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Analysis of in vivo absorption of didanosine tablets in male adult dogs by HPLC

Journal of Pharmaceutical Analysis 2012年第1期2卷 29-34页

作者： Patrfícia Severino Heloisa Silva Eliana B. Souto Maria Helena A. Santana teresa Cristina T. Dalla costa Biotechnological Processes Development Laboratory School of Chemical Engineering State University of Campinas-UNICAMP Campinas School of Pharmacy University of Rio Grande do Sul-UFRGS School of Agrarian Science and Veterinary S o Paulo State University-UNESP Jaboticabal Faculty of Health Sciences Fernando Pessoa University (FCS-UFP) Institute of Biotechnology and Bioengineering Centre of Genomics and Biotechnology University of Trás-os-Montes and Alto Douro (CGB-UTAD/IBB)

Didanosine is an effective antiviral drug in untreated and antiretroviral therapy-experienced patients with Human Immunodeficiency Virus (HIV). An automated system using on-line solid extraction and High Performance Liquid Chromatography (HPLC) with ultraviolet (UV) detection was developed and validated for pharmacokinetic analysis of didanosine in dog plasma. Modifications were introduced on a previous methodology for simultaneous analysis of antiretroviral drugs in human plasma. Extraction was carried out on C18 cartridges, with high extraction yield as stationary phase, whereas mobile phase consisted of a mixture of 0.02 M potassium phosphate buffer, acetonitrile (KH2PO4: acetonitrile: 96:4, v/v) and 0.5% (w/v) of heptane sulphonic acid. The pH was adjusted to 6.5 with triethylamine. All samples and standard solutions were chromatographed at 28 1C. For an isocratic run, the fiux was 1.0 mL/min, detection was at 250 nm and injected volume was 20 mL. The method was selective and linear for concentrations between 50 and 5000 ng/mL. Drug stability data ranged from 96% to 98%, and limit of quantification was 25 ng/mL. Extraction yield was up to 95%. Drug stability in dog plasma was kept frozen at 20 1C for one month after three freeze–thaw cycles, and for 24 h after processing in the auto sampler. Assay was successfully applied to measure didanosine concentrations in plasma dogs.

关键词： Didanosine On-line solid phase extraction HPLC Male dogs

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Neutrophile-to-lymphocyte,lymphocyte-to-monocyte,and platelet-tolymphocyte ratios as prognostic and response biomarkers for resectable locally advanced gastric cancer

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World Journal of Gastrointestinal Oncology 2022年第7期14卷 1307-1323页

作者： Tiago Cruz Tomás Ines Eiriz Marina Vitorino Rodrigo Vicente Joao Gramaca Alicia Guadalupe Oliveira Paulo Luz Mafalda Baleiras Ana Sofia Spencer Luísa Leal costa Patrícia Liu Joana Mendonca Magno Dinis teresa Padrao Marisol Correia Goncalo Atalaia Michelle Silva teresa Fiúza Department of Medical Oncology Hospital Professor Doutor Fernando Fonseca EPEAmadora 2720-276Portugal Department of Medical Oncology Centro Hospitalar Barreiro-Montijo EPEBarreiro 2830-003Portugal Department of Medical Oncology Hospital do Espírito Santo deÉvora EPEÉvora 7000-811Portugal Department of Medical Oncology Centro Hospitalar Universitário do Algarve EPEAlgarve 8000-386Portugal Department of Medical Oncology Hospital São Francisco XavierCentro Hospitalar Lisboa Ocidental EPELisboa 1449-005Portugal Department of Medical Oncology Hospital Santo António dos CapuchosCentro Hospital Lisboa Central EPELisboa 1169-050Portugal Department of Medical Oncology Hospital BeatrizÂngeloLoures 2674-514Portugal Department of Medical Oncology Centro Hospitalar de Trás-os-Montes e Alto Douro EPEVila Real 5000-508Portugal Department of Medical Oncology Hospital da Senhora da Oliveira EPEGuimarães 4835-044Portugal Department of Medical Oncology Hospital Garcia de Orta EPEAlmada 2805-267Portugal Department of Medical Oncology Hospital da LuzLisboa 1500-650Portugal Department of Medical Oncology Hospital Distrital de Santarém EPESantarém 2005-177Portugal

BACKGROUND Perioperative fluorouracil plus leucovorin,oxaliplatin,and docetaxel(FLOT)improves prognosis in locally advanced gastric cancer(LAGC).Neutrophil-to-lymphocyte(NLR),lymphocyte-tomonocyte(LMR),and platelet-to-lymphocyte(PLR)ratios are prognostic biomarkers but not predictive *** To assess blood ratios’(NLR,LMR and PLR)potential predictive response to FLOT and survival outcomes in resectable LAGC *** This was a multicentric retrospective study investigating the clinical potential of NLR,LMR,and PLR in resectable LAGC patients,treated with at least one preoperative FLOT cycle,from 12 Portuguese *** were compared through non-parametric Mann-Whitney *** operating characteristic curve analysis defined the cut-off values as:High PLR>141 for progression and>144 for mortality;high LMR>3.56 for T stage regression(TSR).Poisson and Cox regression models the calculated relative risks/hazard ratios,using NLR,pathologic complete response,TSR,and tumor regression grade(TRG)as independent variables,and overall survival(OS)as the dependent *** This study included 295 patients(mean age,63.7 years;59.7% males).NLR was correlated with survival time(r=0.143,P=0.014).PLR was associated with systemic progression during FLOT(P=0.022)and mortality(P=0.013),with high PLR patients having a 2.2-times higher risk of progression[95% confidence interval(CI):0.89-5.26]and 1.5-times higher risk of mortality(95%CI:0.92-2.55).LMR was associated with TSR,and high LMR patients had a 1.4-times higher risk of achieving TSR(95%CI:1.01-1.99).OS benefit was found with TSR(P=0.015)and partial/complete TRG(P<0.001).Patients without TSR and with no evidence of pathological response had 2.1-times(95%CI:1.14-3.96)and 2.8-times(95%CI:1.6-5)higher risk of *** Higher NLR is correlated with longer survival *** LMR patients have a higher risk of decreasing T stage,whereas high PLR patients have higher odds of progressing under FLOT a

关键词： Gastric cancer Perioperative fluorouracil plus leucovorin,oxaliplatin,and docetaxel Neutrophil-to-lymphocyte Lymphocyte-to-monocyte Platelet-to-lymphocyte Tumor regression grade

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Use of Open Source Software for the Development of Web GIS for Accessibility to Tourist Attractions

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Journal of Civil Engineering and Architecture 2013年第4期7卷 472-486页

作者： Maria teresa Francoso Diogenes Cortijo costa Mariana Mello Valin Rogerio Rodrigues Amarante Department of Geotechnics and Transports School of Civil Engineering Architecture and Urban Design University of CampinasSao Paulo 13083-852 Brazil JBS S.A. Corporation Lins 16402-161 Brazil Campinas Town Hall Americana 13468-100 Brazil

Based on the concepts of social inclusion, universal design and accessibility, this paper aims to describe the stages of the development and implementation of a Web GIS （geographic information system for the internet） based on OS （open source） software The Web GIS developed is called ＂accessibility to Campinas tourist attractions＂, which provides information about cultural and natural attractions in Campinas/SP-Brazil, especially for people with disabilities or reduced mobility that prevents access these sites via public and private transportation. The verification of the existence or lack of accessibility equipment at bus stops and parking areas close to tourist attractions relies on current legislation （NBR 9050/1994） that establishes the right to accessibility for disabled people to transportation, local culture and leisure. An application that facilitates this recognition with low operating costs and high performance was developed using the OS software Quantum GIS 1.0.2, Apache 2.2.4, Mapserver 5.0.2, I3GEO 4.0, PostgreSQL 8.3 and PostGIS 1.3.5. This software is able to integrate the management of tourist areas and the advertisement of tourist information regarding accessibility devices at sights recognized as tourist attractions by Campinas city hall.

关键词： Geographic information systems Web GIS transportation tourism accessibility open source software.

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Ecosystem services potential and soil conservation policies with emphasis on degraded pastures in Brazil

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Geography and Sustainability 2024年第4期5卷 660-672页

作者： Adriana Monteiro da costa Maise Soares de Moura Isadora Fonseca Navarro Victor Cordeiro da Silva teresa Cristina TarléPissarra Carlos Alberto Valera Luís Filipe Sanches Fernandes Fernando Antonio Leal Pacheco Federal University of Minas Gerais(UFMG) Avenida Antônio Carlos6620PampulhaBelo HorizonteMG 31270-901Brazil São Paulo State University(Unesp) School of Agricultural and Veterinarian SciencesJaboticabal.Access Way Prof.Paulo Donato Castellanes/nJaboticabal SP 14884–900Brazil Luiz de Queiroz College of Agriculture University of São Paulo(ESALQ)Avenida Pádua Dias235CEP 13418-900PiracicabaSão Paulo StateBrazil Regional Coordination of Environmental Justice Prosecutors for the Paranaíba and Baixo Rio Grande River Basins Rua Coronel Antônio Rios951UberabaMG 38061-150Brazil CITAB–Center for Agro-environmental and Biological Research and Technologies University of Trás-os-Montes and Alto DouroAp.10135001–801 Vila RealPortugal CQVR–Vila Real Chemistry Center University of Trás-os-Montes and Alto DouroAp.10135001-801 Vila RealPortugal Post-Doctorate researcher University of Trás-os-Montes and Alto DouroAp.10135001-801 Vila RealPortugal POLUS-Land Use Policy Group Paulista State University(UNESP)Access Way Prof.Paulo Donato Castellanes/nJaboticabal SP 14884–900Brazil

Different global agendas have discussed the role of soils in mitigating and reducing global problems related to climate change,food security,biodiversity and erosion,among *** this in mind,the aim was to draw up an overview of the main soil agendas in Europe and Brazil,analysing their impact on the establishment of legal frameworks for soil use and *** Brazil,pastures are seen as an important alternative for converting degraded areas into ecosystem *** this end,a literature review was carried out using databases on the Scopus,Web of Science,Science Direct and Scielo platforms,as well as consulting documents from the European Union Missions in Horizon Europe and Brazilian legislation on the *** a pilot river basin,the Potential for Conservation Use(PCU)method was applied,which is used in Brazil to assess the impacts of different uses on the natural capital of soils,serving as a metric to guide public soil conservation *** was concluded that,on the European scene,discussions on soils have taken place in an integrated and organised way,with actions to mobilise different players in *** Brazil,even though there are important legal frameworks,actions are still restricted and disjointed,pointing to the need for progress in soil *** both scenarios,there is a need for scientifically validated metrics at regional scales that can contribute to a harmonised database capable of subsidising the development of efficient public policies to advance soil conservation at a global level.

关键词： EU mission Soil governance Conservation use potential Soil services PronaSolos

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Evaluation of the Use of Inertial Platform for the Generation of Cartography

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Journal of Civil Engineering and Architecture 2014年第9期8卷 1204-1210页

作者： Luis Antonio de Lima Diogenes Cortijo costa Jorge Luiz Alves Trabanco Maria teresa Francoso Technical Department Fototerra Atividades de Aerolevantamento Ltda (Fototerra Aerial Survey Activities Ltd.) San Paulo 03337-000 Brazil Department of Geotechnics and Transportation The State University of Campinas San Paulo 13083-070 Brazil

This study aims to analyze the quality of positioning orthophoto generated through the technique of direct georeferencing using metric digital camera system coupled to the laser system, and both systems were aided inertial navigation platform. For this, we compared the coordinates obtained in 16 control points collected in the field with dual-frequency GNSS （global navigation satellite system） receiver with those obtained in the orthophoto, flight conducted in June 2009 on the campus of the State University of Campinas--UNICAMP, using a medium format digital camera and laser system, with which we obtained images with a spatial resolution of 0.15 m. Taking into account the pattern accuracy cartographic used in Brazil, it is concluded that the products produced have a standard accuracy ＂A＂ to 1：2,000 scale, which represents the best quality level, both for planimetric as for altimetry, and that the procedure reached results consistent with cartographic products in 1：2,000 scale, representing a reduction of steps in the mapping process, necessary for the preparation of cartographic databases with reduction of time for preparation of design basis large civil engineering projects, such as roads, railways, studies of urban road systems, power plants and other infrastructure systems needed to develop the country.

关键词： Orthophotos photogrammetry GNSS.

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Introduction to the special issue on “COVID-19”

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Geoscience Frontiers 2022年第6期13卷 208-209页

作者： Luis F.O.Silva Weijun Li teresa Moreno Department of Civil and Environmental Universidad de la CostaCUC Department of Atmospheric Sciences School of Earth SciencesZhejiang University Institute for Environmental Assessment and Water Research Consejo Superior de Investigaciones Científicas

COVID-19 pandemic is wreaking havoc and bringing the whole world into a standstill in an unprecedented fashion. Joint efforts from multiple sectors to address the various aspects of environment and public health through usage of green technologies have become essential. The application of cleaner and effective technologies can be expanded to management and control, pollution mitigation and valorization of waste. In this Special Issue of Geoscience Frontiers, we present contributions addressing the interactions of COVID-19 in relation to environment and human health.

关键词： bringing expanded COV

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The road to sustainable use and waste management of plastics in Portugal

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Frontiers of Environmental Science & Engineering 2022年第1期16卷 91-106页

作者： Joana C.Prata Ana LPatrício Silva Armando CDuarte teresa Rocha-Santos Centre for Environmental and Marine Studies(CESAM)&Department of Chemistry University of Aveiro3810-193 AveiroPortugal Centre for Environmental and Marine Studies(CESAM)&Department of Biology University of Aveiro3810-193 AveiroPortugal

As a European Union(EU)member,Portugal must comply with reductions in plastic *** Portugal,the 330 items/100 m of beach litter,comprising up to 3.9 million pieces and of which 88%is plastic,is higher than the EU median(149 items/100 m)and must be reduced to 20 items/100 m(94%).Integrative measures are needed to reduce littering and improve plastics’use and disposal under the circular *** this 414 kt of plastic packaging waste,163 kt were declared plastic packaging,140 kt subjected to recycling,and 94 kt to energy *** current recycling rate of plastic packaging(34%)should be improved to reach EU recycling averages(42%)and goals and to provide widespread benefits,considering revenues of 167€/*** a net importer of waste,Portugal could benefit from the valorization of imported *** increased recycling,pyrolysis and gasification could provide short-term alternatives for producing value-added substances from plastic waste,such as hydrogen,consistent with the National Plan of Hydrogen and improving ongoing regulations on single-use *** manuscript provides an integrative view of plastics in Portugal,from use to disposal,providing specific recommendations under the circular economy.

关键词： Single-use plastics Plastic packaging Plastic waste Waste management Waste shipment Lightweight plastic bags

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Dietary approach in the treatment of nonalcoholic fatty liver disease

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World Journal of Hepatology 2015年第24期7卷 2522-2534页

作者： Silvia Marinho Ferolla Luciana costa Silva Maria de Lourdes Abreu Ferrari Aloísio Sales da Cunha Flaviano dos Santos Martins Cláudia Alves Couto teresa Cristina Abreu Ferrari Departamento de Clínica Médica Centro de Pós-GraduacaoFaculdade de MedicinaUniversidade Federal de Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil Departamento de Propedêutica Complementar Faculdade de MedicinaUniversidade Federal de Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil Departamento de Microbiologia Instituto de Ciências BiológicasUniversidade Federal de Minas GeraisBelo Horizonte 31270-901Minas GeraisBrazil

Nonalcoholic fatty liver disease(NAFLD) has been identified as one of the most prevalent chronic liver disease in adults and children populations. NAFLD is usually associated with the metabolic syndrome(MS), which is chiefly related to insulin resistance and its consequences. Insulin resistance has a crucial role in the pathogenesis of hepatic steatosis and potentially nonalcoholic steatohepatitis(NASH). Because of the contemporary epidemics of MS and obesity, the burden of NAFLD is also expected to rise. Unhealthy diets, such as the so-called western diet, are enriched in fructose, trans-fatty acids and saturated fat and seem to be associated with the development of NAFLD. In human studies, certain dietary sugars, particularly fructose, are used as a substrate for lipogenesis leading to hepatic fatty infiltration, inflammation, and possibly fibrosis. Other investigations have shown that fat consumption especially cholesterol and trans/saturated fatty acids are also steatogenic and seem to increase visceral adiposity. The identification of specific dietary components that favor the development of NASH could be important for the management of this disorder. This review focuses on the effects of different dietary approaches to prevent and treat NAFLD emphasizing the macronutrients and energy composition.

关键词： Fatty liver Dietary carbohydrates Dietary fats Die

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Fluorescent probes for the detection of reactive oxygen species in human spermatozoa

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Asian Journal of Andrology 2020年第5期22卷 465-471页

作者： Sara Escada-Rebelo Francisca G Mora Ana P Sousa teresa Almeida-Santos Artur Paiva Joao Ramalho-Santos phD Programme in Experimental Biology and Biomedicine CNC-Center for Neuroscience and Cell BiologyUniversity of CoimbraCoimbra 3004-504Portugal Biology of Reproduction and Stem Cell Group CNC-Center for Neuroscience and Cell BiologyUniversity of CoimbraCoimbra 3004-504Portugal IIIUC-Institute for Interdisciplinary Research Casa Costa AlemaoUniversity of CoimbraCoimbra 3030-789Portugal Reproductive Medicine Unit University Hospitals of CoimbraCoimbra 3004-561Portugal Faculty of Medicine University of CoimbraCoimbra 3000-370Portugal Clinical Pathology Unit University Hospitals of CoimbraCoimbra 3004-561Portugal Department of Life Sciences University of CoimbraCoimbra 3000-456Portugal

Reactive oxygen species(ROS)production is a by-product of mitochondrial activity and is necessary for the acquisition of the capacitated state,a requirement for functional ***,an increase in oxidative stress,due to an abnormal production of ROS,has been shown to be related to loss of sperm function,highlighting the importance of an accurate detection of sperm ROS,given the specific nature of this *** this work,we tested a variety of commercially available fluorescent probes to detect ROS and reactive nitrogen species(RNS)in human sperm,to define their *** both flow cytometry(FC)and fluorescence microscopy(FM),we confirmed that MitoSOX™Red and dihydroethidium(DHE)detect superoxide anion(as determined using antimycin A as a positive control),while DAF-2A detects reactive nitrogen species(namely,nitric oxide).For the first time,we also report that RedoxSensor™Red CC-1,CellROX®Orange Reagent,and MitoPYl seem to be mostly sensitive to hydrogen peroxide,but not ***,mean fluorescence intensity(and not percentage of labeled cells)is the main parameter that can be reproducibly monitored using this type of methodology.

关键词： flow cytometry fluorescent probes human spermatozoa oxidative stress reactive oxygen species

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Damaging effects of gliadin on three-dimensional cell culture model

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World Journal of Gastroenterology 2005年第38期11卷 5973-5977页

作者： Ersilia Dolfini Luca Elli Leda Roncoroni Barbara costa Maria Pia Colleoni Vito Lorusso Simona Ramponi Paola Braidotti Stefano Ferrero Maria Letizia Falini Maria teresa Bardella Department of Biology and Genetics for Health Sciences Department of Gastroenterology Department of Biotechnology and Bioscience Department of Pharmacology Bracco Imaging SpA Department of Medicine Department of Agrifood Molecular Science

AIM： To evaluate the effects of gliadin on the oxidative environment in the “in vivo-like”model of a three-dimensional cell culture system. METHODS： LoVo cell line （intestinal adenocarcinoma） multicellular spheroids were treated with digested gliadin （with albumin used as a control）. Spheroid volumes, cell viability and morphology, lactate dehydrogenase （LDH） release, content of reduced glutathione （GSH） and activity of GSH-related enzymes were examined. The data were statistically analyzed using the Student＇s t-test （P〈0.05）. was considered statistically significant. RESULTS： Gliadin reduced cell viability （from 20% to 60%） and led to morphological alterations characterized by apoptotic findings and cytoskeletal injuries. LDH activity increased. The content of GSH reduced （-20% vscontrols）, and activity of GSH-related enzymes was significantly inhibited. CONCLUSION： Gliadin treatment induces an imbalance in the antioxidative mechanism of cells cultured by the three-dimensional technique. This alteration may explain cell damage directly caused by gliadin and the subsequentmorphological abnormalities.

关键词： Gliadin Celiac disease Cytotoxicity Multicellular spheroids

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