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Open Journal of Preventive Medicine 2015年第12期5卷 456-462页

作者： Masahiro Toda Nobuhiro Nishio tatsuya takeshita Graduate School of Human Life Sciences Notre Dame Seishin University Okayama Japan Department of Public Health Wakayama Medical University Wakayama Japan

We investigated factors contributing to smartphone dependence. To 196 medical university students, we administered a set of self-reporting questionnaires designed to evaluate demographic characteristics, smartphone dependence, chronotype, and depressive state. Smartphone dependence was evaluated using the Wakayama Smartphone-Dependence Scale (WSDS) with 3 subscales: Subscale 1, immersion in Internet communication;Subscale 2, using a smartphone for extended periods of time and neglecting social obligations and other tasks;Subscale 3, using a smartphone while doing something else and neglect of etiquette. Multiple regression analyses revealed that living in a family, eveningness, and presence of depression were associated with Subscale 1, that living in a family and eveningness were also associated with Subscale 2, and that being a man was associated with Subscale 3. These findings suggest that smartphone dependence can be predicted by factors such as gender, mode of residence, chronotype, or depressive state.

关键词： Chronotype Depressive State Smartphone University Students Wakayama Smartphone-Dependence Scale (WSDS)

Relationship of Smartphone Dependence to General Health Status and Personality Traits among University Students

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Open Journal of Preventive Medicine 2016年第10期6卷 215-221页

作者： Masahiro Toda Satoko Ezoe Kanae Mure tatsuya takeshita Graduate School of Human Life Sciences Notre Dame Seishin University Okayama Japan Department of Public Health Wakayama Medical University Wakayama Japan Shimane University Health Service Center Izumo Shimane Japan

We investigated associations between smartphone dependence and general health status or personality traits. To 197 medical university students, we administered a set of self-reporting questionnaires designed to evaluate these parameters. For males, smartphone dependence positively correlated with somatic symptoms, anxiety and insomnia, and emotional instability, and negatively correlated with agreeableness. For females, smartphone dependence positively correlated with somatic symptoms, severe depression, and extraversion, and negatively correlated with social dysfunction. These findings suggest that smartphone dependence may be associated with general health status or personality traits and that there may be a gender difference in these associations.

关键词： Dependence General Health Status Personality Traits Smartphone University Students Wakayama Smartphone-Dependence Scale (WSDS)

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砷对人淋巴细胞DNA氧化损伤的作用

中华预防医学杂志 2002年第1期36卷 12-15页

作者：李达圣 Kanehisa Morimoto tatsuya takeshita 吕玉泉贵州省卫生防疫站地方病防治所贵阳550004 日本大阪大学医学部环境医学教室

目的　探讨砷 (As)引起植物血凝集素 (PHA)刺激和无刺激人外周血淋巴细胞DNA氧化性损伤。方法　用 10 μmol/L砷处理细胞 2h ,经单细胞凝胶电泳 (SCGE ,或彗星试验 ) FPG(甲酰胺基嘧啶 DNA糖基化酶 )消化法检测砷引起的DNA碱基损伤。... 详细信息

目的　探讨砷 (As)引起植物血凝集素 (PHA)刺激和无刺激人外周血淋巴细胞DNA氧化性损伤。方法　用 10 μmol/L砷处理细胞 2h ,经单细胞凝胶电泳 (SCGE ,或彗星试验 ) FPG(甲酰胺基嘧啶 DNA糖基化酶 )消化法检测砷引起的DNA碱基损伤。结果　砷引起的DNA链断裂的修复过程与过氧化氢 (H2 O2 )引起的修复过程类似。FPG消化产生的单链断裂 ,或砷引起的碱基损伤在PHA刺激淋巴细胞较未刺激细胞显著。在PHA刺激的淋巴细胞 ,砷和H2 O2 引起的DNA链断裂 2h分别修复 63 %和 68% ,但在未刺激细胞分别修复大约 3 4 %和 4 3 %。在PHA刺激的淋巴细胞 ,砷和H2 O2引起的碱基损伤 2h分别修复 4 0 %和 4 9% ,但在未刺激细胞分别修复大约 19%和 2 1%。结论　微量砷可引起人类细胞DNA氧化性损伤。损伤的碱基主要是嘌呤或甲酰胺基嘧啶。未分裂 (刺激 )淋巴细胞修复砷与H2 O2

关键词：砷彗星试验 DNA损伤淋巴细胞核苷酶类

氧自由基清除剂对砷所致细胞DNA损伤的保护作用

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中国地方病学杂志 2001年第4期20卷 252-254页

作者：李达圣 Kanehisa Morimoto tatsuya takeshita 吕玉泉贵州省卫生防疫站贵州贵阳550004 大阪大学大学院环境医学教室

目的　探讨砷引起细胞 DNA损伤和细胞毒性的机理。方法　用羟基自由基 (· OH/OH- )清除剂DMSO和过氧化氢 (H2 O2 )清除剂过氧化氢酶 (catalase,CAT)与不同浓度的砷共同处理人类早幼粒细胞系 HL6 0细胞和人淋巴细胞 ,用单细胞凝胶电泳 (... 详细信息

目的　探讨砷引起细胞 DNA损伤和细胞毒性的机理。方法　用羟基自由基 (· OH/OH- )清除剂DMSO和过氧化氢 (H2 O2 )清除剂过氧化氢酶 (catalase,CAT)与不同浓度的砷共同处理人类早幼粒细胞系 HL6 0细胞和人淋巴细胞 ,用单细胞凝胶电泳 (SCGE)检测细胞 DNA损伤。结果　 2 .4和 4.8μmol的砷分别引起了HL6 0和人淋巴细胞显著的 DNA损伤。DMSO和 CAT完全消除了低剂量砷引起的 DNA损伤 ,减少了 70 %以上的高剂量砷引起的 DNA损伤。结论　微量砷可引起人类细胞不同程度的 DNA损伤 ,DMSO和 CAT可保护或减轻细胞的这些损伤 ,表明砷通过活性氧自由基 ,主要是 H2 O2 和 O2 - ,引起人类细胞

关键词：氧自由基清除剂过氧化氢酶砷中毒人类细胞 DNA损伤

Effects of Meat Intake Frequency and Polymorphic Cytochrome P450 2A6 Activity on Individual Colorectal Tumour Risk in a Japanese Cohort

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Journal of Cancer Therapy 2017年第7期8卷 645-652页

作者： Hiroshi Yamazaki Masaki Fujieda Makiko Shimizu Akiko Shiotani Mie Shimabukuro Kanae Mure tatsuya takeshita Hideki Ishikawa Laboratory of Drug Metabolism and Pharmacokinetics Showa Pharmaceutical University Machida Tokyo Japan Faculty of Pharmaceutical Sciences Toho University Funabashi Japan Otemae College of Nutrition and Confectionery Chuo-ku Osaka Japan Department of Public Health Wakayama Medical University Wakayama Japan Department of Molecular-Targeting Cancer Prevention Kyoto Prefectural University Sakyo-ku Kyoto Japan

The relationships among meat consumption, smoking habits, and phenotypic cytochrome P450 2A6 variation with respect to colorectal cancer risk remain unclear. In this study, the relationships among colorectal tumour risk, meat consumption by questionnaire, and phenotypic P450 2A6 activity by genotyping in a case-control study (299 cases and 170 controls) were analyzed for never-smokers and ever-smokers. In never-smokers consuming ≥1 serving per day of total meat, a significant odds ratio of 4.42 (95% confidence interval, 1.29 - 15.2), adjusted by logistic regression for age and gender, was observed, compared with the group consuming ≤2 servings per week. Furthermore, in Japanese never-smokers, the susceptibility to colorectal tumours was dependent on the frequency of meat intake (trend test p = 0.011). In never-smokers who were P450 2A6 poor metabolizers and had a high frequency of meat intake, the apparent odds ratio was 3.57 (95% confidence interval, 0.30 - 42.2) compared with the P450 2A6 normal group with a low meat intake frequency. These results suggested that colorectal tumour risk was inversely associated with the phenotypic P450 2A6 activities in Japanese never-smokers with a high meat intake.

关键词： Meat Consumption Colon Cancer Risk Susceptibility Non-Smoking Subjects CYP2A6

Effect of Genetic Polymorphism of CYP2A6 on Individual Susceptibility to Colorectal Tumors in Japanese Smokers

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Journal of Cancer Therapy 2012年第4期3卷 207-215页

作者： Asami Muroi Kazuma Kiyotani Masaki Fujieda Hideki Ishikawa tatsuya takeshita Shunsuke Iwano Hiroshi Yamazaki Tetsuya Kamataki Department Molecular-targeting Cancer Prevention Kyoto Prefectural University Sakyo-ku Kyoto Japan Department of Public Health Wakayama Medical University Wakayama Japan Laboratory of Drug Metabolism Graduate School of Pharmaceutical Sciences Hokkaido University Sapporo Japan

Tobacco smoking is a risk factor for colorectal cancer and adenomas. To clarify the effect of genetic factors on the risk for tobacco-related colorectal tumors in a Japanese population, we performed a case-control study on 300 patients with two or more tumors and 181 healthy controls;all were genotyped for CYP2A6*4, CYP2A6*7 and CYP2A6*9. Cigarette smoking increased colorectal tumor risk (trend-test P ). Current smokers plus ex-smokers (ever-smokers) with the CYP2A6*4/*4 genotype (whole gene deletion) showed the lowest risk among smokers [odds ratio (OR), 0.17;95% confidence interval (CI), 0.05 - 0.62 compared to ever-smokers with the wild-type CYP2A6*1/*1]. When the participants were classified into four phenotype groups based on estimated CYP2A6 activity [i.e., normal (*1/*1), intermediate (heterozygotes for the *1 and a variant allele), slow (heterozygotes and homozygotes for variant alleles except for *4/*4) and poor (*4/*4)], the ORs (95% CIs) in ever-smokers of the normal, intermediate, slow and poor groups were 6.75 (2.73 - 16.76), 4.59 (2.10 - 10.06), 3.89 (1.69 - 8.95) and 1.17 (0.31 - 4.40), respectively, compared with never-smokers with normal CYP2A6 activity. The susceptibility to colorectal tumors was dependent on the predicted phenotype among ever-smokers (trend-test P = 0.015), but not among never-smokers (trend-test P = 0.47). Stratifying the subjects with respect to cumulative tobacco exposure and estimated CYP2A6 activity, we found the highest risk of colorectal tumors in subjects with higher CYP2A6 activity and higher cumulative tobacco exposure (trend-test P = 0.000023);the lowest risk was found in subjects with the lowest estimated CYP2A6 activity independent of tobacco exposure (trend-test P = 1.00). These results suggest that the gene-environment interaction (i.e. , the CYP2A6-smoking interaction) strongly affects the individual susceptibility to tobacco-related colorectal tumors.

关键词： P4502A6 Tobacco Smoking Colorectal Cancer Colorectal Adenomas Case-Control Study

Genetic Polymorphisms in ADH1B and ALDH2 Are Associated with Colorectal Tumors in Japan: A Case-Control Study

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Journal of Cancer Therapy 2015年第12期6卷 1054-1062页

作者： Akiko Shiotani Hideki Ishikawa Michihiro Mutoh tatsuya takeshita Tomiyo Nakamura Kanehisa Morimoto Toshiyuki Sakai Keiji Wakabayashi Nariaki Matsuura Department of Molecular Pathology Osaka University Graduate School of Medicine & Health Science Osaka Japan Department of Molecular-Targeting Cancer Prevention Kyoto Prefectural University of Medicine Kyoto Japan Epidemiology and Prevention Division Research Center for Cancer Prevention and Screening National Cancer Center Tokyo Japan Department of Public Health Wakayama Medical University School of Medicine Wakayama Japan Department of Food Nutrition Faculty of Agriculture Ryukoku University Shiga Japan Department of Hygiene and Preventive Medicine Osaka University Graduate School of Medicine Osaka Japan Graduate Division of Nutritional and Environmental Sciences University of Shizuoka Shizuoka Japan

Background: Unlike with esophageal cancer, acetaldehyde levels and genetic polymorphisms in alcohol dehydrogenase have not yet been shown to be contributing factors for colorectal cancer (CRC). This study aimed to clarify the mechanism of CRC development related to alcohol consumption and to the presence of genetic polymorphisms in the alcohol dehydrogenase, ADH1B and aldehyde dehydrogenase, ALDH2. Methods: This was a case-control study (221 cases and 179 controls) in patients with adenomas and intramucosal tumors who underwent endoscopic removal of all tumors. The amount of alcohol consumption was determined using a self-recorded questionnaire, and the tumor information was obtained from colonoscopy results. Blood samples were taken to analyze the following polymorphisms: ALDH2 Glu504Lys and ADH1B His48Arg. Results: The polymorphisms in ADH1B and ALDH2 had little influence on the development of colorectal adenoma or intramucosal cancer. Patients with ALDH2 (Glu/Glu) were more tolerant of alcohol than those with ALDH2 (Glu/Lys and Lys/Lys). Next, we examined certain combinations of the ADH1B genotypes. In the ALDH2 (Glu/Glu) group, an increased risk (OR = 3.4;95% CI 1.4 - 8.4;P = 0.009) was observed among moderate/heavy drinkers with ADH1B (His/His). In the ALDH2 (Glu/Lys and Lys/Lys) group, an increased risk (OR = 4.2;95% CI 1.1 - 16.7;P = 0.041) was found among moderate/heavy drinkers with ADH1B (Arg/His and Arg/Arg). Conclusions: ADH1B and ALDH2 activity may be involved in the development of CRC.

关键词： Genetic Polymorphisms Colorectal Tumor Alcohol Intake Alcohol Dehydrogenase-1B Aldehyde Dehydrogenase-2

Are <i>Umezu</i>polyphenols in the Japanese plum (<i>Prunus mume</i>) protective against mild hypertension and oxidation? Evidence from a double-blind randomized placebo-controlled trial

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Open Journal of Preventive Medicine 2013年第9期3卷 561-569页

作者： Shigeki Takemura Kouichi Yoshimasu Kanae Mure Jin Fukumoto Nobuhiro Nishio Naomi Kitano Kunihiro Kishida Fumiko Yano Takahiko Mitani tatsuya takeshita Kazuhisa Miyashita Department of Hygiene School of Medicine Wakayama Medical University Wakayama Japan Department of Public Health School of Medicine Wakayama Medical University Wakayama Japan Faculty of Biology-Oriented Science and Technology Kinki University Wakayama Japan Faculty of Biology-Oriented Science and Technology Kinki University Wakayama Japan Wakayama Industry Promotion Foundation Wakayama Japan

Medications or lifestyle changes to prevent or improve hypertension often press considerable efforts on patients suffering from mild hypertension. Beverages including Umezu polyphenols (UP), polyphenols in Japanese plums, may help them to control their blood pressure. Healthy medical students, workers, and community dwellers who had some concerns about their blood pressure were randomized in a doubleblind manner into UP ingesting and placebo groups. The first two samples (medical students and workers) were placed in a preliminary study, and based on the results from that study, and the dose of UP for the community dwellers was determined. All three groups were followed for 5 weeks, and blood pressure, as well as biochemical markers related to hypertension and atherosclerosis and self-perceived quality-of-life outcomes, was monitored during that period. Group × time interactions on blood pressure changes were examined. For the community dwellers, blood pressure changes did not significantly differ between the UP ingesting and placebo groups. And although studies of students and workers showed subtle differences in blood pressure among the UP and placebo groups, a dose-dependent effect of UP on decreasing blood pressure could not be confirmed. On the other hand, anti-oxidative effects of UP were confirmed especially among male drinkers who were community dwellers. For the community dwellers, self-perceived physical health significantly improved in those who ingested UP. In conclusion, UP might prevent oxidation. A longer monitoring period as well as a higher dose of UP might enable us to confirm effects of UP against hypertension.

关键词： Hypertension Prevention Antioxidants Japanese Plum Polyphenol

砷与5-氮胞苷对人淋巴细胞DNA损伤的联合作用

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地方病通报 2002年第1期17卷 1-4页

作者：李达圣 MORIMOTO Kanehisa takeshita tatsuya 吕玉泉贵州省卫生防疫站贵州贵阳550004 大阪大学医学部环境医学教研室

为探讨砷和 5 -氮胞苷对人淋巴细胞 DNA损伤及修复的联合作用 ,应用单细胞凝胶电泳 (SCGE)技术比较研究了 5 -氮胞苷与砷同时和前后作用于人类淋巴细胞产生的联合毒性 ,结果显示 10 μmol/ L 5 -氮胞苷和 10 μmol/ L砷单独处理人淋巴细... 详细信息

为探讨砷和 5 -氮胞苷对人淋巴细胞 DNA损伤及修复的联合作用 ,应用单细胞凝胶电泳 (SCGE)技术比较研究了 5 -氮胞苷与砷同时和前后作用于人类淋巴细胞产生的联合毒性 ,结果显示 10 μmol/ L 5 -氮胞苷和 10 μmol/ L砷单独处理人淋巴细胞 2 h引起明显的 DNA泳动 (彗星尾 ) ,但两试剂引起的 DNA泳动 (彗星尾 )间无显著差异 ,5 -氮胞苷前处理与砷后处理 2 h引起的彗星尾与其单独处理组比较非常显著 ,砷前处理与 5 -氮胞苷后处理引起的彗星尾与其单独处理组比较无显著性差异 ,但较对照组差异显著。 10 μmol/ L 5 -氮胞苷和 10 μmol/ L砷分别单独处理 2 h引起了人淋巴细胞显著的 DNA损伤 (链断裂 ) ,5 -氮胞苷与砷在对淋巴细胞 DNA的损伤上表现为单纯相加作用。 5 -氮胞苷前处理显著增加了细胞对砷的基因毒性的敏感性 ,或砷后处理显著增加了 5 -氮胞苷引起的 DNA损伤 ,5 -氮胞苷后处理 2 h显著抑制了细胞对砷所致

关键词：砷 5-氮胞苷人淋巴细胞 DNA损伤联合作用单细胞凝胶电泳(SCGE)

应用单细胞凝胶电泳比较研究砷对人类细胞DNA的损伤

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中国地方病学杂志 2001年第3期20卷 212-216页

作者：李达圣 Kanehisa Morimoto tatsuya takeshita 吕玉泉贵州省卫生防疫站贵阳550004 大阪大学大学院环境医学教室

目的　探讨不同细胞对砷的基因毒性反应及其机理 ;探讨快速、敏感及经济的细胞基因毒性检测方法。方法　应用单细胞凝胶电泳 (Single cell gel electrophoresis,SCGE)或彗星试验 (com et assay)比较研究。结果　细胞 DNA损伤与砷处理量... 详细信息

目的　探讨不同细胞对砷的基因毒性反应及其机理 ;探讨快速、敏感及经济的细胞基因毒性检测方法。方法　应用单细胞凝胶电泳 (Single cell gel electrophoresis,SCGE)或彗星试验 (com et assay)比较研究。结果　细胞 DNA损伤与砷处理量间呈显著的剂量反应关系和时效关系。受试细胞对砷的基因毒性反应的敏感性强弱顺序为 :PMA- HL 6 0 >DMSO- HL 6 0 >HL 6 0 >淋巴细胞。人工计数彗星尾分级频数与计算机自动测量彗星尾 DNA泳动量的结果完全一致 ,用非参数 Mann- Whitney多变量检验分析彗星尾分级频数的显著性与参数Tukey多变量检验分析量彗星尾 DNA泳动量的显著性完全相同 ,而且不同浓度的砷与 5级彗星尾频数的相关性比其与阳性彗星尾总数间的更为显著。结论　微量砷即可引起细胞不同程度的 DNA损伤 ,不同细胞亚群对砷的基因毒性反应不同。 SCGE是一个简便、快速、敏感及经济的细胞基因毒性检测方法 ,可应用于各级水平的环境与人类疾病 (砷污染 )

关键词：单细胞凝胶电泳 SCGE 砷人类细胞 DNA损伤