检索结果-南通市图书馆

Significance and utility of MR cisternography for diagnosis and treatment of trigeminal neuralgia

Journal of Biomedical Science and Engineering 2013年第3期6卷 365-371页

作者： tatsurou tanaka Shunji Shiiba Eiji Sakamoto Masafumi Oda Shinji Kito Kentaro Ono Nao Wakasugi-Sato Shinobu Matsumoto-Takeda Yuji Seta Yoshiki Imamura Seiji Watanabe Kiyotoshi Inenaga Yasuhiro Morimoto Center for Oral Biological Research Kyushu Dental University Kitakyushu Japan Department of Control of Physical Functions Faculty of Dentistry Kyushu University Fukuoka Japan Department of Oral Diagnostic Science Nihon University School of Dentistry Tokyo Japan Division of Dental Anesthesiology Kyushu Dental University Kitakyushu Japan Division of Diagnostic Radiology Kyushu Dental University Kitakyushu Japan Division of Oral Histology and Neurobiology Kyushu Dental University Kitakyushu Japan Division of Physiology Kyushu Dental University Kitakyushu Japan

This review article provides doctors and dentists with an introduction to the clinical applications and sig- nificance of magnetic resonance cisternography (MRC) for the evaluation of neurovascular compression of the trigeminal nerve in patients with trigeminal neu- ralgia (TN). Specifically, the method and characteris- tics of MRC are first explained using the relevant MR sequences. Next, clinical applications for the diagnosis of TN are discussed. Finally, the significance and util- ity of MRC for effective treatment of patients with TN, such as carbamazepine administration and/or local anesthetic blocks, are presented.

关键词： Trigeminal Neuralgia Diagnosis MR Cisternography Carbamazepine Local Anesthetic Block

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Purification of enzymatically inactive peptidylarginine deiminase type 6 from mouse ovary that reveals hexameric structure different from other dimeric isoforms

引用

Advances in Bioscience and Biotechnology 2011年第4期2卷 304-310页

作者： Hirofumi Taki Tomoharu Gomi Bryan Knuckley Paul R. Thompson Oliver Vugrek Kazuya Hirata tatsurou Miyahara Kouichiro Shinoda Hiroyuki Hounoki Eiji Sugiyama Isao Usui Masaharu Urakaze Kazuyuki Tobe Tetsuya Ishimoto Ran Inoue Ayumi tanaka Hiroki Mano Hirofumi Ogawa Hisashi Mori 不详

The murine peptidylarginine deiminase (PAD) has five isoforms encoded by different genes and participates in a variety of cellular functions through the citrullination of target proteins. The crystal structure of human PAD4 with a dimeric form was previously solved because of the enzyme’s relevance to rheumatoid arthritis. PAD6, abundant in mouse oocytes and eggs, is believed to take part in early events of embryogenesis, but its biochemical properties are little understood. Here we have purified and characterized a recombinant PAD6. A PAD6 cDNA was cloned from mouse ovary RNA and expressed in Escherichia coli through pET29 and pGEX vectors. When benzoyl-L-arginine ethyl ester was used as a substrate, no appreciable activity was detected with a cell homogenate under conditions where a human PAD4 cDNA caused significant activity. Both proteins were affinity-purified to near homogeneity. The circular dichroism spectra of PAD6 and human PAD4 were similar in the far ultraviolet region. On molecular sieving, PAD6 was eluted faster than human PAD4. The cross-linking of PAD6 with dimethyl suberimidate clearly showed six bands on an sodium dodecyl sulfate-polyacrylamide gel. These results indicate that PAD6 can constitute a hexameric structure. The purified PAD6 still showed no enzymatic activity. This unique structure and loss in enzymatic activity is strongly suggested to favor the formation of egg cytoplasmic sheets as the architectural protein.

关键词： Peptidylarginine deiminase Isoform Dimer Hexamer Mouse Oocytes Cytoplasmic sheets.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：