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Seroprevalence of HBV and HCV among People Living with HIV in Burkina Faso and Diagnostic Performance of HIV/HCV/HBsAg Combined Rapid Test in Comparison with Architect Assays

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Open Journal of Medical Microbiology 2024年第1期14卷 1-10页

作者： Serge Theophile Soubeiga Albert theophane Yonli Alain Megabtche Nantchouang Jacques Simpore Department of Biomedical and Public Health Research Institute for Health Sciences (IRSS) Ouagadougou Burkina Faso Pietro Annigoni Biomolecular Research Centre (CERBA) Ouagadougou Burkina Faso Laboratory of Molecular Biology and Genetics (LABIOGENE) University Joseph Ki-Zerbo Ouagadougou Burkina Faso BIOMEX Laboratory Heidelberg Germany

Background: The diagnosis of human immunodeficiency virus (HIV), hepatitis B virus (HBV), and hepatitis C virus (HCV) remains a constraint for some populations in sub-Saharan Africa. This study aimed to determine the prevalence of HBV and HCV in people living with HIV and to evaluate the performance of a combined rapid test for the simultaneous detection of HIV, HBV, and HCV. Methods: This is a cross-sectional study that took place from February 2017 to November 2018 and included 139 HIV-infected individuals followed up at different medical centers in Ouagadougou, Burkina Faso. HBV and HCV serology tests were performed on-site using finger prick whole blood with HIV/HCV/HBsAg combined rapid test and then serum with two reference tests “Architect HBsAg Qualitative” and “Architect HIV Ag/Ab Combo”. Results: The mean age of the participants was 57 ± 8 years. Of the 139 participants, 10% (14/139) were HIV-1 positive, 71.9% (100/139) were HIV-2 positive, and 18.0% (25/139) were HIV-1/HIV-2 coinfected. The sensitivity and specificity of the HIV/HCV/HBsAg combined rapid test were 33.33% vs 99.11% and 20% vs 99.25% compared to Architect HBsAg Qualitative and Architect HIV Ag/Ab Combo, respectively. The Kappa and Youden Index values were 0.4262 and 0.3244 and 0.2707 and 0.1925, respectively, compared to each of the two reference tests. Conclusion: The results show that the HIV/HCV/HBsAg combined rapid test has poor diagnostic efficiency and should not be recommended for the diagnosis of these viruses.

关键词： HIV HBV HCV Prevalence Diagnosis Burkina Faso

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Vibration Analysis of Vehicle Floor Panel Using Hybrid Method of FEM and SEA

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Journal of Mechanics Engineering and Automation 2014年第4期4卷 305-310页

作者： Kazuhito Misaji Yusuke Suzuki Ayumi Takahashi Fumihiko Ide theophane courtois Department of lndustrial Technology Nihon University Chiba 275-8575 Japan Honda R&D Americas Inc. Raymond 43067 America autoneum Winterthur 43 8406 Switzerland

In order to improve the mass efficiency of an automotive soundproof package, it is important to predict the middle to high frequency range of noise and vibration during vehicle operation. A hybrid method of experimental and analytical SEA (statistical energy analysis) has been applied for the prediction of air-borne noise. However, for predicting structure-borne noise, there are no definitive simulation methods that can address the soundproof specifications in an actual vehicle. Thus, in this paper, a FEM (finite element method) · SEA hybrid method is used. The FEM · SEA hybrid method predicts structure-borne noise in the middle to high frequency range. First, we explain the basic concept of the FEM · SEA hybrid method; Second, we describe our experiment to verify the analytical results of the FEM · SEA hybrid method; Third, we provide the details of the FEM model versus the FEM · SEA hybrid model; Finally, we verify the validity and availability of the FEM · SEA hybrid method through comparisons of the FEM analysis results, FEM · SEA analysis results and measured results.

关键词：混合方法振动分析 SEA 有限元法车辆结构噪声面板地板

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Molecular Diagnosis of Sexual Differentiation Disorders in Burkina Faso

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Journal of Biosciences and Medicines 2024年第3期12卷 1-15页

作者： Bazie Bapio Valérie Elvira Jean Télesphore Sawadogo Soumaïla Zohoncon Théodora Mahoukèdè Tall Ahmadou Bado Prosper Soubeiga Serge Théophile Ouattara Moussa Sorgho Abel Kiendrebeogo Touwendpoulimdé Isabelle Yonli Albert theophane Djigma Florencia Millogo/Traore Timongo Françoise Danielle Simpore Jacques Centre National de la Recherche Scientifique et Technologique (CNRST) Institut de Recherche en Sciences de la Santé (IRSS) Ouagadougou Burkina Faso Université Joseph KI-ZERBO Unité de Formation en Sciences de la Vie et de la Terre Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigonie (CERBA) Ouagadougou Burkina Faso Centre Hospitalier Universitaire Régional de Ouahigouya Ouagadougou Burkina Faso Centre Hospitalier Universitaire Yalgado Ouedraogo Université Joseph Ki-Zerbo Ouagadougou Burkina Faso

Sex development anomalies represent a group of congenital pathologies in which chromosomal, gonadal and anatomical sex differentiation is atypical. The aim of our study was to use molecular biology techniques to elucidate sex in cases of anomalies of sexual differentiation in Burkina Faso. This cross-sectional study took place from March 2023 to June 2023. Oral and blood samples were collected respectively using sterile swabs and stored on Swab Kits and NUCLEIcardTM (https://***/product-ranges/nucleic-card/) were used to determine gonosome profiles. Extraction was carried out using the DNA Swap solution Kit and the DNA IQ System Kit, and a PowerPlex? 21 kit (Promega) for amplification. The Applied Biosystems 9700 thermal cycler was used for PCR followed by 36 cm capillary electrophoresis in the Applied Biosystems 3130 prism sequencer. Sequence files were analyzed using GeneMapper IDX v. 3.2 software. Seven (07) patients were registered during the study period. There were 4 cases of XX DSD or 46, XX DSD and 3 cases of XY DSD or 46, XY DSD. The median age of our patients was 16 years. Civilian sex was male in 4 cases and female in 3. The most frequent reason for consultation was micropenis in 3 cases, followed by primary amenorrhea and sex ambiguity. There were 03 cases of discrepancy between genetic sex and civil sex. The accessibility of molecular diagnosis is little known to clinicians. XX DSDs or 46, XX DSDs were the most frequent (4/7) in our study. The problem facing this situation is early diagnosis to help prevent complications in Burkina Faso.

关键词： Gonosome DSD Abnormal Sexual Development Burkina Faso

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Genetic diversity of hepatitis viruses in West-African countries from 1996 to 2018

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World Journal of Hepatology 2018年第11期10卷 807-821页

作者： Maléki Assih Abdoul Karim Ouattara Birama Diarra Albert theophane Yonli Tegwindé Rebeca Compaore Dorcas Obiri-Yeboah Florencia Wendkuuni Djigma Simplice Karou Jacques Simpore Bioche-mistry-Microbiology CERBA/LABIOGENEOuagadougou 02006Burkina Faso Laboratoryof Molecular Biology and Molecular Genetics(LABIOGENE)UFR/SVT University Ouaga I Prof Joseph KI-ZERBOOuaga-dougou 00226Burkina Faso Department of Microbiology and Immunology School of Medical SciencesUniversity of Cape CoastCape Coast 00233Ghana Ecole Supérieure des Techniques Biologiques et Alimentaires(ESTBA-UL) Universite de LomeLome 00229Togo

The severity of hepatic pathology and the response to treatment depend on the hepatitis virus genotype in the infected host. The objective of this review was to determine the distribution of hepatitis virus genotypes in West African countries. A systematic review of the literature in PubMed, Google Scholar and Science Direct was performed to identify 52 relevant articles reporting hepatitis A, B, C, D, E and G viruses genotypes.Hepatitis B virus(HBV) genotype E with a prevalence of 90.6%(95%CI: 0.891-0.920) found in this review, is characterized by low genetic diversity. Hepatitis C virus(HCV) genotypes 1 and 2 represented 96.4% of HCV infections in West African countries, while hepatitis delta virus, hepatitis A virus, hepatitis G virus genotypes 1 and HEV genotype 3 were reported in some studies in Ghana and Nigeria. HBV genotype E is characterized by high prevalence, low genetic diversity and wide geographical distribution. Further studies on the clinical implications of HBV genotype E and HCV genotypes 1 and 2 are needed for the development of an effective treatment against this viral hepatitis in West African countries. Surveillance of the distribution of different genotypes is also needed to reduce recombination rates and prevent the emergence of more virulent viral strains.

关键词：肝病乙肝病毒治疗方法

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Risk Factors Associated with Mother-to-Child Transmission of HIV-1, Spontaneous Abortion and Infant Mortality in HIV-1 Infected Women in Burkina Faso: A Prospective Study

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Journal of Biosciences and Medicines 2018年第10期6卷 12-21页

作者： Serge Theophile Soubeiga Albert theophane Yonli Tegwindé Rebeca Compaore Abdoul Karim Ouattara Birama Diarra Dorcas Obiri-Yeboah Zoenabo Douamba Lassina Traore Wendkuuni Florencia Djigma Jacques Simpore Pietro Annigoni Biomolecular Research Centre (CERBA) Ouagadougou Burkina Faso Laboratory of Molecular Biology and Genetics (LABIOGENE) University Ouaga I Prof. Joseph KI-ZERBO Ouagadougou Burkina Faso Department of Microbiology and Immunology School of Medical Sciences University of Cape Coast Cape Coast Ghana

Background: Despite efforts to fight, HIV/AIDS mother-to-child transmission of HIV-1 (MTCT), as well as abortion and infant mortality, remains a problem in sub-Saharan Africa. Indeed, a low level of CD4 and a high viral load can be associated with these situations. The aim of this study was to determine the risk factors associated with the occurrence of MTCT, spontaneous abortion and infant mortality in HIV-1 infected women in Ouagadougou, Burkina Faso. This was a prospective study conducted from May 2014 to September 2017 and involved 423 HIV-1 infected women followed at Saint Camille Hospital in Ouagadougou, Burkina Faso. Sociodemographic data were collected through a questionnaire. The CD4 count and HIV-1 viral load were determined using respectively BD FACSCount and Abbott m2000rt instruments. Bivariate analysis and multinomial logistic regression were performed for associations with a significance threshold for p Results: The average age of women was 38.75 ± 7.98 years. Rates of MTCT, abortion and infant mortality were 16.31%, 30.49% and 34.75%, respectively. The number of pregnancies was associated with the number of infant deaths (p = 0.002). A correlation between the number of pregnancies and infant mortality was observed (p = 0.002) with a relatively high rate (28.6%) among women who had three pregnancies. In addition, marital status was associated with HIV-1 infection in infants (p = 0.042) and spontaneous abortion (p = 0.033). HIV-1 infected women with low CD4 counts (less than 350 cells/μL) and those with viral load more than 1000 copies/mL were about twice as likely to have an spontaneous abortion [OR (IC 95%): 2.50 (1.085 - 5.760);p = 0.03] and [OR (95% CI): 2.16 (1.043 - 4.505);p = 0.04]. Conclusions: The results of this study show the need to improve the treatment of HIV-1 infected women in order to restore CD4 levels and make viral load of HIV-1 undetectable.

关键词： HIV-1 MTCT Abortion Infants Burkina Faso

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