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Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines

World Journal of Gastroenterology 2012年第31期18卷 4162-4168页

作者： Raimundo Fernandes de Araújo Júnior Luiz Alberto Lira Soares Cínthia Raquel da Costa Porto Ranniere Gurgel Furtado de Aquino Hugo Gon alo Guedes Pedro Ros Petrovick tatiane pereira de souza Aurigena Antunes de Araújo Gerlane Coelho Bernardo Guerra department of Morphology/Postgraduate Program in Morphological Sciences Federal University of Rio Grande do NorteNatal 59072-970Rio Grande do NorteBrazil Postgraduate Program in Pharmaceutical Sciences Federal University of PernambucoRecife 50670-901PernambucoBrazil Postgraduate Program in Pharmaceutical Sciences Federal University of Rio Grande do NorteNatal 59012-570Rio Grande do NorteBrazil department of Production and control of drugs Federal University of Rio Grande do SulPorto Alegre 90610-000Rio Grande do SulBrazil department of Food and Drug Administration Federal University of AmazonasManaus 69077-000AmazonasBrazil department of Biophysics and Pharmacology Federal University of Rio Grande do NorteNatal 59072-970Rio Grande do NorteBrazil

AIM：To investigate the cytotoxic effects of spray-dried extracts of Phyllanthus niruri in combination with cis- platin on two cancer cell lines. METHODS： Colorectal carcinoma （HT29） and human hepatocellular carcinoma （HepG2） cells were treated with spray-dried extracts of Phyllanthus niruri （SdePN） either alone or in combination with cisplatin at differ- ent concentrations （0.5 mg/mL and 1 mg/mL） for 4 h and 24 h. To verify and quantify cancer cells treated with these products as well as identify the cell cycle stage and cell viability, we stained the cells with prop- idium iodide and assessed them by flow cytometry. The percentage of cells in different cell cycle phases was quantified and data were expressed as histo- grams. Significant differences between groups were determined using analysis of variance and Bonferroni＇s test, as indicated. A value of P 〈 0.05 was considered to be statistically significant. RESULTS： SdePN had significantly different cyto- toxic effects on HT29 （2.81 4- 0.11 vs 3.51 4- 1.13, P 〉 0.05） and HepG2 （5.07± 0.3 vs 15.9 ± 1.04, P 〈 0.001） cells when compared to control cells for 4 h. SdePN also had significantly different cytotoxic effects on HT29 （1.91 ± 0.57 vs 4.53± 1.22, P 〉 0.05） and HepG2 （14.56 ± 1.6 vs 35.67 ± 3.94, P 〈 0.001） cells when compared to control cells for 24 h. Both cell lines were killed by cisplatin in a dose-dependent manner compared to control cells （HepG2 cells for 4 h： 10.78 ± 1.58 vs 53.89 ± 1.53, P 〈 0.001; 24 h： 8.9 ± 1.43 vs 62.78 ± 1.87, P 〈 0.001 and HT29 cells for 4 h： 9.52 ±0.913 vs 49.86 ± 2.89, P 〈 0.001; 24 h： 11.78 ± 1.05 vs 53.34 ± 2.65, P 〈 0.001）. In HT29 cells, pretreat- ment with SdePN and subsequent treatment with cis-platin resulted in a greater number of cells being killed （12.78 ± 1.01 vs 93.76 ± 1.6, P 〈 0.001）. HepG2 cells showed significant cell killing with treatment with SdePN when combined with cisplatin （12.87 ± 2.78 vs 78.8 ± 3.02, P 〈 0.001）. CONCLUSION： SdePN is selectively toxic ag

关键词： Cisplatin Colorectal cancer Liver cancer Phyllanthus n/ruri Cytotoxic effect

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The effect of abiotic stresses on plant C:N:P homeostasis and their mitigation by silicon

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The Crop Journal 2024年第2期12卷 340-353页

作者： Milton Garcia Costa Renato de Mello Prado Luiz Fabiano Palaretti Jonas pereira de souza Júnior department of Agricultural Production Science School of Agricultural and Veterinarian SciencesSão Paulo State UniversityJaboticabalBrazil

In crop plants, various environmental stresses affect the balance of carbon, nitrogen, and phosphorus(C:N:P), leading to biochemical and physiological alterations and reductions in yield. Silicon(Si) is a beneficial element that alleviates plant stress. Most studies involving silicon have focused on physiological responses, such as improvements in photosynthetic processes, water use efficiency, and antioxidant defense systems. But recent research suggests that stressed plants facing either limited or excessive resources(water, light, nutrients, and toxic elements), strategically employ Si to maintain C:N:P homeostasis, thereby minimizing biomass losses. Understanding the role of Si in mitigating the impact of abiotic stresses on plants by regulating C:N:P homeostasis holds great potential for advancing sustainable agricultural practices in crop production. This review presents recent advances in characterizing the influence of environmental stresses on C:N:P homeostasis, as well as the role of Si in preserving C:N:P equilibrium and attenuating biological damage associated with abiotic stress. It underscores the beneficial effects of Si in sustaining C:N:P homeostasis and increasing yield via improved nutritional efficiency and stress mitigation.

关键词： Nutritional stoichiometry Elemental stoichiometry Beneficial element Environmental stresses Nutritional efficiency Carbon use efficiency Agricultural sustainability

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Starch Orodispersible Film Loaded with Melatonin for Human Supplementation

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Journal of Renewable Materials 2024年第7期12卷 1343-1354页

作者： Fabio Tamanini tatiane Zucchini de souza Creusa Sayuri Tahara Amaral Antônio JoséFelix Carvalho Eliane Trovatti department of Health and Biological Sciences University of Araraquara(UNIARA)São Paulo14801-340Brazil department of Materials Engineering São Carlos School of EngineeringUniversity of São PauloSão Paulo13563-120Brazil

An innovative pharmaceutical form for administering melatonin,based on starch orodispersibleﬁlm(ODF),was designed and *** composition of the ODF included starch as the polymer matrix,the active drug melatonin,and a ***,a natural hormone produced by the pineal gland in the brain,can be absorbed by passive diffusion across the mucous membrane,resulting in improved bioavailability when compared to conventional oral *** study shows a simple and efﬁcient method for preparing melatonin-loaded orodispersibleﬁlms with a physically stable and commercially viable matrix,suitable for use in the pharmaceutical ***ﬁlms were prepared by treating the starch with microwave irradiation,followed by plasticization with glycerol,melatonin loading and drying by solvent *** tests showed theﬁlms’robustness,with a modulus of approximately 97 MPa,indicating good handling *** uptake analysis showed fast water absorption,reaching about 150%within 2 min,indicating its fast oral disintegration *** tests in artiﬁcial saliva and dissolution studies indicated the release of the drug in min-utes,indicating the potential properties of theﬁlm for oral drug *** the best of our knowledge,there is no melatonin ODF based on starch described in the literature.

关键词： Starch orodispersibleﬁlm melatonin drug delivery

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Antimicrobial Activity of a Formulation of <i>Libidibia ferrea</i>L. against Microorganisms of the dental Biofilm

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Advances in Microbiology 2020年第9期10卷 434-442页

作者： Raquel de Oliveira Marreiro Maria Fulgência Costa Lima Bandeira Carina Toda Fábio Correia Sampaio tatiane pereira de souza Gisely Naura Venâncio Wanderleia Monteiro de souza Nikeila Chacon de Oliveira Conde School of dentistry Federal University of Amazonas Manaus AM Brazil Federal University of Paraíba Health Sciences Center Department of Clinical and Social Dentistry Jo&atilde o Pessoa PB Brazil Faculty of Pharmaceutical Sciences Federal University of Amazonas Manaus AM Brazil Manaus Municipal Health department SEMSA Manaus AM Brazil

This study aimed to evaluate the antimicrobial activity of the extract of Libidibia ferrea L. (228.022-INPA) and a mouthwash formulation against microorganisms of the dental biofilm. The extract of Libidibia ferrea L. was prepared and formulated with an alcohol-free mouthwash. The Minimum Inhibitory Concentration—MIC was determined by microdilution and cell viability was evaluated through fluorescence. The MICs for the extract were 4.375 μg/mL (Streptococcus mutans), 3.750 μg/mL (Streptococcus oralis), 4.375 μg/mL (Lactobacillus casei). In contrast, for the mouthwash, the MICs were 6.000 μg/mL (bacteriostatic against Streptococcus mutans and bactericidal against Streptococcus oralis). In 1.250 μg/mL of the extract, the number of viable cells measured by fluorescence tests was 16.47% and 14.12% for Streptococcus mutans and Lactobacillus casei, respectively. For the mouthwash (1.000 μg/mL), 20.60% of viable cells for Streptococcus mutans, and 9.60% for Lactobacillus casei were observed. It can be concluded that the extract and its mouthwash containig Libidibia ferrea L. showed antibacterial activity against microorganisms from the dental biofilm.

关键词： Mouthwash Juca Natural Products

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Antimicrobial Activity of a Formulation of Libidibia ferrea L. against Microorganisms of the dental Biofilm

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Advances in Microbiology（微生物学） 2020年第9期

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Study of interaction between metal ions and quercetin

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Food Science and Human Wellness 2018年第3期7卷 215-219页

作者： Taiane souza de Castilho tatiane Brescovites Matias Keller Paulo Nicolini Jaqueline Nicolini Instituto Federal do Parana-IFPR Lacoppi-Laboratorio de Corantes e Processos PiroliticosDepartamento de QuimicaPalmasPRBrazil

A displacement test based on the interaction between the flavonoid quercetin and an excess of metal chloride allows the determination of the binding constant for the reaction between quercetin and Ca2+,Mg^2+and Ni^2+.The values obtained were 2.20±1.77×10^3 for Ca^2+,1.37±0.59×10^3 for Mg^2+and 7.03±1.04×10^4 for Ni^2+,and all interactions showed type 1:1 stoichiometry,as determined by titration and by the method of continuous variations(Job’s method).The complexion effect was observed qualitatively through a colorimetric change in the medium(yellow→neon yellow)and spectroscopically through a bathochromic shift in the absorption band of quercetin in the presence of *** investigation serves as a tool for the development and testing of materials capable of capturing toxic metal ions or favoring the absorption of beneficial ions(in relation to the human metabolism)through the construction of efficient bioorganic *** results reported herein allow understanding of this detection system,indicating the following ascending order of the binding constants(Mg^2+

关键词： Binding constants Quercetin Antioxidant properties

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Diet and microbiota in inflammatory bowel disease: The gut in disharmony

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World Journal of Gastroenterology 2017年第12期23卷 2124-2140页

作者： Davy CM Rapozo Claudio Bernardazzi Heitor Siffert pereira de souza Coordenacao de Pesquisa Instituto Nacional de Cancer Servico de Gastroenterologia e Laboratório Multidisciplinar de Pesquisa Departamento de Clínica MédicaHospital Universitário Clementino Fraga FilhoUniversidade Federal do Rio de Janeiro D’Or Institute for Research and Education Rua Diniz Cordeiro 30BotafogoRio de JaneiroRJ 22281-100Brazil

Bacterial colonization of the gut shapes both the local and the systemic immune response and is implicated in the modulation of immunity in both healthy and disease states. Recently, quantitative and qualitative changes in the composition of the gut microbiota have been detected in Crohn's disease and ulcerative colitis, reinforcing the hypothesis of dysbiosis as a relevant mechanism underlying inflammatory bowel disease(IBD) pathogenesis. Humans and microbes have coexisted and co-evolved for a long time in a mutually beneficial symbiotic association essential for maintaining homeostasis. However, the microbiome is dynamic, changing with age and in response to environmental modifications. Among such environmental factors, food and alimentary habits, progressively altered in modern societies, appear to be critical modulators of the microbiota, contributing to or co-participating in dysbiosis. In addition, food constituents such as micronutrients are important regulators of mucosal immunity, with direct or indirect effects on the gut microbiota. Moreover, food constituents have recently been shown to modulate epigenetic mechanisms, which can result in increased risk for the development and progression of IBD. Therefore, it is likely that a better understanding of the role of different food components in intestinal homeostasis and the resident microbiota will be essential for unravelling the complex molecular basis of the epigenetic, genetic and environment interactions underlying IBD pathogenesis as well as for offering dietary interventions with minimal side effects.

关键词： Diet Microbiota Epigenetics Crohn’s disease Ulcerative colitis

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Damage-associated molecular patterns in inflammatory bowel disease:From biomarkers to therapeutic targets

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World Journal of Gastroenterology 2018年第41期24卷 4622-4634页

作者： Hayandra Ferreira Nanini Claudio Bernardazzi Fernando Castro Heitor Siffert pereira de souza Servico de Gastroenterologia e Laboratorio Multidisciplinar de Pesquisa Departamento de Clinica MedicaHospital Universitario Clementino Fraga FilhoUniversidade Federal do Rio de JaneiroRio de JaneiroRJ 21941-913Brazil D’Or Institute for Research and Education(IDOR) Rua Diniz Cordeiro 30BotafogoRio de JaneiroRJ 22281-100Brazil

The chronic inflammatory process underlying inflammatory bowel disease (IBD), comprising Crohn's disease and ulcerative colitis, derives from the interplay of several components in a genetically susceptible host. These components include environmental elements and gut microbiota a dysbiosis. For decades, immune abnormalities have been investigated as critically important in IBD pathogenesis, and attempts to develop effective therapies have predominantly targeted the immune system. Nevertheless, immune events represent only one of the constituents contributing to IBD pathogenesis within the context of the complex cellular and molecular network underlying chronic intestinal inflammation. These factors need to be appreciated within the milieu of nonimmune components. Damage-associated molecular patterns (DAMPs), which are essentially endogenous stress proteins expressed or released as a result of cell or tissue damage, have been shown to act as direct proinflammatory mediators. Excessive or persistent signalling mediated by such molecules can underlie several chronic inflammatory disorders, including IBD. The release of endogenous DAMPs amplifies the inflammatory response driven by immune and non-immune cells and promotes epigenetic reprogramming in *** effects determine pathologic changes,which may sustain chronic intestinal inflammation and also underlie specific disease *** addition to highlighting the potential use of DAMPs such as calprotectin as biomarkers,research on DAMPs may reveal novel mechanistic associations in IBD pathogenesis and is expected to uncover putative therapeutic targets.

关键词： Damage-associated molecular patterns Environmental factors Epigenetics Inflammatory bowel disease Therapeutic targets

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Tritrichomonas foetus:New structures by high-resolution scanning helium ion microscopy

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BIOCELL 2021年第2期45卷 259-266页

作者： MARLENE BENCHIMOL ABIGAIL MIRANDA-MAGALHÃES ANTONIO pereira-NEVES WANdeRLEY de souza Universidade do Grande Rio Duque de Caxias25071-202Rio de Janeiro departamento de Microbiologia Laboratório de Biologia Celular de PatógenosFiocruzInstituto Aggeu MagalhãesRecife52060-269Brazil Universidade Federal do Rio de Janeiro Instituto de Biofísica Carlos Chagas FilhoLaboratório de Ultraestrutura Celular Hertha Meyer Instituto Nacional de Ciência e Tecnologia(INBEB)and Centro Nacional de Biologia Estrutural e Bioimagens(CENABIO)Rio de Janeiro21941-902Brazil

Helium ion scanning microscopy(HIM)is a novel high-resolution scanning microscopy technique that uses helium ions instead of electrons to form images of the highest quality and resolution,providing a sub-nanometer resolution sputter uncoated biological ***,we took advantage of HIM to explore the cell surface of Tritrichomonas foetus,a protist parasite of cattle that provokes hard infection and abortion in *** describe thin protrusions,like nanotubes described in other cells,with different sizes(27 nm to 81 nm in thickness)and various lengths(from 73 nm to 2μm),as well bulbous structures either budding from the cell surface or present in the extremities of some *** flagella also presented these thin protrusions and different protein decoration,similar to those previously described using freeze-fracture *** between two cells were also seen,and their role in infection is *** cell surface was also examined and showed several pits indicative of endocytic activity and other types of arrays of *** observations were confirmed using Scanning Electron Microscopy(SEM),negative staining,and conventional thin sectioning for observation by transmission electron *** findings provide new and relevant information that may contribute to a better understanding of protozoan biology and its interaction with mammalian cells.

关键词： Nanotubes Microvesicles Surface specializations

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Dietary supplementation with free methionine or methionine dipeptide mitigates intestinal oxidative stress induced by Eimeria spp. challenge in broiler chickens

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Journal of Animal Science and Biotechnology 2019年第4期10卷 1005-1021页

作者： Angélica de souza Khatlab Ana Paula del Vesco Adhemar Rodrigues de Oliveira Neto Roberta pereira Miranda Fernandes Eliane Gasparino Animal Science department State University of MaringáColombo Avenue5790Jardim UniversitárioMaringáParaná87020-900Brazil Animal Science department Federal University of SergipeMarechal Rondon AvenueS/NJardim Rosa ElzeSao CristóvaoSergipe 49100-000Brazil EVONIK of Brazil Arquiteto Olavo Redig de Campos Street105Tower ASo PauloSP 04711-904Brazil Physiology departament Federal University of SergipeMarechal Rondon AvenueS/NJardim Rosa ElzeSao CristóvaoSergipe 49100-000Brazil

Background: This study evaluated the effects of Eimeria spp. challenge and dietary supplementation with free methionine or methionine dipeptide on animal performance;expression of genes associated with the immune system, antioxidant system, and amino acid transport in the jejunum;and redox status of the jejunum of broiler ***: A randomized, 2 × 3 factorial design was used, in which Eimeria spp. challenge was the first factor(Eimeria-challenged, EC, or unchallenged, UC, broilers) and methionine supplementation was the second factor(non-supplemented, NS;free DL-methionine, DL-Met;and methionine dipeptide, DL-methionyl-DL-methionine, DLMMet). At 14 days of age, chickens were inoculated orally with sporulated oocysts of Eimeria acervulina, Eimeria praecox, Eimeria maxima, and Eimeria mitis. Birds were killed by cervical dislocation 144 h post-inoculation(PI), and the jejunum was collected for biochemical and molecular ***: EC broilers had a 13% lower feed intake(FI), 37% lower body weight gain(BWG), and 39% higher feed conversion ratio(FCR) than UC broilers. Chickens fed the DL-Met diet had higher BWG(about 12% higher) and better FCR(about 12% lower) than chickens fed the NS diet. EC chickens had lower relative weight of the bursa of Fabricius(51.8%) and higher relative weights of the spleen and whole intestine(53.6% and 26.3%, respectively) than UC chickens. Eimeria spp. challenge led to an increase in the levels of oxidative substances, such as nitrite and thiobarbituric acid reactive substances(TBARS), in the jejunum of chickens 144 h PI. Among UC chickens, those fed the DL-Met diet had higher total antioxidant capacity(TAC) and lower catalase(CAT) and superoxide dismutase(SOD) activities. EC chickens that received the NS diet had higher carbonylated protein content(CP). This result was associated with their lower TAC and catalase activity. The lower TAC in EC chickens might have been due to reduced expression of catalase(CAT) and superoxide

关键词： Antioxidant Coccidiosis Gut INFG PEPT1

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