检索结果-南通市图书馆

成品输液物理稳定性与相容性检查指标专家共识

在线全文

学校读者我要写书评

暂无评论

医药导报 2024年第2期43卷 149-155页

作者：安徽省药师协会静脉用药调配专业委员会中国科学技术大学附属第一医院/安徽省立医院安徽中医药大学第一附属医院童彤刘圣唐丽琴丁海文吴健顾菲菲不详中国科学技术大学附属第一医院/安徽省立医院安徽中医药大学第一附属医院

成品输液是通过无菌操作技术将一种或数种静脉药物进行混合调配,可供临床直接用于患者静脉输注的药液。静脉药物在溶解和(或)稀释调配、保存和使用过程中,由于多种因素的影响,可能导致成品输液稳定性和相容性发生变化,存在用药安全风险... 详细信息

成品输液是通过无菌操作技术将一种或数种静脉药物进行混合调配,可供临床直接用于患者静脉输注的药液。静脉药物在溶解和(或)稀释调配、保存和使用过程中,由于多种因素的影响,可能导致成品输液稳定性和相容性发生变化,存在用药安全风险。目前没有统一的成品输液稳定性与相容性控制技术及质量标准或相应的指导性文件,为提高成品输液质量,保障静脉用药安全性和有效性,降低患者用药风险,安徽省药师协会静脉用药调配专业委员会在试验研究、文献复习、专家咨询的基础上,结合国内静脉药物调配实践,编写《成品输液物理稳定性与相容性检查指标专家共识》,旨在为成品输液质量检查和研究提供科学、简单、快速、可行的检查方法及标准。

关键词：成品输液稳定性相容性专家共识

Hyaluronic acid fragments evoke Kupffer cells via TLR4 signaling pathway

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2009年第2期52卷 147-154页

Kupffer cells, expressing toll-like receptor 4 (TLR4), play a central role in hepatic ischemia/reperfusion (I/R) injury. Hyaluronic acid (HA) fragments, degradative products of high-molecular-weight HA (HMW-HA), acquire the ability to activate immune cells under inflammatory conditions. Here we inves- tigated whether HA fragments could activate Kupffer cells and analyzed the underlying mechanism. Kupffer cells were isolated from wild-type mice (WT, C3H/HeN) and TLR4 mutant mice (C3H/HeJ) and HA fragments were produced by the methods of enzyme digestion and chromatography. Then Kupffer cells were stimulated by HA fragments or other control stimuli. The activation of Kupffer cells was estimated as the release of pro-inflammatory cytokines. The activation of p38 MAPK pathway of Kupffer cells was checked and blocking experiments were done as well. The results indicated that HA fragments acquired the ability to activate Kupffer cells in vitro, which was TLR4 dependent and not due to contamination of lipopolysaccharide. Experiments of p38 MAPK kinase inhibition by SB-203580 verified p38 MAPK was required in HA fragments induced Kupffer cells activation. This suggests that HA fragments, degradative products of one of the major glycosaminoglycans of the extracellular matrix, play critical roles in Kupffer cell activation mediated by TLR4 signaling pathway, which is, at least partially, de- pendent on p38 MAPK activation.

关键词： Kupffer cells hyaluronic acid fragments toll-like receptor 4 p38 MAPK lipoplysaccharide reperfusion injury liver

RNA interference by expression of short hairpin RNAs suppresses bcl-xL gene expression in nasopharyngeal carcinoma cells

在线全文

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2005年第2期26卷 228-234页

作者： Fang LIU~2,Cheng-wei HE,Yue-fei ZHANG~4,Ke-yuan ZHOU~3~2Institute of Biochemistry and Molecular Biology ~4Department of Otorhinolaryngology,The First affiliated hospital,Guangdong MedicalCollege,Zhanjiang 524023,China Institute of Biochemistry and Molecular Biology Guangdong Medical College Zhanjiang China Department of Otorhinolaryngology The First Affiliated Hospital Guangdong Medical College Zhanjiang China

Aim:To evaluate a new plasmid mediated RNA interference(RNAi)system andinvestigate whether knock-down of bcl-xL by short hairpin RNA(shRNA)caninduce apoptosis of human nasopharyngeal carcinoma(NPC)cell line CNE-2Z ***:The plasmid containing mU6 promoter was subcloned to yield thepmU6 plasmid,recombinant plasmid expressing shRNA targeting bcl-xL gene wasdesigned and constructed,and were co-transfected cells with green fluorescenceprotein expressing *** cytometry was used to evaluate transfectionefficiency,and RT-PCR and Western blot were applied to analyze bcl-xL mRNAand protein levels,***:The shRNA expressed by the recombi-nant plasmid efficiently suppressed bcl-xL gene expression and induced apoptosisof NPC cells in ***:The recombinant plasmid can sufficientlymediate RNAi in CNE-2Z cells,and knock-down of the bcl-xL expression by shRNAsignificantly induced apoptosis in CNE-2Z *** results suggest this newsystem,mediated RNAi can be used as a tool for the study of gene function andgene therapy.

关键词： RNAi plasmid gene transfection short hairpin RNA bcl-xL apoptosis nasopharyngeal carcinoma cells

Risk factors for delayed graft function in death cardiac donor renal transplants:A Preliminary Report

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Risk factors for delayed graft function in death cardiac don...

Preliminary Results of transplantation with kidneys donated after cardio-circulatory death

2012中国器官移植大会

作者： Mingjie Shao,Qifa Ye,Yingzi Ming Transplant Center of Xiangya 3rd hospital

【Background】Delayed graft function(DGF)is not uncommon in kidney transplants from cardiac death(DCD)*** is associated with various *** of center-specific risk factors may help to reduce the incidence of DGF and improve transplantation *** aim of this study is to define risk factors of DGF after renal transplantation.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Preliminary Results of transplantation with kidneys donated ...

Identification of in vivo induced protein antigens of Salmonella enterica serovar Typhi during human infection

2012中国器官移植大会

作者： Yingzi Ming,Mingjie Shao,Qifa Ye Transplant Center of Xiangya 3rd hospital

【Introductions】Donation after cardiac death(DCD),formerly non-heart-beating donation has been developed in our country *** aim of our study was to report clinical outcomes of kidney transplantation from DCD donors in China,and to investigate its feasibility to expand the organ donor pool.【Methods】From March 2010 to June 2012,56 kidneys acquired from DCD were transplanted into 56 ***

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2009年第10期52卷 942-948页

作者： HU Yong1,2, CONG YanGuang1, LI Shu1, RAO XianCai1, WANG Gang3 & HU FuQuan1 1 Department of Microbiology, Third Military Medical University, Chongqing 400038, China 2 Department of Biotechnology, Chongqing University of Technology, Chongqing 400050, China 3 Department of Clinical Laboratory, The 3rd hospital of People’s Liberation Army, Baoji 721004, China Department of Microbiology Third Military Medical University Chongqing China Department of Biotechnology Chongqing University of Technology Chongqing China Department of Clinical Laboratory The 3rd Hospital of People’s Liberation Army Baoji China

During infectious disease episodes, pathogens express distinct subsets of virulence factors which allow them to adapt to different environments. Hence, genes that are expressed or upregulated in vivo are implicated in pathogenesis. We used in vivo induced antigen technology (IVIAT) to identify antigens which are expressed during infection with Salmonella enterica serovar Typhi. We identified 7 in vivo induced (IVI) antigens, which included BcfD (a fimbrial structural subunit), GrxC (a glutaredoxin 3), SapB (an ABC-type transport system), T3663 (an ABC-type uncharacterized transport system), T3816 (a putative rhodanese-related sulfurtransferase), T1497 (a probable TonB-dependent receptor) and T3689 (unknown function). Of the 7 identified antigens, 5 antigens had no cross-immunoreactivity in adsorbed control sera from healthy subjects. These 5 included BcfD, GrxC, SapB, T3663 and T3689. Antigens identified in this study are potential targets for drug and vaccine development and may be utilized as diagnostic agents.

关键词： Salmonella enterica serovar Typhi in vivo induced antigen technology (IVIAT) virulence

Hepatocyte-like cells from directed differentiation of mouse bone marrow cells in vitro

在线全文

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2005年第4期26卷 469-476页

作者： Xiao-lei SHI~2 Yu-dong QIU~2 Qiang LI Ting XIE~2 Zhang-hua ZHU~2 Lei-lei CHEN~3 Lei LI~3 Yi-tao DING~(2,4) 2 Department of Hepatobiliary Surgery 3 Scientific Research Department,affiliated Drum Tower hospital,Medical College of Nanjing University,Nanjing 210008,China Department of Hepatobiliary Surgery Affiliated Drum Tower Hospital Medical College of Nanjing University Nanjing China Scientific Research Department Affiliated Drum Tower Hospital Medical College of Nanjing University Nanjing China

Aim:To design the effective directed differentiation medium to differentiate bonemarrow cells into hepatocyte-like ***:Bone marrow cells were cul-tured in the directed differentiation media including fibroblast growth factor-4(FGF-4)and oncostatin M(OSM).Hepatocyte-like cells from directed differentia-tion of bone marrow cells were identified through cell morphology,RNA expres-sions by reverse transcriptase-polymerase chain reaction(RT-PCR),protein ex-pressions by Western blot,and hepatocellular synthesis and metabolism func-tions by albumin ELISA,Periodic acid-Shift staining and urea ***:Some epithelial-like cells or polygonal cells appeared and increased in the courseof the cell directed *** nucleur factor-3[3(HNF-3β),albu-min(ALB),cytokeratin 18(CK18),transthyretin(TTR),glucose-6-phosphate(G-6-Pase),and tyrosine aminotransferase(TAT)mRNA were expressed in the courseof the directed *** directed differentiated cells on d 21 expressedHNF-3β,ALB,and CK18 *** directed differentiated cells producedalbumin and synthesized urea in a time-dependent *** could also syn-thesize ***:Our differentiation media,including FGF-4 andOSM,are effective to differentiate bone marrow cells into hepatocyte-like cells,which could be used for hepatocyte resources for bioartificial liver or hepatocytetransplantation.

关键词： bone marrow cells hepatocytes cell differen tiation stern cells

Sodium butyrate-induced death-associated protein kinase expression promote Raji cell morphological change and apoptosis by reducing FAK protein levels

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2007年第11期28卷 1783-1790页

作者： Hai-tao ZHANG~(2,5),Zhe-ling FENG~3,Jun WU~4,Ya-jun WANG~2,Xia GUO~2,Nian-ci LIANG~2,Zhen-yu ZHU~(3,5),Jian-quan MA~3~2 Institute of Biochemistry and Molecular Biology Guangdong Medical College,Zhanjiang 524023,China ~3 Department of Biochemistry andMolecular Biology.Sums Medical College of Sums University Guangzhou 510089,China ~4 affiliated hospital of Guangdong MedicalCollege,Zhanjiang 524023,China Institute of Biochemistry and Molecular Biology Guangdong Medical College Zhanjiang China Department of Biochemistry and Molecular Biology Sums Medical College of Sums University Guangzhou China affiliated hospital of Guangdong Medical College Zhanjiang China

Aim:To investigate the role of death-associated protein kinase (DAPK) on theapoptosis of Raji cells induced by sodium ***:The apoptosis ofRaji cells were induced by sodium butyrate for 2,4,6,8,and 10 ***,theRaji cells were inhibited to adhere on culture flask by *** viabilitywas detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidemethod and the cell apoptosis percentage was estimated by flow *** focal adhesion kinase (FAK) expression were measured by Western *** sequence on the C-terminal of DAPK,which can suppress the function ofDAPK,was tranfected into the Raji cells to investigate whether the C-terminal ofDAPK could inhibit the apoptosis of Raji cells induced by sodium ***:After being treated with sodium butyrate,the Raji cells expressed DAPK anddisplayed many protrusions to adhere onto the culture *** Raji cells weresusceptive to apoptosis when they were inhibited adhesion by *** time,the cell viability decreased,the cell apoptosis percentage increased andthe protein levels of total FAK were *** Raji cells,which were trans-fected with the coding region on the C-terminal of DAPK,sustained apoptosisand the FAK protein level when treated with sodium ***:So-dium butyrate induced DAPK *** caused the Raji cells to display manyprotrusions all around the cells and adhere onto the culture *** expres-sion prompted apoptosis by reducing the FAK protein level in sodium butyrate-induced Raji cells.

关键词： Raji cells death-associated protein kinase apoptosis sodium butyrate focal adhesion kinase

Inhibitory effect of dendroaspis natriuretic peptide on spontaneous contraction in gastric antral circular smooth muscles of guinea pigs

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2007年第11期28卷 1797-1802页

作者： Hui-shu GUO~(2,5),Zheng-xu CAI~3,Tai-hua WU~2,Jing XU~3,Yang QIU~2,Wen-xie XU~4~2 Centralab and ~3 Department of Neurology,The Fist affiliated hospital of Dalian Medical University,Dalian 116011,China ~4 Department ofPhysiology Shanghai Jiao Tong University Medical College,Shanghai 200240,China Centralab The Fist Affiliated Hospital of Dalian Medical University Dalian China Department of Neurology The Fist Affiliated Hospital of Dalian Medical University Dalian China Department of Physiology Shanghai Jiao Tong University Medical College Shanghai China

Aim:To determine whether the natriuretic peptide receptor (NPR) is present in thestomach of guinea pigs and to investigate the effect of dendroaspis natriureticpeptide (DNP) on the gastric motility of guinea pigs and its ***:The distribution of the NPR was analyzed by *** spon-taneous contraction of gastric antral circular muscles of guinea pigs was recordedby a 4-channel *** whole cell patch-clamp technique was intro-duced to record calcium-activated potassium currents in the gastric myocytesisolated by ***:The NPR existed in the gastric fundus,gastricbody,and gastric antrum of guinea pigs,and its density was largest in the *** inhibited spontaneous contraction and exhibited a *** DNP-induced inhibition was diminished by LY83583 (a guanylatecyclase inhibitor) and was potentiated by zaprinast (a cGMP-sensitivephosphoesterase inhibitor).The inhibitory effect of DNP on spontaneous con-traction was also inhibited by tetraethylammonium (a non-selective potassiumchannel blocker);10 nmol/L DNP increased the calcium-activated potassium cur-rents in the gastric circular myocytes of guinea ***:The NPR ismost common in the gastric antrum of guinea *** significantly inhibitsgastric motility in the gastric antrum of guinea *** inhibitory effect occursvia a cGMP-dependent pathway,and a calcium-activated potassium channel maybe also involved in the relaxation induced by DNP in gastric antral circular smoothmuscles.

关键词： dendroaspis natriuretic peptide motility natriuretic peptide receptor spontaneous contraction