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Electron probe microanalysis оf experimentally stimulated osteoarthrosis in dogs

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World Journal of Orthopedics 2017年第9期8卷 681-687页

作者： tatyana Stupina Michael Shchudlo Michael Stepanov Laboratory of Morphology FSBI Russian Ilizarov Scientific Center "Restorative Traumatology and Orthopaedics"

AIM To develop methods of articular cartilage preparation for X-ray-electron probe microanalysis and to study its elements content in experimental *** Twenty dogs aged 2-8 years were divided in research(aged 2 years, induction of osteoarthrosis-IOA) and intact group. Intact group included three subgroups(aged 2, 5 and 8 years). Samples of cartilage after araldite saturation and pouring were partially cut into semithin sections stained with methylene blue and with methylene blue-basic fuchsin. Their smooth surfaces were investigated by X-ray-electron probe microanalysis. Spatial distribution of sulfur, calcium and phosphorus and their concentrations(weight %) were *** X-ray electron probe microanalysis revealed non-uniformsulfur distribution in cartilage of intact animals: Its content increases from superficial zone to deep one, this regularity was preserved in animals with IOA. Differences of IOA with spontaneous chondropathy were revealed. Spontaneous aging was characterized by calcium and phosphorus storage in deep and calcified zones and compensatory increase of sulfated glycosaminoglycans in intermediate and deep cartilage zones as evidenced by the metachromatic reaction and microanalysis data. Unlike spontaneous chondropathy connected with aging in experimentally stimulated osteoarthrosis more intensive storage of calcium but minor phosphorus in intermediate zone were marked. In IOA the calcified cartilage thinning and osteoclastic resorption are apparent with few changes of elements composition; the only difference from control is minority phosphorus *** The obtained results demonstrate specific tricks of X-ray electron probe microanalysis and its possibility in the research of mechanisms of articular cartilage alterations in osteoarthrosis.

关键词： Osteoarthrosis Articular cartilage Dog X-ray electron probe microanalysis

Irrigation erosion of irrigated soils in the foothills of southern Kazakhstan

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Journal of Arid Land 2013年第2期5卷 166-171页

作者： Abdulla S SAPAROV Enadiy K МIRZAKEEV tatyana M SHARYPOVA Galymzhan A SAPAROV Jilili ABUDUWAILI Kazakh Research Institute of Soil Science and Agrochemistry named after Uspanov Almaty 050060 Kazakhstan State Key Laboratory of Desert and Oasis Ecology Xinjiang Institute of Ecology and Geography Chinese Academy of SciencesUrumqi 830011 China

Abstract： This paper presented the results from the study of irrigation erosion of irrigated lands in southern Ka- zakhstan. The main purpose of the work is the conservation of the fertility of irrigated soils, and then the soils of the upper, middle and lower sections of the slopes at the experimental site were studied. Based on field investigations, authors studied the water resistance of aggregates of gray-brown soils and light gray soils before and after irrigation and qualitative indicators of changes in physical and chemical properties of structured soils in irrigation were de- termined by conventional methods of soil research. The results indicated that the changes in the physical properties of soils by using polymeric compounds created a fundamentally new opportunity to control water erosion of soils.

关键词： irrigation erosion furrow irrigation structure-forming polymers deformation of furrows permissible water speed aggregate water stability

Ketogenic diet versus ketoacidosis： what determines the influence of ketone bodies on neurons？

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Neural Regeneration Research 2018年第12期13卷 2060-2063页

作者： Sergei V.Fedorovich Polina P.Voronina tatyana V.Waseem Institute of Biophysics and Cell Engineering Minsk Belarus Department of Pharmacology University of Oxford Oxford UK

Glucose is the main energy substrate for neurons, however, at certain conditions, e.g. in starvation, these cells could also use ketone bodies. This approach is used in clinical conditions as the ketogenic diet. The ketogenic diet is actually a biochemical model of fasting. It includes replacing carbohydrates by fats in daily meal. Synthesis of ketone bodies β-hydroxubutirate, acetoacetate and acetone begins once glycogen stores have depleted in the liver. The ketogenic diet can be used to treat clinical conditions, primarily epilepsy. The mechanism of neuroprotective action of ketogenic diet is not very clear. It is shown that ketone bodies influence neurons at three different levels, namely, metabolic, signaling and epigenetic levels. Ketone bodies are not always neuroprotective. Sometimes they can be toxic for the brain. Ketoacidosis which is a very dangerous complication of diabetes mellitus or alcoholism can be taken as an example. The exact mechanism of how neuroprotective properties of ketone bodies reverse to neurotoxic is yet to be established.

关键词： β-hydroxybutirate epilepsy diabetes mellitus alcoholism metabolism hydroxyl-carboxylic acid receptor epigenetics acidosis

Growth rate of CO_(2) hydrate film on water-oil and water-gaseous CO_(2) interface

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Chinese Journal of Chemical Engineering 2023年第4期56卷 266-272页

作者： tatyana P.Adamova Sergey S.Skiba Andrey Yu.Manakov Sergey Y.Misyura Kutateladze Institute of Thermophysics SB RAS Lavrentiev Ave.1Novosibirsk 630090Russian Federation

It is known that injection of carbon dioxide into the petroleum reservoir(CO_(2) flooding) is one of the effective methods for enhanced oil recovery. CO_(2) flooding may be complicated by formation of CO_(2) hydrate plugs. It makes topical investigation of CO_(2) hydrate formation in the system gaseous CO_(2)-oil-water. In this work, the growth rates of carbon dioxide hydrate films at the water-oil as well as the water-gas interface are studied in the pressure range of 2.30-3.04 MPa and at temperatures between -5.4 and 5.0℃. It is found that the growth rate for the water-oil interface is 3.5 times lower than that for the water-gas interface with carbon dioxide. It is hypothesised that the observed decrease in the growth rate is related to the mechanical resistance of the oil components adsorbed on the interface to the growth of the hydrate film. The growth rate of the film has been shown to depend on the experimental procedure,most likely due to the different initial concentrations of carbon dioxide in the aqueous solutions.

关键词： Gas hydrate Oil Carbon dioxide Growth rate Hydrate film

Geohelminths eggs contamination of sandpits in Vladivostok, Russia

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Asian Pacific Journal of Tropical Medicine 2016年第12期9卷 1190-1192页

作者： Moskvina tatyana Vladimirovna Bartkova Albina Dmitrievna Ermolenko Alexey Vasil'evich School of Natural Sciences Far Eastern Federal University Center of hygiene and epidemiology in Primorskii Region Institute of Biology and Soil Science Far-Eastern Branch of Russian Academy of Sciences

Objective: To detect nematode eggs in sandpits in urban area in Vladivostok. Methods: Totally 60 samples were collected from five districts. Sand samples were analyzed using combined flotation-sedimentation method. In result, 18 sand samples were positive for at one or two parasites species. Results: Overall, only 3 genera of helminths were recovered. Most samples were positive for Toxocara spp. eggs. Toxocara spp. eggs were found in each district. More samples with Toxocara spp. eggs were found in Pervomaiskii district. Ascaris spp. eggs were also appeared in each district, however Ascaris eggs rate of contamination was less than rate of Toxocara spp. contamination. Toxascaris leonina eggs were the less frequent species in sandpits, it was found only in Sovetskii and Pervomaiskii districts. Ascaris eggs rate of contamination was less than rate of Toxocara spp. contamination. Toxascaris leonina eggs were the less frequent species in sandpits, it was found only in two districts. No correlation between districts and helmiths eggs present in soil samples was found(P>0.05). Conclusions: Considering the high sand contamination with parasite infective elements of both human and animal origin, measures to improve environmental and sanitary conditions are indicated, as well as promoting the concept of responsible pet ownership.

关键词： Geohelminths Zoonosis Contamination Parasites

Polychromatic Fourier-PAM fluorometry and hyperspectral analysis of chlorophyll fluorescence from Phaseolus vulgaris leaves: Effects of green light

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Information Processing in Agriculture 2020年第2期7卷 204-211页

作者： Vladimir Lysenko Ya Guo Alexey Kosolapov Elena Usova tatyana Varduny Vladimir Krasnov Southern Federal University Academy of Biology and BiotechnologyRostov-on-DonRussia Russian Information-Analytical and Water Management Research Center Rostov-on-DonRussia School of IoT Engineering Jiangnan UniversityWuxiChina Rostov State Medical University Department of General and Clinical Biochemistry No.1Rostov-on-DonRussia

Maximal and partial quantum yields of photosystem II(Fv/Fm and Fv–/Fp)in Phaseolus vulgaris leaves were determined in response to the turning on saturating and low(undersaturating)actinic light,*** lights(ML)of blue,green,and red colors were applied simultaneously using a novel method of the polychromatic fast Fourier transforming pulse amplitude modulation(FFT PAM)chlorophyll *** of ML were cross-combined with the colors of low and saturating actinic ***/Fm values measured with the green ML were found to be close to that of red light,whereas application of blue ML leads to lower Fv/*** addition,5-nm resolved excitation spectra were measured to evaluate the dependence between red/far red fluorescence ratios(peak height based–F685/F730,and deconvolved peak square based–F685sq/F730sq)and wavelength of the excitation *** demonstrates that chlorophyll fluorescence ratio red/far red may be dependent on the spectral absorbance of *** data obtained confirm the results of the previous studies which explain high photosynthetic activity of the green light in terms of redistribution of absorbed quanta throughout thickness of the leaf,thus suggesting possible advisability of green light application in greenhouse lighting,especially for the plants having thick leaves.

关键词： Multicolor PAM Fourier transforms Chlorophyll fluorescence Green light Red/far red ratio Greenhouse lighting

Strengthening biological security after COVID-19:Using cartoons for engaging life science stakeholders with the Biological and Toxin Weapons Convention(BTWC)

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Journal of Biosafety and Biosecurity 2022年第1期4卷 68-74页

作者： tatyana Novossiolova Simon Whitby Malcolm Dando Lijun Shang The Law Program of the Center for the Study of Democracy Bulgaria Division of Peace Studies University of Bradford BradfordUnited Kingdom School of Human Sciences London Metropolitan UniversityLondonUnited Kingdom Biological Security Research Centre London Metropolitan UniversityLondonUnited Kingdom

The devastating effects of the COVID-19 pandemic have acutely shown the need for maintaining robust international and national systems for biological security and ensuring that life sciences are used only for peaceful *** science stakeholders can play an important role in safeguarding scientific and technological advances in biology and related fields against accidental or deliberate misuse,not least because they are on the frontlines of driving *** this paper,we argue that enhancing awareness and understanding of the risk of deliberate disease is essential for effective biological *** first discuss the issue of‘dual use’in science and technology as it relates to disarmament and non-proliferation of weapons of mass ***,we review how scientist engagement with dual-use risks has been addressed in the context of the Biological and Toxin Weapons Convention(BTWC).Third,we report on the development of an innovative awarenessraising tool,a cartoon series,that can be used for engaging life science stakeholders with BTWC ***,we outline a set of practical considerations for promoting sustainable life science engagement with the BTWC.

关键词： Cartoon Life sciences Biological security Biological and Toxin Weapons Convention Awareness Engagement

GIS tools for correlation of tectonics and seismicity in the Altay-Sayan area,Russia

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Geoscience Frontiers 2010年第1期1卷 133-141页

作者： Raissa M.Lobatskaya tatyana G.Krasnoramenskaya Irkutsk State Technological University Irkutsk 664074Russia Center for Environment and Resources Conservation Krasnoyarsk 660049Russia

Fault-block structures of the Altay-Sayan folded area （ASFA） southeastern Siberia of Russia were used as the basis for creating a 3-D model. The surface structures were projected to depths by previous correlations between long and deep faults, with all layers and deformation factors defined. The mean deformation factor （Ds） is 0.12 unit/km^3 in the upper layer, 0.012 unit/km^3 in the intermediate layer, and 0.007 unit/km^3 in the lower layer of the 3-D ASFA neotectonic model. Ds allows correlation of the three distinguished layers with theological bodies that differ in their potential for accumulating elastic energy. 3-D modeling can be used as a methodological approach to projections in seismic prone areas such as the Krasnoyarsk region, for earthquake-hazard monitoring.

关键词： Fault-block structures 3-D modeling GIS methods Earthquake-hazard Altay-Sayan folded area Russia

3,6-Bis-β -Dicarbonylsubstituted Carbazoles Bearing N-Spacers and Their Eu(III) Complexes as Immunofluorescent Labelling Agents

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