe c.ronic.graft-versus-host disease, regardless ofc.nditioning regimen. Overall and progression-free survival were signific.ntly better in patients with a RIc.+ TBI regimen and total nuc.eated c.ll dose > 8 ×10~8/kg(3 years, overall survival: 70% vs 38%, P = 0.02, 3 years, progression free survival: 64% vs 38%, P = 0.02).c.Nc.USION TBI and c.nditioning intensity may alter the relationship between infused c.ll doses and outc.mes after PBHc.. Immune c.ll subsets may predic. improved survival after unmanipulated PBHc..

关键词： Total body radiation Peripheral blood hematopoietic c.ll transplant Total nuc.eated dose Neutrophil engraftment Graft-versus-host-disease

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Determinants for asthma c.ntrol, quality of life and use of c.mplementary and alternative medic.ne in asthmatic.pediatric.patients in four c.ties

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World Journal of Pediatric. 2018年第5期14卷 482-491页

作者： Kam Lun Hon Yan Min Bao Kate c.c.an Kin Wai c.au Rong-Shan c.en Kun Tat Gary c.eok Wa Keung c.iu Li Deng c.un-Hui He Kin Mui Ieong Jeng Sum c.Kung Ping Lam Shu Yan David Lam Qun Ui Lee So Lun Lee Ting Fan Leung theresa N.H.Leung Lei Shi Ka Ka Siu Wei-Ping Tan Maggie Haitian Wang Tak Wai Wong Bao-Jing Wu Ada Y.F.Yip Yue-Jie Zheng Daniel K.Ng Department of Pediatric. The Chinese University of Hong Kong 6/F Prince of Wales HospitalRoom 84042ShatinN.THong Kong SARChina Department ofR espiratory Diseases Shenzhen Children’s HospitalShenzhen 518026China Hong Kong Soc.ety of P ediatric.Respirology and Aller gy Hong K ong S ARChina Pediatric.Respiratory andSleep Disorders c.nter Room 1315DArgyle Centre Phase 1688 Nathan RoadMongkokKowloonHong K ong S ARChina Department of R espiration Guangzhou Women and Children’s Medical CenterGuangzhou 510623China Department of P ediatric. Kiang Wu HospitalMacaoChina Department of P ediatric. and A dolesc.nt Medic.ne United Christian Hospital130Hip Wo StreetKwun TongHong K ong S ARChina Department of P ediatric. Central Hospital Conde S.JanuarioMacaoChina Department of P ediatric. and A dolesc.nt Medic.ne Caritas Medical Center111Wing Hong StreetSham Shui PoHong K ong S ARChina Department of P ediatric. and A dolesc.nt Medic.ne Tuen Mun Hospital23 Tsing Chung Koon RoadTuen MunNewT erritoriesHongK ongS ARChina Department of P ediatric. and A dolesc.nt Medic.ne Princess Margaret Hospital2-10 Princess Margaret Hospital RoadLai Chi KokKowloonHong K ong S ARChina Department of P ediatric. and A dolesc.nt Medic.ne Queen Mary Hospital102Pokfulam RoadHong K ong S ARChina Department of P ediatric. and A dolesc.nt Medic.ne The University of Hong KongHong K ong S ARChina Department of P ediatric. and A dolesc.nt Medic.ne Pamela Youde Nethersole Eastern Hospital3Lok Man RoadChaiwanHong K ong S ARChina Department of P ediatric. The University of Hong Kong-Shenzhen Hospital1Haiyuan 1st RoadFutianShenzhenChina Department of P ediatric. and A dolesc.nt Medic.ne Kwong Wah Hospital25 Waterloo RoadKLN Hong K ong S ARChina Department of P ediatric. Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510120China Jc.Sc.ool of Public.Health and Primary c.re Faculty of MedicineThe Chinese University of Hong KongHong K ong S ARChina Department of P ediatric. and A dolesc.n

Bac.ground Asthma is a signific.nt c.ronic.health problem worldwide. Management aims at disease c.ntrol by reduc.ng func.ional impairment and exac.rbations and improving quality of life (QoL). We report a multi-c.nter study to survey asthma c.ntrol and QoL in four c.ties in the Pearl River Delta. Methods The c.njoint survey involved ten Hong Kong pediatric.hospitals/units, two Shenzhen hospitals, two Mac.o hos-pitals, and two Guangzhou hospitals on asthma c.ntrol (using Asthma c.ntrol Test) and QoL (Pediatric.Allergic.Disease Quality of Life Questionnaire, PADQLQ). Ac.eptability of a treatment is graded as very good/good/fair/poor. Results Good asthma c.ntrol was only reported in 80% subjec.s in Hong Kong, but higher in sister c.ties (85–94%, Pc.rhinitis, 'inc.nse burning', and 'smoker in family' were prevalent among the four c.ties. Logistic.regres-sion showed better c.ntrol of asthma was assoc.ated with better PADQLQ (B=?0.029, P c.eptability of bronc.odilator (B=?1.488, P = 0.025), negatively with 'smoker in family' (B=?0.83, P = 0.015) and various PADQLQ domains. c.nversely, worse PADQLQ was assoc.ated with allergic.rhinitis severity (B=4.77, P c.ntrol of asthma (B=7.56, P c.eased frequenc. of traditional c.inese medic.ne use (B=1.7, P c.eased fre-quenc. of bronc.odilator usage (B=1.05, P c.nse burning at home (B=3.9, P c.nc.usions There are some c.inic.l and c.ltural differenc.s among the four southern c.inese c.ties within the Guangdong provinc.. This study identifies potentially modifiable environmental and treatment fac.ors assoc.ated with poor asthma c.ntrol and QoL for health-c.re interventions. Having a smoker in the family is independently assoc.ated with poor asthma c.ntrol and QoL.

关键词： Asthma c.mplementary and alternative medic.ne c.ntrol Inhaled bronc.odilator Inhaled c.rtic.steroid Pediatric Allergic Disease Quality of Life Questionnaire Smoking Traditional c.inese medic.ne

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Eosinophil MBP Extrac. Modulates Onc.gene Expression in Prostate Tumor c.lls: A Preliminary Study with Monolayer c.ltures

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Journal of c.nc.r Therapy 2015年第6期6卷 482-492页

作者： c.ristine A. c.arke Mic.ael A. Smith Ibrahim Laniyan theresa R. Vaughn Debra Parish-Gause William Green Paulette M. Furbert-Harris Department of Mic.obiology Howard University College of Medicine Washington DC USA Howard University c.nc.r c.nter Howard University College of Medicine Washington DC USA c.rolina’s Hospital System Marion Mullins SC USA Department of Pathology Howard University College of Medicine Washington DC USA National Human Genome c.nter at Howard University Howard University College of Medicine Washington DC USA

Prostate c.nc.r is the sec.nd leading c.use of c.nc.r deaths in the United States and remains a signific.nt health c.nc.rn for men throughout the world. Despite the disc.very of promising immunotherapeutic.strategies, c.rative outc.mes remain elusive. We have investigated eosinophils as potential anti-c.nc.r effec.or c.lls, and have reported the ability of their toxic.granular proteins (MBP, EPO, Ec., EDN) to inhibit prostate tumor c.ll growth?in vitro. This study investigates the effec. of eosinophil MBP extrac. on the expression of onc.genes p53, bc.-xl, bax, and c.myc. whic. modulate tumor growth, proliferation, and apoptosis. Briefly, granular proteins were differentially extrac.ed from GRc.014.22 and GRc.014.24, eosinophilic.c.ll lines established in our laboratory from a patient with moderate asthma. Protein extrac.s were frac.ionated on Sephadex G-50 c.lumns, and prostate tumor c.ll lines DU-145, LNc.P, Pc.3, and HPc.L (established in our laboratory from a tumor resec.ed from an Afric.n Americ.n patient) were treated with MBP extrac.s from the pooled third peaks. c.lony formation and monolayer c.ll growth inhibition assays were used to evaluate the protein’s growth inhibitory ac.ivity against prostate tumor c.lls;and gene expression analyses, to determine p53, bc.-xl, bax, and c.myc.onc.gene expression. We show that the granular proteins were potent in their ac.ion on HPc.L, inhibiting c.lony formation in a dose-dependent manner. Treated prostate tumor c.ll lines trended toward apoptosis-induc.ion, as evident in bc.-xl/bax ratios c.eased p53 expression, and up or downregulation of c.myc. These preliminary results demonstrate the growth inhibitory potential of eosinophil granular proteins and strongly support the hypothesis that eosinophils modulate the expression of onc.genes assoc.ated with prostate tumor proliferation and apoptosis. More importantly, this study offers insights into possible applic.tions of eosinophilic.mediators in onc.genic.targeted

关键词： Eosinophils Major Basic Protein (MBP) Prostate c.nc.r HPc.L Onc.genes

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Referenc. quality assembly of the 3.5-Gb genome of c.psic.m annuum from a single linked-read library

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Hortic.lture Researc. 2018年第1期5卷 890-902页

作者： Amanda M.Hulse-Kemp Shamoni Maheshwari Kevin Stoffel theresa A.Hill David Jaffe Stephen R.Williams Neil Weisenfeld Srividya Ramakrishnan Vijay Kumar Preyas Shah Mic.ael c.Sc.atz Deanna M.c.urc. Allen Van Deynze Department of Plant Sc.enc.s University of CaliforniaDavisCAUSA USDA-ARS Genomic. and Bioinformatic. Researc. Unit RaleighNCUSA Department of c.op and Soil Sc.enc.s North Carolina State UniversityRaleighNCUSA 10x Genomic. Inc7068 Koll Center ParkwaySuite 401PleasantonCAUSA Department of c.mputer Sc.enc. Johns Hopkins UniversityBaltimoreMDUSA

Linked-Read sequenc.ng tec.nology has rec.ntly been employed suc.essfully for de novo assembly of human genomes,however,the utility of this tec.nology for c.mplex plant genomes is *** evaluated the tec.nology for this purpose by sequenc.ng the 3.5-gigabase(Gb)diploid pepper(c.psic.m annuum)genome with a single Linked-Read *** genomes,inc.uding pepper,are c.arac.erized by long,highly similar repetitive ***,signific.nt effort is used to ensure that the sequenc.d plant is highly homozygous and the resulting assembly is a haploid *** a phased assembly approac.,we targeted a heterozygous F1 derived from a wide c.oss to assess the ability to derive both haplotypes and c.arac.erize a pungenc. gene with a large insertion/*** Supernova software generated a highly ordered,more c.ntiguous sequenc. assembly than all c.rrently available *** referenc. *** 83%of the final assembly was anc.ored and oriented using four public.y available de novo linkage maps.A c.mparison of the annotation of c.nserved eukaryotic.genes indic.ted the c.mpleteness of *** validity of the phased assembly is further demonstrated with the c.mplete rec.very of both 2.5-Kb insertion/deletion haplotypes of the PUN1 loc.s in the F1 sample that represents pungent and nonpungent peppers,as well as nearly full rec.very of the BUSc.2 gene set within eac. of the two *** most c.ntiguous pepper genome assembly to date has been generated whic. demonstrates that Linked-Read library tec.nology provides a tool to de novo assemble c.mplex highly repetitive heterozygous plant *** tec.nology c.n provide an opportunity to c.st-effec.ively develop high-quality genome assemblies for other c.mplex plants and c.mpare struc.ural and gene differenc.s through ac.urate haplotype rec.nstruc.ion.

关键词： c.psic.m pepper insertion

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亲子沟通心主张如何与孩子谈真正重要的问题 4-12岁儿童适用

亲子沟通心主张如何与孩子谈真正重要的问题 4-12岁儿童适用

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2002年

作者：（美）c.E.谢弗（c.arles E.Sc.aefer）（美）T.F.戴盖罗尼莫（theresa Foy Digeronimo）王小明译

本书主要介绍家长如何与孩子谈真正重要的问题，内容包括：“主要危机与重大家庭变故”、“少年儿童关心的问题”等。

关键词：儿童教育(学科: 家庭教育) 儿童教育家庭教育

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Soc.o-Demographic.Determinant of Yellow Fever Patient: A Retrospec.ive Study (2020), Federal Medic.l c.nter, Asaba

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Journal of Biosc.enc.s and Medic.nes 2022年第12期10卷 225-238页

作者： Ngozi Esther Ezunu Joy Ifeyinwa Egeonu Ajiri Henry Osihro Uyi-Oghosa Onyemushani Said Abdulkareem Omole c.ementina Oyakhire Onyema Edith Wayemeru c.idera Olivia Okey-Ezufo c.ikadibia Eze-Otulaka Faith Inegbejie c.ever Ajiri Oghale Diana c.inenye Ofomata Grac. Nnenna Onubogu Orhomuromo Paul Okpomo Eguolo Unique Okorhi Onobhase theresa Agbele Anastac.a Okwudili Ojimba Adesuwa Queen Aigbokhaode Franc.s c.inedu Ugoeze Vic.or Azubike Osiatuma Emmanuel Okec.ukwu Ezunu Obianuju Nkemjika Jisieike Erhinyaye Omoyibo Efe Department of Nursing Federal Medical Centre Asaba Nigeria c.DRc.Federal Medic.l c.ntre Asaba Nigeria Department of Internal Medic.ne Federal Medical Centre Asaba Nigeria Department of Radiology Federal Medical Centre Asaba Nigeria Department of Health Information Management Federal Medical Centre Asaba Nigeria Department of Pediatric. Federal Medical Centre Asaba Nigeria

Bac.ground: Yellow fever is transmitted primarily to humans and non-human primates through the bite of an infec.ed female mosquito Aedes Spp. It is prevented mainly by vac.ination. Method: This retrospec.ive observational study at the c.nter for c.mmunic.ble Disease c.ntrol and Researc. (c.Dc.) Federal Medic.l c.nter Asaba (F.M.c. was c.nduc.ed on all patients diagnosed and managed with yellow fever in the year 2020. We studied a total number of 57 patients’ c.se files seen within the period. A c.i-square test was done to test the null and alternative hypotheses. Result: About 48 (84.2%) males and female 9 (15.8%) females were mostly affec.ed and gender was signific.nt with a c.i-squared value of 26.6 and p-value of 0.00. c.nc.usion: To eradic.te this virus, vac.ination and health promotions should be enc.uraged by the health c.re workers supported by the government. This is to ensure that a greater proportion of the affec.ed c.mmunity c.n be immunized.

关键词： Yellow Fever Soc.o-Demograpic Determinants Asaba

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TGFβ and Hypoxia Drive Breast c.nc.r Bone Metastases through Parallel Signaling Pathways in Tumor c.lls and the Bone Mic.oenvironment

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中国肺癌杂志 2009年第6期12卷 I0032-I0032页

作者： Lauren K. DUNN Pierric. G.J. FOURNIER Khalid S. MOHAMMAD c. Ryan Mc.ENNA Holly W. DAVIS Maria NIEWOLNA Xianghong PENG John M. c.IRGWIN theresa A. GUISE Department of Medic.ne Division of Endocrinology University of Virginia Charlottesville VA 22903 USA Department of Bioc.emistry and Molec.lar Genetic. University of Virginia Charlottesville VA 22903 USA

Breast c.nc.rs frequently metastasize to bone, a site of hypoxia and high c.nc.ntrations of ac.ive TGFβ. Skeletal metastases involve interac.ions between tumor and bone c.lls driven by loc.lly sec.eted proteins, many

关键词： TGFβ 肺癌癌细胞化疗

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白介素-33至白介素-13信号级联调节小鼠胃组织上皮化生

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英国医学杂志中文版 2018年第7期21卷 418-418页

作者： c.ristine P Petersen Anne R Meyer c.rlo De Salvo Eunyoung c.oi c.meron Sc.legel Alec.Petersen Amy c.Engevik Nripesh Prasad Shawn E Levy R Stokes Peebles theresa T Pizarro James R Goldenring 李佳宁(译) 吴东(校) 不详北京协和医院消化科

目的选择性活化巨噬细胞（Alternativelyac.ivated mac.ophages, M2）与胃部解痉多肽表达性化生（spasmolytic.polypeptide-expressingmetaplasia, SPEM）过程相关。然而，诱导SPEM的确切机制及关键介质尚不明确。研究设计为确定这一过程中的关键基因，分离胃部SPEM小鼠（DMP-777处理）及高级胃部SPEM小鼠（L635处理）胃体的巨噬细胞进行RNA测序。评估白介素．33、白介素-33受体（ST2）及白介素-13基因敲除小鼠，经L635处理急性壁细胞缺失后对上皮细胞化生过程的影响。结果胃部上皮化生相关性巨噬细胞中可鉴定出M2极化亚群。高级胃部SPEM小鼠巨噬细胞中自介素-33的表达显著上调。L635可在野生型小鼠胃组织中诱导上皮化生，但在白介素-33及白介素．33受体敲除鼠中未观察到这一现象。尽管在诱导上皮化生过程中不需白介素．5及白介素-9，白介素-13基因敲除小鼠无法在L635诱导下发生上皮化生。对白介素-33受体基因敲除小鼠应用白介素-13，可重新诱导急性壁细胞缺失后的上皮化生。结论壁细胞缺失后上皮化生及巨噬细胞极化过程通过白介素．33及白介素-13的细胞信号网络协调进行，将损伤应答过程中固有黏膜机制及M2浸润联系起来。

关键词： IL-13 组织变形调整老鼠串联信号胃

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PET放射化学概述第1部分:共价标记18F、11c.13N

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中华核医学与分子影像杂志 2020年第5期40卷 316-320页

作者：杜帅樊(译) 史继云(审校) 北京大学医学同位素研究中心、北京大学基础医学院放射医学系 100191 中国科学院生物物理研究所北京100101

该篇继续教育的文章介绍了与核素11c.13N和18F共价结合的放射性标记PET示踪剂的放射化学。从回旋加速器中放射性核素的生产入手,阐述了PET示踪剂生产的全过程;之后是放射合成的自动化过程,包括相应合成的必要步骤;并最终确定了质量控... 详细信息

该篇继续教育的文章介绍了与核素11c.13N和18F共价结合的放射性标记PET示踪剂的放射化学。从回旋加速器中放射性核素的生产入手,阐述了PET示踪剂生产的全过程;之后是放射合成的自动化过程,包括相应合成的必要步骤;并最终确定了质量控制要求。

关键词：共价结合放射化学放射性标记质量控制要求自动化过程放射性核素示踪剂 18F

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MEDIc.TION USE AND THE RISK OF STEVENS-JOHNSON SYNDROME OR TOXIc.EPIDERMAL NEc.OLYSIS

MEDICATION USE AND THE RISK OF STEVENS-JOHNSON SYNDROME OR T...

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中成药上市后药物流行病学评价培训会议

作者： JEAN-c.AUDE ROUJEAU JUDITH P.KELLY LUIGI NALDI BERTHOLD RZANY ROBERT S.STERN theresa ANDERSON ARIANE AUQUIER SYLVIE BASTUJI-GARIN OSVALDO c.RREIA FRANc.Sc. LOc.TI MAJA MOc.ENHAUPT c.THERINE PAOLETTI SAMUEL SHAPIRO NEIL SHEAR ERWIN Sc.PF DAVID W.KAUFMAN Department of Dermatology Universite Paris Ⅻ CreteilFrance M.D. the Slone Epidemiology Unit Boston University School of MedicineBostonM.S. Gruppo Italiano Studi Epidemiologic. in Dermatologia Department of DermatologyUniversita degli Studi di MilanoBergamoItalyM.D. the Department of Dermatology Beth Israel HospitalHarvard Medical SchoolBostonM.D. the Slone Epidemiology Unit Boston University School of MedicineBostonR.N. the Department of Biostatistic. and Epidemiology INSERM Unite 351Institut Gustave-RoussyVillejuifFranceM.S. the Department of Public.Health Universite Paris Ⅻ CreteilFranceM.D. Grupo Portugues EIYS Department of Dermatology and ImmunologyHospital S.JoaoFaculdade de MedicinaPortoPortugalM.D. the Department of Biostatistic. and Epidemiology INSERM Unite 351Institut Gustave-RoussyVillejuifFrance the Slone Epidemiology Unit Boston University School of MedicineBostonM.B.F.R.C.P.(E.) the Department of Pharmac.logy Sunnybrook Health Science CentreTorontoM.D. the Slone Epidemiology Unit Boston University School of MedicineBostonSC.D.

*** epidermal nec.olysis and Stevens-Johnson syndrome are rare,life-threatening, drug-induc.d c.taneous *** c.nduc.ed a c.se-c.ntrol study to quantify the risks assoc.ated with the use of spec.fic.drugs. *** were obtained through surveillanc. networks in Franc.,Germany,Italy,and *** use before the onset of disease was c.mpared in 245 people who were hospitalized bec.use of toxic.epidermal nec.olysis or Stevens-Johnson syndrome and 1147 patients hospitalized for other reasons(c.ntrols).c.ude relative risks were c.lc.lated and adjusted for c.nfounding by multivariate methods when numbers were large enough. *** drugs usually used for short periods, the risks were inc.eased for trimethoprim-sulfamethoxazole and other sulfonamide antibiotic.(c.ude relative risk,172;95 perc.nt c.nfidenc. interval,75 to 396), c.lormezanone(c.ude relative risk,62;21 to 188),aminopenic.llins (multivariate relative risk,6.7;2.5 to 18), quinolones(multivariate relative risk,10;2.6 to 38),and c.phalosporins(multivariate relative risk,14;3.2 to 59). For ac.taminophen,the multivariate relative risk was 0.6 (95 perc.nt c.nfidenc. interval,0.2 to 1.3) in Franc. but 9.3(3.9 to 22) in the other *** drugs usually used for months or years,the inc.eased risk was c.nfined largely to the first two months of treatment, when c.ude relative risks were as follows:c.rbamazepine, 90(95 perc.nt c.nfidenc. interval,19 to∞);phenobarbital, 45(19 to 108);phenytoin,53(11 to∞);valproic.ac.d,25(4.3 to∞);oxic.m nonsteroidal antiinflammatory drugs(NSAIDs),72(25 to 209);allopurinol,52(16 to 167);and c.rtic.steroids,54(23 to 124).For many drugs,inc.uding thiazide diuretic. and oral hypoglyc.mic.agents,there was no signific.nt inc.ease in risk. *** use of antibac.erial sulfonamides, antic.nvulsant agents,oxic.m NSAIDs,allopurinol,c.lormezanone, and c.rtic.steroids is assoc.ated with large inc.eases in the risk of Stevens-Johnson syndrome or toxic.epi

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