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Reactions of Maruca Resistant Transgenic Cowpea to Cowpea Aphid-Borne Mosaic Virus

Agricultural Sciences 2024年第7期15卷 742-753页

作者： Mounyratou Rabo Moustapha Ouédraogo Orokia Coulibaly Assita Traoré-Barro Salimata Traoré teyioue benoit joseph batieno Chantal Kaboré-Zoungrana Aboubacar Toguyeni Oumar Traoré Laboratoire National de Bioscurit Agence Nationale de Bioscurit (ANB) Ouagadougou Burkina Faso Institut de lEnvironnement et Recherches Agricoles Dpartement Productions Vgtales Ouagadougou Burkina Faso Laboratoire dEtudes et de Recherches sur les Ressources Naturelles et les Sciences de lEnvironnement (LERNSE) Universit Nazi BONI Bobo-Dioulasso Burkina Faso Laboratoire De Gntique et Amlioration Des Plantes (LAGAMEP) Institut de lEnvironnement et Recherches Agricoles Ouagadougou Burkina Faso Laboratoire De Protection et Dfense Des Cultures (LAPRODEC) Institut de lEnvironnement et Recherches Agricoles Ouagadougou Burkina Faso

Cowpea (Vigna unguiculata L. [Walp.]) in one of the main grain legumes contributing to food security and poverty alleviation in Sub-Saharan Africa. To control the highly damaging legume pod borer Maruca vitrata F., transgenic cowpea lines expressing the insecticidal Cry1Ab Bt protein were developed. In this study, we evaluated the impact of Cry1Ab transgene expression on the susceptibility of four cowpea lines (named IT97K-T, IT98K-T, Gourgou-T and Nafi-T) and their respective non-transgenic near isogenic lines (IT97K, IT98K, Gourgou and Nafi) to Cowpea aphid-borne mosaic virus (CABMV) in greenhouse conditions. In a preliminary quality control test by enzyme-linked immunosorbent assay, the presence of Cry1Ab protein in transgenic seed lots ranged from 59% to 72%, with no significant differences among the lines (χ2 = 3.26;p = 0.35). Upon virus inoculation, all cowpea lines exhibited mosaic symptoms with similar severity between 7- and 11-day post-inoculation. No significant differences were observed in symptom severity. Significant differences were found between cowpea lines for time of symptom onset, virus accumulation in plants and days to 50% flowering. However, while comparing pairs of transgenic lines and corresponding non-transgenic lines, virus accumulation showed not significant differences whatever the pair. Time of symptom onset and days to 50% flowering did not also differ significantly between pairs of cowpea lines except Nafi/Nafi-T in which transgenic Nafi-T showed earlier symptoms (7.4 ± 0.7 vs. 8.9 ± 0.8 days post-inoculation) and shorter flowering time (37.3 ± 0.6 vs. 42 ± 1.7 days after sowing). Overall, these findings improve our understanding of the effects of Cry1Ab gene mediated genetic modification on cowpea infection by Cowpea aphid-borne mosaic virus, with potential implications for environmental safety assessment.

关键词： Cowpea Cry1Ab Cowpea Aphid-Borne Mosaic Virus Environmental Safety

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Reactions of Maruca Resistant Transgenic Cowpea to Cowpea Aphid -Borne Mosaic Virus

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Agricultural Sciences（农业科学） 2024年第7期 742页

作者： Mounyratou Rabo Moustapha Ouédraogo Orokia Coulibaly Assita Traoré-Barro Salimata Traoré teyioue benoit joseph batieno Chantal Kaboré-Zoungrana Aboubacar Toguyeni Oumar Traoré

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Screen House Assessment of Cowpea [(Vigna unguiculata (L.)] Genotypes for Drought Tolerance Using Selection Indices

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Agricultural Sciences 2023年第4期14卷 457-473页

作者： Sekou Armand Sanogo Sory Diallo teyioue benoit joseph batieno Adejumobi Idris Ishola Nerbéwendé Sawadogo Daniel Nyadanu Department of Horticulture Faculty of Agriculture Kwame Nkrumah University of Science and Technology (KNUST) Kumasi Ghana Institut d’Economie Rurale (IER) Bamako Mali Institut de l’Environnement et de la Recherche Agricole (INERA Kamboinsé/CNRST) Ouagadougou Burkina Faso Equipe Génétique et Amélioration des Plantes Laboratoire Biosciences Ecole Doctorale Sciences et Technologies Université Joseph KI-ZERBO Ouagadougou Burkina Faso

Cowpea [Vigna unguiculata (L.)] is one of the most important arid legumes cultivated for pulse and forage production. Drought is one of the most damageable constraints to crop production impacting negatively food security. The potential of cowpea to address food security is well established. However, not much is known about the base index selection method in breeding cowpea for drought tolerance, which is important for yields. Consequently, the present study has been conducted to: 1) evaluate the yield performance of cowpea genotypes under artificial drought and well-watered condition, 2) ranke genotype performance using selection indices, and 3) assess relationship between agronomic traits and yield. The experiment was the 2 watering conditions laid out in a Randomized Complete Block Design (RCBD) with three replications. The experiment was carried out in pots under screen house at the Department of Horticulture at KNUST. The result showed that KPR1-96-73, Simbo, CZ06-4-16, Wilibaly and Agyenkwa were high yielding in well-water condition while Ghana Shoba, Sangaraka, Nketewade, Ghana Shoni and Korobalen were high yielding genotypes in water stress condition. The average yield reduction was 60.6% and 16% for grain and fodder yield respectively. The biplot displays revealed four groups among the genotypes tested which were based on their yielding capacity and drought tolerance. In cluster B high yielding and drought tolerant genotypes were identified, high yielding and drought susceptible have been identified in cluster A, low yielding and drought tolerant in cluster D, and lastly low yielding and drought susceptible in cluster C. Genotypes in cluster B were best due to the fact that it combines high yield and tolerance to drought. They were Ghana Shoni, Nketewade, Sangaraka and Ghana Shoba. These genotypes might be suitably employed in further drought tolerance breeding programs of cowpea. Significant relationships were observed between agronomic trait and yields un

关键词： Cowpea [Vigna unguiculata (L.)] Drought Selection Index

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Protein Profiles of Pod Borer Maruca Resistant Transgenic Cowpea

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American Journal of Plant Sciences 2023年第12期14卷 1453-1463页

作者： Mounyratou Rabo teyioue benoit joseph batieno Assita Traoré-Barro Salimata Traoré Orokia Coulibaly Aboubacar Toguyeni Chantal Kaboré-Zoungrana Oumar Traoré Laboratoire National de Biosécurité Agence Nationale de Biosécurité (ANB) Ouagadougou Burkina Faso Institut de l’Environnement et Recherches Agricoles Département Productions Végétales Ouagadougou Burkina Faso Laboratoire d’Etudes et de Recherches sur les Ressources Naturelles et les Sciences de l’Environnement (LERNSE) Université Nazi BONI Bobo-Dioulasso Burkina Faso Laboratoire de Génétique et amélioration des plantes (LAGAMEP) Institut de l’Environnement et Recherches Agricoles Ouagadougou Burkina Faso Laboratoire de protection et défense des cultures (LAPRODEC) Institut de l’Environnement et Recherches Agricoles Ouagadougou Burkina Faso

The grain legume cowpea Vigna unguiculata (L.) Walp. is a major protein source used for food and feed in Sub-Saharan Africa. The crop is affected by the pod borer Maruca vitrata against which transgenic lines were developed as part of the genetic control approach. This study aimed to assess the protein profiles in seeds and leaves of transgenic cowpea lines and their non-transgenic near-isogenic counterparts. Crude protein content was determined by the Kjeldahl method, and soluble proteins were quantified using Bradford dye binding assay. The average crude protein content ranged between 21.61% and 26.58% in the seeds and between 10.86% and 17.90% in the leaves. Total solubility varied between 13.03% and 20.64%. Osborne’s protein fractions contents in the seeds were 52.41% - 69.52% (albumin), 4.62% - 7.19% (globulin), 7.95% - 11.40% (glutelin) and 3% - 4% (prolamin). In any case, protein content differed significantly between cowpea genotypes but not between pairs of transgenic/non-transgenic lines. Insecticidal Cry1Ab protein expressed by transgenic lines was only detected in the albumin and globulin fractions. Altogether, these findings enhance our understanding of the effects of genetic modification on cowpea protein content and composition, with potential implications for nutritional and safety assessments.

关键词： Cowpea Protein Cry1Ab Protein Fractions

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Screen House Assessment of Cowpea [(Vigna unguiculata (L.)] Genotypes for Drought Tolerance Using Selection Indices

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Agricultural Sciences（农业科学） 2023年第4期

作者： Sekou Armand Sanogo Sory Diallo teyioue benoit joseph batieno Adejumobi Idris Ishola NerbéwendéSawadogo Daniel Nyadanu

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Protein Profiles of Pod Borer Maruca Resistant Transgenic Cowpea

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American Journal of Plant Sciences（美国植物学期刊） 2023年第12期

作者： Mounyratou Rabo teyioue benoit joseph batieno Assita Traoré-Barro Salimata Traoré Orokia Coulibaly Aboubacar Toguyeni Chantal Kaboré-Zoungrana Oumar Traoré

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Selection of Cowpea [(Vigna unguiculata (L.) WALP] Genotypes for Drought Tolerance Using Selection Indices

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Agricultural Sciences 2023年第3期14卷 384-397页

作者： Sekou Armand Sanogo Sory Diallo Daniel Nyadanu Téyioué benoit joseph batieno Nerbéwendé Sawadogo Department of Horticulture Faculty of Agriculture Kwame Nkrumah University of Science and Technology (KNUST) Kumasi Ghana Institut D’Economie Rurale (IER) Bamako Mali Institut de l’Environnement et de la Recherche Agricole (INERA Kamboinsé/CNRST) Ouagadougou Burkina Faso Equipe Génétique et Amélioration des Plantes Laboratoire Biosciences Ecole Doctorale Sciences et Technologies Université Joseph KI-ZERBO Ouagadougou Burkina Faso

Cowpea [(Vigna unguiculata (L.)] is one of the most important arid legumes cultivated for pulse and forage production. However, in cowpea, not much is known about the base index selection method in breeding for drought tolerance. Consequently, the present study has been conducted to: 1) evaluate the yield performance of cowpea genotypes under artificial drought and well-watered condition;2) develop a base index using multiple traits for ranking genotype performance. The experiment was a 25 × 2 factorial laid out in a Randomized Complete Block Design (RCBD) with three replications. The experiment was carried out in the screen house at the Department of Horticulture at KNUST. The result showed that KPR1-96-73, Simbo, CZ06-4-16, Wilibaly and Agyenkwa were high yielding in well-water condition while Ghana Shoba, Sangaraka, NKetewade, Ghana-Shoni and Korobalen were high yielding genotypes in water stress condition. The average yield reduction was 60.6% for grain respectively. The biplot displays revealed four groups among the genotypes tested which was based on their yielding capacity and drought tolerance. In cluster B high yielding and drought tolerant genotypes were identified, high yielding and drought susceptible have been identified in cluster A, low yielding and drought tolerant in cluster D, and lastly low yielding and drought susceptible in cluster C. Genotypes in cluster B, were the best due to the fact that it combines high yield and tolerance to drought. They were Ghana Shoni, Nketewade, Sangaraka and Ghana shoba. These genotypes might be suitably employed in further drought tolerance breeding program of cowpea.

关键词： Cowpea [(Vigna unguiculata (L.)] Post Flowering Drought Drought Tolerance Index

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Redesigning crop varieties to win the race between climate change and food security

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Molecular Plant 2023年第10期16卷 1590-1611页

作者： Kevin V.Pixley Jill E.Cairns Santiago Lopez-Ridaura Chris O.Ojiewo Maryam Abba Dawud Inoussa Drabo Taye Mindaye Baloua Nebie Godfrey Asea Biswanath Das Happy Daudi Haile Desmae benoit joseph batieno Ousmane Boukar Clare T.M.Mukankusi Stanley T.Nkalubo Sarah J.Hearne Kanwarpal S.Dhugga Harish Gandhi Sieglinde Snapp Ernesto Adair Zepeda-Villarreal International Maize and Wheat Improvement Center(CIMMYT) TexcocoMexico International Maize and Wheat Improvement Center(CIMMYT) HarareZimbabwe International Maize and Wheat Improvement Center(CIMMYT) NairobiKenya Lake Chad Research Institute(LCRI) MaidiguriNigeria International Maize and Wheat Improvement Center(CIMMYT) DakarSenegal Ethiopian Institute of Agricultural Research(EIAR) Addis AbabaEthiopia National Agricultural Research Organization(NARO) KampalaUganda Tanzania Agricultural Research Institute(TARI) NaliendeleTanzania Institut de l'Environnement et de Recherches Agricoles(INERA) OuagadougouBurkina Faso International Institute of Tropicl Agriculture(IITA) KanoNigeria Alliance of Bioversity International and CIAT KampalaUganda

Climate change poses daunting challenges to agricultural production and food *** temperatures,shifting weather patterns,and more frequent extreme events have already demonstrated their effects on local,regional,and global agricultural *** varieties that withstand climate-related stresses and are suitable for cultivation in innovative cropping systems will be crucial to maximize risk avoidance,productivity,and profitability under climate-changed *** surveyed 588 expert stakeholders to predict current and novel traits that may be essential for future pearl millet,sorghum,maize,groundnut,cowpea,and common bean varieties,particularly in sub-Saharan *** then review the current progress and prospects for breeding three prioritized future-essential traits for each of these *** predict that most current breeding priorities will remain important,but that rates of genetic gain must increase to keep pace with climate challenges and consumer ***,the predicted future-essential traits include innovative breeding targets that must also be prioritized;for example,(1)optimized rhizosphere microbiome,with benefits for P,N,and water use efficiency,(2)optimized performance across or in specific cropping systems,(3)lower nighttime respiration,(4)improved stover quality,and(5)increased early *** further discuss cutting-edge tools and approaches to discover,validate,and incorporate novel genetic diversity from exotic germplasm into breeding populations with unprecedented precision,accuracy,and *** conclude that the greatest challenge to developing crop varieties to win the race between climate change and food security might be our innovativeness in defining and boldness to breed for the traits of tomorrow.

关键词： climate change food security crop breeding cropping systems expert survey sub-Saharan Africa

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Selection of Cowpea [(Vigna unguiculata (L.) WALP] Genotypes for Drought Tolerance Using Selection Indices

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Agricultural Sciences（农业科学） 2023年第3期

作者： Sekou Armand Sanogo Sory Diallo Daniel Nyadanu Téyioué benoit joseph batieno Nerbéwendé Sawadogo

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