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Spatial distribution of insecticide resistant Check update populations of Aedes aegypti and Ae. albopictus and first detection of V410L mutation in Ae. aegypti from Cameroon

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Infectious Diseases of Poverty 2022年第4期11卷 96-97页

作者： Matthew Montgomery James F.Harwood Aurelie P.Yougang theodel a.wilson.bahun.armel n.tedjou Christophe Rostand Keumeni Auston Marm Kilpatrick Charles S.Wondj Basile Kamgang Centre for Research in Infectious Diseases P.O.Box 13591YaoundeCameroon 不详

Background:Dengue(DEnV),chikungunya(CHIKV)and Zika virus(ZIKV),are mosquito-borne viruses of medical importance in most tropical and subtropical *** control,primarily through insecticides,remains the primary method to prevent their ***,we evaluated insecticide resistance profles and identifed important underlying resistance mechanisms in populations of Aedes aegypti and *** from six different regions in Cameroon to pesticides commonly used during military and civilian public health vector control ***:Aedes mosquitoes were sampled as larvae or pupae between August 2020 and July 2021 in six locations across Cameroon and reared until the next generation,*** and *** adults from G1 were tested following World Health Organization(WHO)recommendations and *** GO adults screened with real time melting curve qPCR analyses to genotype the F1534C,V1016l and V410L Aedes kdr *** butoxide(PBO)assays and real time qPCR were carried out from some cytochrome p450 genes known to be involved in metabolic *** analyses were performed using Chi-square test and generalized linear ***:Loss of susceptibility was observed to all insecticides *** rates from tests with 0.25%permethrin varied from 24.27%to 85.89%in *** and from 17.35%to 68.08%in *** rates for 0.03%deltamethrin were between 23.30%and 88.20%in *** and between 69.47%and 84.11%in *** found a moderate level of resistance against bendiocarb,with mortality rates ranging from 69.31%to 90.26%in *** and from 86.75%to 98.95%in *** PBO pre-exposure,we found partial or fully restored suscepti bility to pyrethroids and *** genes Cyp9M6F88/87 and Cyp9J10 were overexpressed in *** populations from Douala sites resistant to permethrin and ***6P12 was highly expressed in alphacypermethrin and permethrin resistant A

关键词： Arbovirus Aedes aegypti Aedes albopictus Insecticide resistance Knock down resistance Cytochrome P450 monooxygenases Cameroon

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First detection of F1534C knockdown resistance mutation in Aedes aegypti(Diptera:Culicidae)from Cameroon

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Infectious Diseases of Poverty 2020年第6期9卷 51-62页

作者： Aurelie P.Yougang Basile Kamgang theodel a.wilson.bahun.armel n.tedjou Daniel nguiffo-nguete Flobert njiokou Charles S.Wondji Centre for Research in Infectious Diseases P.O.Box 13591YaoundéCameroon Parasitology and Ecology Laboratory Department of Animal Biology and PhysiologyFaculty of ScienceUniversity of Yaoundé1P.O.Box 812YaoundéCameroon Laboratory of Vertebrate and Invertebrate Bioecology Faculty of Science and TechnologyMarien-Ngouabi UniversityBrazzavilleCongo Laboratory of Biology and Applied Ecology Department of Animal BiologyFaculty of ScienceUniversity of DschangP.O.Box 96DschangCameroon Liverpool School of Tropical Medicine Pembroke placeLiverpoolL35QAUK

Background: Aedes borne viral diseases,notably dengue,are increasingly reported in Cameroon with Aedes aegypti being a major *** on insecticide resistance of this vector and underlying mechanisms needed for outbreak preparedness remain scarce in ***,we present the nationwide distribution of insecticide resistance in *** and investigate the potential resistance mechanisms ***:: Immature stages of *** were collected between March and July 2017 in 13 locations across Cameroon and reared until G1/G2/G3 ***,adult bioassays,and piperonyl butoxide(PBO)synergist assays were carried out according to World Health Organization guidelines.F1534C mutation was genotyped using allele specific polymerase chain reaction in field collected adults(Go)and the polymorphism of the sodium channel gene was ***χ2 test was used to compare the mortality rate between bioassays with insecticides only and bioassays after preexposure to PBO ***: Larval bioassay revealed that all the three populations tested with temephos were *** bioassays showed a good level of susceptibility toward both pyrethroids tested,0.25%permethrin and 0.05%deltamethrin,with six out of 10 populations ***,two populations(Douala and Edéa)were resistant(deltamethrin[73.2–92.5%mortality],permethrin[2.6–76.3%mortality]).The resistance to 4%dichlorodiphenyltrichloroethane was observed in four out of 10 populations tested(16.8–87.1%mortality).Resistance was also reported to carbamates including 0.1%propoxur(60.8–87.1%mortality)and to 0.1%bendiocarb(82.9%mortality).All populations tested were fully susceptible to 1%fenitrothion.A partial recovery of susceptibility was observed in the pyrethroid resistant population of Douala after pre-exposed to PBO suggesting the implication of cytochrome P450 monoxygenases permethrin *** and sequencing detected the F1534C kdr mutation in the two pyrethroid resistant

关键词： Aedes aegypti Insecticide resistance Kdr mutation Cameroon Arbovirus

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Contrasting resistance patterns to type Ⅰ and Ⅱ pyrethroids in two major arbovirus vectors Aedes aegypti and Aedes albopictus in the Republic of the Congo, Central Africa

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Infectious Diseases of Poverty 2020年第2期9卷 95-95页

作者： Basile Kamgang theodel A.wilson-bahun Aurelie P.Yougang Arsene Lenga Charles S.Wondji Centre for Research in Infeaious Diseases Department of Medical EntomologyPO Box 15391YaoundeCameroon 不详

Background:In the Republic of Congo,with two massive outbreaks of chikungunya observed this decade,little is known about the insecticide resistance profile of the two major arbovirus vectors Aedes aegypti and Aedes ***,we established the resistance profile of both species to insecticides and explored the resistance mechanisms to help Congo to better prepare for future ***:Immature stages o f *** and *** were sampled in May 2017 in eight cities of the Republic of the Congo and reared to adult *** and adult bioassays,and synergist(piperonyl butoxide[PBO])assays were carried out according to WHO guidelines.F1534C mutation was genotyped in field collected adults in both species and the polymorphism of the sodium channel gene assessed in ***:All tested populations were susceptible to temephos after larval bioassays.A high resistance level was observed to 4%DDT in both species countrywide(21.9-88.3% mortality).All but one population(*** from ngo)exhibited resistance to type I pyrethroid,permethrin,but showed a full susceptibility to type II pyrethroid(deltamethrin)in almost all *** was also reported to 1% propoxur in *** likewise in two *** populations(Owando and Ouesso),and the remaining were fully *** populations of both species were fully susceptible to 1%fenitrothion.A full recovery of susceptibility was observed in *** and *** when pre-exposed to PBO and then to propoxur and permethrin *** F1534C kdr mutation was not detected in either *** high genetic variability of the portion of sodium channel spanning the F1534C in *** further supported that knockdown resistance probably play no role in the permethrin ***:Our study showed that both Aedes species were susceptible to organophosphates(temephos and fenitrothion),while for other insecticide classes tested the profile of resistance vary a

关键词： Aedes oegypti Aedes albopictus Insecticide resistance Resistance mechanism Republic of the Congo

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