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Simultaneous detection of the shuttling motion of liquid metal droplets in channels under alternating pressure and capacitive sensor signals

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Microsystems & Nanoengineering 2024年第2期10卷 83-90页

作者： Shinji Bono Ryotaro Nakai Satoshi konishi Research Organization of Science and Technology Ritsumeikan UniversityShigaJapan Ritsumeikan Advanced Research Academy KyotoJapan Ritsumeikan Global Innovation Research Organization Ritsumeikan UniversityShigaJapan Graduate School of Science and Engineering Ritsumeikan UniversityShigaJapan

Implementing a signal-switching mechanism for the selective use of integrated sensors and actuators plays a crucial role in streamlining the functionality of miniaturized ***,a liquid metal droplet(LMD)-based signal-switching mechanism is introduced to achieve such *** modulation with a 100-μm spatial resolution enabled precise control of the position of the LMDs within a *** integrating the channel with asymmetrically structured electrodes,the effect of the shuttle-like movement of LMD on the temporal changes in the overall capacitance was ***,analysis of the capacitive peaks revealed the directional movement of the LMDs,enabling estimation of the position of the LMDs without direct *** addition,we achieved successful signal extraction from the capacitive sensor that was linked to the activated electrodes,thereby enabling selective data *** proposed signal-switching mechanism method achieved a detection accuracy of~0.1 *** sensor’s ability to simultaneously detect the LMD position and generate a signal underscores its significant potential for multiplexing in multisensing systems,particularly in concealed environments,such as in vivo settings.

关键词： liquid thereby integrating

Persimmon Leaf Flavonols Enhance the Anti-Cancer Effect of Heavy Ion Radiotherapy on Murine Xenograft Tumors

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Journal of Cancer Therapy 2013年第7期4卷 1150-1157页

作者： Kayoko Kawakami Hiroshi Nishida Naoto Tatewaki Kiyomi Eguchi-Kasai Kazunori Anzai Takahiro Eitsuka tetsuya konishi Masao Hirayama Laboratory of Nutritional Biochemistry Niigata University of Pharmacy & Applied Life Sciences Niigata Japan National Institute of Radiological Sciences Chiba Japan

The cell cycle checkpoint system play a pivotal role in the cellular DNA damage response, and the discovery of checkpoint inhibitors is expected to sensitize current cancer therapies. Checkpoint signaling cascades are critically modulated by ATM (ataxia telangiectasia-mutated) and its related molecules. Generally, ATM primarily responds to ionizing irradiation-induced DNA double-strand breaks. Heavy ions from an accelerated carbon ion beam have been used to cure cancer because they are more effective than ionizing irradiation such as X-ray and γ-radiation in terms of biological damage. In a previous study, we demonstrated that a persimmon leaf flavonol (PLF) promoted the cytotoxic effect of chemotherapeutic agents on cancer cells through inhibition of checkpoint activities, especially in the ATM dependent pathway. The present study investigated whether PLF inhibits checkpoint activity during the DNA damage response induced by heavy ion irradiation. Treatment with PLF significantly increased the cytotoxicity of heavy ion irradiation in A549 adenocarcinoma cells. The phosphorylation of checkpoint proteins such as p53, SMC1, and Chk1 was increased by heavy ions. PLF reduced the phosphorylation of checkpoint proteins. Pre-treatment with PLF significantly prevented the decrease of mitotic cells in heavy ion-exposed cells. We further evaluated tumor volume in SCID mice inoculated with human lung adenocarcinoma A549 cells. The combination treatment of PLF and heavy ion resulted in a decrease of tumor volume compared with controls, although PLF itself did not exhibit any effect. These results indicate that PLF inhibits tumor growth through modulation of the DNA damage response. PLF may be useful for clinical application in combination with heavy ion radiotherapy.

关键词： DNA Damage Checkpoint Persimmon Leaf Flavonols Heavy Ion Irradiation Xenograft

Cerebral Oxidative Stress as Mibyo Pathology:Target for Disease Prevention.

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长春中医药大学学报 2008年第5期24卷 599-599页

作者： tetsuya konishi Niigata University Of Pharmacy&Applied Life Sciences(NUPALS)

The Mibyo(sub-healthy condition)was defined in ancient oriental medicine as the physiological condition that is not *** healthy but not *** ideas is also quite important in current preventive *** the traditional orien... 详细信息

关键词：大脑氧化应激疾病预防措施

单细胞凝胶电泳技术检测小鼠成纤维细胞DNA的氧化损伤及修复

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西安交通大学学报（医学版） 2007年第1期28卷 25-27页

作者：陈忻西田浩志小西徹也首都医科大学中医药学院北京100069 日本新泻药科大学

目的观察小鼠成纤维细胞由过氧化氢(H2O2)引起的DNA损伤及其修复,并详细介绍单细胞凝胶电泳技术。方法培养NIH3T3细胞,H2O2造成细胞氧化损伤,单细胞凝胶电泳技术(SCGE,Comet Assay)检测细胞DNA的损伤情况。结果①建立了H2O2致NIH3T3细胞... 详细信息

目的观察小鼠成纤维细胞由过氧化氢(H2O2)引起的DNA损伤及其修复,并详细介绍单细胞凝胶电泳技术。方法培养NIH3T3细胞,H2O2造成细胞氧化损伤,单细胞凝胶电泳技术(SCGE,Comet Assay)检测细胞DNA的损伤情况。结果①建立了H2O2致NIH3T3细胞DNA损伤的分级图谱;②H2O2引起的小鼠成纤维细胞DNA单链断裂与H2O2的浓度呈依赖性关系;③细胞在除去H2O2后15 min已出现明显修复,多数修复可在1 h内完成,但少数修复可能需要较长时间才能完成。结论单细胞凝胶电泳技术是一种简便、敏感的检测DNA氧化损伤的方法。

关键词：单细胞凝胶电泳 DNA单链断裂 DNA氧化损伤 DNA修复

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抗氧化营养素配方对小鼠抗氧化能力的影响

营养学报 2006年第3期28卷 266-268页

作者：龚慕辛王蕾陈忻小西徹也首都医科大学中医药学院北京100013 新潟药科大学

在前期研究中，已知以小鼠建立氧化损伤模型，测定不同组织中脂质过氧化物（TBARS）、羰基蛋白、谷胱甘肽过氧化酶（GPx）活力的变化，发现不同配方营养素的抗氧化作用不同。本研究进一步探讨其分子机制。

关键词：抗氧化营养素不同配方抗氧化能力小鼠谷胱甘肽过氧化酶氧化损伤模型脂质过氧化物抗氧化作用分子机制

人参皂苷Rg1对过氧化氢诱导PC12细胞氧化损伤的保护作用

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上海中医药杂志 2005年第9期39卷 56-58页

作者：王蕾龚慕辛陈忻小西徹也首都医科大学中医药学院北京100013 日本新泻药科大学

观察人参皂苷Rg1(GSRg1)对H2O2诱导的PC12细胞氧化损伤时细胞存活率、DNA及蛋白的影响。GSRg1与PC12细胞共同培养24h,经H2O2处理30min,通过显微镜观察细胞的形态,MTT法测定细胞生存率,用单细胞凝胶电泳法测定DNA单链损伤,ELISA方法测定... 详细信息

观察人参皂苷Rg1(GSRg1)对H2O2诱导的PC12细胞氧化损伤时细胞存活率、DNA及蛋白的影响。GSRg1与PC12细胞共同培养24h,经H2O2处理30min,通过显微镜观察细胞的形态,MTT法测定细胞生存率,用单细胞凝胶电泳法测定DNA单链损伤,ELISA方法测定细胞内的羰基蛋白含量。结果:经H2O2处理后细胞生存率明显降低,细胞内DNA单链损伤及羰基蛋白含量明显升高,并随H2O2浓度的增加而加重。而经GSRg1预培养的细胞生存率明显升高,细胞内DNA单链损伤及羰基蛋白含量明显减轻,其作用呈剂量依赖关系。结论:GSRg1通过降低H2O2对PC12细胞内DNA和蛋白的氧化损伤而起到对神经细胞的保护作用。

关键词：人参皂苷Rgl PC12细胞 DNA氧化损伤羰基蛋白

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生脉散对应激小鼠组织氧化损伤的影响研究

中国病理生理杂志 2007年第6期23卷 1216-1218页

作者：龚慕辛王蕾王谦李玉娟小西徹也首都医科大学中医药学院北京100054 北京中医药大学基础医学院北京100029 日本新潟药科大学应用生命科学部

目的:研究生脉散对应激小鼠组织氧化损伤的预防作用。方法:通过剪除小鼠胡须的方式造成氧化应激模型,以羰基蛋白(carbonyl protein)、硫代巴比妥酸反应物(thiobarbituric acid reactive substance,TBARS)作为衡量氧化损伤的指标对生脉... 详细信息

目的:研究生脉散对应激小鼠组织氧化损伤的预防作用。方法:通过剪除小鼠胡须的方式造成氧化应激模型,以羰基蛋白(carbonyl protein)、硫代巴比妥酸反应物(thiobarbituric acid reactive substance,TBARS)作为衡量氧化损伤的指标对生脉散的抗氧化能力进行评价。结果:应激小鼠自主活动次数显著增高,预先灌服生脉散可抑制这种增高。应激小鼠脑、心、肝、肾组织中的羰基蛋白量的升高和心、肝组织中的TBARS增高,可以被生脉散抑制。结论:生脉散对应激小鼠组织氧化损伤有防治作用。

关键词：生脉散氧化损伤羰基蛋白质硫巴比妥酸反应物

Anoctamin 5 regulates the cell cycle and affects prognosis in gastric cancer

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World Journal of Gastroenterology 2022年第32期28卷 4649-4667页

作者： Tomoyuki Fukami Atsushi Shiozaki Toshiyuki Kosuga Michihiro Kudou Hiroki Shimizu Takuma Ohashi Tomohiro Arita Hirotaka konishi Shuhei Komatsu Takeshi Kubota Hitoshi Fujiwara Kazuma Okamoto Mitsuo Kishimoto Yukiko Morinaga Eiichi konishi Eigo Otsuji Division of Digestive Surgery Department of SurgeryKyoto Prefectural University of MedicineKyoto 602-8566Japan Department of Pathology Kyoto City HospitalKyoto 604-8845Japan Department of Pathology Kyoto Prefectural University of MedicineKyoto 602-8566Japan

BACKGROUND Anoctamin 5(ANO5)/transmembrane protein 16E belongs to the ANO/transmembrane protein 16 anion channel *** comprise a family of plasma membrane proteins that mediate ion transport and phospholipid scrambling and regulate other membrane proteins in numerous cell *** studies have elucidated the roles and mechanisms of ANO5 activation in various cancer ***,it remains unclear whether ANO5 acts as a plasma membrane chloride channel,and its expression and functions in gastric cancer(GC)have not been *** To examine the role of ANO5 in the regulation of tumor progression and clinicopathological significance of its expression in *** Knockdown experiments using ANO5 small interfering RNA were conducted in human GC cell lines,and changes in cell proliferation,cell cycle progression,apoptosis,and cellular movement were *** gene expression profiles of GC cells were investigated following ANO5 silencing by microarray *** staining of ANO5 was performed on 195 primary tumor samples obtained from patients with GC who underwent curative gastrectomy between 2011 and 2013 at our *** Reverse transcription-quantitative polymerase chain reaction(PCR)and western blotting demonstrated high ANO5 mRNA and protein expression,respectively,in NUGC4 and MKN45 *** these cells,ANO5 silencing inhibited cell proliferation and induced *** addition,the knockdown of ANO5 inhibited G1-S phase progression,invasion,and *** results of the microarray analysis revealed changes in the expression levels of several cyclin-associated genes,such as CDKN1A,CDK2/4/6,CCNE2,and E2F1,in ANO5-depleted NUGC4 *** expression of these genes was verified using reverse transcription-quantitative *** staining revealed that high ANO5 expression levels were associated with a poor *** analysis identified high ANO5 expression as an independent prognostic fa

关键词： Anoctamin 5 Gastric cancer Cell cycle G1/S checkpoint Cell proliferation

Design improvement of the conversion mechanism from balloon inflation to bending motion for inflatable film actuators

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微系统与纳米工程(英文) 2023年第3期9卷 159-170页

作者： Y.Hori S.konishi Graduate School of Science and Engineering Ritsumeikan UniversityShigaJapan Department of Mechanical Engineering Ritsumeikan UniversityShigaJapan Ritsumeikan Advanced Research Academy KyotoJapan Ritsumeikan Global Innovation Research Organization KyotoJapan

Various soft actuators have been investigated to overcome the drawbacks of conventional solid machines and explore the applications of soft *** particular,and because they are expected to be applicable in minimally invasive medicine because of their safety,soft inflatable microactuators using an actuation conversion mechanism from balloon inflation to bending motion have been proposed for high-output bending *** microactuators could be applied to create an operation space by safely moving organs and tissues;however,the conversion efficiency could be further *** study aimed to improve conversion efficiency by investigating the design of the conversion *** contact conditions between the inflated balloon and conversion film were examined to improve the contact area for force transmission,with the contact area dependent on the length of the contact arc between the balloon and force conversion mechanism and on the amount of balloon *** addition,surface contact friction between the balloon and film,which affects actuator performance,was also *** generated force of the improved device is 1.21 N at 80 kPa when it bends 10 mm,which is 2.2 times the generated force of the previous *** improved soft inflatable microactuator is expected to assist in performing operations in a limited space,such as in endoscopic or laparoscopic operations.

关键词： film mechanism operations