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Infrared multiphoton dissociation of aceticanhydride:experimental study

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Chinese Journal of Chemistry 1990年第4期8卷 306-312页

作者： XIANG, Tian-Xiang Chemical Physics laboratory, Department of Chemistry, university of nanjing,{3. Chemical Physics laboratory Department of Chemistry University of Nanjing Nanjing 210008

The infrared multi-photon dissociation (IRMPD) of acetic anhydride to generate state- selected CH_2 has been investigated by using time and wavelength resolved fluorescence techniques. It has been observed that the generation of the nascent ground state CH_2 (a^1A_1) is delayed by about 0.6 μs relative to the onset time of the excited CH_2(b^1B_1). The relative population of the ground and excited state CH_2 and the onset time for the appearance of the ground state CH_2 have been found to depend strongly on CO_2 laser output fluences. These results appear to have a strong bearing on the structure of the potential energy surfaces of the intermediate CH_2CO. A possible photolysis me- chanism is proposed to explain these experimental results.

关键词： CO nm Infrared multiphoton dissociation of aceticanhydride Figure

Study on lithogeochemistry of Middle Jurassic basalts from southern China represented by the Fankeng basalts from Yongding of Fujian Province

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Science China Earth Sciences 2006年第10期49卷 1020-1031页

作者： ZHOU Jincheng1,2, JIANG Shaoyong1,2, WANG Xiaolei1,2, YANG Jinghong 1,2 & ZHANG Mengqun 3.1. state Key laboratory of Mineral Deposit Research, nanjing university, {3. 210093. China 2. Department of Earth Sciences, nanjing university, {3. 210093. China 3. Modern Analysis Center, nanjing university, {3. 210093. China 1. state Key laboratory of Mineral Deposit Research Nanjing University Nanjing 210093 China 2. Department of Earth Sciences Nanjing University Nanjing 210093 China 3. Modern Analysis Center Nanjing University Nanjing 210093 China

There exists an E-W trending Middle Jurassic volcanic zone in southern China. The Fankeng basalts in the Yongding basin of Fujian Province are considered to be a typical example. The Fankeng basalts have TiO2 contents in the range of 1.92%-3.21%. They are classified as high-Ti basalts. They also have higher total Fe (averaging FeO* = 11.09%). The Middle Jurassic Fankeng basalts from southwestern Fujian have obvious distinctive lithogeochemical features from early Cre- taceous basalts from southeastern coast of China. They have higher HFSE, such as Th, Nb, Ta, Zr and Ti. Their element ratios related with HFSE, such as Zr/Ba, La/Nb, La/Ta ,Zr/Y, Ti/Y, Ba/Nb, K/Ti and Rb/Zr are similar to those of OIB. The most samples have ε Nd(T) of-0.70-0.24, which are near chondrite. Some samples have higher ε Nd(T) of ***, these basaltic magmas might be derived from depleted asthenospheric mantle. The lithogeochemical characteristics of the Fankeng basalts may be caused by interaction between asthenosphere and lithosphere at the time. The (Early-) Middle Jurassic basalts and gabbros from southeastern Hunan, southern Jiangxi and northern Guangdong provinces show similar geochemical features to those of the Fankeng basalts from the Yongding of Fujian. Occurrence of these OIB-type basalts in the area may be regarded as the petrological mark of upwelling of asthenosphere at the time. Upwelling of asthenosphere has led to tectonic extension and the formation of rifted basin in the area.

关键词： interaction between asthenosphere and lithosphere, lithogeochemistry, basalts, Middle Jurassic, southern China.

Polymer-assisted nanoparticulate contrast-enhancing materials

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Science China Chemistry 2010年第3期53卷 479-486页

作者： DING Yin1,3.HU Yong2,WU Wei1 & JIANG XiQun1 1laboratory of Mesoscopic Chemistry and Department of Polymer Science and Engineering,College of Chemistry & Chemical Engineering,nanjing university,nanjing 210093.China 2national laboratory of Solid state Microstructure,Department of Material Science and Engineering,nanjing university,nanjing 210093.China 3.ey laboratory of Analytical Chemistry for Life Science,Ministry of Education,School of Chemistry and Chemical Engineering,nanjing university,nanjing 210093.China 1. laboratory of Mesoscopic Chemistry and Department of Polymer Science and Engineering College of Chemistry & Chemical Engineering Nanjing University Nanjing 210093 China3. Key Laboratory of Analytical Chemistry for Life Science Ministry of Education School of Chemistry and Chemical Engineering Nanjing University Nanjing 210093 China2. National Laboratory of Solid State Microstructure Department of Material Science and Engineering Nanjing University Nanjing 210093 China

The application of nanotechnology in medicine research has significant potential in modern biomedical research,disease diagnosis and *** fluorophore-based detection techniques have been widely used as imaging and signal transduction tools for the detection of trace levels of *** photosensitivity of the fluorophores,however,limits their application in such complex environments as living bio-systems where degradation or photobleaching *** nanoparticles have unique and stable optical,electrical,magnetic and catalytic properties because of their various shapes,sizes and *** potential of the nanoparticles as novel imaging agents has been studied as a possible solution to this *** modification of inorganic nanoparticles could enhance their biostability in physiological conditions and ability of targeting bioactive molecules.

关键词： contrast agent cell image gold nanoparticles iron oxide nanoparticles carbon nanotubes polymeric-inorganic hybrid nanoparticles

DISTRIBUTION OF VORTEX BARRIERS AT DIFFERENT TEMPERATURES FOR TL 2212 FILM

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南京大学学报(自然科学) 1999年第4期35卷 500-501页

作者： Lin Fenyong Ding Shiying (national laboratory of Solid state {3. and Department of Physics, nanjing university,210093.nanjing,PRC) 南京大学物理系固体微结构物理国家重点实验室江苏

Sensitive biosensing strategy based on functional nanomaterials

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Science China Chemistry 2011年第8期54卷 1202-1217页

作者： JU HuangXian state Key laboratory of Analytical Chemistry for Life Science Department of Chemistry, nanjing university, {3. 210093. China 1. state Key laboratory of Analytical Chemistry for Life Science Department of Chemistry Nanjing University Nanjing 210093 China

The first decade of the 21st century has been labeled as "the sensing decade". The functional nanomaterials offer excellent platforms for fabrication of sensitive biosensing devices, including optical and electronic biosensors. A lot of works have fo- cused on the biofunctionalization of different nanomaterials, such as metal nanoparticles, semiconductor nanoparticles and carbon nanostructures, by physical adsorption, electrostatic binding, specific recognition or covalent coupling. These biofunc- tionalized nanomaterials can be used as catalysts, electronic conductors, optical emitters, carriers or tracers to obtain the ampli- fied detection signal and the stabilized recognition probes or biosensing interface. The designed signal amplification strategies have greatly promoted the development of stable, specific, selective and sensitive biosensors in different fields. This review in- troduces some novel principles and detection strategies in the area of biosensing, based on functional nanomaterials. The gen- eral methods for biofunctionalization of nanomaterials with biomolecules and their biosensing application in immunoassay of protein, DNA detection, carbohydrate analysis and cytosensing are also described.

关键词： nanobiosensing biosensors nanomaterials biofunctionalization signal amplification bioanalysis biological recognition

Inclusion Behavior of Dimer β-CyclodextrinBridged with Aspartic Acid Derivative

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Chinese Chemical Letters 2001年第2期12卷 119-120页

作者： Le Xin SONG Coordination Chemistry Institute & state Key laboratory of Coordination Chemistry, nanjing university, {3. 210093 nanjing Univ Inst Coordinat Chem Nanjing 210093 Peoples R China nanjing Univ State Key Lab Coordinat Chem Nanjing 210093 Peoples R China

The (β-cyclodextrin (CD) dimer bridged with aspartic acid (ASP) derivative, FITC-ASP(NH-(-CD)2 (Host, FITC=fluorescein-4-isothiocyanate), was synthesized. Fluorescence polarization study showed that the novel host formed an inclusion compound, [FITC-ASP(NH-(-CD)2]ATA, for which Kd was determined to be 5.0×10-6 mol/L by Beacon 2000 Analyzer, when ATA (Guest) = Adm-Trp-Arg-Arg-NH2 (Adm = 1-adamantanecarboxylic acid, Trp = tryptophan, Arg = arginine), where Kd is the dissociation constant in aqueous solution at 298 K.

关键词： β-Cyclodextrin amino acid peptide inclusion compound fluorescence polarization.

SCALING LAW OF IRREGULAR EUTECTIC IN DIRECTIONAL SOLIDIFICATION

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Acta Metallurgica Sinica(English Letters) 1993年第5期6卷 283-290页

作者： LIU Junming LIU Zhiguo WU Zhuangchun nanjing university,China LIU Junming,lecturer,national laboratory of Solid {3. Microstructuros,nanjing university,nanjing 210008,China

The profile features of the solidifying interface of the irregular Al-Si eutectic during directional solidification are studied by numerically solving a nonlinear coupling equation of directional solidification of this *** critical splitting point of the solidifying interface coincides with the maximum value of its position and corresponds to the marginal stability *** is presented that the interlamellar spacing for local regular structure selects the critical splitting state of the α(Al)-liquid interface as its operating point,and the oper- ating point of the average interflake spacing for the irregular structure is the critical splitting state of the β(Si)-liquid *** scaling laws derived,which have the more general forms than the classic Jackson-Hunt scaling,gained support from the experimental results.

关键词： irregular Al-Si eutectic directional solidification scaling law

HARMONIC RESPONSE AND FLUX CREEP OF SUPERCONDUCTOR

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南京大学学报(自然科学) 1999年第5期 634-635页

作者： Ding Shiying (Department of Physics,national laboratory of Solid state {3.,nanjing university, Center for Advance Studies in Science and Technology of {3.,210093.nanjing, PRC)

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关键词： ac susceptibility superconductivity harmonics

Expression of Recombinant Chinese Bovine Enterokinase Catalytic Subunit in P. pastoris and Its Purification and Characterization

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Acta Biochimica et Biophysica Sinica 2004年第7期36卷 513-517页

作者： Lei FANG, Qi-Ming SUN, and Zi-Chun HUA*The state Key laboratory of Pharmaceutical Biotechnology, nanjing university, {3. 210093. China The state Key laboratory of Pharmaceutical Biotechnology Nanjing University Nanjing 210093 China

Enterokinase is a tool protease widely utilized in the cleavage of recombinant fusion proteins. cDNA encoding the catalytic subunit of Chinese bovine enterokinase (EKL) was amplified by PCR and then fused to the 3. end of prepro secretion signal peptide gene of α-mating factor from Saccharomyces cerevisiae to get the α-MF signal-EKL-His6 encoding gene by PCR. Then the whole coding sequence was cloned into the integrative plasmid pAO815 under the control of a methanol-inducible promoter and transformed GS115 methylotrophic strain of Pichia pastoris. Secreted expression of recombinant EKL-His6 was attained by methanol induction and its molecular weight is 43.kD. Because of the existence of His6-tag, EKL-His6 was easily purified from P. pastoris fermentation supernatant by using Ni2+ affinity chromatography and the yield is 5.4 mg per liter of fermentation culture. This purified EKL-His6 demonstrates excellent cleavage activity towards fusion protein containing EK cleavage site.

关键词： recombinant enterokinase secreted expression Ni2+ affinity chromatography fusion protein cleavage