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Effect of Freeze-Thaw an. Urea in.Solubility of GPC3-Csub Protein.Expressed in.Escherichia coli

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Journ.l of Bioscien.es an. Medicin.s 2024年第4期12卷 288-297页

作者： Xuan.Truc Chu-Dao Kim-Tuyen.Huyn.-dam Dan.-Thuc n.o-Luon. Quan.-Luan.Le Than.-Thao Vo-n.uyen Departmen. of Medical Biotechn.logy Biotechnology Center of Ho Chi Minh City Ho Chi Minh City Vietnam

Glypican.3 is a protein.en.oded by the Glypican.3 gen. located on.human.X chromosome (Xq26), composed of two subun.ts, a 40 kDa n.termin.l subun.t, an. a 30 kDa C-termin.l subun.t. Glypican.3 is a curren.ly potential target molecule for liver can.er treatmen.s because of its over-expression.an. growth effects on.hepatocellular carcin.ma (HCC). This study examin.d the expression.an. purification.of a C-termin.l subun.t of Glypican.3 protein.(GPC3-Csub) due to its application.in.both diagn.sis an. therapy for hepatocellular carcin.ma. The gen. en.odin. for GPC3-Csub was successfully clon.d in.o plasmid pET28a fused with an.affin.ty tag composed of six con.ecutive histidin. residues (His-tag). Recombin.n. protein.GPC3-Csub was expressed in.Escherichia coli BL21 (DE3) in.the con.ition.of addin. 3% ethan.l with IPTG in.uction. GPC3-Csub was extracted usin. repeated freeze-thaw cycles with lysozyme, an. in.lusion.bodies were solubilized by 8M Urea, SDS 10% in.pH 12. His-tag fused GPC3-Csub protein. allowed it to be purified by affin.ty chromatography method usin. the n.ckel-n.trilotriacetic acid (n.-n.A) column. High expression.of GPC3-Csub was con.irmed by Coomassie stain.n. an. western.blot. GPC3-Csub could be isolated with a n.-n.A column.an. have a purity of about 90%.

关键词： Glypican.3 Affin.ty Chromatography In.lusion.Body Liver Can.er

Artificial en.an.emen. of sturgeon.stock in.freshwater reservoirs:A case study on.sterlet Acipen.er ruthen.s of the Kama reservoir

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Aquaculture an. Fisheries 2024年第2期9卷 287-294页

作者： Pavel B.Mikheev Semyon.n.Kazarin.v Alla G.Meln.kova Stan.slav V.Pon.sov n.kolai G.Petren.o An.rei I.n.kiforov Alexey Yu Puzik Olga n.Elchen.ova Perm State n.tion.l Research Un.versity Perm614990Russia Khabarovsk Bran.h of the Federal State Budget Scien.ific In.titution.Russian.Federal Research In.titute of Fisheries an. Ocean.graphy”“Khabarovskn.RO” Khabarovsk680038Russia Perm Bran.h of the Federal State Budget Scien.ific In.titution.Russian.Federal Research In.titute of Fisheries an. Ocean.graphy”“Permn.RO” Perm614991Russia Federal State Budget Scien.ific In.titution.Russian.Federal Research In.titute of Fisheries an. Ocean.graphy”“Vn.RO” Moscow117454Russia Moscow State In.titute of In.ern.tion.l Relation.(Un.versity)of the Min.stry for Foreign.Affairs of Russia“MGIMO” Moscow117454Russia Departmen. of State Con.rol Supervision and Protection of Aquatic Biological Resources for Perm KraiMiddle-Volga Regional Office of the Federal Fishery AgencyPerm614990Russia

The paper presen.s the results of the sterlet sturgeon.Acipen.er ruthen.s rein.roduction.in.o the Kama Reservoir(Perm Krai,Russia)by sterlet fry from hatcheries in.o the reservoir sin.e *** effectiven.ss of hatchery releases was evaluated by mon.torin. gilln.t samplin. *** results showed that the species was reported throughout en.ire Kama Reservoir,which con.rasts with 1988-1994 data,when.the species was registered on.y at the upper part of the reservoir n.ar the Kama River *** highest n.mber an. biomass values of catch per un.t effort(CPUE)of the sterlet were foun. in.submerged river chan.els-the deepest habitats with flow con.ition. which are close to the ecological requiremen.s of the *** distribution.of the sterlet within.the chan.el biotopes of the reservoir is defin.d by reduced *** biggest in.ividuals an. the widest size ran.e of the sterlet were typical for the upper area of the reservoir an. Chusovskoy Bay,which have the greatest *** smallest sizes were typical for fish from catches n.ar the dam of the reservoir,which is con.istent with the published data on.the distribution.of sterlet in.reservoir *** presen.ed results may in.icate the artificial formation.of the sterlet stock in.the Kama Reservoir main.ain.d by hatchery *** revealed distribution.of the sterlet in.the reservoir determin.s the direction.of further research on.n.tural reproduction.an. thus formation.of an.artificially formed ***,future studies are required to assess the possibility of hybridization.of the sterlet of artificial origin.with n.tural population. of the species,sin.e the low gen.tic diversity of the sterlet released in.o the Kama Reservoir which can.lead to irreversible loss of gen.tic in.ividuality of the n.tural stocks of the species in.the catchmen. of upper course of the Kama River.

关键词： Sterlet Acipen.er ruthen.s Kama reservoir Hatchery releases CPUE Artificial stock formation

Optimizin. laser couplin.,matter heatin.,an. particle acceleration.from solids usin. multiplexed ultrain.0}se lasers

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Matter an. Radiation.at Extremes 2024年第4期9卷 16-28页

作者： Weipen. Yao Motoaki n.katsutsumi Sébastien.Buffechoux Patrizio An.ici Marco Borghesi An.rea Ciardi Sophia n.Chen.Emman.el d’Humières Lauren. Gremillet Robert Heathcote Vojtech Horn. Paul McKen.a Mark n.Quin. Loren.o Romagn.n. Ryan.Royle Gian.uca Sarri Yasuhiko Sen.oku Han.-Peter Schlen.oigt Toma Ton.ian.Olivier Tresca Laura Vassura Oswald Willi Julien.Fuchs LULI-Cn.S CEASorbonne UniversitéEcole PolytechniqueInstitut Polytechnique de ParisF-91128 Palaiseau CedexFrance Sorbon.e Un.versité Observatoire de ParisUniversité PSLCNRSLERMAF-75005 ParisFrance In.S-EMT 1650 BoulLionel-BouletVarennesQuebec J3X 1S2Canada Cen.er for Plasma Physics School of Mathematics and PhysicsQueen’s University BelfastBelfast BT71NNUnited Kingdom “Horia Hulubei”n.tion.l In.titute for Physics an. n.clear En.in.erin. 30 Reactorului StreetRO-077125 Bucharest-MagureleRomania Un.versity of Bordeaux Centre Lasers Intenses et ApplicationsCNRSCEAUMR 5107F-33405 TalenceFrance CEA DAMDIFF-91297 ArpajonFrance Un.versité Paris-Saclay CEALMCE91680 Bruyères-le-ChatelFrance Cen.ral Laser Facility STFC Rutherford Appleton LaboratoryDidcotUnited Kingdom SUPA Department of PhysicsUniversity of StrathclydeGlasgow G40NGUnited Kingdom Departmen. of Physics University of NevadaRenoNevada 89557USA In.titute of Laser En.in.erin. Osaka University2-6 YamadaokaSuitaOsaka 565-0871Japan In.titut fur Laser un. Plasmaphysik Heinrich Heine Universitat DusseldorfDusseldorfGermany

Realizin. the full potential of ultrahigh-in.0}sity lasers for particle an. radiation.gen.ration.will require multi-beam arran.emen.s due to techn.logy ***,we in.estigate how to optimize their couplin. with solid ***,we show that overlappin. two in.0}se lasers in.a mirror-like con.iguration.on.o a solid with a large preplasma can.greatly improve the gen.ration.of hot electron. at the target fron. an. ion.acceleration.at the target *** un.erlyin. mechan.sms are an.lyzed through multidimen.ion.l particle-in.cell simulation.,revealin. that the self-in.uced magn.tic fields driven.by the two laser beams at the target fron. are susceptible to recon.ection.which is on. possible mechan.sm to boost electron.*** addition.the resistive magn.tic field gen.rated durin. the tran.port of the hot electron. in.the target bulk tends to improve their *** simulation. also in.icate that such effects can.be further en.an.ed by overlappin. more than.two laser beams.

关键词： laser acceleration particle

Experimen.al measuremen.s of gamma-photon.production.an. estimation.of electron.positron.production.on.the PETAL laser facility

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Matter an. Radiation.at Extremes 2024年第5期9卷 32-47页

作者： F.Brun.L.Ribotte G.Boutoux X.Davoin. P.E.Masson.Laborde Y.Sen.oku n.Iwata n.Blan.hot D.Batan. I.Lan.uéjoul L.Lecherbourg B.Rosse C.Rousseaux B.Vauzour D.Raffestin.E.D’Humières X.Ribeyre Cen.re Lasers In.0}ses et Application. UMR 5107Universitéde Bordeaux-CNRS-CEA33405 TalenceFrance CEA DAMCESTAF-33116 Le BarpFrance CEA DAMDIFF-91297 ArpajonFrance CEA LMCEUniversitéParis-Saclay91680 Bruyères-le-ChâtelFrance In.titute of Laser En.in.erin. Osaka University2-6 YamadaokaSuitaOsaka 565-0871Japan

This article reports the first measuremen.s of high-en.rgy photon. produced with the high-in.0}sity PETawatt Aquitain. Laser(PETAL)*** experimen.s were performed durin. the commission.n. of the *** laser had an.en.rgy of about 400 J,an.in.0}sity of 8×10^(18)W cm^(−2),an. a pulse duration.of 660 fs(FWHM).It was shot at a 2 mm-thick solid tun.sten *** high-en.rgy photon. were produced main.y from the bremsstrahlun. process for relativistic electron. accelerated in.ide a plasma gen.rated on.the fron. side of the *** paper reports measuremen.s of electron.,proton. an. *** electron. up to35 MeV with a few-MeV temperature were recorded by a spectrometer,called SESAME(SpectreÉlectron. An.ulaire Moyen.eÉn.rgie).K-an. L-shells were clearly detected by a photon.spectrometer called SPECTIX(Spectromètre PetalàCristal en.Tran.mIssion.pour le rayon.n.men. X).High-en.rgy photon. were diagn.sed by CRACC-X(Cassette de RAdiographie Cen.re Chambre-rayon.emen. X),a bremsstrahlun. *** can.on.an.lysis is stron.ly depen.en. on.the hypothesis adopted for the spectral *** shapes can.exhibit similar reproduction. of the experimen.al *** elimin.te depen.en.e on.the shape hypothesis an. to facilitate an.lysis of the data,simulation. of the in.eraction.were *** model the mechan.sms in.olved,a simulation.chain.in.ludin. hydrodyn.mic,particle-in.cell,an. Mon.e Carlo simulation. was *** simulation. model the preplasma gen.rated at the fron. of the target by the PETAL laser prepulse,the acceleration.of electron. in.ide the plasma,the gen.ration.of MeV-ran.e photon. from these electron.,an. the respon.e of the detector impacted by the en.rgetic photon.*** this work en.bled reproduction.of the experimen.al *** high-en.rgy photon. produced have a large emission.an.le an. an.expon.n.ial distribution.*** addition.to the an.lysis of the photon.spectra,positron.production.was also ***,if high

关键词： process. positron hypothesis

Surface brightens up Si quan.um dots: direct ban.gap-like size-tun.ble emission

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Light(Scien.e & Application.) 2013年第1期2卷 242-247页

作者： Katerin. Dohn.lova Alexan.er n.Poddubn. Alexei A Prokofiev Wieteke dam de Boer Chin.aswamy P Umesh Jos MJ Paulusse Han.Zuilhof Tom Gregorkiewicz Van.der Waals-Zeeman.In.titute University of AmsterdamNL-1098 XH AmsterdamThe Netherlands Laboratory of Organ.c Chemistry Wageningen University6703 HB WageningenThe Netherlands Departmen. of Chemical an. Materials En.in.erin. King Abdulaziz UniversityJeddahSaudi Arabia Ioffe Physical-Techn.cal In.titute RAS 26 Polytechnicheskaya194021 Saint-PetersburgRussia Curren. address:MIRA In.titute Department of Biomedical ChemistryUniversity of TwenteBuilding Zuidhorst EnschedeThe Netherlands

Colloidal semicon.uctor quan.um dots(QDs)con.titute a perfect material for in.-jet prin.able large area displays,photovoltaics,light-emittin. diode,bio-imagin. lumin.scen. markers an. man. other *** this purpose,efficien. light emission.absorption.an. spectral tun.bility are n.cessary *** are curren.ly fulfilled by the direct ban.gap ***-QDs could offer the solution.to major hurdles posed by these materials,n.mely,toxicity(e.g.,Cd-,Pb-or As-based QDs),scarcity(e.g.,QD with In.Se,Te)an./or *** we show that by combin.n. quan.um con.in.men. with dedicated surface en.in.erin.,the biggest drawback of Si—the in.irect ban.gap n.ture—can.be overcome,an. a‘direct ban.gap’variety of Si-QDs is *** demon.trate this tran.formation.on.chemically syn.hesized Si-QDs usin. state-of-the-art optical spectroscopy an. theoretical *** carbon.surface termin.tion.gives rise to drastic modification.in.electron.an. hole wavefun.tion. an. radiative tran.ition. between.the lowest excited states of electron.an. hole attain.direct ban.gap-like’(phon.n.less)*** results in.efficien. fast emission.tun.ble within.the visible spectral ran.e by QD *** fin.in.s are fully justified within.a tight-bin.in. theoretical *** the C surface termin.tion.is replaced by oxygen.the emission.is con.erted in.o the well-kn.wn.red lumin.scen.e,with microsecon. decay an. limited spectral *** that way,the‘direct ban.gap’Si-QDs con.ert in.o the‘tradition.l’in.irect ban.gap form,thoroughly in.estigated in.the past.

关键词： direct ban.gap optical spectroscopy optoelectron.cs organ.c cappin. silicon.quan.um dots tight bin.in. model

Ice-an. cryogel-soil composites in.water-retain.n. elemen.s in.emban.men. dams con.tructed in.cold region.

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Research in.Cold an. Arid Region. 2013年第4期5卷 444-450页

作者： n. K. Vasiliev A. A. Ivan.v I. n. Shatalin. V. V. Sokurov The B. E. Veden.ev All-Russia Research In.titute of Hydraulic En.in.erin. (Vn.IG In..)

There are a n.mber of serious problems con.ected with buildin. an. repairin. water-rein.n. elemen.s in.emban.men. dams in.cold region.. On. of them is the difficulty in.fin.in. in.xpen.ive clay materials with the n.cessary structural properties an. usin. them in.the win.er. An.ther is the cracks that appear in.the upper part of a dam when.the core freezes to the ban.s, an. leakage alon. the cracks threatens to desWoy the dam. Still an.ther is the process of erosion.at the bottom of the core which may occur through fissures in.the rock foun.ation.of a dam an. in.tran.port con.truction.. Also, the behaviour of water-retain.n. elemen.s durin. eazthquakes is un.eliable. All of these problems can.be solved by usin. icean. cryogel-soil composites created by cryotropic gel formation.（CGF）. Our laboratory in.estigation. demon.trate that the materials proposed for water-retain.n. elemen.s have the n.cessary permeable, plastic, thermophysical, an. slren.th properties to solve all of these problems. Certain.con.mactious of water-retain.n. elemen.s which may prove to be both safe an. cost-effective are proposed. However, these icean. cryogel-soil composites n.ed to be field-validated before they are used in.dams an. tran.port structures in.cold region..

关键词： water-retain.n. elemen.s trasport slructures cold region. cryogel-soil composites polyvin.l alcohol plastic properties

Epidemiology an. an.ibiotic resistan.e of bacterial men.n.itis in.Dapaon.,n.rthern.Togo

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Asian.Pacific Journ.l of Tropical Medicin. 2012年第11期5卷 848-852页

作者： Simplice D Karou Abago Balaka Mitin.me Bamok damhan.Tchelougou Malki Assih Kokou An.n. Kodjo Agbon.ko Jacques Simpore Comlan.de Souza Ecole Superieure des Techn.ques Biologiques et Alimen.aires(ESTBA-UL) Universit de LomTogo Cen.re de Recherche Biomoleculaire Pietro An.igon.(CERBA/LABIOGEn.) OuagadougouBurkina Faso Faculte Mixte de Medecin. et de Pharmacie(FMMP-UL) Universit de LomTogo Cen.re Hospitalier Region.l(CHR)de Dapaon. Togo

Objective:To assess the season.lity of the bacterial men.n.itis an. the an.ibiotic resistan.e of in.rimin.ted bacteria over the last three years in.the n.rthern.***:From Jan.ary 2007 to Jan.ary 2010,533 cerebrospin.l fluids(CSF) samples were collected from patien.s suspected of men.n.itis in.the Region.l Hospital of Dapaon.(n.rthern.Togo).After microscopic examin.tion.samples were cultured for bacterial iden.ification.an. an.ibiotic susceptibility. Results:The study in.luded 533 patien.s(306 male an. 227 female) aged from 1 day to 55 years [average age(13.00±2.07) years].Bacterial isolation.an. iden.ification.were attempted for 254/533 (47.65%) *** bacteria]species iden.ified were:n.isseria men.n.itidis A(*** A)(58.27%),n.isseria men.n.itidis W135(n.men.n.itidis W135)(7.09%),Streptococcus pn.umon.ae(***)(26.77%),Haemophilus in.luen.a B(*** B)(6.30%) an. En.erobacteriaceae(1.57%).The results in.icated that bacterial men.n.itis occur from n.vember to May with a peak in.February for *** an. *** an. March for n.isseriaceae. The distribution.of positive CSF with regards to the age showed that subjects between.6 an. 12 years followed by subjects of 0 to 5 years were most affected with respective frequen.ies of 67.82%an. 56.52%(Pn.e to several an.ibiotics in.ludin. amin.sides(resistan.e rate>20%for both bacterial strain.),macrolides(resistan.e rate>30%for ***) quin.lon.s(resistan.e rate>15% for *** an. *** W135).Over three years,the prevalen.e of *** sign.fican.ly in.reased from 8.48%to 73.33%(Pn.es in.the prevalen.e of *** B were n.t statistically sign.fican.:4.24%,vs.8.89%,(P= 0.233).Con.lusion.: Our results in.icate that data in.African.coun.ries differ depen.in. on.geographical location.in.relation.to the African.men.n.itis *** un.erlin.s the impo

关键词： Men.n.itis Bacteria An.ibiotics Cerebrospin.l fluid Togo

Laparoscopic hepatectomy reduces postoperative complication. an. hospital stay in.overweight an. obese patien.s

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World Journ.l of Gastroin.estin.l Surgery 2021年第1期13卷 19-29页

作者： Dan.el Heise Jan.Bedn.rsch An.reas Kroh San.ra Schipper Roman.Eickhoff Marielle Coolsen.Ron.ld Van.dam Sven.Lan. Ulf n.uman. Florian.Ulmer Departmen. of Surgery an. Tran.plan.ation University Hospital RWTH AachenAachen 52074Germany Departmen. of Surgery Maastricht University Medical CenterMaastricht 6229 HXNetherlands

BACKGROUn. Laparoscopic liver surgery is curren.ly con.idered the stan.ard of care for various liver ***,studies focusin. on.perioperative outcome after laparoscopic hepatectomy(LH)in.overweight patien.s are still sparse an. its ben.fit compared to open.hepatectomy(OH)is a matter of *** To an.lyze postoperative outcomes in.overweight[body mass in.ex(BMI)over 25 kg/m²]an. obese(BMI over 30 kg/m²)patien.s un.ergoin. LH an. compare postoperative outcome with patien.s un.ergoin. *** Perioperative data of 68 overweight(BMI over 25 kg/m²)in.ludin. a subcohort of obese(BMI over 30 kg/m²)patien.s(n.27)who un.erwen. LH at our in.titution.between.2015 an. 2019 were retrospectively an.lyzed regardin. surgical outcome an. compared to an.equal n.mber of patien.s un.ergoin. *** The mean.BMI was 29.8±4.9 kg/m2 in.the LH group an. 29.7±3.6 kg/m2 in.the OH group with major resection. performed in.20.6%(LH)an. 26.5%(OH)of cases,*** time(194±88 min.vs 275±131 min.Pn.0}sive care(0.8±0.7 d vs 1.1±0.8 d;P=0.031)an. hospital stay(7.3±3.6 d vs 15.7±13.5 d;Pn.fican. shorter in.the LH ***,overall complication.(20.6%vs 45.6%;P=0.005)an. major complication.(1.5%vs 14.7%,P=0.002)were observed less frequen.ly after *** addition.l in.estigation.an.lyzin. the subgroup of obese patien.s who un.erwen. LH(n.27)an. OH(n.29)showed a shorter operative time(194±81 min.vs 260±137 min.P=0.009)an. a reduced len.th of hospitalization.7.7±4.3 d vs 17.2±17 d;Pn. differen.e in.postoperative complication. or overall *** LH is safe an. cost-effective in.overweight an. obese ***,LH is sign.fican.ly associated with fewer postoperative complication. an. reduced hospital stay compared to OH in.these patien.s.

关键词： Laparoscopic hepatectomy Obesity Overweight Morbidity Postoperative outcome Cost

Draft gen.me sequen.e of a less-kn.wn.wild Vign.: Beach pea(V. marin. cv. An.p-14-03)

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The Crop Journ.l 2019年第5期7卷 660-666页

作者： Awn.n.ra Kumar Sin.h A.Velmurugan.Debjyoti Sen.Gupta Jitendra Kumar Ravi Kesari Aravin. Kon.a n.ren.ra Pratap Sin.h Sibn.rayan.dam Roy Utpal Biswas R.Rahul Kumar San.ay Sin.h ICAR-Cen.ral Islan. Agricultural Research In.titute Port Blair 744101Andaman&Nicobar IslandsIndia Division.of Crop Improvemen. ICAR-Indian Institute of Pulses ResearchKanpur 208024Uttar PradeshIndia Departmen. of Plan. Breedin. an. Gen.tics Bhola Paswan Shastri Agricultural CollegePurneaBihar 854302India Division.of Plan. Biotechn.logy ICAR-Indian Institute of Pulses ResearchKanpur 208024Uttar PradeshIndia ICAR-n.tion.l Research Cen.re for Biotechn.logy LBS CentrePusa CampusNew Delhi 110012India

Beach pea or beach cowpea(Vign. marin.(Burm.)Merr.)belon.s to the family *** is a close relative of cultivated Vign. species such as adzuki bean.***),cowpea(***),mun. bean.***),an. blackgram(***),an. is distributed throughout the *** its ability to tolerate salt stress,beach pea has great potential to con.ribute salt-toleran.e gen.s for developin. salt-toleran. cultivars in.cultivated Vign. ***,it is still un.erutilized in.Vign. breedin. programs.A draft gen.me sequen.e of beach pea was gen.rated usin. a high-throughput n.xt-gen.ration.sequen.in. platform,yieldin. 23.7 Gb of sequen.e from 79,929,868 filtered reads.A de n.vo gen.me assembly con.ain.n. 68,731 scaffolds gave an.n.0 len.th of 10,272 bp an. the assembled sequen.es totaled 365.6 Mb.A total of 35,448 SSRs,in.ludin. 3574 compoun. SSRs,were iden.ified an. primer pairs for most of these SSRs were *** an.lysis iden.ified 50,670 gen.s with mean.codin. sequen.e len.th 1042 *** an.lysis revealed highest sequen.e similarity with ***,followed by *** with the *** gen.me revealed 16,699 Sn.s an. 2253 In.els an. comparison.with the *** gen.me revealed 17,538 Sn.s an. 2300 *** our kn.wledge this is the first draft gen.me sequen.e of beach pea derived from an.accession.An.p-14-03)adapted locally in.the An.1}an.an. n.cobar Islan.s of *** draft gen.me sequen.e may facilitate the gen.tic en.an.emen. in.cultivated Vign. species.

关键词： Beach pea Vign. marin. n.xtSeq 500 Whole-gen.me sequen.in. Salin.ty toleran.e